[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"detail-sidebar-cat-0-en-105":3,"doc-seo-456684-105":59,"doc-detail-456684-en":130},{"code":4,"msg":5,"data":6},0,"success",[7,13,18,23,28,33,38,43,48,51,55],{"id":8,"doc_module":4,"doc_module_name":9,"category_name":10,"show_sort_weight":11,"slug":12},1,"Document","Story & Novel",90,"story-novel",{"id":14,"doc_module":4,"doc_module_name":9,"category_name":15,"show_sort_weight":16,"slug":17},2,"Literature",80,"literature",{"id":19,"doc_module":4,"doc_module_name":9,"category_name":20,"show_sort_weight":21,"slug":22},4,"Exam",70,"exam",{"id":24,"doc_module":4,"doc_module_name":9,"category_name":25,"show_sort_weight":26,"slug":27},5,"Comic",60,"comic",{"id":29,"doc_module":4,"doc_module_name":9,"category_name":30,"show_sort_weight":31,"slug":32},6,"Technology",50,"technology",{"id":34,"doc_module":4,"doc_module_name":9,"category_name":35,"show_sort_weight":36,"slug":37},7,"Healthcare",40,"healthcare",{"id":39,"doc_module":4,"doc_module_name":9,"category_name":40,"show_sort_weight":41,"slug":42},8,"Research & Report",30,"research-report",{"id":44,"doc_module":4,"doc_module_name":9,"category_name":45,"show_sort_weight":46,"slug":47},9,"Religion & Spirituality",20,"religion-spirituality",{"id":46,"doc_module":4,"doc_module_name":9,"category_name":49,"show_sort_weight":46,"slug":50},"World Cup","world-cup",{"id":52,"doc_module":4,"doc_module_name":9,"category_name":53,"show_sort_weight":52,"slug":54},10,"Lifestyle","lifestyle",{"id":56,"doc_module":4,"doc_module_name":9,"category_name":57,"show_sort_weight":24,"slug":58},19,"General","general",{"code":4,"msg":60,"data":61},"ok",{"site_id":62,"language":63,"slug":64,"title":65,"keywords":66,"description":67,"schema_data":68,"social_meta":123,"head_meta":125,"extra_data":127,"updated_unix":129},105,"en","zdhhc14-enhances-p16-stability-via-palmitoylation-to-inhibit-prostate-cancer-progression","ZDHHC14 enhances P16 stability via palmitoylation to inhibit prostate cancer progression","","ZDHHC14 enhances the stability of the tumor suppressor P16 (CDKN2A) by promoting palmitoylation at the Cys 72 residue, thereby suppressing ubiquitination-dependent degradation. CDKN2A overexpression significantly inhibited prostate cancer cell proliferation, migration, and invasion, while pharmacologic modulation of palmitoylation and C72S mutation clarified the causal role of this modification. ZDHHC14 knockdown reduced P16 protein levels and confirmed direct interaction, linking the ZDHHC14/P16 axis to antitumor behavior.",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":40,"@type":76,"position":81},"https://docshare.wps.com/document/research-report/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/zdhhc14-enhances-p16-stability-via-palmitoylation-to-inhibit-prostate-cancer-progression/456684/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/zdhhc14-enhances-p16-stability-via-palmitoylation-to-inhibit-prostate-cancer-progression/456684.png","ImageObject",300,407,{"name":92,"@type":93},"Jiven","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-10-07","2026-09-30",true,{"@type":102,"interactionType":103,"userInteractionCount":14},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"What role does P16 play in prostate cancer in this study?","Question",{"text":112,"@type":113},"P16 (CDKN2A) acts as a tumor suppressor, and its overexpression inhibits proliferation, migration, and invasion of prostate cancer cells.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"How does palmitoylation affect P16 stability?",{"text":117,"@type":113},"Palmitoylation of P16 at Cys 72 enhances P16 stability by suppressing ubiquitination-dependent degradation.",{"name":119,"@type":110,"acceptedAnswer":120},"What is the specific function of ZDHHC14?",{"text":121,"@type":113},"ZDHHC14 was identified as the palmitoyltransferase that modifies P16, and ZDHHC14 knockdown decreases P16 protein levels, supporting a direct interaction driving the palmitoylation process.","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},456684,1791336893,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":39,"category_name":40,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":14,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":139,"language":140,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":141,"faqs":142,"seo_title":143,"seo_description":67,"update_tm":144,"read_time":145},1099513958607,"https://ap-avatar.wpscdn.com/avatar/100002390cf8733938c?x-image-process=image/resize,m_fixed,w_180,h_180&k=1778829742770036399","ZDHHC14 enhances P16 stability via palmitoylation to inhibit prostate cancer progression  \nJian Zhao1\\#, Gang Ni2\\#, Zhichao Wu1, Chunhong Zhang1  \n1Department of Urology, Taihe Traditional Chinese Medical Hospital Affiliated to Anhui University of Chinese Medicine, Fuyang, China; 2Reproductive Medicine Centre, Maternal and Child Health Hospital, Hefei, China  \nContributions: (I) Conception and design: J Zhao, G Ni; (II) Administrative support: C Zhang; (III) Provision of study materials or patients: J Zhao, GNi; (IV) Collection and assembly of data: J Zhao, G Ni; (V) Data analysis and interpretation: J Zhao, G Ni; (VI) Manuscript writing: All authors; (VII) Final approval of manuscript: All authors.  \n\\#These authors contributed equally to this work as co-first authors.  \nCorrespondence to: Chunhong Zhang, MM. Department of Urology, Taihe Traditional Chinese Medical Hospital Affiliated to Anhui University of  \nChinese Medicine, No. 59, Tuanjie West Road, Fuyang 236600, China. Email: [362969345@qq.com](362969345@qq.com).  \nBackground: The progression of prostate cancer is closely associated with dysregulation of tumor suppressor genes. P16 (CDKN2A), a key cell cycle regulator, is frequently downregulated in malignant phenotypes, yet the role of its post-translational modifications, particularly palmitoylation, remains unclear.  \nThis study aimed to investigate the regulatory mechanism of palmitoylation on P16 stability and explore the functional role of ZDHHC14 in this process, as well as its impact on the malignant behaviors of prostate  \ncancer cells.  \nMethods: CDKN2A was overexpressed in prostate cancer cells (PC-3 and DU-145) via plasmid transfection.  \nThe inhibitory effects of P16 on proliferation, migration, and invasion were evaluated using quantitative real-time polymerase chain reaction (qRT-PCR), Western blot, colony formation assay, and Transwell assays. The palmitoylation modification site (Cys 72) and its regulatory role in P16 stability were analyzed through palmitoylation inhibitor [2-bromopalmitate (2-BP)] and agonist [hexadecyl acetyl phosphate (HAM)] treatments, site-directed mutagenesis (C72S), ubiquitination assays, and molecular docking. Bioinformatics screening, small interfering RNA (siRNA) knockdown, and co-immunoprecipitation (Co-IP) were employed  \nto validate the interaction between ZDHHC14 and P16 and their functional relationship.  \nResults: Overexpression of CDKN2A significantly inhibited the proliferation, migration, and invasion of prostate cancer cells. Palmitoylation at the Cys 72 site enhanced P16 stability by suppressing its ubiquitination-dependent degradation. ZDHHC14 was identified as the palmitoyltransferase responsible for modifying P16, and its expression positively correlated with patient survival. Knockdown of ZDHHC14 markedly reduced P16 protein levels, and their direct interaction was confirmed to mediate the  \npalmitoylation process.  \nConclusions: This study reveals for the first time that ZDHHC14 enhances P16 stability and suppresses its ubiquitination-mediated degradation via palmitoylation at the Cys 72 residue, thereby exerting antitumor effects. These findings provide a theoretical foundation for developing therapeutic strategies targeting the  \nZDHHC14/P16 axis in prostate cancer.  \nKeywords: Prostate cancer; ZDHHC14; P16; palmitoylation  \nSubmitted Aug 04, 2025. Accepted for publication Nov 04, 2025. Published online Dec 24, 2025.  \ndoi: 10.21037/tau-2025-552  \nView this article at: [https://dx.doi.org/10.21037/tau-2025-552](https://dx.doi.org/10.21037/tau-2025-552)  \n© AME Publishing Company. Transl Androl Urol 2025;14(12):3806-3816 | [https://dx.doi.org/10.21037/tau-2025-552](https://dx.doi.org/10.21037/tau-2025-552)  \nTranslational Andrology and Urology, Vol 14, No 12 December 2025 3807  \nIntroduction  \nProstate cancer is one of the most common malignant tumors in men worldwide, and its progression and metastasis pose significant challenges to clinical treatment (1) .","cbCaij5e6VWthgo6","https://ap.wps.com/l/cbCaij5e6VWthgo6","pdf",3076848,11,"English","# Introduction\n# Methods\n# Results\n# Conclusions\n# Keywords\n# Highlight box\n## Key findings\n## What is known and what is new?\n## What is the implication, and what should change now?","[{\"question\":\"What role does P16 play in prostate cancer in this study?\",\"answer\":\"P16 (CDKN2A) acts as a tumor suppressor, and its overexpression inhibits proliferation, migration, and invasion of prostate cancer cells.\"},{\"question\":\"How does palmitoylation affect P16 stability?\",\"answer\":\"Palmitoylation of P16 at Cys 72 enhances P16 stability by suppressing ubiquitination-dependent degradation.\"},{\"question\":\"What is the specific function of ZDHHC14?\",\"answer\":\"ZDHHC14 was identified as the palmitoyltransferase that modifies P16, and ZDHHC14 knockdown decreases P16 protein levels, supporting a direct interaction driving the palmitoylation process.\"}]","ZDHHC14 enhances P16 stability via palmitoylation to inhibit prostate cancer progression | PDF",1790746715,28]