[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"detail-sidebar-cat-1-en-105":3,"doc-detail-219320-en":53,"doc-seo-219320-105":76},{"code":4,"msg":5,"data":6},0,"success",[7,14,19,24,29,34,39,44,49],{"id":8,"doc_module":9,"doc_module_name":10,"category_name":11,"show_sort_weight":12,"slug":13},11,1,"Template","Presentations",90,"presentations",{"id":15,"doc_module":9,"doc_module_name":10,"category_name":16,"show_sort_weight":17,"slug":18},12,"Resumes",80,"resumes",{"id":20,"doc_module":9,"doc_module_name":10,"category_name":21,"show_sort_weight":22,"slug":23},14,"Invoices",70,"invoices",{"id":25,"doc_module":9,"doc_module_name":10,"category_name":26,"show_sort_weight":27,"slug":28},15,"Posters",60,"posters",{"id":30,"doc_module":9,"doc_module_name":10,"category_name":31,"show_sort_weight":32,"slug":33},16,"Social Media",50,"social-media",{"id":35,"doc_module":9,"doc_module_name":10,"category_name":36,"show_sort_weight":37,"slug":38},17,"Forms",40,"forms",{"id":40,"doc_module":9,"doc_module_name":10,"category_name":41,"show_sort_weight":42,"slug":43},18,"Letters",30,"letters",{"id":45,"doc_module":9,"doc_module_name":10,"category_name":46,"show_sort_weight":47,"slug":48},21,"Paper Templates",5,"papers-templates",{"id":50,"doc_module":9,"doc_module_name":10,"category_name":51,"show_sort_weight":4,"slug":52},158,"General","general-158",{"code":4,"msg":5,"data":54},{"doc_id":55,"user_id":56,"nickname":57,"user_avatar":58,"doc_module":9,"category_id":50,"category_name":51,"doc_title":59,"doc_description":60,"doc_content":61,"file_id":62,"file_url":63,"file_type":64,"file_size":65,"view_count":66,"is_deleted":4,"is_public":9,"is_downloadable":9,"audit_status":9,"page_count":67,"language":68,"language_code":69,"site_id":70,"html_lang":69,"table_of_contents":71,"faqs":72,"seo_title":73,"seo_description":60,"update_tm":74,"read_time":75},219320,687207412472,"Angel","https://ap-avatar.wpscdn.com/davatar_155a257f0dc6eb9ab79c44ca47cae57d","2022.012P.A-Tymoviridae 3nsp","This proposal describes three novel viruses from naranjilla and sorghum that are related yet distinct from existing members of the family Tymoviridae. It requests the creation of three new species, two within the genus Tymovirus and one within the genus Marafivirus. Species demarcation is supported using established genus criteria, including overall genome nucleotide identity below 80%, capsid protein sequence identity below 90%, differential host range, divergence in 3’-terminal structure, and serological specificity.","Part 1: TITLE, AUTHORS, APPROVALS, etc\nCode assigned:\n2022.012P\nShort title: Create two new species in the genus Tymovirus and one new species in the genus Marafivirus (Tymovirales:Tymoviridae)\nAuthor(s) and email address(es)\nHammond, RW (chair), Abrahamian P, Haenni A-L, Mollov D, Nagata T,\nSabanadzovic S\nRose.hammond@usda.gov; peter.abrahamian@usda.gov; anne-lise.haenni@ijm.fr; Dimitre.mollov@usda.gov; tatsuya@unb.br; ssabanadzovic@entomology.msstate.edu\nAuthor(s) institutional address(es) (optional)\nUSDA [RWH, PA, DM],\nUniversity of Brazil [TN],\nInstitut Jacques Monod emeritus [A-LH],\nMississippi State University [SS]\nCorresponding author\nRosemarie Hammond, rose.hammond@usda.gov\nList the ICTV Study Group(s) that have seen this proposal\nTymoviridae Study Group, Plant Viruses Subcommittee\nICTV Study Group comments and response of proposer\nICTV Study Group votes on proposal\nStudy Group\nNumber of members\nVotes support\nVotes against\nNo vote\nTymoviridae\n4\n2\nAuthority to use the name of a living person\nIs any taxon name used here derived from that of a living person (Y/N)\nTaxon name\nPerson from whom the name is derived\nPermission attached (Y/N)\nSubmission dates\nDate first submitted to SC Chair\nMay 26, 2022\nDate of this revision (if different to above)\nICTV-EC comments and response of the proposer\n\u000f\nPart 3: TAXONOMIC PROPOSAL\nName of accompanying Excel module\n2022.012P.A.v2.Tymoviridae_3nsp.xlxs\nAbstract\nThis proposal describes three novel viruses from naranjilla and sorghum that are related but distinct from current members of the family Tymoviridae.  Accordingly, we propose creation of three new species, two in the genus Tymovirus and one in the genus Marafivirus, to classify these viruses.\nText of proposal\nProposal to create 2 new species in the genus Tymovirus and 1 new species in the genus Marafivirus\nA. Currently applied criteria for species demarcation in the genus Tymovirus (Dreher et al., 2012) re:\nOverall genome sequence identity of less than 80%.\nCapsid protein sequences less than 90% identical.\nDifferential host range.\nDifferences in the 3′-terminal structure.\nSerological specificity.\n1. Naranjilla chlorotic mosaic virus (NarCMV; Tymovirus; Green et al., 2018; MG323924)\nNaranjilla chlorotic mosaic virus (NarCMV) was isolated and characterized by Green et al. (2018) from naranjilla (Solanum quitoense Lam.), a perennial shrub species cultivated in subtropical areas of Ecuador and Colombia. In 2015, a plant exhibiting symptoms of stunting, mosaic and chlorotic spots was sampled from the Pastaza province of Ecuador and spherical particles, composed of a single 20 kDa capsid protein, were observed under electron microscopy upon mechanical transmission to Nicotiana benthamiana indicator plants. High-throughput sequencing (HTS) was conducted from nucleic acids extracted from the indicator plant, and a single sequence contig was obtained that shared close relationships with tymoviruses. The complete 6,245 nucleotide sequence of the genome (MG323924) revealed a genome organization of three open reading frames (ORFs), a conserved tymobox typical of tymoviruses, a 3’ terminal 150 nt segment with a t-RNA-like secondary structure, and shared 71% sequence identity with the closest tymovirus, eggplant mosaic virus (EMV; KJ690172). This is below the 80% threshold for species demarcation in the genus Tymovirus in the family Tymoviridae (Dreher et al., 2012). The longest ORF encodes a 200 kDa protein containing replication-related domains, which shares 73% aa sequence identity with its closest relative, EMV. A smaller ORF which almost completely overlaps the 200 kDa protein, encodes a 69 kDa protein, and the 3’ proximal ORF, encodes the 188 aa coat protein (CP) of 20 kDa. The amino acid sequence of the CP was 83% identity to its closest match, chiltepin yellow mosaic virus (ChiYMV; FN563124), below the 90% threshold for species demarcation in the Tymoviridae Dreher et al., 2012). Phylogenetic analysis of the replicase and CP amino acids ","cbCaiboNyUVef47d","https://ap.wps.com/l/cbCaiboNyUVef47d","docx",751626,3,10,"English","en",105,"# Part 1: Title, Authors, Approvals, etc\n# Part 3: Taxonomic Proposal\n## Abstract\n## Text of proposal\n### Criteria for species demarcation in Tymovirus\n### 1. Naranjilla chlorotic mosaic virus (NarCMV)\n### 2. Naranjilla mild mosaic virus (NarMMV)","[{\"question\":\"What new species are proposed in this document?\",\"answer\":\"The proposal requests creation of three new species: two in the genus Tymovirus and one in the genus Marafivirus.\"},{\"question\":\"Which criteria are used to demarcate species within the genus Tymovirus?\",\"answer\":\"The document applies criteria including overall genome sequence identity below 80%, capsid protein identity below 90%, differential host range, differences in the 3′-terminal structure, and serological specificity.\"},{\"question\":\"What evidence supports NarCMV as a distinct Tymovirus species?\",\"answer\":\"NarCMV has complete genome identity below the 80% threshold for nucleotide demarcation, capsid protein identity below the 90% threshold, and a differential host range compared with the closest relatives.\"}]","2022.012P.A-Tymoviridae 3nsp | DOCX",1788864905,4,{"code":4,"msg":77,"data":78},"ok",{"site_id":70,"language":69,"slug":79,"title":59,"keywords":80,"description":60,"schema_data":81,"social_meta":135,"head_meta":137,"extra_data":139,"updated_unix":74},"2022012pa-tymoviridae-3nsp","",{"@graph":82,"@context":134},[83,97,117],{"@type":84,"itemListElement":85},"BreadcrumbList",[86,90,93,95],{"item":87,"name":88,"@type":89,"position":9},"https://docshare.wps.com","Home","ListItem",{"item":91,"name":10,"@type":89,"position":92},"https://docshare.wps.com/template/",2,{"item":94,"name":51,"@type":89,"position":66},"https://docshare.wps.com/template/general/",{"item":96,"name":59,"@type":89,"position":75},"https://docshare.wps.com/template/2022012pa-tymoviridae-3nsp/219320/",{"url":96,"name":59,"@type":98,"image":99,"author":104,"headline":59,"publisher":106,"fileFormat":109,"inLanguage":69,"description":60,"dateModified":110,"datePublished":111,"encodingFormat":109,"isAccessibleForFree":112,"interactionStatistic":113},"DigitalDocument",{"url":100,"@type":101,"width":102,"height":103},"https://docshare.wps.com/thumbnails/2022012pa-tymoviridae-3nsp/219320.png","ImageObject",442,249,{"name":57,"@type":105},"Person",{"url":87,"name":107,"@type":108},"DocShare","Organization","application/vnd.openxmlformats-officedocument.wordprocessingml.document","2026-09-20","2026-09-08",true,{"@type":114,"interactionType":115,"userInteractionCount":66},"InteractionCounter",{"@type":116},"ViewAction",{"@type":118,"mainEntity":119},"FAQPage",[120,126,130],{"name":121,"@type":122,"acceptedAnswer":123},"What new species are proposed in this document?","Question",{"text":124,"@type":125},"The proposal requests creation of three new species: two in the genus Tymovirus and one in the genus Marafivirus.","Answer",{"name":127,"@type":122,"acceptedAnswer":128},"Which criteria are used to demarcate species within the genus Tymovirus?",{"text":129,"@type":125},"The document applies criteria including overall genome sequence identity below 80%, capsid protein identity below 90%, differential host range, differences in the 3′-terminal structure, and serological specificity.",{"name":131,"@type":122,"acceptedAnswer":132},"What evidence supports NarCMV as a distinct Tymovirus species?",{"text":133,"@type":125},"NarCMV has complete genome identity below the 80% threshold for nucleotide demarcation, capsid protein identity below the 90% threshold, and a differential host range compared with the closest relatives.","https://schema.org",{"og:url":96,"og:type":136,"og:title":59,"og:site_name":107,"og:description":60},"article",{"robots":138,"canonical":96},"index,follow",{"doc_id":55,"site_id":70}]