[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"doc-seo-195328-105":3,"detail-sidebar-cat-1-en-105":80,"doc-detail-195328-en":126},{"code":4,"msg":5,"data":6},0,"ok",{"site_id":7,"language":8,"slug":9,"title":10,"keywords":11,"description":12,"schema_data":13,"social_meta":73,"head_meta":75,"extra_data":77,"updated_unix":79},105,"en","plasmid-dna-template","Plasmid DNA Template","","This document describes a method for determining the genetic phase of a patient's DNA, likely for diagnostic or research purposes. The methodology involves several steps, starting with pouring a polony gel and amplifying colonies from patient DNA. The process then proceeds to genotype Single Nucleotide Polymorphisms (SNPs) using a Sequencing By Hybridization (SBE) technique. Specifically, two SNPs are genotyped sequentially. After the first SNP is genotyped, primers are stripped, and the second SNP is genotyped. Images of the colonies are then overlaid. Finally, the genetic phase is determined, which refers to the specific arrangement of alleles on each chromosome. The document includes a schematic of a plasmid DNA template with EcoRI and NcoI cut sites, indicating a region of 2100 bp. Images A and B show fluorescently labeled polonies, with red representing Locus A, green representing Locus B, and yellow representing overlapping colonies of both loci. Image C provides a legend for these representations. The overall technique appears to be a high-throughput method for analyzing genetic variation at specific loci.",{"@graph":14,"@context":72},[15,34,55],{"@type":16,"itemListElement":17},"BreadcrumbList",[18,23,27,31],{"item":19,"name":20,"@type":21,"position":22},"https://docshare.wps.com","Home","ListItem",1,{"item":24,"name":25,"@type":21,"position":26},"https://docshare.wps.com/template/","Template",2,{"item":28,"name":29,"@type":21,"position":30},"https://docshare.wps.com/template/general/","General",3,{"item":32,"name":10,"@type":21,"position":33},"https://docshare.wps.com/template/plasmid-dna-template/195328/",4,{"url":32,"name":10,"@type":35,"image":36,"author":41,"headline":10,"publisher":44,"fileFormat":47,"inLanguage":8,"description":12,"dateModified":48,"datePublished":49,"encodingFormat":47,"isAccessibleForFree":50,"interactionStatistic":51},"DigitalDocument",{"url":37,"@type":38,"width":39,"height":40},"https://docshare.wps.com/thumbnails/plasmid-dna-template/195328.png","ImageObject",442,249,{"name":42,"@type":43},"Riley West","Person",{"url":19,"name":45,"@type":46},"DocShare","Organization","application/pdf","2026-09-28","2026-09-03",true,{"@type":52,"interactionType":53,"userInteractionCount":33},"InteractionCounter",{"@type":54},"ViewAction",{"@type":56,"mainEntity":57},"FAQPage",[58,64,68],{"name":59,"@type":60,"acceptedAnswer":61},"What is the purpose of the plasmid DNA template shown in the document?","Question",{"text":62,"@type":63},"The plasmid DNA template diagram illustrates the structure of a circular DNA molecule with specific cut sites (EcoRI and NcoI) and a defined region of 2100 bp, serving as a reference for genetic analysis.","Answer",{"name":65,"@type":60,"acceptedAnswer":66},"How are different loci differentiated in the polony images?",{"text":67,"@type":63},"Different loci are differentiated by fluorescent labels: red indicates Locus A polony, green indicates Locus B polony, and yellow indicates overlapping polonies containing both Locus A and Locus B.",{"name":69,"@type":60,"acceptedAnswer":70},"What is the role of Sequencing By Hybridization (SBE) in this method?",{"text":71,"@type":63},"Sequencing By Hybridization (SBE) is used to genotype Single Nucleotide Polymorphisms (SNPs) by detecting specific sequences within the amplified colonies. It is applied sequentially for two different SNPs.","https://schema.org",{"og:url":32,"og:type":74,"og:title":10,"og:site_name":45,"og:description":12},"article",{"robots":76,"canonical":32},"index,follow",{"doc_id":78,"site_id":7},195328,1788447420,{"code":4,"msg":81,"data":82},"success",[83,88,93,98,103,108,113,118,123],{"id":84,"doc_module":22,"doc_module_name":25,"category_name":85,"show_sort_weight":86,"slug":87},11,"Presentations",90,"presentations",{"id":89,"doc_module":22,"doc_module_name":25,"category_name":90,"show_sort_weight":91,"slug":92},12,"Resumes",80,"resumes",{"id":94,"doc_module":22,"doc_module_name":25,"category_name":95,"show_sort_weight":96,"slug":97},14,"Invoices",70,"invoices",{"id":99,"doc_module":22,"doc_module_name":25,"category_name":100,"show_sort_weight":101,"slug":102},15,"Posters",60,"posters",{"id":104,"doc_module":22,"doc_module_name":25,"category_name":105,"show_sort_weight":106,"slug":107},16,"Social Media",50,"social-media",{"id":109,"doc_module":22,"doc_module_name":25,"category_name":110,"show_sort_weight":111,"slug":112},17,"Forms",40,"forms",{"id":114,"doc_module":22,"doc_module_name":25,"category_name":115,"show_sort_weight":116,"slug":117},18,"Letters",30,"letters",{"id":119,"doc_module":22,"doc_module_name":25,"category_name":120,"show_sort_weight":121,"slug":122},21,"Paper Templates",5,"papers-templates",{"id":124,"doc_module":22,"doc_module_name":25,"category_name":29,"show_sort_weight":4,"slug":125},158,"general-158",{"code":4,"msg":81,"data":127},{"doc_id":78,"user_id":128,"nickname":42,"user_avatar":129,"doc_module":22,"category_id":124,"category_name":29,"doc_title":10,"doc_description":12,"doc_content":130,"file_id":131,"file_url":132,"file_type":133,"file_size":134,"view_count":33,"is_deleted":4,"is_public":22,"is_downloadable":22,"audit_status":22,"page_count":135,"language":136,"language_code":8,"site_id":7,"html_lang":8,"table_of_contents":137,"faqs":138,"seo_title":139,"seo_description":12,"update_tm":79,"read_time":26},1099523885074,"https://ap-avatar.wpscdn.com/davatar_9964176cb1d06d4a9deccf72a44ae3dc","| TMR-dATP | 100 (10.8) 8.8 (0 .8) | 5.6 (0.8) | 3.4 (0.5) |\n| --- | --- | --- | --- |\n| Cy3-dCTP | 0.3 (0 . 1) 100 (5.3) | 0.42 (0.1) | 0.55 (0.1) |\n| TMR-dGTP | 2.4 (0.4) 0.8 (0.3) | 100 (6.2) | 1.5 (1.0) |\n| Cy5-dUTP | 0.18 (0.2) 0.58 (0.4) | 0.59 (0.5) | 100 (6.0) |\n\n\n| Cy5-ddATP | 100 (16.1) | 0.4 (0.1) | 0.4 (0.1) | 0.5 (0.2) |\n| --- | --- | --- | --- | --- |\n| ROX-ddCTP | 3.5 (0.1) | 100 (15.2) | 6.1 (1.0) | 6.3 (1.1) |\n| FITC-ddGTP | 26.1 (4.8) | 23.2 (6.6) | 100 (6.0) | 19.1 (2.3) |\n| TAMRA-ddUTP | 12.8 (5.9) | 11.0 (2.5) | 8.4 (2.8) | 100 (5.0) |\n\n| PHS1 | 325 | 328 | 49 | 46 | 3 | GC | 35.4 |\n| --- | --- | --- | --- | --- | --- | --- | --- |\n| PHS2 | 295 | 334 | 35 | 32 | 3 | GC | 21.1 |\n| PHS3 | 232 | 234 | 38 | 38 | 0 | GC | 37.3 |\n| PHS4 | 286 | 290 | 25 | 21 | 4 | GC | 11.6 |\n| PHS5 | 274 | 274 | 11 | 10 | 1 | GC | 3.8 |","cbCaipDoTFA1H6Sg","https://ap.wps.com/l/cbCaipDoTFA1H6Sg","pdf",313418,6,"English","# Plasmid DNA Template","[{\"question\":\"What is the purpose of the plasmid DNA template shown in the document?\",\"answer\":\"The plasmid DNA template diagram illustrates the structure of a circular DNA molecule with specific cut sites (EcoRI and NcoI) and a defined region of 2100 bp, serving as a reference for genetic analysis.\"},{\"question\":\"How are different loci differentiated in the polony images?\",\"answer\":\"Different loci are differentiated by fluorescent labels: red indicates Locus A polony, green indicates Locus B polony, and yellow indicates overlapping polonies containing both Locus A and Locus B.\"},{\"question\":\"What is the role of Sequencing By Hybridization (SBE) in this method?\",\"answer\":\"Sequencing By Hybridization (SBE) is used to genotype Single Nucleotide Polymorphisms (SNPs) by detecting specific sequences within the amplified colonies. It is applied sequentially for two different SNPs.\"}]","Plasmid DNA Template | PDF"]