[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"detail-sidebar-cat-1-en-105":3,"doc-seo-564861-105":53,"doc-detail-564861-en":127},{"code":4,"msg":5,"data":6},0,"success",[7,14,19,24,29,34,39,44,49],{"id":8,"doc_module":9,"doc_module_name":10,"category_name":11,"show_sort_weight":12,"slug":13},11,1,"Template","Presentations",90,"presentations",{"id":15,"doc_module":9,"doc_module_name":10,"category_name":16,"show_sort_weight":17,"slug":18},12,"Resumes",80,"resumes",{"id":20,"doc_module":9,"doc_module_name":10,"category_name":21,"show_sort_weight":22,"slug":23},14,"Invoices",70,"invoices",{"id":25,"doc_module":9,"doc_module_name":10,"category_name":26,"show_sort_weight":27,"slug":28},15,"Posters",60,"posters",{"id":30,"doc_module":9,"doc_module_name":10,"category_name":31,"show_sort_weight":32,"slug":33},16,"Social Media",50,"social-media",{"id":35,"doc_module":9,"doc_module_name":10,"category_name":36,"show_sort_weight":37,"slug":38},17,"Forms",40,"forms",{"id":40,"doc_module":9,"doc_module_name":10,"category_name":41,"show_sort_weight":42,"slug":43},18,"Letters",30,"letters",{"id":45,"doc_module":9,"doc_module_name":10,"category_name":46,"show_sort_weight":47,"slug":48},21,"Paper Templates",5,"papers-templates",{"id":50,"doc_module":9,"doc_module_name":10,"category_name":51,"show_sort_weight":4,"slug":52},158,"General","general-158",{"code":4,"msg":54,"data":55},"ok",{"site_id":56,"language":57,"slug":58,"title":59,"keywords":60,"description":61,"schema_data":62,"social_meta":120,"head_meta":122,"extra_data":124,"updated_unix":126},105,"en","truforma-point-of-care-canine-progesterone-assay-white-paper-3-13-25","TRUFORMA Point of Care Canine Progesterone Assay - White Paper - 3-13-25","","TRUFORMA Point of Care Canine Progesterone Assay presents an approach to accurately and precisely measure endogenous serum progesterone (P4) for veterinary decision-making at the clinic. The platform uses bulk acoustic wave (BAW) sensing to enable rapid, nonoptical and fluorescence-free detection without relying on time-consuming reference laboratory workflows. The assay supports quantification across a dynamic range for use throughout the estrus cycle and pregnancy, and provides high precision with strong correlation to reference laboratory results.",{"@graph":63,"@context":119},[64,80,102],{"@type":65,"itemListElement":66},"BreadcrumbList",[67,71,74,77],{"item":68,"name":69,"@type":70,"position":9},"https://docshare.wps.com","Home","ListItem",{"item":72,"name":10,"@type":70,"position":73},"https://docshare.wps.com/template/",2,{"item":75,"name":51,"@type":70,"position":76},"https://docshare.wps.com/template/general/",3,{"item":78,"name":59,"@type":70,"position":79},"https://docshare.wps.com/template/truforma-point-of-care-canine-progesterone-assay-white-paper-3-13-25/564861/",4,{"url":78,"name":59,"@type":81,"image":82,"author":87,"headline":59,"publisher":90,"fileFormat":93,"inLanguage":57,"description":61,"dateModified":94,"datePublished":95,"encodingFormat":93,"isAccessibleForFree":96,"interactionStatistic":97},"DigitalDocument",{"url":83,"@type":84,"width":85,"height":86},"https://docshare.wps.com/thumbnails/truforma-point-of-care-canine-progesterone-assay-white-paper-3-13-25/564861.png","ImageObject",442,249,{"name":88,"@type":89},"EvelynWrites","Person",{"url":68,"name":91,"@type":92},"DocShare","Organization","application/pdf","2026-11-01","2026-10-12",true,{"@type":98,"interactionType":99,"userInteractionCount":101},"InteractionCounter",{"@type":100},"ViewAction",9,{"@type":103,"mainEntity":104},"FAQPage",[105,111,115],{"name":106,"@type":107,"acceptedAnswer":108},"Why is a point-of-care canine progesterone assay important for breeding and parturition?","Question",{"text":109,"@type":110},"Current reference laboratory immunoassays take time and often require semi-invasive examination. A reliable POC assay supports precise breeding timing and prediction of parturition without those delays.","Answer",{"name":112,"@type":107,"acceptedAnswer":113},"How does TRUFORMA detect progesterone at the point of care?",{"text":114,"@type":110},"TRUFORMA uses bulk acoustic wave (BAW) sensor technology to provide a nonoptical and fluorescence-free detection system for diagnostic use in veterinary clinics.",{"name":116,"@type":107,"acceptedAnswer":117},"What progesterone changes help determine the optimal breeding window?",{"text":118,"@type":110},"A rise from baseline to above 2.0 ng/mL indicates the LH surge, with ovulation typically about 2 days later. Progesterone often rises above 5.0 ng/mL and continues upward, guiding breeding plans based on day-relative values.","https://schema.org",{"og:url":78,"og:type":121,"og:title":59,"og:site_name":91,"og:description":61},"article",{"robots":123,"canonical":78},"index,follow",{"doc_id":125,"site_id":56},564861,1792285814,{"code":4,"msg":5,"data":128},{"doc_id":125,"user_id":129,"nickname":88,"user_avatar":130,"doc_module":9,"category_id":50,"category_name":51,"doc_title":59,"doc_description":61,"doc_content":131,"file_id":132,"file_url":133,"file_type":134,"file_size":135,"view_count":101,"is_deleted":4,"is_public":9,"is_downloadable":9,"audit_status":9,"page_count":136,"language":137,"language_code":57,"site_id":56,"html_lang":57,"table_of_contents":138,"faqs":139,"seo_title":140,"seo_description":61,"update_tm":141,"read_time":76},687212989558,"https://ap-avatar.wpscdn.com/davatar_a8503ba1806abce46bf441b54a3ca4cd","TRUFORMA® Point of Care  \nCanine Progesterone Assay  \nDane Anderson, Sr. Research and Development Scientist, Zomedica Dannyalle Breeden, Research and Development Scientist, Zomedica  \nT.J. Barclay, DVM, Sr. Professional Services Veterinarian, Zomedica  \n\n| Key Messages |\n| --- |\n| • Accurate and precise measurement of endogenous serum Progesterone (P4) levels is needed to manage timing of breeding, predict parturition in bitches, and identify reproductive disorders.\u003Cbr>• The TRUFORMA platform uses innovative bulk acoustic wave (BAW) technology to provide a nonoptical and fluorescence-free detection system for diagnostic use atthe point of care (POC) in veterinary clinics.\u003Cbr>• The dynamic range of the TRUFORMA Canine Progesterone assay allows for the quantification of high and low concentrations of serum P4, enabling use during the entire estrus cycle and pregnancy of the patient.\u003Cbr>• The high precision and correlation to a reference laboratory assay shown fortheTRUFORMA Canine Progesterone assay provides veterinarians with accurate and reliable diagnostic results atthe POC, creating opportunities for improved patient care and real-time client communication. |\n\nIntroduction  \nAccurately monitoring breeding and parturition is challenging in veterinary medicine due to the time to result of current reference laboratory immunoassay methodologies and the need for semi-invasive examination of the patient. A reliable POC canine progesterone assay alleviates these challenges and ensures the precise timing needed for breeding and parturition. The TRUFORMA platform fits this need by using BAW sensor technology to provide veterinarians with rapid, reliable, and accurate measurement of progesterone levels at the POC.  \nThe objectives of this study were to:  \n• Determine analytical performance attributes fortheTRUFORMA Canine Progesterone assay.  \n• Describe how the TRUFORMA Canine Progesterone assay differs from other currently available assays.  \n• Compare the TRUFORMA Canine Progesterone assay performance with an assay used as part of the standard of care at veterinary diagnostic laboratories.  \nClinical Significance of Canine Progesterone  \nProgesterone is produced by ovarian corpora lutea (CL) and its primary function is the maintenance of pregnancy. Ovulation of ovarian follicles is triggered by a surge of luteinizing hormone (LH) from the pituitary gland. Unlike in other domestic mammals, the LH surge in bitches stimulates preovulatory luteinization of granulosa cells, causing progesterone levels to rise prior to ovulation. Progesterone levels continue to rise after ovulation as the CL matures. Maintenance of pregnancy in bitches is dependent upon ovarian production of progesterone for the entirety of gestation. Gestation length is 63 days, timed from the day of ovulation. Progesterone levels drop precipitously 24 – 48 hours prior to parturition.  \n\n| Figure 1. Progesterone Profile of Canine Estrus Cycle |  |  |\n| --- | --- | --- |\n| Progesterone (ng/ml) | 30\u003Cbr>25\u003Cbr>20\u003Cbr>15\u003Cbr>10\u003Cbr>5\u003Cbr>0 | \u003Cbr>\u003Cbr>\u003Cbr>-6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7 8\u003Cbr>\u003Cbr>\u003Cbr>\u003Cbr>Days relative to LH |\n\nLH, Luteinizing Hormone  \nCanine Progesterone Testing    \nProgesterone Analysis for Timing of Breeding  \nDetermination of the optimal breeding time in the bitch is predicated upon detecting the LH surge. Since the LH surge causes preovulatory luteinization of ovarian follicles, timing of the surge can be accomplished by measuring serum progesterone levels.  \nSerial progesterone measurements, starting atthe onset ofserosanguinous vulvar discharge and continued until breeding is complete, are recommended. In proestrus/early estrus, measurements taken every 2 – 4 days are usually adequate. After the LH surge, measurements should betaken every 2 days to ensure the expected rise in progesterone has taken place.1  \nA rise in progesterone from baseline to above 2.0 ng/mL indicates that the LH surge has occurred. Ovulation generally occurs 2 days later and is accom","cbCaic5lisQWjrRk","https://ap.wps.com/l/cbCaic5lisQWjrRk","pdf",434210,8,"English","# Key Messages\n# Introduction\n# Clinical Significance of Canine Progesterone\n# Canine Progesterone Testing\n## Progesterone Analysis for Timing of Breeding\n## Progesterone Analysis for Predicting Onset of Parturition\n# TRUFORMA Platform","[{\"question\":\"Why is a point-of-care canine progesterone assay important for breeding and parturition?\",\"answer\":\"Current reference laboratory immunoassays take time and often require semi-invasive examination. A reliable POC assay supports precise breeding timing and prediction of parturition without those delays.\"},{\"question\":\"How does TRUFORMA detect progesterone at the point of care?\",\"answer\":\"TRUFORMA uses bulk acoustic wave (BAW) sensor technology to provide a nonoptical and fluorescence-free detection system for diagnostic use in veterinary clinics.\"},{\"question\":\"What progesterone changes help determine the optimal breeding window?\",\"answer\":\"A rise from baseline to above 2.0 ng/mL indicates the LH surge, with ovulation typically about 2 days later. Progesterone often rises above 5.0 ng/mL and continues upward, guiding breeding plans based on day-relative values.\"}]","TRUFORMA Point of Care Canine Progesterone Assay - White Paper - 3-13-25 | PDF",1791844643]