[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"detail-sidebar-cat-1-en-105":3,"doc-seo-571729-105":53,"doc-detail-571729-en":120},{"code":4,"msg":5,"data":6},0,"success",[7,14,19,24,29,34,39,44,49],{"id":8,"doc_module":9,"doc_module_name":10,"category_name":11,"show_sort_weight":12,"slug":13},11,1,"Template","Presentations",90,"presentations",{"id":15,"doc_module":9,"doc_module_name":10,"category_name":16,"show_sort_weight":17,"slug":18},12,"Resumes",80,"resumes",{"id":20,"doc_module":9,"doc_module_name":10,"category_name":21,"show_sort_weight":22,"slug":23},14,"Invoices",70,"invoices",{"id":25,"doc_module":9,"doc_module_name":10,"category_name":26,"show_sort_weight":27,"slug":28},15,"Posters",60,"posters",{"id":30,"doc_module":9,"doc_module_name":10,"category_name":31,"show_sort_weight":32,"slug":33},16,"Social Media",50,"social-media",{"id":35,"doc_module":9,"doc_module_name":10,"category_name":36,"show_sort_weight":37,"slug":38},17,"Forms",40,"forms",{"id":40,"doc_module":9,"doc_module_name":10,"category_name":41,"show_sort_weight":42,"slug":43},18,"Letters",30,"letters",{"id":45,"doc_module":9,"doc_module_name":10,"category_name":46,"show_sort_weight":47,"slug":48},21,"Paper Templates",5,"papers-templates",{"id":50,"doc_module":9,"doc_module_name":10,"category_name":51,"show_sort_weight":4,"slug":52},158,"General","general-158",{"code":4,"msg":54,"data":55},"ok",{"site_id":56,"language":57,"slug":58,"title":59,"keywords":60,"description":61,"schema_data":62,"social_meta":113,"head_meta":115,"extra_data":117,"updated_unix":119},105,"en","order-of-draw-and-order-of-transfer-for-blood-collection-tubes","Order of Draw and Order of Transfer for Blood Collection Tubes","","Guidance for filling blood collection tubes in a specific order to reduce contamination of sterile specimens and prevent test result errors from cross contamination by tube additives. Covers use with evacuated tube systems and syringe-to-evacuated-tube transfer, including when skin puncture and microcollection tubes require a different referenced order. Provides ordered selection of tube types, inversion counts, and special instructions for blood cultures and Paxgene tubes. Includes trace element collection rules and key references.",{"@graph":63,"@context":112},[64,80,95],{"@type":65,"itemListElement":66},"BreadcrumbList",[67,71,74,77],{"item":68,"name":69,"@type":70,"position":9},"https://docshare.wps.com","Home","ListItem",{"item":72,"name":10,"@type":70,"position":73},"https://docshare.wps.com/template/",2,{"item":75,"name":36,"@type":70,"position":76},"https://docshare.wps.com/template/forms/",3,{"item":78,"name":59,"@type":70,"position":79},"https://docshare.wps.com/template/order-of-draw-and-order-of-transfer-for-blood-collection-tubes/571729/",4,{"url":78,"name":59,"@type":81,"author":82,"headline":59,"publisher":85,"fileFormat":88,"inLanguage":57,"description":61,"dateModified":89,"datePublished":89,"encodingFormat":88,"isAccessibleForFree":90,"interactionStatistic":91},"DigitalDocument",{"name":83,"@type":84},"Sarah ","Person",{"url":68,"name":86,"@type":87},"DocShare","Organization","application/pdf","2026-10-13",true,{"@type":92,"interactionType":93,"userInteractionCount":4},"InteractionCounter",{"@type":94},"ViewAction",{"@type":96,"mainEntity":97},"FAQPage",[98,104,108],{"name":99,"@type":100,"acceptedAnswer":101},"Why must blood collection tubes be filled in a specific order of draw?","Question",{"text":102,"@type":103},"To minimize contamination of sterile specimens and avoid potential test result errors caused by cross contamination from tube additives.","Answer",{"name":105,"@type":100,"acceptedAnswer":106},"What is the correct order for blood culture bottles?",{"text":107,"@type":103},"The aerobic bottle should be collected before the anaerobic bottle, with gentle inversion to mix.",{"name":109,"@type":100,"acceptedAnswer":110},"How should trace elements be collected?",{"text":111,"@type":103},"Use a separate venipuncture for trace elements, and collect the non-additive trace element tube first; other tubes may follow with restrictions.","https://schema.org",{"og:url":78,"og:type":114,"og:title":59,"og:site_name":86,"og:description":61},"article",{"robots":116,"canonical":78},"index,follow",{"doc_id":118,"site_id":56},571729,1791923417,{"code":4,"msg":5,"data":121},{"doc_id":118,"user_id":122,"nickname":83,"user_avatar":123,"doc_module":9,"category_id":35,"category_name":36,"doc_title":59,"doc_description":61,"doc_content":124,"file_id":125,"file_url":126,"file_type":127,"file_size":128,"view_count":4,"is_deleted":4,"is_public":9,"is_downloadable":9,"audit_status":9,"page_count":9,"language":129,"language_code":57,"site_id":56,"html_lang":57,"table_of_contents":130,"faqs":131,"seo_title":132,"seo_description":61,"update_tm":119,"read_time":4},962085320529,"https://ap-avatar.wpscdn.com/davatar_9964176cb1d06d4a9deccf72a44ae3dc","Blood collection tubes must be filled in a specific order to minimize contamination of sterile specimens and avoid possible test result error due to cross contamination from tube additives. Refer to the APL Test Directory for specific information on the collection and handling of laboratory tests.  \nUse this document for evacuated tube systems and when transferring syringe collected blood into evacuated tube systems. For skin puncture and microcollection tubes refer to PA03-010 Skin Puncture Order of Collection.  \n\n| Order | Tube or Container |  |  |  |  |  |  | Invert | Notes |\n| --- | --- | --- | --- | --- | --- | --- | --- | --- | --- |\n| \u003Cbr>| Blood culture bottles, both adult and pediatric, e.g.: |  |  |  |  |  |  | Invert gently to mix | The aerobic bottle should be\u003Cbr>collected before the anaerobic bottle. |\n|  | |  |  |  | | | |  |  |\n|  | Pale yellow SPS tubes with clear label\u003Cbr>|  |  |  |  |  |  | 8 to 10 times | Tube used for Mycobacteria (AFB) blood culture. |\n|  | Sodium citrate tubes, e.g. light blue, black Excyte\u003Cbr>|  |  |  |  |  |  | 3 to 4 times |  |\n|  | ACDA tubes, e.g. yellow with yellow band trisodium citrate\u003Cbr>|  |  |  |  |  |  | 8 to 10 times |  |\n|  | Serum tubes, including those with clot activators and gel, [e.g. red](e.g. red), gold, orange RST\u003Cbr>|  |  |  |  |  |  | 8 to 10 times |  |\n|  | Heparin tubes, including those with gel, [e.g. green](e.g. green), mint, lime |  |  |  |  |  |  | 8 to 10 times |  |\n|  | | | |  |  |  |  |  |  |\n|  | EDTA tubes, e.g. lavender, pink, clear PPT, catecholamine tubes |  |  |  |  |  |  | 8 to 10 times |  |\n|  | | |  | |  |  |  |  |  |\n|  | Streck Cell-Free BCT tubes, black and brown\u003Cbr>|  |  |  |  |  |  | 8 to 10 times |  |\n|  | Sodium fluoride/potassium oxalate glycolytic inhibitor tubes, e.g. grey\u003Cbr>|  |  |  |  |  |  | 8 to 10 times |  |\n|  | Other additive tubes |  |  |  |  |  |  | 8 to 10 times | Refer to manufacturer’s instructions for order of draw for other additive tubes. If no instructions are provided, collect after other tubes. |\n| Always\u003Cbr>last | Paxgene tubes\u003Cbr>|  |  |  |  |  |  | 8 to 10 times |  |\n\nNOTE: When an anticoagulated syringe collection (example PICO syringe) is requested in addition to evacuated tubes, collect the evacuated tubes first , then a discard tube is required before collecting the anticoagulated syringe when the order of draw cannot be maintained.  \nTrace Element Testing  \n􀁸 A separate venipuncture is recommended for trace elements to assure there is no trace metal contamination on the needle from puncturing other tube stoppers.  \n􀁸 The non-additive trace element tube is collected first.  \n\n| Trace elements non additive tube ,\u003Cbr>royal blue with no additive  | 8 to 10 times | When blood cultures are ordered a separate venipuncture fortrace metals is always required.\u003Cbr>Other tubes may be collected after trace element tubes with restrictions. Non additive trace element tube will always be collected first.\u003Cbr>􀁸 When a trace element tube royal blue with sodium heparin tube is required, other heparin tubes can be collected after this tube, and also any subsequent tubes types as per the order of draw. |\n| --- | --- | --- |\n| Trace elements additive tube , royal\u003Cbr>blue with sodium heparin  | 8 to 10 times |  |\n\nREFERENCES  \n1. CLSI. Collection of Diagnostic Venous Blood Specimens , 7th ed. CLSI standard GP41 . Wayne, Pennsylvania: Clinical and Laboratory Standards Institute, 2017.  \n2. Becton, Dickinson and Company, BD Vacutainer Order of Draw for Multiple Tube Collections, document VS5729-6. Franklin Lakes, NJ, January 2010.  \n3. National Center for Competency Testing, Phlebotomy Order of Draw, Rev 7.0 August 2013Becton, Dickinson and Company. BD Vacutainer Evacuated Blood Collection System. Package Insert. VDP40161-WEB-09. 06/2019 (and other package inserts for blood collection tubes, as appropriate) .","cbCainhBeLRQlfbt","https://ap.wps.com/l/cbCainhBeLRQlfbt","pdf",205241,"English","# Order of draw for evacuated tubes\n## Blood culture bottles\n## SPS, citrate, ACDA\n## Serum, heparin, EDTA\n## Cell-free BCT and fluoride/oxalate tubes\n## Other additive tubes and Paxgene tubes\n## Anticoagulated syringe collections (PICO)\n# Trace element testing\n## Non-additive trace element tube first\n## Additive trace element tube","[{\"question\":\"Why must blood collection tubes be filled in a specific order of draw?\",\"answer\":\"To minimize contamination of sterile specimens and avoid potential test result errors caused by cross contamination from tube additives.\"},{\"question\":\"What is the correct order for blood culture bottles?\",\"answer\":\"The aerobic bottle should be collected before the anaerobic bottle, with gentle inversion to mix.\"},{\"question\":\"How should trace elements be collected?\",\"answer\":\"Use a separate venipuncture for trace elements, and collect the non-additive trace element tube first; other tubes may follow with restrictions.\"}]","Order of Draw and Order of Transfer for Blood Collection Tubes | PDF"]