[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"detail-sidebar-cat-0-en-105":3,"doc-seo-420820-105":59,"doc-detail-420820-en":130},{"code":4,"msg":5,"data":6},0,"success",[7,13,18,23,28,33,38,43,48,51,55],{"id":8,"doc_module":4,"doc_module_name":9,"category_name":10,"show_sort_weight":11,"slug":12},1,"Document","Story & Novel",90,"story-novel",{"id":14,"doc_module":4,"doc_module_name":9,"category_name":15,"show_sort_weight":16,"slug":17},2,"Literature",80,"literature",{"id":19,"doc_module":4,"doc_module_name":9,"category_name":20,"show_sort_weight":21,"slug":22},4,"Exam",70,"exam",{"id":24,"doc_module":4,"doc_module_name":9,"category_name":25,"show_sort_weight":26,"slug":27},5,"Comic",60,"comic",{"id":29,"doc_module":4,"doc_module_name":9,"category_name":30,"show_sort_weight":31,"slug":32},6,"Technology",50,"technology",{"id":34,"doc_module":4,"doc_module_name":9,"category_name":35,"show_sort_weight":36,"slug":37},7,"Healthcare",40,"healthcare",{"id":39,"doc_module":4,"doc_module_name":9,"category_name":40,"show_sort_weight":41,"slug":42},8,"Research & Report",30,"research-report",{"id":44,"doc_module":4,"doc_module_name":9,"category_name":45,"show_sort_weight":46,"slug":47},9,"Religion & Spirituality",20,"religion-spirituality",{"id":46,"doc_module":4,"doc_module_name":9,"category_name":49,"show_sort_weight":46,"slug":50},"World Cup","world-cup",{"id":52,"doc_module":4,"doc_module_name":9,"category_name":53,"show_sort_weight":52,"slug":54},10,"Lifestyle","lifestyle",{"id":56,"doc_module":4,"doc_module_name":9,"category_name":57,"show_sort_weight":24,"slug":58},19,"General","general",{"code":4,"msg":60,"data":61},"ok",{"site_id":62,"language":63,"slug":64,"title":65,"keywords":66,"description":67,"schema_data":68,"social_meta":123,"head_meta":125,"extra_data":127,"updated_unix":129},105,"en","you-bacteria-shall-not-pass-nlrp6-can-sense-you-news-views-nlrp6-detection-role-in-bacterial-spread","You (bacteria) shall not pass: NLRP6 can sense you! - News & Views - NLRP6 detection role in bacterial spread","","Intracellular bacteria can spread directly from one host cell to the next, bypassing extracellular immune defenses and thereby worsening infection outcomes. In this News & Views, Boegli and colleagues reveal that the innate immune sensor NLRP6 detects bacteria-induced lysosomal damage and triggers cytokine release or cell death. The article explains how cell-to-cell transmission evades extracellular mediators, then describes NLRP6’s inflammasome assembly, caspase-1 activation, and pyroptotic gasdermin cleavage to both warn the host and disrupt bacterial hiding.",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":35,"@type":76,"position":81},"https://docshare.wps.com/document/healthcare/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/you-bacteria-shall-not-pass-nlrp6-can-sense-you-news-views-nlrp6-detection-role-in-bacterial-spread/420820/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/you-bacteria-shall-not-pass-nlrp6-can-sense-you-news-views-nlrp6-detection-role-in-bacterial-spread/420820.png","ImageObject",300,407,{"name":92,"@type":93},"Olivia Brown","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-10-31","2026-09-28",true,{"@type":102,"interactionType":103,"userInteractionCount":44},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"Why is cell-to-cell spread by intracellular bacteria important for infection progression?","Question",{"text":112,"@type":113},"It allows bacteria to move directly between host cells while avoiding extracellular immune defenses and drug exposure. This helps sustain dissemination and increases infection severity.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"What new role of NLRP6 is highlighted in this issue?",{"text":117,"@type":113},"NLRP6 is described as sensing bacteria-induced lysosomal damage and then triggering cytokine release or cell death to limit infection.",{"name":119,"@type":110,"acceptedAnswer":120},"How does NLRP6 drive the immune outcome once it detects the relevant signal?",{"text":121,"@type":113},"NLRP6 assembles an inflammasome that links the sensor to caspase-1, enabling maturation and release of inflammatory cytokines and cleavage of gasdermin proteins to execute pyroptotic cell death.","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},420820,1791171863,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":34,"category_name":35,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":44,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":81,"language":139,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":140,"faqs":141,"seo_title":142,"seo_description":67,"update_tm":143,"read_time":39,"preview_page_count":4},16904993612988,"https://ap-avatar.wpscdn.com/davatar_a8503ba1806abce46bf441b54a3ca4cd","News & Views  \nYou (bacteria) shall not pass: NLRP6 can sense you!  \nAlexander N R Weber 1,2,3,4 ✉ & Philip Rosenstiel 5 ✉  \nThe ability of intracellular bacteria to spread directly from one cell to the next, avoiding extracellular immune defensesand drugs, is essential for pathogen dissemination and, ultimately, the progression and severity of infection. In this issue of The EMBO Journal, Boegli and colleagues uncover a previously unrecognized role of the enigmatic innate immune sensor NLRP6 in detecting bacteria-induced lysosomal damage and triggering cytokine release or cell death to limit infection.  \nSee also: A Boegli et al  \nBacteria attempting to spread within a  \nmulticellular host face multiple chal  \nlenges as they seek out protected niches that shield them from direct immune defense mechanisms. Extracellularly, they may be especially vulnerable to a whole arsenal of immune mediators, e.g., antimicrobial peptides, complement proteins, antibodies, or phagocytes. For invasive pathogens, including species of Listeria, Shigella, Orientia, Chlamydia, and Salmonella, a far safer route for spreading while avoiding the risks of the extracellular space, is direct cell-to-cell transmission from an infected host cell to its neighbor. For example, Listeria and Shigella propel themselves from the plasma membrane of an infected cell into the plasma membrane of an adjacent cell using the force generated by actin-based motility, and then become engulfed into a vacuole before escaping into the cytosol of the neighboring cell. However, the host has evolved an additional layer of defense involving a distinct family of cytosolic pattern-recognition receptors (PRRs) known as Nod-like receptors  \n(NLRs) . These intracellular sentinels can recognize specific microbe- or pathogenassociated molecular patterns (MAMPs or PAMPs, respectively), such as bacterial cellwall components (e.g., muramyl dipeptide or flagellin), once these signals cross the membrane barrier and enter the cytosol. An alternative strategy of danger sensing by NLRs is the detection of cellular stress signals that reflect perturbations in homeostasis, e.g., potassium efflux, ROS, or ATP levels.  \nAlthough the family member NLRP6 had also been proposed to sense infections through recognition of specific MAMPs, Boegli et al now suggest that NLRP6 sensesan infection-relevant process rather than a microbe-specific molecule (Fig. 1) . Specifi cally, they show that NLRP6 can function asan intracellular “trip-wire” for incoming bacteria seeking to enter via a neighboring cell. It does so by assembling a so-called inflammasome, a multiprotein complex that bridges the sensor, NLRP6, to an enzyme, caspase-1, that is able to mature inflammatory cytokines (e.g., IL-1β or IL-18) for subsequent release, and to cleave gasdermin (GSDM) family proteins to execute pyroptotic, i.e., inflammatory, cell death. The effect is twofold: raising an alarm to infection and shutting down the bacterial hiding place.  \nThe description of NLRP6 as a sensor for cell-to-cell spread was unexpected. NLRP6 (initially named PYPAF5) was first proposed not to act as a bona fide PRR but rather as a transcriptional regulator of other PRRs (Grenier et al, 2002) and a negative regulator of inflammatory signaling during bacterial pathogen clearance, including Listeria spp. (Anand et al, 2012) . Only  \nsubsequently, NLRP6 was suggested to directly respond to certain metabolites (e.g., taurine, Levy et al, 2015) or a MAMP, namely double-stranded RNA from intestinal viruses (Wang et al, 2015), in intestinal epithelial cells (IECs), where it is most prominently expressed in both humans and mice. However, direct ligand binding and the underlying molecular mechanisms remained unclear until more recently, when RNA was shown to bind NLRP6 in vitro and trigger phase-separation as a potential way of bringing together a sufficient number of NLRP6 molecules to form an inflammasome (Shen et al, 2021) . Apart from IECs (Levy et al, 2015), a role","cbCaibljOgtBRvDl","https://ap.wps.com/l/cbCaibljOgtBRvDl","pdf",529571,"English","# News & Views\n## NLRP6 senses lysosomal damage and triggers immune responses\n## Cell-to-cell spread and intracellular danger sensing by NLRs\n## Past debates and multifaceted NLRP6 sensing capabilities","[{\"question\":\"Why is cell-to-cell spread by intracellular bacteria important for infection progression?\",\"answer\":\"It allows bacteria to move directly between host cells while avoiding extracellular immune defenses and drug exposure. This helps sustain dissemination and increases infection severity.\"},{\"question\":\"What new role of NLRP6 is highlighted in this issue?\",\"answer\":\"NLRP6 is described as sensing bacteria-induced lysosomal damage and then triggering cytokine release or cell death to limit infection.\"},{\"question\":\"How does NLRP6 drive the immune outcome once it detects the relevant signal?\",\"answer\":\"NLRP6 assembles an inflammasome that links the sensor to caspase-1, enabling maturation and release of inflammatory cytokines and cleavage of gasdermin proteins to execute pyroptotic cell death.\"}]","You (bacteria) shall not pass: NLRP6 can sense you! - News & Views - NLRP6 detection role in bacterial spread | PDF",1790622530]