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The results indicate significant alterations in liver enzyme levels, with KO mice showing elevated ALP, ALT, and AST, suggesting liver damage. Hematoxylin and eosin (H&E) staining reveals structural changes in the liver, while immunohistochemistry demonstrates reduced Ash2l expression in KO livers. Furthermore, levels of H3K4me3, a mark associated with gene activation, are decreased in Ash2l KO livers, implying a broader impact on epigenetic regulation. Cell proliferation assays using Ki-67 staining show a trend of reduced proliferation in Ash2l-deficient livers over time, particularly at 17 and 23 days post-induction. Modified Gomori's trichrome staining highlights potential fibrotic changes, and Periodic Acid-Schiff (PAS) staining indicates alterations in glycogen storage. Despite the observed biochemical and histological changes, the study did not find a statistically significant difference in total bilirubin (TBIL) between control and KO groups. Genetic analysis confirms successful generation of the Ash2l knockout model using Cre-loxP recombination. Overall, these findings suggest that Ash2l plays a crucial role in maintaining liver homeostasis and normal cell proliferation through its involvement in epigenetic regulation, and its deficiency leads to progressive liver dysfunction.",{"@graph":63,"@context":118},[64,80,101],{"@type":65,"itemListElement":66},"BreadcrumbList",[67,71,74,77],{"item":68,"name":69,"@type":70,"position":9},"https://docshare.wps.com","Home","ListItem",{"item":72,"name":10,"@type":70,"position":73},"https://docshare.wps.com/template/",2,{"item":75,"name":51,"@type":70,"position":76},"https://docshare.wps.com/template/general/",3,{"item":78,"name":59,"@type":70,"position":79},"https://docshare.wps.com/template/ash2l-deficient-livers-exhibit-altered-gene-expression-and-impaired-proliferation/191870/",4,{"url":78,"name":59,"@type":81,"image":82,"author":87,"headline":59,"publisher":90,"fileFormat":93,"inLanguage":57,"description":61,"dateModified":94,"datePublished":95,"encodingFormat":93,"isAccessibleForFree":96,"interactionStatistic":97},"DigitalDocument",{"url":83,"@type":84,"width":85,"height":86},"https://docshare.wps.com/thumbnails/ash2l-deficient-livers-exhibit-altered-gene-expression-and-impaired-proliferation/191870.png","ImageObject",442,249,{"name":88,"@type":89},"Emma Mercer","Person",{"url":68,"name":91,"@type":92},"DocShare","Organization","application/pdf","2026-10-01","2026-09-03",true,{"@type":98,"interactionType":99,"userInteractionCount":47},"InteractionCounter",{"@type":100},"ViewAction",{"@type":102,"mainEntity":103},"FAQPage",[104,110,114],{"name":105,"@type":106,"acceptedAnswer":107},"What are the key biochemical changes observed in Ash2l knockout livers?","Question",{"text":108,"@type":109},"Ash2l knockout livers show significantly elevated levels of alkaline phosphatase (ALP), alanine aminotransferase (ALT), and aspartate aminotransferase (AST), indicating liver damage and dysfunction.","Answer",{"name":111,"@type":106,"acceptedAnswer":112},"How does Ash2l deficiency impact epigenetic modification and gene expression?",{"text":113,"@type":109},"Loss of Ash2l leads to decreased levels of H3K4me3, a key epigenetic mark associated with gene activation, suggesting a disruption in gene expression regulation.",{"name":115,"@type":106,"acceptedAnswer":116},"What is the effect of Ash2l deficiency on liver cell proliferation?",{"text":117,"@type":109},"Ash2l deficiency impairs liver cell proliferation, as evidenced by a trend of reduced Ki-67 positive cells over time in knockout livers compared to controls.","https://schema.org",{"og:url":78,"og:type":120,"og:title":59,"og:site_name":91,"og:description":61},"article",{"robots":122,"canonical":78},"index,follow",{"doc_id":124,"site_id":56},191870,1788411388,{"code":4,"msg":5,"data":127},{"doc_id":124,"user_id":128,"nickname":88,"user_avatar":129,"doc_module":9,"category_id":50,"category_name":51,"doc_title":59,"doc_description":61,"doc_content":60,"file_id":130,"file_url":131,"file_type":132,"file_size":133,"view_count":47,"is_deleted":4,"is_public":9,"is_downloadable":9,"audit_status":9,"page_count":134,"language":135,"language_code":57,"site_id":56,"html_lang":57,"table_of_contents":136,"faqs":137,"seo_title":138,"seo_description":61,"update_tm":125,"read_time":76},962084925502,"https://ap-avatar.wpscdn.com/davatar_6f874abed73319feea01a86fa6f0fab8","cbCaivcGqzuid3xm","https://ap.wps.com/l/cbCaivcGqzuid3xm","pdf",881331,9,"English","# Introduction\n# Methods\n## Generation of Ash2l Conditional Knockout Mice\n## Biochemical Analysis of Liver Enzymes\n## Histological and Immunohistochemical Analysis\n## Assessment of Cell Proliferation\n# Results\n## Ash2l Deficiency Induces Liver Damage and Altered Enzyme Levels\n## Ash2l Loss Affects Epigenetic Marks and Gene Expression\n## Ash2l Deficiency Impairs Liver Cell Proliferation\n# Discussion","[{\"question\":\"What are the key biochemical changes observed in Ash2l knockout livers?\",\"answer\":\"Ash2l knockout livers show significantly elevated levels of alkaline phosphatase (ALP), alanine aminotransferase (ALT), and aspartate aminotransferase (AST), indicating liver damage and dysfunction.\"},{\"question\":\"How does Ash2l deficiency impact epigenetic modification and gene expression?\",\"answer\":\"Loss of Ash2l leads to decreased levels of H3K4me3, a key epigenetic mark associated with gene activation, suggesting a disruption in gene expression regulation.\"},{\"question\":\"What is the effect of Ash2l deficiency on liver cell proliferation?\",\"answer\":\"Ash2l deficiency impairs liver cell proliferation, as evidenced by a trend of reduced Ki-67 positive cells over time in knockout livers compared to controls.\"}]","ASH2L-Deficient Livers Exhibit Altered Gene Expression and Impaired Proliferation | PDF"]