[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"detail-sidebar-cat-0-en-105":3,"doc-seo-297504-105":59,"doc-detail-297504-en":130},{"code":4,"msg":5,"data":6},0,"success",[7,13,18,23,28,33,38,43,48,51,55],{"id":8,"doc_module":4,"doc_module_name":9,"category_name":10,"show_sort_weight":11,"slug":12},1,"Document","Story & Novel",90,"story-novel",{"id":14,"doc_module":4,"doc_module_name":9,"category_name":15,"show_sort_weight":16,"slug":17},2,"Literature",80,"literature",{"id":19,"doc_module":4,"doc_module_name":9,"category_name":20,"show_sort_weight":21,"slug":22},4,"Exam",70,"exam",{"id":24,"doc_module":4,"doc_module_name":9,"category_name":25,"show_sort_weight":26,"slug":27},5,"Comic",60,"comic",{"id":29,"doc_module":4,"doc_module_name":9,"category_name":30,"show_sort_weight":31,"slug":32},6,"Technology",50,"technology",{"id":34,"doc_module":4,"doc_module_name":9,"category_name":35,"show_sort_weight":36,"slug":37},7,"Healthcare",40,"healthcare",{"id":39,"doc_module":4,"doc_module_name":9,"category_name":40,"show_sort_weight":41,"slug":42},8,"Research & Report",30,"research-report",{"id":44,"doc_module":4,"doc_module_name":9,"category_name":45,"show_sort_weight":46,"slug":47},9,"Religion & Spirituality",20,"religion-spirituality",{"id":46,"doc_module":4,"doc_module_name":9,"category_name":49,"show_sort_weight":46,"slug":50},"World Cup","world-cup",{"id":52,"doc_module":4,"doc_module_name":9,"category_name":53,"show_sort_weight":52,"slug":54},10,"Lifestyle","lifestyle",{"id":56,"doc_module":4,"doc_module_name":9,"category_name":57,"show_sort_weight":24,"slug":58},19,"General","general",{"code":4,"msg":60,"data":61},"ok",{"site_id":62,"language":63,"slug":64,"title":65,"keywords":66,"description":67,"schema_data":68,"social_meta":123,"head_meta":125,"extra_data":127,"updated_unix":129},105,"en","w520_tryptic-soy-agar-tsa-media-usp-irradiated","W520_Tryptic Soy Agar (TSA) Media, USP, Irradiated","","Hardy Diagnostics Tryptic Soy Agar (TSA), USP, Irradiated is intended as a general growth medium for detecting and enumerating microorganisms in microbiological examinations of non-sterile products. The formulation follows USP \u003C62>, supporting harmonized standards for microbial testing. It includes casein and soybean meal digests to promote microbial growth, with sodium chloride for osmotic balance and lecithin plus Tween 80 to neutralize disinfectant or cleaning effects. Sterilization is achieved by irradiation, and the product is for laboratory use only, not for diagnosing human disease.",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":35,"@type":76,"position":81},"https://docshare.wps.com/document/healthcare/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/w520_tryptic-soy-agar-tsa-media-usp-irradiated/297504/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/w520_tryptic-soy-agar-tsa-media-usp-irradiated/297504.png","ImageObject",300,407,{"name":92,"@type":93},"8796093062539","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-10-22","2026-09-18",true,{"@type":102,"interactionType":103,"userInteractionCount":34},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"What is Tryptic Soy Agar (TSA), USP, Irradiated used for?","Question",{"text":112,"@type":113},"It is recommended as a general growth medium to detect and enumerate microorganisms in the microbiological examination of non-sterile products. It can also support establishing microbiological trends, alerts, and action levels in biologically controlled environments.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"What are the key ingredients and their roles in TSA?",{"text":117,"@type":113},"TSA contains digests of soybean meal and casein to provide amino acids and nitrogenous compounds for microbial growth. Sodium chloride maintains osmotic equilibrium, agar solidifies the medium, and lecithin plus Tween 80 help neutralize antimicrobial effects of disinfectants or cleaning solutions.",{"name":119,"@type":110,"acceptedAnswer":120},"How should the irradiated TSA be stored and handled for use?",{"text":121,"@type":113},"Store at 15–30°C away from direct light, protect from freezing, and do not use if contamination, deterioration, discoloration, or expired dating is present. Before use, warm to room temperature and ensure the surface is dry; if condensation is observed, remove plates in a sterile environment and allow them to dry for 10–15 minutes.","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},297504,1790077364,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":66,"doc_module":4,"category_id":34,"category_name":35,"doc_title":65,"doc_description":67,"doc_content":133,"file_id":134,"file_url":135,"file_type":136,"file_size":137,"view_count":34,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":29,"language":138,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":139,"faqs":140,"seo_title":141,"seo_description":67,"update_tm":142,"read_time":143},8796093062539,"TRYPTIC SOY AGAR (TSA) MEDIA, USP, IRRADIATED  \n\n| [Cat. no. P520](Cat. no. P520) | Tryptic Soy Agar (TSA), with Lecithin and Tween® 80, USP, Irradiated, Triple Bagged, Contact Plate, Lok-Tight™, 15ml* | 10 plates/bag |\n| --- | --- | --- |\n| [Cat. no. P520R](Cat. no. P520R) | Tryptic Soy Agar (TSA), with Lecithin and Tween® 80, USP, Irradiated, Triple Bagged, Red-Tinted Contact Plate, LokTight™, 15ml* | 10 plates/bag |\n| [Cat. no. W520](Cat. no. W520) | Tryptic Soy Agar (TSA) with Lecithin and Tween® 80, USP, SterEM™, Irradiated, Triple Bagged, 15x100mm Plate, 26ml* | 10 plates/bag |\n| [Cat. no. W540](Cat. no. W540) | Tryptic Soy Agar (TSA), USP, SterEM™, Irradiated, Triple Bagged, 15x100mm Plate, 34ml* | 10 plates/bag |\n| [Cat. no. W540R](Cat. no. W540R) | Tryptic Soy Agar, USP, SterEM™, Irradiated, Triple Bagged, Red-Tinted 15x100mm Plate, 34ml* | 10 plates/bag |\n| [Cat. no. W570](Cat. no. W570) | Tryptic Soy Agar (TSA), USP, SterEM™, Irradiated, Triple Bagged, 15x100mm Plate, 26ml* | 10 plates/bag |\n| [Cat. no. W570R](Cat. no. W570R) | Tryptic Soy Agar (TSA), USP, SterEM™, Irradiated, Triple Bagged, Red-Tinted 15x100mm Plate, 26ml* | 10 plates/bag |\n| * A fourth sterile sample bag is included for packaging after the sample is collected. |  |  |\n\nINTENDED USE  \nHardy Diagnostics Tryptic Soy Agar (TSA), USP, Irradiated is recommended for use as a general growth medium for the detection and enumeration of microorganisms in the microbiological examination of non-sterile products. Tryptic Soy Agar (TSA) with Lecithin and Tween®, USP, Irradiated is recommended for use as a general growth medium for establishing microbiological trends, alerts, and action levels in biologically controlled environments.  \nThe media are formulated according to the United States Pharmacopoeia (USP) \u003C62> and meet the harmonized USP/EP/JP standards for the microbial examination of non-sterile products.  \nThis product is not intended to be used for the diagnosis of human disease.  \nSUMMARY  \nThe formulation of Tryptic Soy Agar, USP is prepared according to the United States Pharmacopeia standard formula for Soybean-Casein Digest Agar Medium.(2)  \nHardy Diagnostics Tryptic Soy Agar (TSA) contains digests of soybean meal and casein which provide amino acids and other nitrogenous compounds to promote the growth of a variety of microorganisms. Sodium chloride is added to maintain the osmotic equilibrium. Agar is the solidifying agent. Lecithin and Tween® 80 are added to neutralize the  \nantimicrobial effects of disinfectants or cleaning solutions used on environmental surfaces.  \nThe plates are triple bagged and sterilized by irradiation to promote a higher sterility assurance level.  \nFORMULA  \nIngredients per liter ofdeionized water:*  \n\n| TSA contains: |  |\n| --- | --- |\n| Pancreatic Digest of Casein | 15.0gm |\n| Peptic Digest of Soybean Meal | 5.0gm |\n| Sodium Chloride | 5.0gm |\n| Agar | 15.0gm |\n| TSA with Lecithin & Tween® 80 also contains: |  |\n| Lecithin | 0.7gm |\n| Tween® (polysorbate) 80 | 5.0ml |\n\nFinal pH 7.3 +/ -0.2 at 25°C.  \n* Adjusted and/or supplemented as required to meet performance criteria.  \nSTORAGE AND SHELF LIFE  \nStorage: Store at 15-30°C., and store all media away from direct light. Media should not be used if there are any signs of contamination, deterioration, discoloration, or if the expiration date has passed. Product is light and temperature sensitive. Protect from freezing.  \nThe expiration dating on the product label applies to the product in its intact packaging when stored as directed. The product may be used and tested up to the expiration date on the product label and incubated for the recommended quality control incubation times.  \nRefer to the document \"Storage\" for more information.  \nPRECAUTIONS  \nThis product may contain components of animal origin. Certified knowledge of the origin and/or sanitary state of the animals does not guarantee the absence of transmissible pathogenic agents. Therefore, it is recommended tha","cbCaibTQMCm9AqkQ","https://ap.wps.com/l/cbCaibTQMCm9AqkQ","pdf",88835,"English","# Intended Use\n# Summary\n# Formula\n## Ingredients and Composition\n## Final pH\n# Storage and Shelf Life\n# Precautions\n# Procedure","[{\"question\":\"What is Tryptic Soy Agar (TSA), USP, Irradiated used for?\",\"answer\":\"It is recommended as a general growth medium to detect and enumerate microorganisms in the microbiological examination of non-sterile products. It can also support establishing microbiological trends, alerts, and action levels in biologically controlled environments.\"},{\"question\":\"What are the key ingredients and their roles in TSA?\",\"answer\":\"TSA contains digests of soybean meal and casein to provide amino acids and nitrogenous compounds for microbial growth. Sodium chloride maintains osmotic equilibrium, agar solidifies the medium, and lecithin plus Tween 80 help neutralize antimicrobial effects of disinfectants or cleaning solutions.\"},{\"question\":\"How should the irradiated TSA be stored and handled for use?\",\"answer\":\"Store at 15–30°C away from direct light, protect from freezing, and do not use if contamination, deterioration, discoloration, or expired dating is present. Before use, warm to room temperature and ensure the surface is dry; if condensation is observed, remove plates in a sterile environment and allow them to dry for 10–15 minutes.\"}]","W520_Tryptic Soy Agar (TSA) Media, USP, Irradiated | PDF",1789745110,15]