[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"detail-sidebar-cat-1-en-105":3,"doc-seo-177602-105":53,"doc-detail-177602-en":126},{"code":4,"msg":5,"data":6},0,"success",[7,14,19,24,29,34,39,44,49],{"id":8,"doc_module":9,"doc_module_name":10,"category_name":11,"show_sort_weight":12,"slug":13},11,1,"Template","Presentations",90,"presentations",{"id":15,"doc_module":9,"doc_module_name":10,"category_name":16,"show_sort_weight":17,"slug":18},12,"Resumes",80,"resumes",{"id":20,"doc_module":9,"doc_module_name":10,"category_name":21,"show_sort_weight":22,"slug":23},14,"Invoices",70,"invoices",{"id":25,"doc_module":9,"doc_module_name":10,"category_name":26,"show_sort_weight":27,"slug":28},15,"Posters",60,"posters",{"id":30,"doc_module":9,"doc_module_name":10,"category_name":31,"show_sort_weight":32,"slug":33},16,"Social Media",50,"social-media",{"id":35,"doc_module":9,"doc_module_name":10,"category_name":36,"show_sort_weight":37,"slug":38},17,"Forms",40,"forms",{"id":40,"doc_module":9,"doc_module_name":10,"category_name":41,"show_sort_weight":42,"slug":43},18,"Letters",30,"letters",{"id":45,"doc_module":9,"doc_module_name":10,"category_name":46,"show_sort_weight":47,"slug":48},21,"Paper Templates",5,"papers-templates",{"id":50,"doc_module":9,"doc_module_name":10,"category_name":51,"show_sort_weight":4,"slug":52},158,"General","general-158",{"code":4,"msg":54,"data":55},"ok",{"site_id":56,"language":57,"slug":58,"title":59,"keywords":60,"description":61,"schema_data":62,"social_meta":119,"head_meta":121,"extra_data":123,"updated_unix":125},105,"en","group-b-streptococcus-gbs-colonisation-dynamics-and-cps-specific-antibody-stability","Group B Streptococcus (GBS) colonisation dynamics and CPS-specific antibody stability","","Group B Streptococcus (GBS) drives adverse pregnancy outcomes through invasive neonatal infection, making immune response dynamics clinically important. This longitudinal study measured rectovaginal colonisation and CPS-specific immune markers across a 12-week follow-up in non-pregnant UK women, using PCR serotyping from swabs and Luminex/ELISA assays for serum, vaginal, and nasal IgG/IgA. GBS carriage fluctuated, while CPS-specific antibody levels remained stable. Serum CPS-specific antibodies were higher with colonisation than without, and correlations were observed across compartments.",{"@graph":63,"@context":118},[64,80,101],{"@type":65,"itemListElement":66},"BreadcrumbList",[67,71,74,77],{"item":68,"name":69,"@type":70,"position":9},"https://docshare.wps.com","Home","ListItem",{"item":72,"name":10,"@type":70,"position":73},"https://docshare.wps.com/template/",2,{"item":75,"name":51,"@type":70,"position":76},"https://docshare.wps.com/template/general/",3,{"item":78,"name":59,"@type":70,"position":79},"https://docshare.wps.com/template/group-b-streptococcus-gbs-colonisation-dynamics-and-cps-specific-antibody-stability/177602/",4,{"url":78,"name":59,"@type":81,"image":82,"author":87,"headline":59,"publisher":90,"fileFormat":93,"inLanguage":57,"description":61,"dateModified":94,"datePublished":95,"encodingFormat":93,"isAccessibleForFree":96,"interactionStatistic":97},"DigitalDocument",{"url":83,"@type":84,"width":85,"height":86},"https://docshare.wps.com/thumbnails/group-b-streptococcus-gbs-colonisation-dynamics-and-cps-specific-antibody-stability/177602.png","ImageObject",442,249,{"name":88,"@type":89},"Ophelia","Person",{"url":68,"name":91,"@type":92},"DocShare","Organization","application/vnd.openxmlformats-officedocument.wordprocessingml.document","2026-09-30","2026-09-02",true,{"@type":98,"interactionType":99,"userInteractionCount":47},"InteractionCounter",{"@type":100},"ViewAction",{"@type":102,"mainEntity":103},"FAQPage",[104,110,114],{"name":105,"@type":106,"acceptedAnswer":107},"What does the study examine about Group B Streptococcus during follow-up?","Question",{"text":108,"@type":109},"It examines longitudinal variation in GBS rectovaginal colonisation and CPS-specific antibody levels in serum and vaginal compartments over a 12-week period.","Answer",{"name":111,"@type":106,"acceptedAnswer":112},"How were participants and samples collected and analyzed?",{"text":113,"@type":109},"Non-pregnant women were sampled every two weeks for 12 weeks. GBS isolates from recto-vaginal swabs were PCR serotyped, and CPS-specific IgG/IgA and total IgG/A were measured using Luminex and ELISA.",{"name":115,"@type":106,"acceptedAnswer":116},"What is the main relationship between GBS colonisation dynamics and CPS-specific antibody levels?",{"text":117,"@type":109},"Although detectable GBS carriage fluctuated over time, CPS-specific antibody levels in serum, vaginal tissue, and nasal measures remained constant.","https://schema.org",{"og:url":78,"og:type":120,"og:title":59,"og:site_name":91,"og:description":61},"article",{"robots":122,"canonical":78},"index,follow",{"doc_id":124,"site_id":56},177602,1790228729,{"code":4,"msg":5,"data":127},{"doc_id":124,"user_id":128,"nickname":88,"user_avatar":129,"doc_module":9,"category_id":50,"category_name":51,"doc_title":59,"doc_description":61,"doc_content":130,"file_id":131,"file_url":132,"file_type":133,"file_size":134,"view_count":47,"is_deleted":4,"is_public":9,"is_downloadable":9,"audit_status":9,"page_count":135,"language":136,"language_code":57,"site_id":56,"html_lang":57,"table_of_contents":137,"faqs":138,"seo_title":139,"seo_description":61,"update_tm":140,"read_time":15},7971461741311,"https://ap-avatar.wpscdn.com/avatar/74000253aff267980c6?x-image-process=image/resize,m_fixed,w_180,h_180&k=1779345379180704826","Group B Streptococcus (GBS) colonisation is dynamic over time, whilst GBS capsular polysaccharides-specific antibody remains stable\nI.L. Haeusler1*, O. Daniel1*, C. Isitt4*, R. Watts1, L. Cantrell2, S. Feng2, M. Cochet1, M. Salloum1,3, S. Ikram4, E. Hayter4, S. Lim1, T. Hall1, S. Athaide4, C.A. Cosgrove4^, J.S. Tregoning5^, K. Le Doare1,6,7^\n1St George's University of London, Paediatric Infectious Diseases Research Group, London, United Kingdom; 2Oxford Vaccine Group, Department of Paediatrics, University of Oxford; 3UnivLyon, Claude Bernard University Lyon I, France; 4St George's University of London, The Vaccine Institute, London, United Kingdom; 5Imperial College London, Department of Infectious Disease, London, United Kingdom; 6Makerere University John Hopkins Research Collaboration, Kampala, Uganda; 7Pathogen Immunology Group, United Kingdom Health Security Agency, Porton Down, United Kingdom.\n*,^Contributed equally\nCorresponding author: Olwenn Daniel (\u0013 HYPERLINK \"mailto:odaniel@sgul.ac.uk\" \u0014odaniel@sgul.ac.uk\u0015)\u000f\nStatements\nData availability statement\nData are available in the St George’s Research Data Repository (\u0013 HYPERLINK \"https://sgul.figshare.com\" \u0014https://sgul.figshare.com\u0015).\nFunding\nThis work was supported by the Human Infection Challenge Network for Vaccine Development (HIC-Vac) (grant number MR/R005982/1) funded by the GCRF Networks in Vaccines Research and Development which was co-funded by the MRC and BBSRC. This UK funded award is part of the EDCTP2 programme supported by the European Union.\nThis work was also supported by the UKRI Fellowship [grant number MR/S016570/1].\nMS was registered in the EMJMD LIVE (Erasmus+ Mundus joint Master Degree Leading International Vaccinology Education), co-funded by the EACEA (Education, Audiovisual and Culture Executive Agency, award 2018-1484) of the European Commission and received a scholarship form the EACEA.\nThe funders played no role in the design, implementation, analysis, interpretation nor publication of the study.\nConflict of interests\nThe authors have declared no conflicts of interest.\nEthics approval and patient consent\nThe study was approved by the HRA and HCRW (Health Research Authority and Health and Care Research Wales, IRAS number 261556). Participants provided written informed consent prior to inclusion in the study and were able to leave the study at any time without consequence.\nClinical trial registration\nTrial registry identification NCT04059510 (\u0013 HYPERLINK \"http://www.clinicaltrials.gov\" \u0014www.clinicaltrials.gov\u0015).\nAcknowledgments\nWe thank the participants for their involvement in this study.\nCapsular polysaccharides poly-l-lysine conjugates were kindly provided by Pfizer.\nGraphical abstract created with\u0013 HYPERLINK \"https://biorender.com/\" \\t \"_blank\" \u0014 BioRender.com\u0015.\nAuthor contributions\nKLD, JT, CC and CI conceived the study and designed the study methods. OD, CI, EH, RW, MC, SI, SL, SA and TH collected data. ILH and OD entered and cleaned the data. OD, RW, MS performed the laboratory analysis. SL and TH oversaw laboratory analysis and quality control. ILH, OD, LC, and SF performed the statistical analysis. ILH and OD wrote the first draft of the manuscript. All authors reviewed the manuscript and approved the final version.\nKeywords\nGroup B Streptococcus; neonatal infection; colonisation; mucosal immunity; capsular-polysaccharides specific antibodies; GBS vaccine.\u000f\nAbstract\nGroup B Streptococcus (GBS) is a leading cause of adverse pregnancy outcomes due to invasive infection. This study investigated longitudinal variation in GBS rectovaginal colonisation, serum and vaginal GBS capsular polysaccharide (CPS)-specific antibody levels.\nNon-pregnant women were recruited in the UK, and were sampled every two weeks over a 12-week period. GBS isolates were taken from recto-vaginal swabs and serotyped by polymerase chain reaction. Serum and vaginal immunoglobulin G (IgG) and nasal immunoglobulin A (IgA) specific to CPS were measured by Luminex, and total ","cbCaioROnVwHNQVp","https://ap.wps.com/l/cbCaioROnVwHNQVp","docx",908572,35,"English","# Abstract\n## Study aims and design\n## Participant sampling and assays\n## Key findings on colonisation vs antibodies\n## Implications for vaccine development","[{\"question\":\"What does the study examine about Group B Streptococcus during follow-up?\",\"answer\":\"It examines longitudinal variation in GBS rectovaginal colonisation and CPS-specific antibody levels in serum and vaginal compartments over a 12-week period.\"},{\"question\":\"How were participants and samples collected and analyzed?\",\"answer\":\"Non-pregnant women were sampled every two weeks for 12 weeks. GBS isolates from recto-vaginal swabs were PCR serotyped, and CPS-specific IgG/IgA and total IgG/A were measured using Luminex and ELISA.\"},{\"question\":\"What is the main relationship between GBS colonisation dynamics and CPS-specific antibody levels?\",\"answer\":\"Although detectable GBS carriage fluctuated over time, CPS-specific antibody levels in serum, vaginal tissue, and nasal measures remained constant.\"}]","Group B Streptococcus (GBS) colonisation dynamics and CPS-specific antibody stability | DOCX",1788328508]