[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"detail-sidebar-cat-1-en-105":3,"doc-seo-571725-105":53,"doc-detail-571725-en":127},{"code":4,"msg":5,"data":6},0,"success",[7,14,19,24,29,34,39,44,49],{"id":8,"doc_module":9,"doc_module_name":10,"category_name":11,"show_sort_weight":12,"slug":13},11,1,"Template","Presentations",90,"presentations",{"id":15,"doc_module":9,"doc_module_name":10,"category_name":16,"show_sort_weight":17,"slug":18},12,"Resumes",80,"resumes",{"id":20,"doc_module":9,"doc_module_name":10,"category_name":21,"show_sort_weight":22,"slug":23},14,"Invoices",70,"invoices",{"id":25,"doc_module":9,"doc_module_name":10,"category_name":26,"show_sort_weight":27,"slug":28},15,"Posters",60,"posters",{"id":30,"doc_module":9,"doc_module_name":10,"category_name":31,"show_sort_weight":32,"slug":33},16,"Social Media",50,"social-media",{"id":35,"doc_module":9,"doc_module_name":10,"category_name":36,"show_sort_weight":37,"slug":38},17,"Forms",40,"forms",{"id":40,"doc_module":9,"doc_module_name":10,"category_name":41,"show_sort_weight":42,"slug":43},18,"Letters",30,"letters",{"id":45,"doc_module":9,"doc_module_name":10,"category_name":46,"show_sort_weight":47,"slug":48},21,"Paper Templates",5,"papers-templates",{"id":50,"doc_module":9,"doc_module_name":10,"category_name":51,"show_sort_weight":4,"slug":52},158,"General","general-158",{"code":4,"msg":54,"data":55},"ok",{"site_id":56,"language":57,"slug":58,"title":59,"keywords":60,"description":61,"schema_data":62,"social_meta":120,"head_meta":122,"extra_data":124,"updated_unix":126},105,"en","tube-chart-order-of-draw","Tube Chart - Order of Draw","","Tube chart and order-of-draw guidance for venous blood collection tubes, detailing cap colors, tube types, additives, additive numbers/inversions, and testing disciplines across coagulation, serum, chemistry, hematology, immunohematology, molecular diagnostics, viral markers, and glycolytic inhibition. Includes centrifugation recommendations by tube type with g-force and time, mixing and inversion instructions, specimen handling and draw volume guide for buffered sodium citrate, and collection tips to minimize cross-contamination and ensure correct blood-to-additive ratios. References CLSI standards and provides specimen and tourniquet handling cautions.",{"@graph":63,"@context":119},[64,80,102],{"@type":65,"itemListElement":66},"BreadcrumbList",[67,71,74,77],{"item":68,"name":69,"@type":70,"position":9},"https://docshare.wps.com","Home","ListItem",{"item":72,"name":10,"@type":70,"position":73},"https://docshare.wps.com/template/",2,{"item":75,"name":51,"@type":70,"position":76},"https://docshare.wps.com/template/general/",3,{"item":78,"name":59,"@type":70,"position":79},"https://docshare.wps.com/template/tube-chart-order-of-draw/571725/",4,{"url":78,"name":59,"@type":81,"image":82,"author":87,"headline":59,"publisher":90,"fileFormat":93,"inLanguage":57,"description":61,"dateModified":94,"datePublished":95,"encodingFormat":93,"isAccessibleForFree":96,"interactionStatistic":97},"DigitalDocument",{"url":83,"@type":84,"width":85,"height":86},"https://docshare.wps.com/thumbnails/tube-chart-order-of-draw/571725.png","ImageObject",442,249,{"name":88,"@type":89},"Connor ","Person",{"url":68,"name":91,"@type":92},"DocShare","Organization","application/pdf","2026-10-25","2026-10-13",true,{"@type":98,"interactionType":99,"userInteractionCount":101},"InteractionCounter",{"@type":100},"ViewAction",8,{"@type":103,"mainEntity":104},"FAQPage",[105,111,115],{"name":106,"@type":107,"acceptedAnswer":108},"What centrifugation g-force and time are recommended for common tube types?","Question",{"text":109,"@type":110},"The document lists VACUETTE serum, serum with clot activator and gel, K2EDTA with gel, plasma (heparin or PO/NaF), lithium heparin with gel, and coagulation tubes with specific g-force ranges and minutes for each.","Answer",{"name":112,"@type":107,"acceptedAnswer":113},"How should the order of draw be applied to avoid cross-contamination?",{"text":114,"@type":110},"When collecting multiple specimens in one venipuncture, follow the recommended sequence: (1) blood culture, (2) coagulation, (3) serum with/without clot activator or gel, (4) heparin tube with/without gel, (5) EDTA, and (6) glycolytic inhibitor, and follow facility protocol.",{"name":116,"@type":107,"acceptedAnswer":117},"How do you ensure the correct blood-to-additive ratio for coagulation testing?",{"text":118,"@type":110},"Check draw volume against the nominal fill mark or use the guide, and for hematocrit values greater than 55% adjust sodium citrate volume using the provided formula. Also invert tubes the required number of times for proper mixing.","https://schema.org",{"og:url":78,"og:type":121,"og:title":59,"og:site_name":91,"og:description":61},"article",{"robots":123,"canonical":78},"index,follow",{"doc_id":125,"site_id":56},571725,1792087355,{"code":4,"msg":5,"data":128},{"doc_id":125,"user_id":129,"nickname":88,"user_avatar":130,"doc_module":9,"category_id":50,"category_name":51,"doc_title":59,"doc_description":61,"doc_content":131,"file_id":132,"file_url":133,"file_type":134,"file_size":135,"view_count":101,"is_deleted":4,"is_public":9,"is_downloadable":9,"audit_status":9,"page_count":20,"language":136,"language_code":57,"site_id":56,"html_lang":57,"table_of_contents":137,"faqs":138,"seo_title":139,"seo_description":61,"update_tm":140,"read_time":47},687207022233,"https://ap-avatar.wpscdn.com/davatar_155a257f0dc6eb9ab79c44ca47cae57d","Tube Chart  \nVenous Blood Collection Tubes  \nCap Additive Number of Testing  \nColors Inversions Disciplines  \n\n| | No Additive* | 5-10 | Discard Tube\u003Cbr>Transport/Storage\u003Cbr>Immunohematology\u003Cbr>Viral Markers |\n| --- | --- | --- | --- |\n| | Sodium Citrate\u003Cbr>3.2%(0 .109 M)* | 4 | Coagulation |\n| | Clot Activator* | 5-10 | Chemistry\u003Cbr>Immunochemistry\u003Cbr>Immunohematology\u003Cbr>Viral Markers |\n| | Clot Activator w/Gel | 5-10 | Chemistry\u003Cbr>Immunochemistry\u003Cbr>TDMs |\n| \u003Cbr>| Lithium Heparin*\u003Cbr>Lithium Heparin w/Gel\u003Cbr>Sodium Heparin* | 5-10 | Chemistry\u003Cbr>Immunochemistry |\n| \u003Cbr>| K3 EDTA*\u003Cbr>K2 EDTA* | 8-10 | Hematology\u003Cbr>Immunohematology\u003Cbr>Molecular Diagnostics\u003Cbr>Viral Markers |\n\n* Also available in pediatric or low draw volumes of 2mL or less. Tech Services 1.800.515.8112  \n[www.gbo.com](www.gbo.com)  \nover for additional tubes   \n0724003R2  \nTube Chart  \nTube Chart  \nVenous Blood Collection Tubes  \n\n| Cap\u003Cbr>Colors |  | Additive | Number of |  |  | Testing |\n| --- | --- | --- | --- | --- | --- | --- |\n|  |  |  | Inversions |  |  | Disciplines |\n| | K2 EDTA Gel |  |  | 8-10 | Molecular Diagnostics |  |\n| | Potassium Oxalate /\u003Cbr>Sodium Fluoride* |  |  | 5-10 | Glycolytic Inhibitor\u003Cbr>Glucose and Lactate |  |\n| | Sodium Heparin\u003Cbr>No Additive |  |  | 5-10 | Trace Elements |  |\n\n* Also available in pediatric or low draw volumes of 2mL or less.  \nyellow  \nGel Separation  \nblack  \nStandard Draw  \ngreen  \nSodium Heparin  \nwhite  \nPediatric Draw  \nCENTRIFUGATION RECOMMENDATIONS  \n\n| tube type | g-force | minute |\n| --- | --- | --- |\n| VACUETTE® Serum Tubes (Clot Activator, No Additive) | Min. 1500 g | 10 |\n| VACUETTE® Serum Clot Activator w/ Gel Tubes | 1800 g | 10 |\n| VACUETTE® K2EDTA w/ Gel Tubes | 1800-2200 g | 10 |\n| VACUETTE® Plasma Tubes (Lithium or Sodium Heparin, PO/NaF) | 2000-3000 g | 15 |\n| VACUETTE® Lithium Heparin w/ Gel Tubes | 1800-2200 g | 10-15 |\n| VACUETTE® Coagulation Tubes (Sodium Citrate) |  |  |\n| Platelet tests (PRP) | 150 g | 5 |\n| Routine tests (PPP) | 1500-2000 g | 10 |\n| Preparation for deep freeze plasma (PFP) | 2500-3000 g | 20 |\n\nTech Services 1.800.515.8112 [www.gbo.com](www.gbo.com)  \nover for additional tubes   \n0724003R2  \nTube Chart  \nOrder of Draw  \nCLSI Recommended*  \nIf a winged blood collection set is used, the first tube in the series will be underfilled. Therefore, if a coagulation specimen is drawn first, a discard tube (a no additive or coagulation tube) is recommended to be drawn prior to this tube to ensure the proper anticoagulant-toblood ratio.  \nCap Color  \nTube  \nType  \nNo. of Inversions  \n Blue Coagulation 4   \nRed Serum 5-10  \nGreen Heparin 5-10   \nPink/Lavender EDTA 8-10  \n Grey Glycolytic Inhibitor 5-10   \nReproduced with permission from CLSI. GP41-A6, Procedures for the Collection of Diagnostic Blood Specimens by Venipuncture; Approved Standard-Sixth Edition. Copies of the current edition may be obtained from Clinical and Laboratory Standards Institute, 950 West Valley Road, Suite 2500, Wayne, Pennsylvania 19087-1898, USA. Internet: [www.clsi.org](www.clsi.org).  \nTech Services 1.800.515.8112  \n[www.gbo.com](www.gbo.com 0724003R2)[ 0724003R2](www.gbo.com 0724003R2)  \nOrder of Draw  \nCompleting an Inversion  \nTo achieve the proper mix of additive and blood, each tube must be gently inverted as it is removed from the holder.  \nOne complete inversion   \nTurn the filled tube upside down and return it to an upright position  \n Repeat required number of times for each tube type (see reverse for \\# of inversions)  \n1 Inversion  \nImportance of Mixing  \n Insufficient or delayed mixing of serum tubes may result in delayed clotting.  \n Inadequate mixing of anticoagulant tubes may result in platelet clumping, clotting or incorrect test results.  \nTech Services 1.800.515.8112  \n[www.gbo.com](www.gbo.com 0724003R2)[ 0724003R2](www.gbo.com 0724003R2)  \nSpecimen Handling  \nCoagulation Draw Volume Guide  \nTake the Guesswork Out of It  \nEnsure that the correct blood-to-additive ratio is met by chec","cbCaiaaTRjcjQN3j","https://ap.wps.com/l/cbCaiaaTRjcjQN3j","pdf",1207333,"English","# Tube Chart\n## Venous Blood Collection Tubes\n## Cap Colors, Additives, and Testing Disciplines\n# Centrifugation Recommendations\n## VACUETTE Tube Types, g-force, and Time\n# Order of Draw (CLSI Recommended)\n## Discard Tube Guidance and Cap Color Mapping\n# Completing an Inversion & Importance of Mixing\n# Specimen Handling\n## Coagulation Draw Volume Guide\n## Collection Tips for Coagulation Testing\n## Tourniquet Use and Hematocrit Adjustment","[{\"question\":\"What centrifugation g-force and time are recommended for common tube types?\",\"answer\":\"The document lists VACUETTE serum, serum with clot activator and gel, K2EDTA with gel, plasma (heparin or PO/NaF), lithium heparin with gel, and coagulation tubes with specific g-force ranges and minutes for each.\"},{\"question\":\"How should the order of draw be applied to avoid cross-contamination?\",\"answer\":\"When collecting multiple specimens in one venipuncture, follow the recommended sequence: (1) blood culture, (2) coagulation, (3) serum with/without clot activator or gel, (4) heparin tube with/without gel, (5) EDTA, and (6) glycolytic inhibitor, and follow facility protocol.\"},{\"question\":\"How do you ensure the correct blood-to-additive ratio for coagulation testing?\",\"answer\":\"Check draw volume against the nominal fill mark or use the guide, and for hematocrit values greater than 55% adjust sodium citrate volume using the provided formula. Also invert tubes the required number of times for proper mixing.\"}]","Tube Chart - Order of Draw | PDF",1791923406]