[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"detail-sidebar-cat-0-en-105":3,"doc-seo-327541-105":59,"doc-detail-327541-en":131},{"code":4,"msg":5,"data":6},0,"success",[7,13,18,23,28,33,38,43,48,51,55],{"id":8,"doc_module":4,"doc_module_name":9,"category_name":10,"show_sort_weight":11,"slug":12},1,"Document","Story & Novel",90,"story-novel",{"id":14,"doc_module":4,"doc_module_name":9,"category_name":15,"show_sort_weight":16,"slug":17},2,"Literature",80,"literature",{"id":19,"doc_module":4,"doc_module_name":9,"category_name":20,"show_sort_weight":21,"slug":22},4,"Exam",70,"exam",{"id":24,"doc_module":4,"doc_module_name":9,"category_name":25,"show_sort_weight":26,"slug":27},5,"Comic",60,"comic",{"id":29,"doc_module":4,"doc_module_name":9,"category_name":30,"show_sort_weight":31,"slug":32},6,"Technology",50,"technology",{"id":34,"doc_module":4,"doc_module_name":9,"category_name":35,"show_sort_weight":36,"slug":37},7,"Healthcare",40,"healthcare",{"id":39,"doc_module":4,"doc_module_name":9,"category_name":40,"show_sort_weight":41,"slug":42},8,"Research & Report",30,"research-report",{"id":44,"doc_module":4,"doc_module_name":9,"category_name":45,"show_sort_weight":46,"slug":47},9,"Religion & Spirituality",20,"religion-spirituality",{"id":46,"doc_module":4,"doc_module_name":9,"category_name":49,"show_sort_weight":46,"slug":50},"World Cup","world-cup",{"id":52,"doc_module":4,"doc_module_name":9,"category_name":53,"show_sort_weight":52,"slug":54},10,"Lifestyle","lifestyle",{"id":56,"doc_module":4,"doc_module_name":9,"category_name":57,"show_sort_weight":24,"slug":58},19,"General","general",{"code":4,"msg":60,"data":61},"ok",{"site_id":62,"language":63,"slug":64,"title":65,"keywords":66,"description":67,"schema_data":68,"social_meta":124,"head_meta":126,"extra_data":128,"updated_unix":130},105,"en","utilizing-magnetic-levitation-to-detect-lung-cancer-associated-exosomes","Utilizing Magnetic Levitation to Detect Lung Cancer-Associated Exosomes","","Extracellular vesicles, especially exosomes, are explored as novel cancer biomarkers, since exosomal membrane proteins can serve as accessible targets reflecting parent-cell information. This study proposes a MagLev-based, high-sensitivity biosensor for detecting small density differences of suspended objects. Antibody-functionalized microspheres capture ExoMP markers EpCAM, CD81, and CD151 to detect and distinguish cancerous, general, and NSCLC-derived exosomes. The method provides protein-target quantification and demonstrates discrimination using A549 and MRC5 exosome isolates, supporting early lung cancer diagnosis potential.",{"@graph":69,"@context":123},[70,84,106],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":40,"@type":76,"position":81},"https://docshare.wps.com/document/research-report/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/utilizing-magnetic-levitation-to-detect-lung-cancer-associated-exosomes/327541/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/utilizing-magnetic-levitation-to-detect-lung-cancer-associated-exosomes/327541.png","ImageObject",300,407,{"name":92,"@type":93},"Aurora","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-10-29","2026-09-21",true,{"@type":102,"interactionType":103,"userInteractionCount":105},"InteractionCounter",{"@type":104},"ViewAction",15,{"@type":107,"mainEntity":108},"FAQPage",[109,115,119],{"name":110,"@type":111,"acceptedAnswer":112},"What exosomal targets does the MagLev biosensor use for lung cancer detection?","Question",{"text":113,"@type":114},"The sensor uses antibody-functionalized microspheres to capture exosomal membrane proteins EpCAM, CD81, and CD151 as markers for cancerous exosomes, exosomes in general, and NSCLC-derived exosomes, respectively.","Answer",{"name":116,"@type":111,"acceptedAnswer":117},"How does the proposed platform quantify exosome-related biomarkers?",{"text":118,"@type":114},"It measures MagLev heights of the antibody-functionalized polystyrene microspheres before and after capturing target proteins, and performs quantification using image analyses.",{"name":120,"@type":111,"acceptedAnswer":121},"What evidence shows the sensor can distinguish cancerous from non-cancerous exosomes?",{"text":122,"@type":114},"Exosome isolates derived from the NSCLC cell line A549 and the healthy lung fibroblast line MRC5 were tested, and the platform was shown to detect and differentiate biomarkers from cancerous and non-cancerous cell lines.","https://schema.org",{"og:url":83,"og:type":125,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":127,"canonical":83},"index,follow",{"doc_id":129,"site_id":62},327541,1790208386,{"code":4,"msg":5,"data":132},{"doc_id":129,"user_id":133,"nickname":92,"user_avatar":134,"doc_module":4,"category_id":39,"category_name":40,"doc_title":65,"doc_description":67,"doc_content":135,"file_id":136,"file_url":137,"file_type":138,"file_size":139,"view_count":105,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":34,"language":140,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":141,"faqs":142,"seo_title":143,"seo_description":67,"update_tm":144,"read_time":145,"preview_page_count":4},4810365810221,"https://ap-avatar.wpscdn.com/davatar_155a257f0dc6eb9ab79c44ca47cae57d","This article is licensed under CC-BY 4.0   \n[pubs.acs.org/acssensors](pubs.acs.org/acssensors)  Article   \nUtilizing Magnetic Levitation to Detect Lung Cancer-Associated Exosomes  \nAlper Baran Sözmen and Ahu Arslan-Yildiz *  \n Cite This: ACS Sens. 2024, 9, 2043−2049  \nRead Online  \n\n|  |  |  |  |  |  |\n| --- | --- | --- | --- | --- | --- |\n| ACCESS   | Metrics & More |  |  Article Recommendations |  | *sı Supporting Information |\n\nABSTRACT: Extracellular vesicles, especially exosomes, have attracted attention in the last few decades as novel cancer biomarkers. Exosomal membrane proteins provide easy-to-reach targets and can be utilized as information sources of their parent cells. In this study, a MagLev-based, highly sensitive, and versatile biosensor platform for detecting minor differences in the density of suspended objects is proposed for exosome detection. The developed platform utilizes antibody-functionalized microspheres to capture exosomal membrane proteins (ExoMPs) EpCAM, CD81, and CD151 as markers for cancerous exosomes, exosomes, and non-small cell lung cancer (NSCLC)-derived exosomes, respectively. Initially, the platform was utilized for protein detection and quantification by targeting solubilized ExoMPs, and a dynamic  \nrange of 1−100 nM, with LoD values of 1.324, 0.638, and 0.722 nM for EpCAM, CD81, and CD151, were observed, respectively. Then, the sensor platform was tested using exosome isolates derived from NSCLC cell line A549 and MRC5 healthy lung fibroblast cell line. It was shown that the sensor platform is able to detect and differentiate exosomal biomarkers derived from cancerous and non-cancerous cell lines. Overall, this innovative, simple, and rapid method shows great potential for the early diagnosis of lung cancer through exosomal biomarker detection.  \nKEYWORDS: liquid biopsy, lung cancer, magnetic levitation, exosome, exosomal membrane protein detection  \nCancer has become one of the leading causes of death in  \nthe world, and it is one of the diseases with the highest incidence, especially in developed countries.1 Despite the high mortality rate, 40% of cancer cases are curable when diagnosed at an early stage.2,3 However, early diagnosis is difficult due to the exclusion of cancer screening from standard check-up procedures. This exclusion is based on the highly invasive nature of tissue biopsy, which is the current gold standard in cancer diagnosis. Moreover, limited access to healthcare, inadequate diagnostic tools, and lack of symptoms in the early stages also hinder the early diagnosis of the disease.4−6 Hence, in the context of early diagnosis, tissue biopsy is not considered as an effective method.  \nRecent advancements in technology have led to development of non-invasive methods for cancer detection, like liquid biopsy.7,8 Liquid biopsy is a diagnostic method that involves detecting circulating tumor DNA (ctDNA), circulating tumor cells (CTCs) or extracellular vesicles (EVs) in bodily fluids, such as blood or urine. Exosomes are a class of EVs that are intercellular signaling organelles with multiple functions, playing a role in communication, reflecting the cell’s physiological status, and having potential applications in diagnostics.9 Cancer cell-derived exosomes can be utilized to trace the origin, stage, and location of the disease and may hold the potential to become distinctive cancer biomarkers. Current  \nstudies mostly focus on the genetic material that is carried byexosomes. On the other hand, exosomes carry information not only in the form of genetic material but also in their protein content. Therefore, membrane proteins of exosomes (ExoMPs) are easy-to-reach potential biomarkers.10 Tetraspanins such as CD9, CD36, CD44, CD63, CD81, and CD151 are membrane proteins found in exosomes and are investigated as potential biomarkers for various types of cancer. However, due to the requirement of expertise, developed facilities, or both, current ExoMP detection methods such as ELIS","cbCaiqidrh4kC910","https://ap.wps.com/l/cbCaiqidrh4kC910","pdf",4493590,"English","# Abstract\n# Background: Cancer and the need for early detection\n## Liquid biopsy and exosome biomarkers\n# MagLev biosensor approach\n## Target markers and capture strategy\n# Experimental evaluation and results\n## Protein detection and exosome discrimination","[{\"question\":\"What exosomal targets does the MagLev biosensor use for lung cancer detection?\",\"answer\":\"The sensor uses antibody-functionalized microspheres to capture exosomal membrane proteins EpCAM, CD81, and CD151 as markers for cancerous exosomes, exosomes in general, and NSCLC-derived exosomes, respectively.\"},{\"question\":\"How does the proposed platform quantify exosome-related biomarkers?\",\"answer\":\"It measures MagLev heights of the antibody-functionalized polystyrene microspheres before and after capturing target proteins, and performs quantification using image analyses.\"},{\"question\":\"What evidence shows the sensor can distinguish cancerous from non-cancerous exosomes?\",\"answer\":\"Exosome isolates derived from the NSCLC cell line A549 and the healthy lung fibroblast line MRC5 were tested, and the platform was shown to detect and differentiate biomarkers from cancerous and non-cancerous cell lines.\"}]","Utilizing Magnetic Levitation to Detect Lung Cancer-Associated Exosomes | PDF",1789977472,18]