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Includes sections for research description and protocol-specific risk assessment, required personnel training (EHS-recommended modules and competency demonstration), and detailed laboratory signage practices with permanent and alternative postings. Provides guidance for biosafety cabinet use, safe handling and disposal expectations, spill response, emergency procedures, post-exposure actions, animal work, cryogenic liquid safety, and appendices for contacts, references, and training records.",{"@graph":63,"@context":118},[64,80,101],{"@type":65,"itemListElement":66},"BreadcrumbList",[67,71,74,77],{"item":68,"name":69,"@type":70,"position":9},"https://docshare.wps.com","Home","ListItem",{"item":72,"name":10,"@type":70,"position":73},"https://docshare.wps.com/template/",2,{"item":75,"name":36,"@type":70,"position":76},"https://docshare.wps.com/template/forms/",3,{"item":78,"name":59,"@type":70,"position":79},"https://docshare.wps.com/template/bsl-2-biosafety-manual-lab-specific-template-training-signage-cabinet-and-disposal/164737/",4,{"url":78,"name":59,"@type":81,"image":82,"author":87,"headline":59,"publisher":90,"fileFormat":93,"inLanguage":57,"description":61,"dateModified":94,"datePublished":95,"encodingFormat":93,"isAccessibleForFree":96,"interactionStatistic":97},"DigitalDocument",{"url":83,"@type":84,"width":85,"height":86},"https://docshare.wps.com/thumbnails/bsl-2-biosafety-manual-lab-specific-template-training-signage-cabinet-and-disposal/164737.png","ImageObject",442,249,{"name":88,"@type":89},"Asher","Person",{"url":68,"name":91,"@type":92},"DocShare","Organization","application/vnd.openxmlformats-officedocument.wordprocessingml.document","2026-09-23","2026-08-31",true,{"@type":98,"interactionType":99,"userInteractionCount":47},"InteractionCounter",{"@type":100},"ViewAction",{"@type":102,"mainEntity":103},"FAQPage",[104,110,114],{"name":105,"@type":106,"acceptedAnswer":107},"What must be included in the research introduction and protocol-specific risk assessment?","Question",{"text":108,"@type":109},"The template requires a brief description of the research, a list of biological agents/organisms used, and a protocol-specific risk assessment addressing factors such as pathogenicity, virulence, infectious dose, route of transmission, host range, stability, exposure likelihood, and exposure consequences. It also asks how identified risks will be controlled through work practices and PPE.","Answer",{"name":111,"@type":106,"acceptedAnswer":112},"What training is required for laboratory personnel working under BSL-2?",{"text":113,"@type":109},"Personnel must receive adequate instruction from their supervisor before starting work, with some training required annually. EHS recommends specific BSL-2 training topics including Bloodborne Pathogens, NIH recombinant/synthetic nucleic acid guidelines, safe animal work, spill prevention and countermeasures, cryogenic liquid safety, hazardous waste generator training, and shipping infectious materials training when applicable.",{"name":115,"@type":106,"acceptedAnswer":116},"How should laboratory signage be handled for BSL-2 work?",{"text":117,"@type":109},"Permanent yellow warning signs with the biohazard symbol and BSL-2 designation must be posted at entrances where Risk Group 2 agents are used or stored long-term. Biosafety level 2 labs should also use permanently affixed decals for the biohazard symbol, “BSL-2,” and exit requirements. Alternative supplemental signage is used when the agent will be removed and the room decontaminated frequently, and the biohazard/BSL-2 markings are removed from the permanent sign when that system is active.","https://schema.org",{"og:url":78,"og:type":120,"og:title":59,"og:site_name":91,"og:description":61},"article",{"robots":122,"canonical":78},"index,follow",{"doc_id":124,"site_id":56},164737,1788160001,{"code":4,"msg":5,"data":127},{"doc_id":124,"user_id":128,"nickname":88,"user_avatar":129,"doc_module":9,"category_id":35,"category_name":36,"doc_title":59,"doc_description":61,"doc_content":130,"file_id":131,"file_url":132,"file_type":133,"file_size":134,"view_count":47,"is_deleted":4,"is_public":9,"is_downloadable":9,"audit_status":9,"page_count":135,"language":136,"language_code":57,"site_id":56,"html_lang":57,"table_of_contents":137,"faqs":138,"seo_title":139,"seo_description":61,"update_tm":125,"read_time":140},687197207639,"https://ap-avatar.wpscdn.com/davatar_a8503ba1806abce46bf441b54a3ca4cd","ENTITY/UNIVERSITY\nBSL-2 BIOSAFETY MANUAL\nDr. ___’s Lab\n___ Building\nRooms ___\n\u000f\n\u0003Contents\n\u0013 TOC \\o \"1-3\" \\h \\z \\u \u0014\u0013 HYPERLINK \\l \"_Toc330369751\" \u0014I. INTRODUCTION AND DESCRIPTION OF RESEARCH\t\u0013 PAGEREF _Toc330369751 \\h \u00143\u0015\u0015\n\u0013 HYPERLINK \\l \"_Toc330369752\" \u0014II. TRAINING\t\u0013 PAGEREF _Toc330369752 \\h \u00144\u0015\u0015\n\u0013 HYPERLINK \\l \"_Toc330369753\" \u0014III. LABORATORY SIGNAGE\t\u0013 PAGEREF _Toc330369753 \\h \u00145\u0015\u0015\n\u0013 HYPERLINK \\l \"_Toc330369754\" \u0014IV. USE OF BIOLOGICAL SAFETY CABINET\t\u0013 PAGEREF _Toc330369754 \\h \u00146\u0015\u0015\n\u0013 HYPERLINK \\l \"_Toc330369755\" \u0014V. USE AND DISPOSAL OF SHARPS\t\u0013 PAGEREF _Toc330369755 \\h \u00148\u0015\u0015\n\u0013 HYPERLINK \\l \"_Toc330369756\" \u0014VI. SPILL RESPONSE PROCEDURES\t\u0013 PAGEREF _Toc330369756 \\h \u001410\u0015\u0015\n\u0013 HYPERLINK \\l \"_Toc330369757\" \u0014VII. WASTE DISPOSAL PROCEDURES\t\u0013 PAGEREF _Toc330369757 \\h \u001411\u0015\u0015\n\u0013 HYPERLINK \\l \"_Toc330369758\" \u0014VIII. EMERGENCY PROCEDURES\t\u0013 PAGEREF _Toc330369758 \\h \u001412\u0015\u0015\n\u0013 HYPERLINK \\l \"_Toc330369759\" \u0014IX. Post-Exposure Fact Sheet\t\u0013 PAGEREF _Toc330369759 \\h \u001413\u0015\u0015\n\u0013 HYPERLINK \\l \"_Toc330369760\" \u0014X. WORKING WITH ANIMALS\t\u0013 PAGEREF _Toc330369760 \\h \u001414\u0015\u0015\n\u0013 HYPERLINK \\l \"_Toc330369761\" \u0014XI. SAFE HANDLING OF CRYOGENIC LIQUIDS\t\u0013 PAGEREF _Toc330369761 \\h \u001415\u0015\u0015\n\u0013 HYPERLINK \\l \"_Toc330369762\" \u0014APPENDIX 1: EMERGENCY CONTACTS\t\u0013 PAGEREF _Toc330369762 \\h \u001416\u0015\u0015\n\u0013 HYPERLINK \\l \"_Toc330369763\" \u0014APPENDIX 2: REFERENCES\t\u0013 PAGEREF _Toc330369763 \\h \u001417\u0015\u0015\n\u0013 HYPERLINK \\l \"_Toc330369764\" \u0014APPENDIX 3: AGENT SUMMARY STATEMENT (OPTIONAL)\t\u0013 PAGEREF _Toc330369764 \\h \u001418\u0015\u0015\n\u0013 HYPERLINK \\l \"_Toc330369765\" \u0014Appendix 4. BMBL BSL-2 Laboratory Criteria\t\u0013 PAGEREF _Toc330369765 \\h \u001419\u0015\u0015\n\u0013 HYPERLINK \\l \"_Toc330369766\" \u0014Appendix 5. Lab Staff Training Records\t\u0013 PAGEREF _Toc330369766 \\h \u001424\u0015\u0015\n\u0013 HYPERLINK \\l \"_Toc330369767\" \u0014Appendix 6. Principal Investigator’s Registered Research\t\u0013 PAGEREF _Toc330369767 \\h \u001425\u0015\u0015\n\u0015\u0004\n\u000f\nI. INTRODUCTION AND DESCRIPTION OF RESEARCH\nInsert brief description of research.\nBiological agents/organisms used:\n(include bacterial, viral, fungal, recombinant, human/non-human primate unfixed tissues or cells)\nList organisms/biohazards here\nRisk Assessment:\nPlease provide a brief protocol-specific risk assessment. Include consideration of parent and recombinant agent pathogenicity, virulence, infectious dose, route of transmission, host range, and stability, as well as the likelihood of exposure and consequences of exposure. How will identified risks be controlled (e.g. PPE, work practices, etc.)?  Note that “replication incompetent” does not mean “non-infectious.”\nResearch involving recombinant/synthetic nucleic acids, infectious agents, creation of transgenic animals, unfixed non-human primate materials, or human blood, cells, or unfixed tissues must submit a research registration form through EHS at least every four years.  This work may require prior review and approval by the Institutional Biosafety Committee, according to the NIH Guidelines for Research Involving Recombinant DNA Molecules.  Since the university receives funding from NIH, all research at UM must comply with the NIH Guidelines.\nThis plan was designed as a supplement to the University Biosafety Manual to meet the requirements of Biosafety in Microbiological and Biomedical Laboratories, 5th ed.\n\u000f\nII. TRAINING\nAll personnel who work in the laboratory must receive adequate instruction from their supervisor prior to beginning work.  Some training is required annually.  Each lab will require different trainings.  For biosafety, EHS recommends:\nBSL2 training\nBloodborne Pathogens training\nNIH Guidelines for Research Involving Recombinant/Synthetic Nucleic Acid Molecules\nWorking Safely With Laboratory Animals\nSpill Prevention, Control, and Countermeasure\nSafe Handling and Use of Cryogenic Liquids\nHazardous Waste Generator\nShipping Infectious Materials: Training is required to commercially transport infectious materials.  Contact EHS to schedule a training class.\nTraining on lab-specific techniques and demonstration of competency should also be required before work.  Some work may require an occupational heal","cbCaivS9XC5pV1G3","https://ap.wps.com/l/cbCaivS9XC5pV1G3","docx",388249,24,"English","# I. INTRODUCTION AND DESCRIPTION OF RESEARCH\n# II. TRAINING\n# III. LABORATORY SIGNAGE\n# IV. USE OF BIOLOGICAL SAFETY CABINET\n# V. USE AND DISPOSAL OF SHARPS\n# VI. SPILL RESPONSE PROCEDURES\n# VII. WASTE DISPOSAL PROCEDURES\n# VIII. EMERGENCY PROCEDURES\n# IX. Post-Exposure Fact Sheet\n# X. WORKING WITH ANIMALS\n# XI. SAFE HANDLING OF CRYOGENIC LIQUIDS\n# APPENDIX 1: EMERGENCY CONTACTS\n# APPENDIX 2: REFERENCES\n# APPENDIX 3: AGENT SUMMARY STATEMENT (OPTIONAL)\n# Appendix 4. BMBL BSL-2 Laboratory Criteria\n# Appendix 5. Lab Staff Training Records\n# Appendix 6. Principal Investigator’s Registered Research","[{\"question\":\"What must be included in the research introduction and protocol-specific risk assessment?\",\"answer\":\"The template requires a brief description of the research, a list of biological agents/organisms used, and a protocol-specific risk assessment addressing factors such as pathogenicity, virulence, infectious dose, route of transmission, host range, stability, exposure likelihood, and exposure consequences. It also asks how identified risks will be controlled through work practices and PPE.\"},{\"question\":\"What training is required for laboratory personnel working under BSL-2?\",\"answer\":\"Personnel must receive adequate instruction from their supervisor before starting work, with some training required annually. EHS recommends specific BSL-2 training topics including Bloodborne Pathogens, NIH recombinant/synthetic nucleic acid guidelines, safe animal work, spill prevention and countermeasures, cryogenic liquid safety, hazardous waste generator training, and shipping infectious materials training when applicable.\"},{\"question\":\"How should laboratory signage be handled for BSL-2 work?\",\"answer\":\"Permanent yellow warning signs with the biohazard symbol and BSL-2 designation must be posted at entrances where Risk Group 2 agents are used or stored long-term. Biosafety level 2 labs should also use permanently affixed decals for the biohazard symbol, “BSL-2,” and exit requirements. Alternative supplemental signage is used when the agent will be removed and the room decontaminated frequently, and the biohazard/BSL-2 markings are removed from the permanent sign when that system is active.\"}]","BSL-2 Biosafety Manual - Lab-Specific Template - Training, Signage, Cabinet, and Disposal | DOCX",8]