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It includes specific draw order rules, mixing and handling guidance (including cautions for serum trace element collection), and standardized centrifugation/separation steps. The guide also details tube purpose for coagulation assays, serum chemistry, plasma chemistry, whole blood testing, blood bank testing, and urine chemistry, urinalysis, and culture workflows, plus references to associated phlebotomy and LIS downtime procedures.",{"@graph":63,"@context":112},[64,80,95],{"@type":65,"itemListElement":66},"BreadcrumbList",[67,71,74,77],{"item":68,"name":69,"@type":70,"position":9},"https://docshare.wps.com","Home","ListItem",{"item":72,"name":10,"@type":70,"position":73},"https://docshare.wps.com/template/",2,{"item":75,"name":36,"@type":70,"position":76},"https://docshare.wps.com/template/forms/",3,{"item":78,"name":59,"@type":70,"position":79},"https://docshare.wps.com/template/test-tube-guide-and-order-of-draw/571726/",4,{"url":78,"name":59,"@type":81,"author":82,"headline":59,"publisher":85,"fileFormat":88,"inLanguage":57,"description":61,"dateModified":89,"datePublished":89,"encodingFormat":88,"isAccessibleForFree":90,"interactionStatistic":91},"DigitalDocument",{"name":83,"@type":84},"Kyle","Person",{"url":68,"name":86,"@type":87},"DocShare","Organization","application/pdf","2026-10-13",true,{"@type":92,"interactionType":93,"userInteractionCount":4},"InteractionCounter",{"@type":94},"ViewAction",{"@type":96,"mainEntity":97},"FAQPage",[98,104,108],{"name":99,"@type":100,"acceptedAnswer":101},"What is the urine order of draw guideline in this guide?","Question",{"text":102,"@type":103},"The guide specifies an order of draw entry for urine, listing the associated tube/additive setup and the laboratory tests included under urine chemistry workflows.","Answer",{"name":105,"@type":100,"acceptedAnswer":106},"How should serum be collected for trace element testing?",{"text":107,"@type":103},"The guide instructs to carefully pour serum and not pipet it into a serum trace element vial, following the listed tube guidance for trace element panels.",{"name":109,"@type":100,"acceptedAnswer":110},"What mixing and handling steps are emphasized for blood collection tubes?",{"text":111,"@type":103},"It emphasizes mixing tubes by inverting 8 to 10 times and includes procedure steps for clot formation timing and subsequent spinning/separation where applicable.","https://schema.org",{"og:url":78,"og:type":114,"og:title":59,"og:site_name":86,"og:description":61},"article",{"robots":116,"canonical":78},"index,follow",{"doc_id":118,"site_id":56},571726,1791923408,{"code":4,"msg":5,"data":121},{"doc_id":118,"user_id":122,"nickname":83,"user_avatar":123,"doc_module":9,"category_id":35,"category_name":36,"doc_title":59,"doc_description":61,"doc_content":124,"file_id":125,"file_url":126,"file_type":127,"file_size":128,"view_count":4,"is_deleted":4,"is_public":9,"is_downloadable":9,"audit_status":9,"page_count":9,"language":129,"language_code":57,"site_id":56,"html_lang":57,"table_of_contents":130,"faqs":131,"seo_title":132,"seo_description":61,"update_tm":119,"read_time":4},3985741905716,"https://ap-avatar.wpscdn.com/davatar_994ba38a5ba835b3df7d355c54d3ed8d","Test Tube Guide and Order of Draw  \n| Test\u003Cbr>Tube |  |  | Alternate\u003Cbr>Test\u003Cbr>Tube | Additive | Vol. | Epro\u003Cbr>Item \\# | Laboratory Use |\n| --- | --- | --- | --- | --- | --- | --- | --- |\n| \u003Cbr>ORDER\u003Cbr>*\u003Cbr>OF\u003Cbr>*\u003Cbr>DRAW\u003Cbr>URINE | blood\u003Cbr>cultures | |  | Bactec Aerobic grey cap\u003Cbr>Bactec Anerobic orange cap\u003Cbr>Bactec Peds pink cap |  | 9617\u003Cbr>10595\u003Cbr>9616 | Make certain to follow the blood culture procedure\u003Cbr>using ChloraPrep One-Step - Item \\#22960\u003Cbr>Mix bottles by inverting 8 to 10 times. |\n|  | royal blue | |  | Trace Element Serum | 6 mL | see lab\u003Cbr>supply\u003Cbr>request | Serum Trace element test: chromium, cobalt, copper, selenium Serum: Carefully pour, do not pipet, serum into a serum trace element vial.\u003Cbr>|\n|  | blue | | *note below | Sodium Citrate Tube\u003Cbr>Fill test tube completely\u003Cbr>Proper mixing prevents clot formation | 4.5 mL | 89 | Coagulation testing. PT, PTT, TPT, Protein S, Protein C, Anti-Thrombin III, Lupus Anti-Coagulant |\n|  | red | | | Serum Tube with clot activator\u003Cbr>Allow 30 min. for clot formation\u003Cbr>Spin and separate serum into a plastic vial | 4 mL\u003Cbr>10 mL | 2219\u003Cbr>2222 | Serum chemistry tests: Digoxin, Dilantin, Drug Levels, Homocystine, Lithium, Tegretol, Theophylline,\u003Cbr>PTH Intact (Quest) |\n|  | gold | | | SST Gel Separator w/ clot activator Allow 30 minutes for clot formation OR Red tiger top tube. | 5 mL | 1234 | Serum chemistry tests: CA125, CA15-3, CA19-9, Free-PSA (sendout), Hepatitis, T3, T4, Free T4, TSH, FSH, LH, B12, B12 Folate |\n|  | [dk.green](dk.green) | | | Sodium Heparin Tube | 10 mL | 94 | Amino Acids, HLA B27, Comprehensive Drug Screen, Flow Cytometry |\n|  | green | | | Lithium Heparin PST Separator Tube OR Green tiger top tube.\u003Cbr>Proper mixing prevents clot formation | 4.5 mL | 2033 | Plasma chemistry tests: Ammonia, BMP, CMP Electrolytes, PTH Intact (NON-OR specimen), Lipid Profile Hepatic Function, Homocystine, PSA (Guthrie) |\n|  | dk green | |  | Lithium Heparin without gel | 9 mL | see lab\u003Cbr>supply\u003Cbr>request | T-SPOT |\n|  | lavender | |  | EDTA Tube\u003Cbr>Proper mixing prevents clot formation | 3 mL | 1012 | Primarily Whole blood testing: CBC, Glyco, Retic, Sed. Rate Mono, RBC Folate, Cyclosporin, Histamine, Cystic Fibrosis (INTRAOPERATIVE PTH-Operating Room. Use Only) |\n|  | pink | |  | K2 EDTA | 6 mL | 40075 | Whole blood blood bank tests: Type and Screen, Type and Cross, Direct Antiglobulin (DAT) |\n|  | royal blue | |  | K2 EDTA Trace Element Plasma and/or\u003Cbr>Whole Blood | 5 mL | see lab\u003Cbr>supply\u003Cbr>request | Trace elements: arsenic, cadmium, heavy metal screen, zinc Plasma: Carefully pour, do not pipet, plasma into a plasma trace element vial.\u003Cbr>|\n|  | white | |  | K2 EDTA | 6 mL | see lab\u003Cbr>supply\u003Cbr>request | Hepatitis C-Heptimax |\n|  | gray | | | Sodium Fluoride Potassium Oxalate | 5 mL | see lab\u003Cbr>supply\u003Cbr>request | Alcohol, Lactic Acid |\n|  | yellow | |  | ACD solution B | 6 mL | see lab\u003Cbr>supply\u003Cbr>request | HLA typing-oncology patients- \\# of tubes depends on the patient's WBC couhnt. Call the Blood Bank for assistance. (570) 887-4218 |\n|  | urine\u003Cbr>chemistry | |  | No additive, urine chemistry only | 6 mL | 70835 | Urine microalbumin, urine drug screen, urine osmolality, urine chemisty tests. |\n|  | urinalysis | |  | No additive, conical tube urinalysis only | 8 mL | 17522\u003Cbr>28279-tube & straw kit | \"V\" shaped conical tube is used for urinalysis testing only. The cone shape allows for microscopic examination. Urinalysis with refelx culture requires 2 test tubes, a conical and preservative tube. |\n|  | urine\u003Cbr>culture | |  | Preservative, urine culture, legionella | 4 mL | 63417\u003Cbr>17496-tube & straw kit | Urine culture. Store and transport at room temperature up to 48 Hours. |\n\n* When using a winged blood collection set is used and the first tube to be drawn is a citrate coagulation tube, a discard tube (non additive tube) should be drawn first. The discard tube is used to fill the \"dead space\" in the tubing to ensure proper blood-to-additive","cbCaiko2Lir6dTDe","https://ap.wps.com/l/cbCaiko2Lir6dTDe","pdf",94148,"English","# Test Tube Guide and Order of Draw\n## Collection tube mapping and laboratory use\n## Draw order, mixing, and handling notes","[{\"question\":\"What is the urine order of draw guideline in this guide?\",\"answer\":\"The guide specifies an order of draw entry for urine, listing the associated tube/additive setup and the laboratory tests included under urine chemistry workflows.\"},{\"question\":\"How should serum be collected for trace element testing?\",\"answer\":\"The guide instructs to carefully pour serum and not pipet it into a serum trace element vial, following the listed tube guidance for trace element panels.\"},{\"question\":\"What mixing and handling steps are emphasized for blood collection tubes?\",\"answer\":\"It emphasizes mixing tubes by inverting 8 to 10 times and includes procedure steps for clot formation timing and subsequent spinning/separation where applicable.\"}]","Test Tube Guide and Order of Draw | PDF"]