[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"doc-seo-573194-105":3,"doc-detail-573194-en":74,"detail-sidebar-cat-1-en-105":89},{"code":4,"msg":5,"data":6},0,"ok",{"site_id":7,"language":8,"slug":9,"title":10,"keywords":11,"description":12,"schema_data":13,"social_meta":67,"head_meta":69,"extra_data":71,"updated_unix":73},105,"en","phlebotomy-order-of-draw-specimen-collection-guidelines","Phlebotomy Order of Draw - Specimen Collection Guidelines","","This document provides the standardized clinical protocol for the phlebotomy order of draw, essential for ensuring the integrity of blood specimens. It delineates the specific sequence of collection—starting with blood cultures, followed by citrate, serum, heparin, EDTA, and finally glycolytic inhibitors—to prevent cross-contamination of additives between tubes. By strictly adhering to this order, healthcare professionals can mitigate the risk of inaccurate laboratory test results, particularly in chemistry and coagulation studies. The guide serves as a critical reference for medical staff, outlining proper collection procedures using syringes, tube holders, or pre-evacuated tubes to maintain sterile samples and accurate diagnostic outcomes in clinical laboratory settings.",{"@graph":14,"@context":66},[15,34,49],{"@type":16,"itemListElement":17},"BreadcrumbList",[18,23,27,31],{"item":19,"name":20,"@type":21,"position":22},"https://docshare.wps.com","Home","ListItem",1,{"item":24,"name":25,"@type":21,"position":26},"https://docshare.wps.com/template/","Template",2,{"item":28,"name":29,"@type":21,"position":30},"https://docshare.wps.com/template/posters/","Posters",3,{"item":32,"name":10,"@type":21,"position":33},"https://docshare.wps.com/template/phlebotomy-order-of-draw-specimen-collection-guidelines/573194/",4,{"url":32,"name":10,"@type":35,"author":36,"headline":10,"publisher":39,"fileFormat":42,"inLanguage":8,"description":12,"dateModified":43,"datePublished":43,"encodingFormat":42,"isAccessibleForFree":44,"interactionStatistic":45},"DigitalDocument",{"name":37,"@type":38},"Supernature","Person",{"url":19,"name":40,"@type":41},"DocShare","Organization","application/pdf","2026-10-13",true,{"@type":46,"interactionType":47,"userInteractionCount":4},"InteractionCounter",{"@type":48},"ViewAction",{"@type":50,"mainEntity":51},"FAQPage",[52,58,62],{"name":53,"@type":54,"acceptedAnswer":55},"Why must blood be drawn in a specific order?","Question",{"text":56,"@type":57},"Blood must be drawn in a specific order to prevent cross-contamination of additives between collection tubes, which could otherwise adversely affect the accuracy of laboratory test results.","Answer",{"name":59,"@type":54,"acceptedAnswer":60},"What is the first step in the order of draw?",{"text":61,"@type":57},"The first step is the collection of blood cultures to prevent needle contamination when contact is made with non-sterile stoppers from other tubes.",{"name":63,"@type":54,"acceptedAnswer":64},"What is the final step in the sequence?",{"text":65,"@type":57},"The final step is the collection into tubes containing glycolytic inhibitors, such as sodium fluoride or potassium oxalate, to prevent the impact of anticoagulants on red blood cell morphology and chemistry analytes.","https://schema.org",{"og:url":32,"og:type":68,"og:title":10,"og:site_name":40,"og:description":12},"article",{"robots":70,"canonical":32},"index,follow",{"doc_id":72,"site_id":7},573194,1791934455,{"code":4,"msg":75,"data":76},"success",{"doc_id":72,"user_id":77,"nickname":37,"user_avatar":78,"doc_module":22,"category_id":79,"category_name":29,"doc_title":10,"doc_description":12,"doc_content":80,"file_id":81,"file_url":82,"file_type":83,"file_size":84,"view_count":4,"is_deleted":4,"is_public":22,"is_downloadable":22,"audit_status":22,"page_count":22,"language":85,"language_code":8,"site_id":7,"html_lang":8,"table_of_contents":86,"faqs":87,"seo_title":88,"seo_description":12,"update_tm":73,"read_time":4},962093270004,"https://ap-avatar.wpscdn.com/davatar_9964176cb1d06d4a9deccf72a44ae3dc",15,"SPECIMEN COLLECTION: PHLEBOTOMY ORDER OF DRAW  \nBlood samples must be drawn in a specific order to avoid cross-contamination of the sample by additives found in different collection tubes. Phlebotomy order of draw is the same for specimens collected by syringe, tube holder, or into tubes pre-evacuated atthe time of collection. The correct order of draw follows:  \n1. Blood culture tube or bottle  \n2. Sodium citrate tube (eg, blue top)  \n3. Serum tubes, including those with clot activator and gels (eg, red, red-speckled, gold top)  \n4. Heparin tube with or without gel (eg, dark green, light green, speckled green top)  \n5. EDTA tube with or without gel separator (eg, lavender, pearl, pink top)  \n6. Sodium fluoride/potassium oxalate glycolytic inhibitor (eg, gray top)  \n5/20/2021","cbCaip2sRAbTOhRQ","https://ap.wps.com/l/cbCaip2sRAbTOhRQ","pdf",158445,"English","# Phlebotomy Order of Draw Procedures\n## Standardized Collection Sequence","[{\"question\":\"Why must blood be drawn in a specific order?\",\"answer\":\"Blood must be drawn in a specific order to prevent cross-contamination of additives between collection tubes, which could otherwise adversely affect the accuracy of laboratory test results.\"},{\"question\":\"What is the first step in the order of draw?\",\"answer\":\"The first step is the collection of blood cultures to prevent needle contamination when contact is made with non-sterile stoppers from other tubes.\"},{\"question\":\"What is the final step in the sequence?\",\"answer\":\"The final step is the collection into tubes containing glycolytic inhibitors, such as sodium fluoride or potassium oxalate, to prevent the impact of anticoagulants on red blood cell morphology and chemistry analytes.\"}]","Phlebotomy Order of Draw - Specimen Collection Guidelines | PDF",{"code":4,"msg":75,"data":90},[91,96,101,106,109,114,119,124,129],{"id":92,"doc_module":22,"doc_module_name":25,"category_name":93,"show_sort_weight":94,"slug":95},11,"Presentations",90,"presentations",{"id":97,"doc_module":22,"doc_module_name":25,"category_name":98,"show_sort_weight":99,"slug":100},12,"Resumes",80,"resumes",{"id":102,"doc_module":22,"doc_module_name":25,"category_name":103,"show_sort_weight":104,"slug":105},14,"Invoices",70,"invoices",{"id":79,"doc_module":22,"doc_module_name":25,"category_name":29,"show_sort_weight":107,"slug":108},60,"posters",{"id":110,"doc_module":22,"doc_module_name":25,"category_name":111,"show_sort_weight":112,"slug":113},16,"Social Media",50,"social-media",{"id":115,"doc_module":22,"doc_module_name":25,"category_name":116,"show_sort_weight":117,"slug":118},17,"Forms",40,"forms",{"id":120,"doc_module":22,"doc_module_name":25,"category_name":121,"show_sort_weight":122,"slug":123},18,"Letters",30,"letters",{"id":125,"doc_module":22,"doc_module_name":25,"category_name":126,"show_sort_weight":127,"slug":128},21,"Paper Templates",5,"papers-templates",{"id":130,"doc_module":22,"doc_module_name":25,"category_name":131,"show_sort_weight":4,"slug":132},158,"General","general-158"]