An extensive analysis of the prognostic and immune role of FOXO1 in various types of cancer

Sep 21, 2026

Uploaded by Eliana

Forkhead Box O1 (FOXO1) has been reported to participate in multiple tumors, yet its roles across pan-cancer remain unclear. This study performed a pan-cancer analysis across 33 cancer types using multiple public platforms to define FOXO1 expression profiles, assess associations between FOXO1 expression and patient prognosis, and explore underlying mechanisms. FOXO1 correlated with prognosis in several cancers, notably LGG, OV, and KIRC, influencing immune microenvironment features and overall survival, with enrichment analyses highlighting immunoregulatory interactions and TGF-beta signaling.

LanguageEnglish

CategoryResearch & Report

FormatPDF

Pages12

Reading Time30 min

AccessFree to download

Abstract

Introduction

Material and Methods

Data sources

Brazilian Journal of Medical and Biological Research (2024) 57: e13378, [https://doi.org/10.1590/1414-431X2024e13378](https://doi.org/10.1590/1414-431X2024e13378) ISSN 1414-431X Research Article 1/12 An extensive analysis of the prognostic and immune role of FOXO1 in various types of cancer Jie Li 1,3* 00 , Chao Wang 1,3* 00 , Xiao Xu2,3 00 , Jun Chen 1,3 00 , and Haijun Guo 1,2,3* 00 1Department of Hepatobiliary and Pancreatic Surgery, Afﬁliated Hangzhou First People’s Hospital, West Lake University School of Medicine, Hangzhou, China 2Zhejiang University School of Medicine, Hangzhou, China 3Key Laboratory of Integrated Oncology and Intelligent Medicine of Zhejiang Province, Hangzhou, China Abstract Forkhead Box O1 (FOXO1) has been reported to play important roles in many tumors. However, FOXO1 has not been studied in pan-cancer. The purpose of this study was to reveal the roles of FOXO1 in pan-cancer (33 cancers in this study) . Through multiple public platforms, a pan-cancer analysis of FOXO1 was conducted to obtained FOXO1 expression proﬁles in various tumors to explore the relationship between FOXO1 expression and prognosis of these tumors and to disclose the potential mechanism of FOXO1 in these tumors. FOXO1 was associated with the prognosis of multiple tumors, especially LGG (low grade glioma), OV (ovarian carcinoma), and KIRC (kidney renal clear cell carcinoma) . FOXO1 might play the role of an oncogenic gene in LGG and OV, while playing the role of a cancer suppressor gene in KIRC. FOXO1 expression hada signiﬁcant correlation with the inﬁltration of some immune cells in LGG, OV, and KIRC. By combining FOXO1 expression and immune cell inﬁltration, we found that FOXO1 might inﬂuence the overall survival of LGG through the inﬁltration of myeloid dendritic cells or CD4 + T cells. Functional enrichment analysis and gene set enrichment analysis showed that FOXO1 might play roles in tumors through immunoregulatory interactions between a lymphoid and a non-lymphoid cell, TGF-beta signaling pathway, and transcriptional misregulation in cancer. FOXO1 was associated with the prognosis of multiple tumors, especially LGG, OV, and KIRC. In these tumors, FOXO1 might play its role via the regulation of the immune microenvironment. Key words: FOXO1; Prognostic analysis; Immune analysis; Pan-cancer Introduction The global incidence and death rates of cancer are increasing at an alarming rate (1) . Cancer is the primary reason for mortality and a major obstacle to improving lifespan in nations globally (2) . Finding new biomarkers to diagnose and treat cancers is urgent. FOXO1, the ﬁrst gene discovered in the FoxO family, functions as a transcriptional controller implicated in growth, programmed cell death, metabolic processes, and the reaction to stress (3) . FOXO1 has gained signiﬁcant interest in recent times as a promising molecular target for inhibiting cancer. According to reports, FOXO1 has signiﬁcant involvement in various types of cancer, such as hepatocellular cancer, pancreatic cancer, gastric cancer, and others (4–9) . However, the functions and processes of FOXO1 indifferent types of cancers remain incompletely understood. By analyzing extensive epigenomic, genomic, proteomic, and transcriptome data from various tumors published on multiple public platforms, a pan-cancer study can detect shared characteristics and variations in speciﬁc molecules across different types of cancer (10) . Pan-cancer analysis plays a crucial role in tumor diagnosis and treatment by artiﬁcially identifying the manifestation and altering characteristics of molecules across various tumors (11) . The aim of this study was to acquire FOXO1 expression patterns in diverse tumors, investigate the correlation between FOXO1 expression and tumor prognosis, and uncover the potential mechanisms of FOXO1 in various malignancies. Material and Methods Data sources The study included a total of 33 tumors, which are referred to

FAQs

What is the main purpose of this study on FOXO1?

To reveal FOXO1 roles across pan-cancer by characterizing expression profiles, evaluating links to prognosis, and uncovering potential mechanisms in multiple cancers.

Which cancer types showed particularly strong prognostic associations with FOXO1?

FOXO1 was associated with prognosis in multiple tumors, especially LGG (low grade glioma), OV (ovarian carcinoma), and KIRC (kidney renal clear cell carcinoma).

How might FOXO1 influence survival through the immune system?

Combining FOXO1 expression with immune cell infiltration suggested that FOXO1 may affect overall survival in LGG through infiltration of myeloid dendritic cells or CD4+ T cells.

Sep 21, 2026

Uploaded by Eliana