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Provides step-by-step cleaning program workflows (Clean decontamination, Rinse between plates, and Flush for clogs and monthly maintenance) with timing and reagent details. Includes PMT calibration with barcode reading, bead mixing and dilution, and analysis pass checks. Describes acquisition workspace setup, rack selection, sample and flow-rate configuration, gating and plot layout, plate acquisition control, and data retrieval to a USB drive, finishing with shutdown procedures.",{"@graph":63,"@context":118},[64,80,101],{"@type":65,"itemListElement":66},"BreadcrumbList",[67,71,74,77],{"item":68,"name":69,"@type":70,"position":9},"https://docshare.wps.com","Home","ListItem",{"item":72,"name":10,"@type":70,"position":73},"https://docshare.wps.com/template/",2,{"item":75,"name":11,"@type":70,"position":76},"https://docshare.wps.com/template/presentations/",3,{"item":78,"name":59,"@type":70,"position":79},"https://docshare.wps.com/template/macsquant-analyzer-quick-guide-cleaning-and-acquisition-setup/172769/",4,{"url":78,"name":59,"@type":81,"image":82,"author":87,"headline":59,"publisher":90,"fileFormat":93,"inLanguage":57,"description":61,"dateModified":94,"datePublished":95,"encodingFormat":93,"isAccessibleForFree":96,"interactionStatistic":97},"DigitalDocument",{"url":83,"@type":84,"width":85,"height":86},"https://docshare.wps.com/thumbnails/macsquant-analyzer-quick-guide-cleaning-and-acquisition-setup/172769.png","ImageObject",442,249,{"name":88,"@type":89},"Nguyễn Văn Học","Person",{"url":68,"name":91,"@type":92},"DocShare","Organization","application/vnd.openxmlformats-officedocument.wordprocessingml.document","2026-10-23","2026-09-01",true,{"@type":98,"interactionType":99,"userInteractionCount":15},"InteractionCounter",{"@type":100},"ViewAction",{"@type":102,"mainEntity":103},"FAQPage",[104,110,114],{"name":105,"@type":106,"acceptedAnswer":107},"How should the optical bench be prepared before cleaning and acquisition?","Question",{"text":108,"@type":109},"Optical bench warm-up requires about 30 minutes. Complete the Start Up steps 1–5 to initialize warm-up and begin cleaning.","Answer",{"name":111,"@type":106,"acceptedAnswer":112},"Which cleaning program should be used at startup, between plates, and for monthly maintenance?",{"text":113,"@type":109},"Use “Clean” for start-up decontamination, “Rinse” between plates, and “Flush” for clearing clogs and monthly maintenance.",{"name":115,"@type":106,"acceptedAnswer":116},"How are acquisition settings configured for a plate workflow?",{"text":117,"@type":109},"Create or open a workspace, select the appropriate rack in the Experiment tab, assign Sample IDs per well or well groups, set Flow Rate (Low 25 µL/min, Medium 50 µL/min, High 100 µL/min), enable “Mix Sample,” and configure Events if you want the instrument to stop after a target number.","https://schema.org",{"og:url":78,"og:type":120,"og:title":59,"og:site_name":91,"og:description":61},"article",{"robots":122,"canonical":78},"index,follow",{"doc_id":124,"site_id":56},172769,1791425327,{"code":4,"msg":5,"data":127},{"doc_id":124,"user_id":128,"nickname":88,"user_avatar":129,"doc_module":9,"category_id":8,"category_name":11,"doc_title":59,"doc_description":61,"doc_content":130,"file_id":131,"file_url":132,"file_type":133,"file_size":134,"view_count":15,"is_deleted":4,"is_public":9,"is_downloadable":9,"audit_status":9,"page_count":73,"language":135,"language_code":57,"site_id":56,"html_lang":57,"table_of_contents":136,"faqs":137,"seo_title":138,"seo_description":61,"update_tm":139,"read_time":9},1374402739827,"https://ap-avatar.wpscdn.com/avatar/14000c97e7351f1a627?x-image-process=image/resize,m_fixed,w_180,h_180&k=1787885694763230660","MACSQuant Analyzer Quick Guide\nNOTE: Optical bench requires a 30 minute warm-up. Please complete “Start Up” steps 1-5 to initialize warm-up and begin cleaning.\nStart Up\nTurn on Power switch on Right side of instrument.\nTouch the monitor to wake up instrument.\nLog in under your PI.\nTo initialize warm-up, click the\nicon in the upper right corner and select “Acquisition Mode”.\nRun “Clean” Program (#1 below).\nCleaning Programs\nClean: Decontamination at start up.\nRight-click, or press and HOLD the ( icon at the bottom of the screen and select “Clean.”\nThis mode cleans with 10% bleach and takes 10 minutes.\nRinse: Between plates.\nClick the status bar ( icon at the bottom of the screen.\nThis mode flushes aggregates and takes about 5 minutes.\nFlush: Clearing clogs and monthly maintenance.\nRight-click, or press and HOLD the ( icon at the bottom of the screen and select “Flush.”\nThis mode incubates the flow cell and takes 16 minutes.\nPMT Calibration\nRetrieve the MACSQuant Calibration Beads from the core refrigerator and vortex.\nClick the barcode\nicon and present the vial barcode to the reader when it begins to flash.\nIt has read the barcode when the reader flashes green.\nFollow the on-screen prompts to proceed.\nPlace a 5mL tube in the single tube holder.\nPlace one drop of beads into the tube and press “OK.”\nMACSQuant will automatically mix and dilute beads, open template and begin analysis.\nOnce Analysis is complete, make sure all parameters have “Passed.”\nTurn off the orange highlighted A icon at the top of the screen to exit analysis mode.\nAcquisition Settings\nGo to File > New Workspace (or File > Open to open existing workspace).\nIn the Experiment Tab, select appropriate rack (ie. Chill 96-Well Rack).\nA rack template should pop up in the middle of the screen. Click on the wells you want to analyze.\nWells Highlighted with an orange ring will be programmed using the Experiment tab.\nSet your Sample ID for each well, row or column by highlighting the desired wells.\nSelect all wells and set Flow Rate.\nLow = 25uL/min for high cell densities or sensitive measurements.\nMedium = 50uL/min.\nHigh = 100uL/min for low densities and quick analysis.\nWell A1 will be used to set parameters, it is wise to set this well to run in LOW.\nAlways check “Mix Sample”\nMode is always standard\nUptake Volume is the amount to be analyzed (cannot be greater or equal to Sample Volume).\nSample Volume is the total volume in the well (if this is overestimated, Instrument may take up air during mixing).\nAnnotations Tab: Set labels of Antibodies and fluorophores.\nSettings Tab: Check “Events” and set number desired only if you want the instrument to stop and move on to the next sample once it has reached this number of events. (10.000=10,000)\nDefine Layout\nOn the top of the screen, click on the icon that looks like a dot plot.\nSelect a layout suitable for your analysis.\nChange axis on plots and set a FSC/SSC “P1” gate.\nSet other plots to P1 by selecting “Live/P1” from the pull down at the top of the plot.\nPlate Acquisition\nOnce you have your desired wells configured and your plots displayed, click on the\nicon in the bottom right hand corner to begin plate acquisition.\nNOTE: If you need to stop the acquisition, press the\nicon in the bottom right hand corner. In order to resume this plate, you may want to delete wells from the template that have already been run or the instrument will attempt to reacquire them.\nDuring the first well, your unstained sample, click on the Channels tab and adjust FSC/SSC until your cells are centered in the dot plot and within P1.\nThen adjust other applicable colored parameters so that your cells are within the first grid decade, below 1e0.\nOnce you have the parameters set, press the “Clear” button on the bottom center of the screen to refresh the data file.\nContinue to run the remainder of the plate.\nData Retrieval\nPlace a flash drive into the USB hub to the left of the MACSQuant.\nSelect File > Copy\nIn the window that pops up, select “Data” from","cbCais7EHPT4idyj","https://ap.wps.com/l/cbCais7EHPT4idyj","docx",31028,"English","# Start Up\n## Cleaning Programs\n## PMT Calibration\n## Acquisition Settings\n## Define Layout\n## Plate Acquisition\n## Data Retrieval\n## Shut Down","[{\"question\":\"How should the optical bench be prepared before cleaning and acquisition?\",\"answer\":\"Optical bench warm-up requires about 30 minutes. Complete the Start Up steps 1–5 to initialize warm-up and begin cleaning.\"},{\"question\":\"Which cleaning program should be used at startup, between plates, and for monthly maintenance?\",\"answer\":\"Use “Clean” for start-up decontamination, “Rinse” between plates, and “Flush” for clearing clogs and monthly maintenance.\"},{\"question\":\"How are acquisition settings configured for a plate workflow?\",\"answer\":\"Create or open a workspace, select the appropriate rack in the Experiment tab, assign Sample IDs per well or well groups, set Flow Rate (Low 25 µL/min, Medium 50 µL/min, High 100 µL/min), enable “Mix Sample,” and configure Events if you want the instrument to stop after a target number.\"}]","MACSQuant Analyzer Quick Guide - Cleaning and Acquisition Setup | DOCX",1788296367]