[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"detail-sidebar-cat-1-en-105":3,"doc-seo-170736-105":53,"doc-detail-170736-en":126},{"code":4,"msg":5,"data":6},0,"success",[7,14,19,24,29,34,39,44,49],{"id":8,"doc_module":9,"doc_module_name":10,"category_name":11,"show_sort_weight":12,"slug":13},11,1,"Template","Presentations",90,"presentations",{"id":15,"doc_module":9,"doc_module_name":10,"category_name":16,"show_sort_weight":17,"slug":18},12,"Resumes",80,"resumes",{"id":20,"doc_module":9,"doc_module_name":10,"category_name":21,"show_sort_weight":22,"slug":23},14,"Invoices",70,"invoices",{"id":25,"doc_module":9,"doc_module_name":10,"category_name":26,"show_sort_weight":27,"slug":28},15,"Posters",60,"posters",{"id":30,"doc_module":9,"doc_module_name":10,"category_name":31,"show_sort_weight":32,"slug":33},16,"Social Media",50,"social-media",{"id":35,"doc_module":9,"doc_module_name":10,"category_name":36,"show_sort_weight":37,"slug":38},17,"Forms",40,"forms",{"id":40,"doc_module":9,"doc_module_name":10,"category_name":41,"show_sort_weight":42,"slug":43},18,"Letters",30,"letters",{"id":45,"doc_module":9,"doc_module_name":10,"category_name":46,"show_sort_weight":47,"slug":48},21,"Paper Templates",5,"papers-templates",{"id":50,"doc_module":9,"doc_module_name":10,"category_name":51,"show_sort_weight":4,"slug":52},158,"General","general-158",{"code":4,"msg":54,"data":55},"ok",{"site_id":56,"language":57,"slug":58,"title":59,"keywords":60,"description":61,"schema_data":62,"social_meta":119,"head_meta":121,"extra_data":123,"updated_unix":125},105,"en","filmarray-gastrointestinal-panel-testing-procedure-cary-blair-transport-media-clsi-template","FilmArray Gastrointestinal Panel Testing - Procedure - Cary Blair Transport Media - CLSI Template","","Procedure for testing human stool specimens collected in Cary Blair transport medium using the FilmArray Gastrointestinal (GI) Panel kit on the FilmArray Instrument. Describes the multiplex nucleic acid workflow for qualitative detection of multiple gastrointestinal pathogens, including required specimen volume, processing and storage timing, and run automation. Explains the pouch chemistry for nucleic acid purification, reverse transcription for RNA viruses, two-stage multiplex PCR, DNA melting analysis, and software-generated reporting. Covers built-in process controls and how control failures affect validity.",{"@graph":63,"@context":118},[64,80,101],{"@type":65,"itemListElement":66},"BreadcrumbList",[67,71,74,77],{"item":68,"name":69,"@type":70,"position":9},"https://docshare.wps.com","Home","ListItem",{"item":72,"name":10,"@type":70,"position":73},"https://docshare.wps.com/template/",2,{"item":75,"name":36,"@type":70,"position":76},"https://docshare.wps.com/template/forms/",3,{"item":78,"name":59,"@type":70,"position":79},"https://docshare.wps.com/template/filmarray-gastrointestinal-panel-testing-procedure-cary-blair-transport-media-clsi-template/170736/",4,{"url":78,"name":59,"@type":81,"image":82,"author":87,"headline":59,"publisher":90,"fileFormat":93,"inLanguage":57,"description":61,"dateModified":94,"datePublished":95,"encodingFormat":93,"isAccessibleForFree":96,"interactionStatistic":97},"DigitalDocument",{"url":83,"@type":84,"width":85,"height":86},"https://docshare.wps.com/thumbnails/filmarray-gastrointestinal-panel-testing-procedure-cary-blair-transport-media-clsi-template/170736.png","ImageObject",442,249,{"name":88,"@type":89},"Noah","Person",{"url":68,"name":91,"@type":92},"DocShare","Organization","application/vnd.openxmlformats-officedocument.wordprocessingml.document","2026-10-24","2026-09-01",true,{"@type":98,"interactionType":99,"userInteractionCount":79},"InteractionCounter",{"@type":100},"ViewAction",{"@type":102,"mainEntity":103},"FAQPage",[104,110,114],{"name":105,"@type":106,"acceptedAnswer":107},"What stool transport medium and specimen volume are required for FilmArray GI testing?","Question",{"text":108,"@type":109},"Use human stool collected in Cary Blair transport medium. Testing requires 200 µL of sample.","Answer",{"name":111,"@type":106,"acceptedAnswer":112},"How does the FilmArray GI Panel detect multiple pathogens from one stool specimen?",{"text":113,"@type":109},"The pouch is a closed system that automates nucleic acid purification, reverse transcription for RNA viruses, two-stage multiplex PCR, and DNA melting analysis. FilmArray Software analyzes replicate wells and generates the test report.",{"name":115,"@type":106,"acceptedAnswer":116},"What happens if the FilmArray process controls fail during a run?",{"text":117,"@type":109},"Both the RNA Process Control and PCR2 Control must be positive for the run to pass. If either control fails, the controls field shows Failed and all results are listed as Invalid; the sample should be retested using a new pouch.","https://schema.org",{"og:url":78,"og:type":120,"og:title":59,"og:site_name":91,"og:description":61},"article",{"robots":122,"canonical":78},"index,follow",{"doc_id":124,"site_id":56},170736,1792223478,{"code":4,"msg":5,"data":127},{"doc_id":124,"user_id":128,"nickname":88,"user_avatar":129,"doc_module":9,"category_id":35,"category_name":36,"doc_title":59,"doc_description":61,"doc_content":130,"file_id":131,"file_url":132,"file_type":133,"file_size":134,"view_count":79,"is_deleted":4,"is_public":9,"is_downloadable":9,"audit_status":9,"page_count":135,"language":136,"language_code":57,"site_id":56,"html_lang":57,"table_of_contents":137,"faqs":138,"seo_title":139,"seo_description":61,"update_tm":140,"read_time":47},8796095462418,"https://ap-avatar.wpscdn.com/avatar/80000253c1241d02b47?x-image-process=image/resize,m_fixed,w_180,h_180&k=1778826106357471780","FilmArray® Gastrointestinal Panel Testing\nPurpose\nThis procedure provides instructions for testing stool samples in Cary Blair transport media using the FilmArray Gastrointestinal Panel (GI) Kit.\nBackground\nThe FilmArray GI is a multiplexed nucleic acid test intended for use with the FilmArray Instrument for the simultaneous qualitative detection and identification of nucleic acids from multiple bacteria, viruses, and parasites directly from stool samples in Cary Blair transport media obtained from individuals with signs and/or symptoms of gastrointestinal infection.\nThe following bacteria (including several diarrheagenic E. coli/Shigella pathotypes), parasites, and viruses are identified using the FilmArray GI Panel:\nCampylobacter (C. jejuni/C. coli/C. upsaliensis)\nClostridium difficile (C. difficile) toxin A/B\nPlesiomonas shigelloides\nSalmonella\nVibrio (V. parahaemolyticus/V. vulnificus/ V. cholerae), including specific identification of Vibrio cholerae\nYersinia enterocolitica\nEnteroaggregative Escherichia coli (EAEC)\nEnteropathogenic Escherichia coli (EPEC)\nEnterotoxigenic Escherichia coli (ETEC) lt/st\nShiga-like toxin-producing Escherichia coli (STEC) stx1/stx2 (including specific identification of the E. coli O157 serogroup within STEC)\nShigella/ Enteroinvasive Escherichia coli (EIEC)\nCryptosporidium\nCyclospora cayetanensis\nEntamoeba histolytica\nGiardia lamblia (also known as G. intestinalis and G. duodenalis)\nAdenovirus F 40/41\nAstrovirus\nNorovirus GI/GII\nRotavirus A\nSapovirus (Genogroups I, II, IV, and V)\nPrinciple of the Procedure\nThe FilmArray GI pouch is a closed system disposable that houses all the chemistry required to isolate, amplify and detect nucleic acid from multiple gastrointestinal pathogens within a single stool specimen. The rigid plastic component (fitment) of the FilmArray GI pouch contains reagents in freeze-dried form. The flexible plastic portion of the pouch is divided into discrete segments (blisters) where the required chemical processes are carried out. The user of the FilmArray GI Panel loads the sample into the FilmArray GI pouch, places the pouch into the FilmArray Instrument, and starts the run. All other operations are automated.\nThe following is an overview of the operations and processes that occur during a FilmArray run:\n1. Nucleic Acid Purification - Nucleic acid purification occurs in the first three blisters of the pouch. The sample is lysed by a combination of chemical and mechanical (bead beating) mechanisms and the liberated nucleic acid is captured, washed and eluted using magnetic bead technology. These steps require about ten minutes, and the bead-beater apparatus can be heard as a high-pitched whine during the first few minutes of operation.\n2. Reverse Transcription and 1st Stage Multiplex PCR - Since the GI Panel includes RNA viruses, a reverse transcription (RT) step is performed to convert the viral RNA into cDNA prior to amplification. The purified nucleic acid solution is combined with a preheated master mix to initiate the RT step and subsequent thermocycling for multiplex PCR. The effect of 1st stage PCR is to enrich for the target nucleic acids present in the sample.\n3. 2nd Stage PCR - The products of 1st stage PCR are diluted and mixed with fresh PCR reagents containing an intercalating fluorescent DNA dye (LCGreen® Plus, BioFire Diagnostics). This solution is distributed over the 2nd stage PCR array. The individual wells of the array contain primers for different assays (each present in triplicate) that target specific nucleic acid sequences from each of the pathogens detected, as well as control template material. These primers are ‘nested’ or internal to the specific products of the 1st stage multiplex reaction, which enhances both the sensitivity and specificity of the reactions.\n4. DNA Melting Analysis – After 2nd stage PCR, the temperature is slowly increased and fluorescence in each well of the array is monitored and analyzed to generate a melt curve. The temper","cbCaihk5d8r8QOZr","https://ap.wps.com/l/cbCaihk5d8r8QOZr","docx",47590,13,"English","# Purpose\n# Background\n# Principle of the Procedure\n## Nucleic Acid Purification\n## Reverse Transcription and 1st Stage Multiplex PCR\n## 2nd Stage PCR\n## DNA Melting Analysis\n# Specimen\n# Materials\n# Quality Control\n## Process Controls\n## Monitoring Test System Performance","[{\"question\":\"What stool transport medium and specimen volume are required for FilmArray GI testing?\",\"answer\":\"Use human stool collected in Cary Blair transport medium. Testing requires 200 µL of sample.\"},{\"question\":\"How does the FilmArray GI Panel detect multiple pathogens from one stool specimen?\",\"answer\":\"The pouch is a closed system that automates nucleic acid purification, reverse transcription for RNA viruses, two-stage multiplex PCR, and DNA melting analysis. FilmArray Software analyzes replicate wells and generates the test report.\"},{\"question\":\"What happens if the FilmArray process controls fail during a run?\",\"answer\":\"Both the RNA Process Control and PCR2 Control must be positive for the run to pass. If either control fails, the controls field shows Failed and all results are listed as Invalid; the sample should be retested using a new pouch.\"}]","FilmArray Gastrointestinal Panel Testing - Procedure - Cary Blair Transport Media - CLSI Template | DOCX",1788274740]