[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"detail-sidebar-cat-1-en-105":3,"doc-seo-233958-105":53,"doc-detail-233958-en":127},{"code":4,"msg":5,"data":6},0,"success",[7,14,19,24,29,34,39,44,49],{"id":8,"doc_module":9,"doc_module_name":10,"category_name":11,"show_sort_weight":12,"slug":13},11,1,"Template","Presentations",90,"presentations",{"id":15,"doc_module":9,"doc_module_name":10,"category_name":16,"show_sort_weight":17,"slug":18},12,"Resumes",80,"resumes",{"id":20,"doc_module":9,"doc_module_name":10,"category_name":21,"show_sort_weight":22,"slug":23},14,"Invoices",70,"invoices",{"id":25,"doc_module":9,"doc_module_name":10,"category_name":26,"show_sort_weight":27,"slug":28},15,"Posters",60,"posters",{"id":30,"doc_module":9,"doc_module_name":10,"category_name":31,"show_sort_weight":32,"slug":33},16,"Social Media",50,"social-media",{"id":35,"doc_module":9,"doc_module_name":10,"category_name":36,"show_sort_weight":37,"slug":38},17,"Forms",40,"forms",{"id":40,"doc_module":9,"doc_module_name":10,"category_name":41,"show_sort_weight":42,"slug":43},18,"Letters",30,"letters",{"id":45,"doc_module":9,"doc_module_name":10,"category_name":46,"show_sort_weight":47,"slug":48},21,"Paper Templates",5,"papers-templates",{"id":50,"doc_module":9,"doc_module_name":10,"category_name":51,"show_sort_weight":4,"slug":52},158,"General","general-158",{"code":4,"msg":54,"data":55},"ok",{"site_id":56,"language":57,"slug":58,"title":59,"keywords":60,"description":61,"schema_data":62,"social_meta":120,"head_meta":122,"extra_data":124,"updated_unix":126},105,"en","motivation-letter-ayush-deep-structural-biology-research-goals-eth-zurich","Motivation Letter - Ayush Deep - Structural Biology Research Goals - ETH Zurich","","Motivation letter combining personal academic curiosity with hands-on structural biology training. The author describes a routine of identifying macromolecular complexes, retrieving structures from the Protein Data Bank, and analyzing them in PyMOL, which led to a research focus on structural biology. It highlights laboratory experience in protein overexpression and purification, GTPase assay standardization, and cryo-EM data processing with tools including cryoSPARC, Chimera/ChimeraX, Coot, and PHENIX, plus grid preparation. The letter outlines an intention to pursue ETH Zurich master’s study aimed at resolving nutrient-starvation and mTOR inhibition mechanisms underlying ribophagy.",{"@graph":63,"@context":119},[64,80,102],{"@type":65,"itemListElement":66},"BreadcrumbList",[67,71,74,77],{"item":68,"name":69,"@type":70,"position":9},"https://docshare.wps.com","Home","ListItem",{"item":72,"name":10,"@type":70,"position":73},"https://docshare.wps.com/template/",2,{"item":75,"name":41,"@type":70,"position":76},"https://docshare.wps.com/template/letters/",3,{"item":78,"name":59,"@type":70,"position":79},"https://docshare.wps.com/template/motivation-letter-ayush-deep-structural-biology-research-goals-eth-zurich/233958/",4,{"url":78,"name":59,"@type":81,"image":82,"author":87,"headline":59,"publisher":90,"fileFormat":93,"inLanguage":57,"description":61,"dateModified":94,"datePublished":95,"encodingFormat":93,"isAccessibleForFree":96,"interactionStatistic":97},"DigitalDocument",{"url":83,"@type":84,"width":85,"height":86},"https://docshare.wps.com/thumbnails/motivation-letter-ayush-deep-structural-biology-research-goals-eth-zurich/233958.png","ImageObject",442,249,{"name":88,"@type":89},"Quinn","Person",{"url":68,"name":91,"@type":92},"DocShare","Organization","application/pdf","2026-10-19","2026-09-10",true,{"@type":98,"interactionType":99,"userInteractionCount":101},"InteractionCounter",{"@type":100},"ViewAction",13,{"@type":103,"mainEntity":104},"FAQPage",[105,111,115],{"name":106,"@type":107,"acceptedAnswer":108},"What prompted the author to pursue structural biology research?","Question",{"text":109,"@type":110},"Persistent curiosity about how simplified molecular drawings relate to real macromolecular structures led the author to analyze complexes via Protein Data Bank and PyMOL. Discovering that important proteins like BRCA2 still lacked complete structures further motivated this direction.","Answer",{"name":112,"@type":107,"acceptedAnswer":113},"What structural biology skills and lab work does the author highlight?",{"text":114,"@type":110},"The author emphasizes protein overexpression and purification, protein affinity purification methods, basic molecular biology techniques, and research assistant experience. It also covers cryo-EM data processing using cryoSPARC, visualization with Chimera/ChimeraX, and model building/refinement with Coot and PHENIX, along with grid preparation.",{"name":116,"@type":107,"acceptedAnswer":117},"What research goal is described for the ETH Zurich master’s program?",{"text":118,"@type":110},"The primary goal is to determine the structure of the 60S pre-ribophagy complex (60S-NUFIP1-ZNHIT3) involved in nutrient-limiting conditions and mTOR inhibition-driven ribophagy.","https://schema.org",{"og:url":78,"og:type":121,"og:title":59,"og:site_name":91,"og:description":61},"article",{"robots":123,"canonical":78},"index,follow",{"doc_id":125,"site_id":56},233958,1790661206,{"code":4,"msg":5,"data":128},{"doc_id":125,"user_id":129,"nickname":88,"user_avatar":130,"doc_module":9,"category_id":40,"category_name":41,"doc_title":59,"doc_description":61,"doc_content":131,"file_id":132,"file_url":133,"file_type":134,"file_size":135,"view_count":101,"is_deleted":4,"is_public":9,"is_downloadable":9,"audit_status":9,"page_count":47,"language":136,"language_code":57,"site_id":56,"html_lang":57,"table_of_contents":137,"faqs":138,"seo_title":139,"seo_description":61,"update_tm":140,"read_time":73},962075114765,"https://ap-avatar.wpscdn.com/davatar_a8503ba1806abce46bf441b54a3ca4cd","Motivation Letter  \nAyush Deep  \nOne of my most impactful experiences during college occurred during my third year molecular biology course. Almost every lecture, I was enthralled by the different macromolecular complexes that were the focus of the class. I was primarily intrigued by the simple shapes the professor had used as representations in his powerpoints. “How accurate could these simple drawings be to the actual structures of these complexes?” I would think. This curiosity eventually resulted in the development of my daily routine; I would take note of complexes that I found particularly interesting, find their structures in the Protein Data Bank, and then examine them in PyMOL.  \nI was fascinated by how diverse many of these macromolecules looked, both in size and shape. Something even more fascinating to me was how their structure affected their function. Once in a while, I would end up on seemingly endless searches for the structure of a protein or macromolecular complex. My inability to find a structure would lead me to conclude that this biomolecule had yet to be visualized. What surprised me most was that many of these mystery biomolecules were involved in pathways critical to human health and disease. A particular example that stood out to me was the DNA damage repair protein, BRCA2, for which I found a number of partial structures, but no complete structure. As this protein played an important role in tumor suppression, it was striking to me that it’s full structure had yet to be solved. Realizing that there are many important biological complexes, such as BRCA2, that remain to be visualized, I was motivated to pursue research in the field of structural biology. I was fortunate enough to have the opportunity to conduct my bachelor’s thesis research in a structural biology lab, learning a great deal along the way. Now I would like to continue my education to explore the topic further in depth. Hence, Iam applying to the Master’s program in Biology at ETH Zurich, with the goal of conducting structural biology research to gain a better understanding of the molecular world.  \nThree years of experience working in the Agrawal lab has prepared me for challenges faced in the field of structural biology. During my first two years in the lab I was tasked with the overexpression and purification of a translational factor in a bacterial overexpression system. Over the course of this project I learned a great deal of protein biochemistry, primarily protein affinity purification methods, along with basic molecular biology lab techniques, such as culturing bacteria, western blotting, SDS-PAGE, and PCR. Since graduating, I have been working full time as a research assistant in the same lab and I have had more opportunities to explore a diverse array of projects. In one of the projects, I standardized a GTPase assay to measure the differences in susceptibility of different types of ribosomes to a variety of antibiotics. I also analyzed these structures and was able to determine the molecular basis of susceptibility or resistance. In another project I processed cryo-EM data of the small ribosomal subunit from M. smegmatis grown in nutrient limiting conditions. I interpreted the different classes of density maps obtained from this dataset and identified the presence of multiple translational factors bound to the subunit. I have learned a number of key computational skills important for structural biology through each project. These include scripting in the Unix command line, cryo-EM data processing with cryoSPARC, using Chimera and ChimeraX for the visualization of maps and PDBs, and using Coot and  \nPHENIX for model building and refinement. In addition, I have also been learning how to vitrify and prepare grids for data collection.  \nThough there are many excellent structural biology research groups present at ETH Zurich, I am keen to work in the lab of Dr. Nenad Ban. My interest stems from the lab’s work on the structure determ","cbCaicoSfsyZW1F1","https://ap.wps.com/l/cbCaicoSfsyZW1F1","pdf",68627,"English","# Motivation Letter\n## Background\n## Objectives and goals","[{\"question\":\"What prompted the author to pursue structural biology research?\",\"answer\":\"Persistent curiosity about how simplified molecular drawings relate to real macromolecular structures led the author to analyze complexes via Protein Data Bank and PyMOL. Discovering that important proteins like BRCA2 still lacked complete structures further motivated this direction.\"},{\"question\":\"What structural biology skills and lab work does the author highlight?\",\"answer\":\"The author emphasizes protein overexpression and purification, protein affinity purification methods, basic molecular biology techniques, and research assistant experience. It also covers cryo-EM data processing using cryoSPARC, visualization with Chimera/ChimeraX, and model building/refinement with Coot and PHENIX, along with grid preparation.\"},{\"question\":\"What research goal is described for the ETH Zurich master’s program?\",\"answer\":\"The primary goal is to determine the structure of the 60S pre-ribophagy complex (60S-NUFIP1-ZNHIT3) involved in nutrient-limiting conditions and mTOR inhibition-driven ribophagy.\"}]","Motivation Letter - Ayush Deep - Structural Biology Research Goals - ETH Zurich | PDF",1789082167]