[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"doc-seo-201975-105":3,"detail-sidebar-cat-0-en-105":80,"doc-detail-201975-en":130},{"code":4,"msg":5,"data":6},0,"ok",{"site_id":7,"language":8,"slug":9,"title":10,"keywords":11,"description":12,"schema_data":13,"social_meta":73,"head_meta":75,"extra_data":77,"updated_unix":79},105,"en","vetscan-prep-profile-ii-reagent-rotor-intended-use-and-test-principles","VetScan Prep Profile II - Reagent Rotor Intended Use and Test Principles","","VetScan Prep Profile II reagent rotor is used with the VetScan Chemistry Analyzer for in vitro quantitative measurements of ALT, ALP, creatinine, glucose, total protein, and urea nitrogen in heparinized whole blood, heparinized plasma, or serum. The system supports veterinary diagnosis by explaining each analyte’s underlying reaction principles, including enzymatic and reference-method basis, reagent interactions, and how absorbance-rate or endpoint changes at specific wavelengths relate to analyte concentration. The guidance emphasizes considering patient clinical status before final diagnosis.",{"@graph":14,"@context":72},[15,34,55],{"@type":16,"itemListElement":17},"BreadcrumbList",[18,23,27,31],{"item":19,"name":20,"@type":21,"position":22},"https://docshare.wps.com","Home","ListItem",1,{"item":24,"name":25,"@type":21,"position":26},"https://docshare.wps.com/document/","Document",2,{"item":28,"name":29,"@type":21,"position":30},"https://docshare.wps.com/document/healthcare/","Healthcare",3,{"item":32,"name":10,"@type":21,"position":33},"https://docshare.wps.com/document/vetscan-prep-profile-ii-reagent-rotor-intended-use-and-test-principles/201975/",4,{"url":32,"name":10,"@type":35,"image":36,"author":41,"headline":10,"publisher":44,"fileFormat":47,"inLanguage":8,"description":12,"dateModified":48,"datePublished":49,"encodingFormat":47,"isAccessibleForFree":50,"interactionStatistic":51},"DigitalDocument",{"url":37,"@type":38,"width":39,"height":40},"https://docshare.wps.com/thumbnails/vetscan-prep-profile-ii-reagent-rotor-intended-use-and-test-principles/201975.png","ImageObject",300,407,{"name":42,"@type":43},"Himbo","Person",{"url":19,"name":45,"@type":46},"DocShare","Organization","application/pdf","2026-09-19","2026-09-04",true,{"@type":52,"interactionType":53,"userInteractionCount":22},"InteractionCounter",{"@type":54},"ViewAction",{"@type":56,"mainEntity":57},"FAQPage",[58,64,68],{"name":59,"@type":60,"acceptedAnswer":61},"What analytes does VetScan Prep Profile II measure with the VetScan Chemistry Analyzer?","Question",{"text":62,"@type":63},"It provides in vitro quantitative determinations of ALT, ALP, creatinine (CRE), glucose (GLU), total protein (TP), and urea nitrogen (BUN).","Answer",{"name":65,"@type":60,"acceptedAnswer":66},"Which sample types are compatible with the reagent rotor?",{"text":67,"@type":63},"It is designed for heparinized whole blood, heparinized plasma, or serum.",{"name":69,"@type":60,"acceptedAnswer":70},"How does the document relate test results to absorbance measurements?",{"text":71,"@type":63},"For each analyte, it explains how the rate of change or endpoint difference in absorbance at specified wavelengths is proportional to analyte concentration in the sample.","https://schema.org",{"og:url":32,"og:type":74,"og:title":10,"og:site_name":45,"og:description":12},"article",{"robots":76,"canonical":32},"index,follow",{"doc_id":78,"site_id":7},201975,1788540536,{"code":4,"msg":81,"data":82},"success",[83,87,91,95,100,105,109,114,119,122,126],{"id":22,"doc_module":4,"doc_module_name":25,"category_name":84,"show_sort_weight":85,"slug":86},"Story & Novel",90,"story-novel",{"id":26,"doc_module":4,"doc_module_name":25,"category_name":88,"show_sort_weight":89,"slug":90},"Literature",80,"literature",{"id":33,"doc_module":4,"doc_module_name":25,"category_name":92,"show_sort_weight":93,"slug":94},"Exam",70,"exam",{"id":96,"doc_module":4,"doc_module_name":25,"category_name":97,"show_sort_weight":98,"slug":99},5,"Comic",60,"comic",{"id":101,"doc_module":4,"doc_module_name":25,"category_name":102,"show_sort_weight":103,"slug":104},6,"Technology",50,"technology",{"id":106,"doc_module":4,"doc_module_name":25,"category_name":29,"show_sort_weight":107,"slug":108},7,40,"healthcare",{"id":110,"doc_module":4,"doc_module_name":25,"category_name":111,"show_sort_weight":112,"slug":113},8,"Research & Report",30,"research-report",{"id":115,"doc_module":4,"doc_module_name":25,"category_name":116,"show_sort_weight":117,"slug":118},9,"Religion & Spirituality",20,"religion-spirituality",{"id":117,"doc_module":4,"doc_module_name":25,"category_name":120,"show_sort_weight":117,"slug":121},"World Cup","world-cup",{"id":123,"doc_module":4,"doc_module_name":25,"category_name":124,"show_sort_weight":123,"slug":125},10,"Lifestyle","lifestyle",{"id":127,"doc_module":4,"doc_module_name":25,"category_name":128,"show_sort_weight":96,"slug":129},19,"General","general",{"code":4,"msg":81,"data":131},{"doc_id":78,"user_id":132,"nickname":42,"user_avatar":133,"doc_module":4,"category_id":106,"category_name":29,"doc_title":10,"doc_description":12,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":22,"is_deleted":4,"is_public":22,"is_downloadable":22,"audit_status":22,"page_count":139,"language":140,"language_code":8,"site_id":7,"html_lang":8,"table_of_contents":141,"faqs":142,"seo_title":143,"seo_description":12,"update_tm":79,"read_time":144},687197100911,"https://ap-avatar.wpscdn.com/avatar/a000239b6f1da00475?x-image-process=image/resize,m_fixed,w_180,h_180&k=1785132997149421697","VetScan® Prep Profile II   \nFor Veterinary use only  \nCustomer and Technical Service 1-800-822-2947  \nJanuary 2015  \nPN: 500-7124, Rev: D  \n© 2002, Abaxis, Inc., Union City, CA 94587  \n1. Intended Use  \nThe VetScan􀂣 Prep Profile II reagent rotor used with the VetScan Chemistry Analyzer utilizes dry and liquid reagents to provide in vitro quantitative determinations of alanine aminotransferase (ALT), alkaline phosphatase (ALP), creatinine (CRE), glucose (GLU), total protein (TP), and urea nitrogen (BUN) in heparinized whole blood, heparinized plasma, or serum.  \n2. Summary and Explanation of Tests  \nThe VetScan Prep Profile II reagent rotor and the VetScan Chemistry Analyzer comprise an in vitro diagnostic system that aids the veterinarian in diagnosing the following disorders:  \nAlanine Aminotransferase Liver diseases; including viral hepatitis and cirrhosis; heart diseases.  \nAlkaline Phosphatase Liver, bone, parathyroid and intestinal diseases.  \nCreatinine Renal disease.  \nGlucose Diabetes, hyperglycemia, hypoglycemia, diabetes and liver disease.  \nTotal Protein Dehydration, kidney, liver disease, metabolic and nutritional disorders.  \nBlood Urea Nitrogen Liver and kidney diseases.  \nAs with any diagnostic test procedure, all other test procedures including the clinical status of the patient should be considered prior to final diagnosis.  \n3. Principles of Procedure  \nAlanine Aminotransferase (ALT)  \nThe method developed for use on the VetScan Chemistry Analyzer is a modification of the Wróblewski and LaDue procedure recommended by the International Federation of Clinical Chemistry (IFCC) . 1, 2  \nIn this reaction, ALT catalyzes the transfer of an amino group from L-alanine to α-ketoglutarate to form L-glutamate and pyruvate. Lactate dehydrogenase catalyzes the conversion of pyruvate to lactate. Concomitantly, NADH is oxidized to NAD+, as illustrated in the following reaction scheme.  \nALT  \nL-Alanine + α-Ketoglutarate  L-Glutamate + Pyruvate  \nLDH  \nPyruvate + NADH + H+  Lactate + NAD+  \nThe rate of change of the absorbance difference between 340 nm and 405 nm is due to the conversion of NADH to NAD+ and is directly proportional to the amount of ALT present in the sample.  \nAlkaline Phosphatase (ALP)  \nThe VetScan procedure is modified from the AACC and IFCC methods. 3 Alkaline phosphatase hydrolyzesp-NPP in a metal-ion buffer and forms p-nitrophenol and phosphate. The use of p-nitrophenyl phosphate (p-NPP) increases the speed of the reaction.4, 5 The reliability of this technique is greatly increased by the use of a metal-ion buffer to maintain the concentration of magnesium and zinc ions in the reaction.6 The American Association for Clinical Chemistry (AACC) reference method uses p-NPP as a substrate and a metal-ion buffer.7  \nALP  \np-Nitrophenyl Phosphate + H2O ~~ ~~ p-Nitrophenol + Phosphate  \nZn2+, Mg2+  \nThe amount of ALP in the sample is proportional to the rate of increase in absorbance difference between 405 nm and 500 nm.  \nCreatinine (CRE)  \nThe Jaffe method, first introduced in 1886, is still a commonly used method of determining creatinine levels in blood. The current reference method combines the use of Fuller’s earth (floridin) with the Jaffe technique to increase the specificity of the reaction.8, 9  \nEnzymatic methods have been developed that are more specific for creatinine than the various modifications ofthe Jaffe technique. 10, 11, 12 Methods using the enzyme creatinine amidohydrolase eliminate the problem of ammonium ion interference found in techniques using creatinine iminohydrolase.13  \nCreatinine Amidohydrolase  \nCreatinine + H2O ~~ ~~ Creatine  \nCreatine Amidinohydrolase  \nCreatine + H2O~~ ~~ Sarcosine + Urea  \nSarcosine Oxidase  \nSarcosine + H2O + O2 ~~ ~~ Glycine + Formaldehyde + H2O2  \nPeroxidase  \nH2O2 + TBHBA + 4-AAP ~~ ~~ Red Quinoneimine Dye + H2O  \nTwo cuvettes are used to determine the concentration of creatinine in the sample. Endogenous creatine is measured in the blank cuvette, which","cbCairDmecFtJqkE","https://ap.wps.com/l/cbCairDmecFtJqkE","pdf",799792,45,"English","# Intended Use\n# Summary and Explanation of Tests\n# Principles of Procedure\n## Alanine Aminotransferase (ALT)\n## Alkaline Phosphatase (ALP)\n## Creatinine (CRE)\n## Glucose (GLU)\n## Total Protein (TP)","[{\"question\":\"What analytes does VetScan Prep Profile II measure with the VetScan Chemistry Analyzer?\",\"answer\":\"It provides in vitro quantitative determinations of ALT, ALP, creatinine (CRE), glucose (GLU), total protein (TP), and urea nitrogen (BUN).\"},{\"question\":\"Which sample types are compatible with the reagent rotor?\",\"answer\":\"It is designed for heparinized whole blood, heparinized plasma, or serum.\"},{\"question\":\"How does the document relate test results to absorbance measurements?\",\"answer\":\"For each analyte, it explains how the rate of change or endpoint difference in absorbance at specified wavelengths is proportional to analyte concentration in the sample.\"}]","VetScan Prep Profile II - Reagent Rotor Intended Use and Test Principles | PDF",113]