[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"detail-sidebar-cat-0-en-105":3,"doc-seo-431327-105":59,"doc-detail-431327-en":130},{"code":4,"msg":5,"data":6},0,"success",[7,13,18,23,28,33,38,43,48,51,55],{"id":8,"doc_module":4,"doc_module_name":9,"category_name":10,"show_sort_weight":11,"slug":12},1,"Document","Story & Novel",90,"story-novel",{"id":14,"doc_module":4,"doc_module_name":9,"category_name":15,"show_sort_weight":16,"slug":17},2,"Literature",80,"literature",{"id":19,"doc_module":4,"doc_module_name":9,"category_name":20,"show_sort_weight":21,"slug":22},4,"Exam",70,"exam",{"id":24,"doc_module":4,"doc_module_name":9,"category_name":25,"show_sort_weight":26,"slug":27},5,"Comic",60,"comic",{"id":29,"doc_module":4,"doc_module_name":9,"category_name":30,"show_sort_weight":31,"slug":32},6,"Technology",50,"technology",{"id":34,"doc_module":4,"doc_module_name":9,"category_name":35,"show_sort_weight":36,"slug":37},7,"Healthcare",40,"healthcare",{"id":39,"doc_module":4,"doc_module_name":9,"category_name":40,"show_sort_weight":41,"slug":42},8,"Research & Report",30,"research-report",{"id":44,"doc_module":4,"doc_module_name":9,"category_name":45,"show_sort_weight":46,"slug":47},9,"Religion & Spirituality",20,"religion-spirituality",{"id":46,"doc_module":4,"doc_module_name":9,"category_name":49,"show_sort_weight":46,"slug":50},"World Cup","world-cup",{"id":52,"doc_module":4,"doc_module_name":9,"category_name":53,"show_sort_weight":52,"slug":54},10,"Lifestyle","lifestyle",{"id":56,"doc_module":4,"doc_module_name":9,"category_name":57,"show_sort_weight":24,"slug":58},19,"General","general",{"code":4,"msg":60,"data":61},"ok",{"site_id":62,"language":63,"slug":64,"title":65,"keywords":66,"description":67,"schema_data":68,"social_meta":123,"head_meta":125,"extra_data":127,"updated_unix":129},105,"en","well-to-well-transfer-of-halotag-ligand-6-chlorohexan-1-ol-and-formaldehyde-in-a-multi-well-plate","Well-to-well transfer of HaloTag ligand 6-chlorohexan-1-ol and formaldehyde in a multi-well plate","","Volatile organic compounds handled in multi-well plates can volatilize and impact wells that are not directly treated. Two case studies show that HaloTag ligand 6-chlorohexan-1-ol and formaldehyde can transfer between wells, producing unexpected experimental outcomes. HaloTag-targeting PROTAC activity is altered due to competition effects, and multifaceted analysis attributes the changes to well-to-well transfer of 6-chlorohexan-1-ol. Formaldehyde used for cell fixation also harms nearby wells, requiring careful reagent handling in multi-well workflows.",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":35,"@type":76,"position":81},"https://docshare.wps.com/document/healthcare/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/well-to-well-transfer-of-halotag-ligand-6-chlorohexan-1-ol-and-formaldehyde-in-a-multi-well-plate/431327/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/well-to-well-transfer-of-halotag-ligand-6-chlorohexan-1-ol-and-formaldehyde-in-a-multi-well-plate/431327.png","ImageObject",300,407,{"name":92,"@type":93},"นรินทร์","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-10-31","2026-09-29",true,{"@type":102,"interactionType":103,"userInteractionCount":34},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"What problem does the paper address in multi-well plates?","Question",{"text":112,"@type":113},"It addresses how volatile compounds can volatilize and then transfer to untreated wells, causing cross-well effects and unexpected anomalies in experiments.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"How does 6-chlorohexan-1-ol influence PROTAC-related results?",{"text":117,"@type":113},"The study finds that competition involving the HaloTag-targeting PROTAC and 6-chlorohexan-1-ol affects other wells, attributed to well-to-well transfer of 6-chlorohexan-1-ol into untreated wells.",{"name":119,"@type":110,"acceptedAnswer":120},"Why is formaldehyde highlighted as a concern?",{"text":121,"@type":113},"Formaldehyde used for cell fixation was found to be toxic to cells in surrounding wells, indicating it can also create harmful cross-well interference.","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},431327,1790827715,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":34,"category_name":35,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":34,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":139,"language":140,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":141,"faqs":142,"seo_title":143,"seo_description":67,"update_tm":144,"read_time":145,"preview_page_count":4},2336475104957,"https://ap-avatar.wpscdn.com/avatar/22000c4c6bd8a5076e1?x-image-process=image/resize,m_fixed,w_180,h_180&k=1787554080175789136","[www. nature.com/scientificreports](www. nature.com/scientificreports)  \nOPEN  \nWell-to-well transfer of HaloTag ligand 6-chlorohexan-1-ol and formaldehyde in a multi-well plate  \nYugo Mishima1􀀍, SaoriYasuda1, Aoi Kunitomi1, Tokiha Masuda-Ozawa1, Shusuke Tomoshige1, Shunji Kato2, Yuki Kadono2, Kiyotaka Nakagawa2 & Minoru Ishikawa1􀀍  \nWhen volatile organic compounds are handled in multi-well plates, their volatilization is known to affect non-treated wells in the plate. In this paper, we report two cases in which the HaloTag ligand 6-chlorohexan-1-ol and formaldehyde were volatilized and transferred to other wells in a multi-well plate. We found that competition between the HaloTag-targeting PROTAC and 6-chlorohexan-1-ol affected the other wells ofthe plate. A multifaceted analysis revealed that this phenomenon resulted from well-to-well transfer of 6-chlorohexan-1-ol into untreated wells on the plate. In addition, the formaldehyde used for cell fixation was toxic to cells in the surrounding wells. Therefore, even these reagents, which are widely used in life science research, require careful handling in multi-well plates.  \nKeywords Multi-well plate, Volatilization, HaloTag, 6-chlorohexan-1-ol, Formaldehyde  \nHaloTag is a tag protein that can form a covalent bond with chloroalkane1 (Fig. 1A), and is often used in life science research. Recently, HaloTag has been used in proof-of-concept studies for targeted protein degraders, such as proteolysis-targeting chimeras (PROTACs)2–5. PROTACs have a structure that links the ligand of ubiquitin ligase (E3) to the ligand of the target protein, bringing the two proteins into proximity and inducing ubiquitination and proteasomal degradation of the target protein6 (Fig. 1B) . Confirming that the degradation activity of PROTAC is canceled by competing with an excess amount of each protein ligand is important in analyzing the mode ofaction of the newly synthesized PROTAC. This competition assay is generally accepted in PROTAC research (Fig. 1C) .  \nMulti-well plates are frequently used in life science research involving proteins and cells, including PROTAC development. Each well is generally recognized as an independent system. However, some compounds have been reported to affect other wells ofthe plate through volatilization. For instance, Adolphe et al. found that ethanol and butanol volatilize and are transferred to other wells7. Novy et al. reported that plant-derived compounds, such as thymoquinone, can diffuse in a plate and cause false positives in assays and proposed the use of an ethylene vinyl acetate (EVA) capmat as a vapor barrier to avoid this problem8,9. Castell et al. also observed the cross-contamination of phenol derivatives in a multi-well plate and reported that the use of plastic seals could prevent contamination without adversely affecting cell integrity10. These results indicate that assays using multiwell plates require attention to the volatilization of test compounds. However, this phenomenon has not received much attention because the reported compounds are not universally used in biological research.  \nIn this study, we investigated the volatilization and transfer of 6-chlorohexan-1-ol between wells on multiwell plates. This compound is often used in life science research as a ligand for HaloTag, and its volatility is often underestimated owing to its high boiling point of 212.8 °C11. In addition, we tested the possibility that formaldehyde, which is used to fix cells and tissues12, could also affect the surrounding wells in a plate. If these commonly used compounds are transferred to other wells, researchers may encounter unexpected anomalies during the experiments. Therefore, we have shared this information in this study.  \n1Graduate School of Life Sciences, Tohoku University, 2-1-1 Katahira, Aoba-ku, Sendai, Miyagi 980-8577, Japan.  \n2Graduate School of Agricultural Science, Tohoku University, 468-1 Aramaki Aza Aoba, Aoba-ku, Sendai, Miyagi 980-8572, Japan. 􀀍 [","cbCaigenNOBOxusE","https://ap.wps.com/l/cbCaigenNOBOxusE","pdf",1998369,11,"English","# Results\n## HaloTag ligand 6-chlorohexan-1-ol-treated wells affect other wells in a multi-well plate","[{\"question\":\"What problem does the paper address in multi-well plates?\",\"answer\":\"It addresses how volatile compounds can volatilize and then transfer to untreated wells, causing cross-well effects and unexpected anomalies in experiments.\"},{\"question\":\"How does 6-chlorohexan-1-ol influence PROTAC-related results?\",\"answer\":\"The study finds that competition involving the HaloTag-targeting PROTAC and 6-chlorohexan-1-ol affects other wells, attributed to well-to-well transfer of 6-chlorohexan-1-ol into untreated wells.\"},{\"question\":\"Why is formaldehyde highlighted as a concern?\",\"answer\":\"Formaldehyde used for cell fixation was found to be toxic to cells in surrounding wells, indicating it can also create harmful cross-well interference.\"}]","Well-to-well transfer of HaloTag ligand 6-chlorohexan-1-ol and formaldehyde in a multi-well plate | PDF",1790655189,28]