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It lists special instructions for complex testing such as blood cultures, coagulation studies, SST II applications, emergency chemistry, and specialized PCR and immunophenotyping workflows. 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No.  \nTube  \nType  \nDeterminations  \nSpecial Instructions  \n|  |  | Blood Cultures | Aerobic followed by anaerobic. If insufficient blood for both culture bottles, use aerobic bottle only. |  |\n| --- | --- | --- | --- | --- |\n|  | Cat. No.\u003Cbr>363095\u003Cbr>Draw Volume\u003Cbr>2.7ml | Sodium\u003Cbr>Citrate | INR, Clotting Screen and Factor Assays.\u003Cbr>Additional bottles required for Lupus\u003Cbr>Anticoagulant/Thrombophilia Screen. | Draw volume of 2. 7ml essential for 9:1 ratio of blood to anticoagulant. Not suitable for Immunology. |\n|  | Cat. No.\u003Cbr>367954 / 367956\u003Cbr>Draw Volume\u003Cbr>5ml / 3. 5ml | SST™ II\u003Cbr>Advance | U&E, LFT’s, Ca2+CRP Haematinics, Hormones,\u003Cbr>Theraputic Drug Monitoring. Routine Immunology, Serology and Virology antibody tests. Antibiotic Assays. Hepatitis, PCR, HCV Viral load and HCV Genotyping.\u003Cbr>***Calcitonin (on ice) . | *** Calcitonin can be in SST or Red top tube, but take/send on ice. |\n|  | Cat. No.\u003Cbr>367885\u003Cbr>Draw Volume\u003Cbr>6ml | Lithium\u003Cbr>Heparin | Emergency Chemistry, Plasma Renin, Plasma Aldosterone,\u003Cbr>Carboxyhaemoglobin. | Not suitable for any immunology. |\n|  | Cat. No.\u003Cbr>367839\u003Cbr>Draw Volume\u003Cbr>4ml | EDTA | Full blood count, ESR, Retics, HbA1C, Plasma viscosity, ZPP, Haemoglobinopathy, G6PD, Thalassaemia/Sickle Cell Screen, CD4 Monitoring, Immunophenotyping/Cell Markers, ***Haptoglobin/Haemolysis Screen, Glandular Fever Screen. *ACTH (on ice), TPMT, PTH (parathyroid hormone), **Viral Load. Meningococcal PCR, Pneumococcal PCR, Viral PCR (CMV, EBV, Adenovirus, BKV) Cyclosporin, Tacrolimus, Plasma Mentanephrins (on ice) . | * ACTH-requires immediate delivery to lab.\u003Cbr>** Viral Load-except Hep C.\u003Cbr>*** Haptoglobin/Haemolysis Screen requires both SST and EDTA samples. |\n|  | Cat. No.\u003Cbr>367941\u003Cbr>Draw Volume\u003Cbr>6ml | EDTA\u003Cbr>Crossmatch | Group and Save Serum, Crossmatch, Coombs Test. |  |\n|  | Cat. No.\u003Cbr>368201\u003Cbr>Draw Volume\u003Cbr>5ml | Fluoride\u003Cbr>Heparin | Glucose, Lactate, Alcohol. | Not suitable for any immunology. |\n\nMix  \n3-4 Times  \nMix  \n5-6 Times  \nMix  \n8-10 Times  \nMix  \n8-10 Times  \nMix  \n8-10 Times  \nMix  \n8-10 Times  \nDeterminations and Special Instructions contained within this guide have been provided by the named institute and are not BD recommendations or instructions for the BD products described. Please consult your organisation’s guidelines or contact BD should you have any questions.  \n*Clinical and Laboratory Standards Institute (CLSI) Guidelines GP41-Ed7 (formerly H3-A6, 6th Edition)  \nIMPORTANT MIXING GUIDELINES  \nAll BD Vacutainer® tubes require immediate mixing following collection. Insufficient mixing can result in inaccurate test results and the need tore-draw. Correct mixing technique is to gently invert (180o and back) each tube the recommended number of times shown on the right hand side of the table.  \n0118 921 6000  \n1030 Eskdale Rd, Winnersh, Wokingham RG41, UK  \n[bd.com/en-uk](bd.com/en-uk)  \n©2019 BD. BD, the BD Logo and all other trademarks are property of Becton, Dickinson and Company.  \nWEU227 .1 March 2019  \nSheffield Teaching Hospitals  \nNHS Foundation Trust","cbCaimTnLHkQhRuV","https://ap.wps.com/l/cbCaimTnLHkQhRuV","pdf",824069,"English","# Tube types and recommended order of draw\n## Special instructions for determinations\n## Important mixing guidelines","[{\"question\":\"How should BD Vacutainer tubes be mixed after collection?\",\"answer\":\"Mix immediately following collection by gently inverting each tube (180° and back) the recommended number of times shown in the table to avoid inaccurate results or the need to re-draw.\"},{\"question\":\"What is the correct approach for blood cultures if there isn’t enough blood for both bottles?\",\"answer\":\"Use aerobic bottle only if there is insufficient blood for both culture bottles; otherwise draw aerobic followed by anaerobic.\"},{\"question\":\"Are there any tests that require special temperature handling?\",\"answer\":\"Yes. Calcitonin must be taken/sent on ice, and ACTH requires immediate delivery to the lab. Viral load handling is also specified, with exceptions noted.\"}]","Sheffield Laboratory Medicine Tube Guide - Recommended Order of Draw | PDF",1791937243]