[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"detail-sidebar-cat-0-en-105":3,"doc-seo-618922-105":59,"doc-detail-618922-en":124},{"code":4,"msg":5,"data":6},0,"success",[7,13,18,23,28,33,38,43,48,51,55],{"id":8,"doc_module":4,"doc_module_name":9,"category_name":10,"show_sort_weight":11,"slug":12},1,"Document","Story & Novel",90,"story-novel",{"id":14,"doc_module":4,"doc_module_name":9,"category_name":15,"show_sort_weight":16,"slug":17},2,"Literature",80,"literature",{"id":19,"doc_module":4,"doc_module_name":9,"category_name":20,"show_sort_weight":21,"slug":22},4,"Exam",70,"exam",{"id":24,"doc_module":4,"doc_module_name":9,"category_name":25,"show_sort_weight":26,"slug":27},5,"Comic",60,"comic",{"id":29,"doc_module":4,"doc_module_name":9,"category_name":30,"show_sort_weight":31,"slug":32},6,"Technology",50,"technology",{"id":34,"doc_module":4,"doc_module_name":9,"category_name":35,"show_sort_weight":36,"slug":37},7,"Healthcare",40,"healthcare",{"id":39,"doc_module":4,"doc_module_name":9,"category_name":40,"show_sort_weight":41,"slug":42},8,"Research & Report",30,"research-report",{"id":44,"doc_module":4,"doc_module_name":9,"category_name":45,"show_sort_weight":46,"slug":47},9,"Religion & Spirituality",20,"religion-spirituality",{"id":46,"doc_module":4,"doc_module_name":9,"category_name":49,"show_sort_weight":46,"slug":50},"World Cup","world-cup",{"id":52,"doc_module":4,"doc_module_name":9,"category_name":53,"show_sort_weight":52,"slug":54},10,"Lifestyle","lifestyle",{"id":56,"doc_module":4,"doc_module_name":9,"category_name":57,"show_sort_weight":24,"slug":58},19,"General","general",{"code":4,"msg":60,"data":61},"ok",{"site_id":62,"language":63,"slug":64,"title":65,"keywords":66,"description":67,"schema_data":68,"social_meta":117,"head_meta":119,"extra_data":121,"updated_unix":123},105,"en","viral-infection-mediated-by-filovirus-glycoproteins-requires-npc1-but-not-npc2","Viral infection mediated by filovirus glycoproteins requires NPC1 but not NPC2","","This academic research investigates the role of Niemann-Pick C1 (NPC1) and NPC2 proteins in the entry process of filoviruses. Utilizing comparative analysis between control fibroblasts and those with NPC1 or NPC2 mutations, the study demonstrates that NPC1 is an essential mediator for viral infectivity by filovirus glycoproteins, including Ebolavirus, Sudan virus, and Marburg virus. Conversely, the absence of NPC2 does not significantly impede viral entry, highlighting a specific dependency on the NPC1 pathway. Quantitative assays and imaging techniques confirm that viral infectivity is drastically reduced in NPC1-deficient cells, while the rescue of NPC1 expression restores susceptibility. Additionally, the study identifies U18666A as a potent pharmacological inhibitor of filovirus infection, which targets the pathway associated with NPC1 function. The findings provide critical insights into the molecular mechanisms of viral membrane fusion and potential targets for therapeutic intervention against highly pathogenic filoviruses.",{"@graph":69,"@context":116},[70,84,99],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":40,"@type":76,"position":81},"https://docshare.wps.com/document/research-report/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/viral-infection-mediated-by-filovirus-glycoproteins-requires-npc1-but-not-npc2/618922/",{"url":83,"name":65,"@type":85,"author":86,"headline":65,"publisher":89,"fileFormat":92,"inLanguage":63,"description":67,"dateModified":93,"datePublished":93,"encodingFormat":92,"isAccessibleForFree":94,"interactionStatistic":95},"DigitalDocument",{"name":87,"@type":88},"Margaret","Person",{"url":74,"name":90,"@type":91},"DocShare","Organization","application/octet-stream","2026-10-20",true,{"@type":96,"interactionType":97,"userInteractionCount":4},"InteractionCounter",{"@type":98},"ViewAction",{"@type":100,"mainEntity":101},"FAQPage",[102,108,112],{"name":103,"@type":104,"acceptedAnswer":105},"What is the relationship between NPC1 and filovirus infection?","Question",{"text":106,"@type":107},"The document demonstrates that NPC1 is strictly required for the entry and infectivity of filoviruses mediated by their glycoproteins, as evidenced by significantly reduced infection rates in NPC1-mutant cells.","Answer",{"name":109,"@type":104,"acceptedAnswer":110},"Does NPC2 play a role in filovirus entry similar to NPC1?",{"text":111,"@type":107},"No, the study indicates that while NPC1 is essential, NPC2 is not required for filovirus entry, as NPC2-mutant cells do not exhibit the same drastic reduction in viral infectivity seen in NPC1-mutant cells.",{"name":113,"@type":104,"acceptedAnswer":114},"How does U18666A affect viral infectivity?",{"text":115,"@type":107},"U18666A acts as an inhibitor that reduces the infectivity of viruses containing the Ebolavirus glycoprotein (rVSV-GP-EboV) in a concentration-dependent manner, while showing little effect on control viruses like rVSV-G.","https://schema.org",{"og:url":83,"og:type":118,"og:title":65,"og:site_name":90,"og:description":67},"article",{"robots":120,"canonical":83},"index,follow",{"doc_id":122,"site_id":62},618922,1792462002,{"code":4,"msg":5,"data":125},{"doc_id":122,"user_id":126,"nickname":87,"user_avatar":127,"doc_module":4,"category_id":39,"category_name":40,"doc_title":65,"doc_description":67,"doc_content":128,"file_id":129,"file_url":130,"file_type":131,"file_size":132,"view_count":4,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":8,"language":133,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":134,"faqs":135,"seo_title":136,"seo_description":67,"update_tm":123,"read_time":81},137451207643,"https://ap-avatar.wpscdn.com/davatar_3d24733baf745e90a7e4bdd5f77d97b2","JE Carette et al. Nature 000, 1-4 (2011) doi:10.1038/nature10348\nViral infection mediated by filovirus glycoproteins requires NPC1 but not NPC2.","cbCaiglOVYIglIc5","https://ap.wps.com/l/cbCaiglOVYIglIc5","ppt",641536,"English","# Role of NPC1 and NPC2 in Filovirus Entry\n## Impact of NPC1/NPC2 Mutations on Viral Infectivity\n## Pharmacological Inhibition with U18666A","[{\"question\":\"What is the relationship between NPC1 and filovirus infection?\",\"answer\":\"The document demonstrates that NPC1 is strictly required for the entry and infectivity of filoviruses mediated by their glycoproteins, as evidenced by significantly reduced infection rates in NPC1-mutant cells.\"},{\"question\":\"Does NPC2 play a role in filovirus entry similar to NPC1?\",\"answer\":\"No, the study indicates that while NPC1 is essential, NPC2 is not required for filovirus entry, as NPC2-mutant cells do not exhibit the same drastic reduction in viral infectivity seen in NPC1-mutant cells.\"},{\"question\":\"How does U18666A affect viral infectivity?\",\"answer\":\"U18666A acts as an inhibitor that reduces the infectivity of viruses containing the Ebolavirus glycoprotein (rVSV-GP-EboV) in a concentration-dependent manner, while showing little effect on control viruses like rVSV-G.\"}]","Viral infection mediated by filovirus glycoproteins requires NPC1 but not NPC2 | PPT"]