Anti-Proliferative Activity of Poloxamer Cobalt Ferrite Nanoparticles against Human Prostate Cancer (DU-145) Cells - In-Vitro Study

Sep 21, 2026

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Anti-proliferative effects of poloxamer-coated cobalt ferrite nanoparticles are evaluated against human prostate cancer DU-145 cells using in-vitro hyperthermia under a radiofrequency magnetic field. Cobalt ferrite nanoparticles are synthesized via the hydrothermal method and characterized for structure, size, and morphology, while cytotoxicity and RF-MF exposure are assessed separately and in combination. Cell viability is measured using MTT and colony formation assays, and TEM confirms spherical particles around 5.5×2.6 nm. RF-MF and nanoparticles alone do not markedly reduce viability, whereas their combination eradicates about 53% of cancerous cells.

LanguageEnglish

CategoryResearch & Report

FormatPDF

Pages8

Reading Time20 min

AccessFree to download

Introduction

Materials and Methods

Nanoparticle synthesis and characterization

Cell viability and cytotoxicity assays

Results

Morphology and heating behavior

Effects of nanoparticles and RF-MF alone or combined

Hindawi IET Nanobiotechnology Volume 2024, Article ID 8929168, 8 pages [https://doi.org/10.1049/2024/8929168](https://doi.org/10.1049/2024/8929168) Research Article Anti-Proliferative Activity of Poloxamer Cobalt Ferrite Nanoparticles against Human Prostate Cancer (DU-145) Cells: In-Vitro Study Nazanin Oroskhani, 1,2 Seyed Mohammad Amini ,2 Sakine Shirvalilou ,3 Mehdi Khodaie ,4 and Seyed Rabi Mahdavi 1,2 1Department of Medical Physics, School of Medicine, Iran University of Medical Sciences, Tehran, Iran 2Radiation Biology Research Center, Iran University of Medical Sciences, Tehran, Iran 3Finetech in Medicine Research Center, Iran University of Medical Sciences, Tehran, Iran 4Materials Science and Engineering, K. N. Toosi University of Technology, Tehran, Iran Correspondence should be addressed to Seyed Mohammad Amini; mohammadamini86@gmail.com and Seyed Rabi Mahdavi; [srmahdavi@hotmail.com](srmahdavi@hotmail.com) Received 21 June 2023; Revised 3 December 2023; Accepted 5 March 2024; Published 20 March 2024 Academic Editor: Anahita Haghizadeh Copyright © 2024 Nazanin Oroskhani et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Prostate cancer is the second most frequent type of cancer death in men. This study refers to the novel hyperthermia application of poloxamer-coated cobalt ferrite as a new approach for thermal eradication of DU-145 human prostate cancerous cells under a radio frequency magnetic ﬁeld (RF-MF) . The hydrothermal method was applied for the synthesis of cobalt ferrite nanoparticles. Then, the structure, size, and morphology of nanoparticle were characterized. The cytotoxicity of the synthesized nanoparticles and RF-MF exposure on DU-145 prostate cancer cells was investigated separately or in combination with colony formation methods and MTT [3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide] assay. Transmission electron microscopy (TEM) conﬁrmed the spherical morphology of nanoparticles with a size of 5.5 Æ 2.6nm. The temperature of cells treated with nanoparticles under RF-MF reached 42.73 Æ 0.2°C after 15min. RF-MF treatment or nanoparticles have not affected cell viability signiﬁcantly. However, the combination of them eradicated 53% Æ 4% of cancerous cells. In-vitro hyperthermia was performed on human prostate cancer cells (DU-145) with cobalt ferrite nanoparticles at speciﬁc concentrations that demonstrated a decrease in survival fraction based on colony formation assay compared to cells that were treated alone with nanoparticles or with RF-MF. 1. Introduction Eradicating cancer cells without harming healthy cells has been a primary aim in chemotherapy and radiation therapy. The side effect of chemotherapy and radiation treatment on normal tissue, multidrug resistance and radiation resistance, problems in biological issues to concentrate chemotherapy and radiation treatment of some tumors, etc. have reduced the treatment efﬁciency of prostate cancer [1] . It has been demonstrated that hyperthermia could enhance the chemoor radiation treatment of PCa [2] . Historically, heat has been transferred to cancerous cells using external applicators, either in the form of ultrasound, electric ﬁeld, or microwaves [3] . However, energy generated by these methods creates unwanted hotspots in normal tissue because it is difﬁcult to focus the energy on the target area [4] . As a result, thereis a continuing need for new hyperthermia application techniques with better heating properties [5] . Therefore, new approaches must be developed to supplement or replace existing procedures of treatment. Nowadays, there are various modern techniques based on nanoparticles that show fewer side effects, hyperthermia induced by an external magnetic ﬁeld (magnetic hyperthermia (MH)) is an example of them [5, 6] . MH uses ma

FAQs

How were the cobalt ferrite nanoparticles synthesized for the study?

Cobalt ferrite nanoparticles were synthesized using the hydrothermal method, then their structure, size, and morphology were characterized.

What assays were used to evaluate the effect on DU-145 cells?

Cytotoxicity and RF-MF exposure were investigated using colony formation methods and an MTT assay.

What was the effect of nanoparticles and RF-MF when used together compared with separately?

Nanoparticles or RF-MF alone did not significantly affect cell viability, while their combination eliminated about 53% of cancerous cells.

Sep 21, 2026

Uploaded by Ezra