[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"doc-detail-165123-en":3,"doc-seo-165123-105":30,"detail-sidebar-cat-1-en-105":91},{"code":4,"msg":5,"data":6},0,"success",{"doc_id":7,"user_id":8,"nickname":9,"user_avatar":10,"doc_module":11,"category_id":12,"category_name":13,"doc_title":14,"doc_description":15,"doc_content":16,"file_id":17,"file_url":18,"file_type":19,"file_size":20,"view_count":4,"is_deleted":4,"is_public":11,"is_downloadable":11,"audit_status":11,"page_count":21,"language":22,"language_code":23,"site_id":24,"html_lang":23,"table_of_contents":25,"faqs":26,"seo_title":27,"seo_description":15,"update_tm":28,"read_time":29},165123,1374391974564,"Clementine","https://ap-avatar.wpscdn.com/avatar/14000253aa45c000a9e?x-image-process=image/resize,m_fixed,w_180,h_180&k=1779874745381141002",1,11,"Presentations","Data on cytotoxic activity of an Artemisia annua herbal preparation - Supporting information and validation of quantification method","The data in this article provide supporting information for an antitumor research manuscript focused on Artemisia annua herbal preparations. The work describes how the Momundo extract and its acetonitrile fraction induce apoptosis in MDA-MB-231 triple negative breast cancer cells, including viability changes by XTT assays and subG0/G1 shifts by flow cytometry. It also presents the HPLC-DAD characterization workflow and the UHPLC-MS/MS quantification approach, including validation details for abundant active compounds.","DATA IN BRIEF TEMPLATE\nMeta-Data (Mandatory information required for the transfer of your article to Data in Brief – will not be typeset)\nData Article\nTitle: Data on cytotoxic activity of an Artemisia annua herbal preparation and validation of the quantification method for active ingredient analysis\nAuthors: Michael Schmiech, Sophia J. Lang, Tatiana Syrovets, Thomas Simmet\nAffiliation:\nInstitute of Pharmacology of Natural Products and Clinical Pharmacology, Ulm University, 89081 Ulm, Germany\nContact email: thomas.simmet@uni-ulm.de\n\u000f\nAbstract\nThe data in this article contain supporting information for the research manuscript entitled “Antitumor activity of an Artemisia annua herbal preparation and identification of active ingredients” by Lang et al. \u0013 ADDIN EN.CITE \u003CEndNote>\u003CCite>\u003CAuthor>Lang\u003C/Author>\u003CYear>2019\u003C/Year>\u003CRecNum>46\u003C/RecNum>\u003CDisplayText>(Lang et al., 2019)\u003C/DisplayText>\u003Crecord>\u003Crec-number>46\u003C/rec-number>\u003Cforeign-keys>\u003Ckey app=\"EN\" db-id=\"rpzt9rpf90vsv0ewzf6pssvbpfwepe0adwp2\" timestamp=\"1556645616\">46\u003C/key>\u003C/foreign-keys>\u003Cref-type name=\"Journal Article\">17\u003C/ref-type>\u003Ccontributors>\u003Cauthors>\u003Cauthor>Lang, S..J. \u003C/author>\u003Cauthor>Schmiech, M.\u003C/author>\u003Cauthor>Hafner, S.\u003C/author>\u003Cauthor>Paetz, C.\u003C/author>\u003Cauthor>Steinborn, C.\u003C/author>\u003Cauthor> Huber, R.\u003C/author>\u003Cauthor>El Gaafary, M.\u003C/author>\u003Cauthor>Werner, K.,\u003C/author>\u003Cauthor>Schmidt, C.Q. \u003C/author>\u003Cauthor>Syrovets, T. \u003C/author>\u003Cauthor>Simmet, T.\u003C/author>\u003C/authors>\u003C/contributors>\u003Ctitles>\u003Ctitle>Antitumor activity of an Artemisia annua herbal preparation and identification of active ingredients\u003C/title>\u003Csecondary-title>Phytomedicine\u003C/secondary-title>\u003C/titles>\u003Cperiodical>\u003Cfull-title>Phytomedicine\u003C/full-title>\u003C/periodical>\u003Cvolume>in press\u003C/volume>\u003Cdates>\u003Cyear>2019\u003C/year>\u003C/dates>\u003Curls>\u003C/urls>\u003C/record>\u003C/Cite>\u003C/EndNote>\u0014(Lang et al., 2019)\u0015. Momundo Artemisia annua extract and an acetonitrile fraction thereof induce apoptosis in MDA-MB-231 triple negative breast cancer cells as shown by XTT viability assay and induction of the subG0/G1 cell population by flow cytometric analysis. Furthermore, the HPLC-DAD method used to characterize the Artemisia annua herbal preparation as well as UHPLC-MS/MS method used to quantify the most abundant compounds in the extract and its validation are presented.\nSpecifications Table\nValue of the Data\nThe data provide additional information on the molecular mechanisms of action of the Artemisia annua extract Momundo and its cytotoxic and proapoptotic activity against chemoresistant triple negative human breast cancer cells\nChemical characterization of the extract and validation method for the quantification of the most abundant compounds facilitate identification of biologically active compounds in plant extracts, in general, and Artemisia annua, in particular\nThis work supports discovery of new chemical entities of natural origin and identification of their molecular mechanisms of action with the aim to develop candidates for anticancer therapy\nData\nInduction of apoptosis by Artemisia annua herbal preparations\nInduction of apoptosis was analyzed by DNA-staining with propidium iodide followed by flow cytometry \u0013 ADDIN EN.CITE \u003CEndNote>\u003CCite>\u003CAuthor>Riccardi\u003C/Author>\u003CYear>2006\u003C/Year>\u003CRecNum>8200\u003C/RecNum>\u003CDisplayText>(Riccardi and Nicoletti, 2006)\u003C/DisplayText>\u003Crecord>\u003Crec-number>8200\u003C/rec-number>\u003Cforeign-keys>\u003Ckey app=\"EN\" db-id=\"wtsvt5vvjdapsze0pphvtf2etrvtsdf0tpxv\" timestamp=\"1549536255\">8200\u003C/key>\u003Ckey app=\"ENWeb\" db-id=\"\">0\u003C/key>\u003C/foreign-keys>\u003Cref-type name=\"Journal Article\">17\u003C/ref-type>\u003Ccontributors>\u003Cauthors>\u003Cauthor>Riccardi, C.\u003C/author>\u003Cauthor>Nicoletti, I.\u003C/author>\u003C/authors>\u003C/contributors>\u003Cauth-address>Department of Clinical and Experimental Medicine, School of Medicine, University of Perugia, and Foundation IBiT, 06122 Perugia, Italy. riccardi@unipg.it\u003C/auth-address>\u003Ctitles>\u003Ctitle>Analysis of apoptosis by propidium iodide staining and flow cytometry\u003C/title>\u003Csecondary-title>Nat Protoc\u003C/secondary-title>\u003C/titles>\u003Cperiodical>\u003Cfull-title>Nat Protoc\u003C","cbCaimp3CHrMVUFF","https://ap.wps.com/l/cbCaimp3CHrMVUFF","docx",422032,8,"English","en",105,"# Abstract\n# Specifications Table\n## Value of the Data\n## Induction of apoptosis by Artemisia annua herbal preparations\n## Purification of compounds from Momundo extract","[{\"question\":\"What research topic do these data support?\",\"answer\":\"The dataset supports research on antitumor activity of an Artemisia annua herbal preparation, including identification of active ingredients and related mechanistic findings.\"},{\"question\":\"How is apoptosis induced and measured in the described experiments?\",\"answer\":\"The Momundo extract and its acetonitrile fraction are used to treat MDA-MB-231 cells, and apoptotic cells are quantified after DNA staining with propidium iodide using flow cytometry and DNA-fluorescence reduction. FlowJo software is referenced for quantification.\"},{\"question\":\"Which analytical methods are used for chemical characterization and quantification?\",\"answer\":\"The extract is characterized using an HPLC-DAD method, while the most abundant compounds are quantified using an UHPLC-MS/MS method, with validation information provided in the dataset.\"}]","Data on cytotoxic activity of an Artemisia annua herbal preparation - Supporting information and validation of quantification method | DOCX",1788166480,3,{"code":4,"msg":31,"data":32},"ok",{"site_id":24,"language":23,"slug":33,"title":14,"keywords":34,"description":15,"schema_data":35,"social_meta":86,"head_meta":88,"extra_data":90,"updated_unix":28},"data-on-cytotoxic-activity-of-an-artemisia-annua-herbal-preparation-supporting-information-and-validation-of-quantification-method","",{"@graph":36,"@context":85},[37,53,68],{"@type":38,"itemListElement":39},"BreadcrumbList",[40,44,48,50],{"item":41,"name":42,"@type":43,"position":11},"https://docshare.wps.com","Home","ListItem",{"item":45,"name":46,"@type":43,"position":47},"https://docshare.wps.com/template/","Template",2,{"item":49,"name":13,"@type":43,"position":29},"https://docshare.wps.com/template/presentations/",{"item":51,"name":14,"@type":43,"position":52},"https://docshare.wps.com/template/data-on-cytotoxic-activity-of-an-artemisia-annua-herbal-preparation-supporting-information-and-validation-of-quantification-method/165123/",4,{"url":51,"name":14,"@type":54,"author":55,"headline":14,"publisher":57,"fileFormat":60,"inLanguage":23,"description":15,"dateModified":61,"datePublished":62,"encodingFormat":60,"isAccessibleForFree":63,"interactionStatistic":64},"DigitalDocument",{"name":9,"@type":56},"Person",{"url":41,"name":58,"@type":59},"DocShare","Organization","application/vnd.openxmlformats-officedocument.wordprocessingml.document","2026-09-03","2026-08-31",true,{"@type":65,"interactionType":66,"userInteractionCount":47},"InteractionCounter",{"@type":67},"ViewAction",{"@type":69,"mainEntity":70},"FAQPage",[71,77,81],{"name":72,"@type":73,"acceptedAnswer":74},"What research topic do these data support?","Question",{"text":75,"@type":76},"The dataset supports research on antitumor activity of an Artemisia annua herbal preparation, including identification of active ingredients and related mechanistic findings.","Answer",{"name":78,"@type":73,"acceptedAnswer":79},"How is apoptosis induced and measured in the described experiments?",{"text":80,"@type":76},"The Momundo extract and its acetonitrile fraction are used to treat MDA-MB-231 cells, and apoptotic cells are quantified after DNA staining with propidium iodide using flow cytometry and DNA-fluorescence reduction. 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