[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"detail-sidebar-cat-0-en-105":3,"doc-seo-447301-105":59,"doc-detail-447301-en":131},{"code":4,"msg":5,"data":6},0,"success",[7,13,18,23,28,33,38,43,48,51,55],{"id":8,"doc_module":4,"doc_module_name":9,"category_name":10,"show_sort_weight":11,"slug":12},1,"Document","Story & Novel",90,"story-novel",{"id":14,"doc_module":4,"doc_module_name":9,"category_name":15,"show_sort_weight":16,"slug":17},2,"Literature",80,"literature",{"id":19,"doc_module":4,"doc_module_name":9,"category_name":20,"show_sort_weight":21,"slug":22},4,"Exam",70,"exam",{"id":24,"doc_module":4,"doc_module_name":9,"category_name":25,"show_sort_weight":26,"slug":27},5,"Comic",60,"comic",{"id":29,"doc_module":4,"doc_module_name":9,"category_name":30,"show_sort_weight":31,"slug":32},6,"Technology",50,"technology",{"id":34,"doc_module":4,"doc_module_name":9,"category_name":35,"show_sort_weight":36,"slug":37},7,"Healthcare",40,"healthcare",{"id":39,"doc_module":4,"doc_module_name":9,"category_name":40,"show_sort_weight":41,"slug":42},8,"Research & Report",30,"research-report",{"id":44,"doc_module":4,"doc_module_name":9,"category_name":45,"show_sort_weight":46,"slug":47},9,"Religion & Spirituality",20,"religion-spirituality",{"id":46,"doc_module":4,"doc_module_name":9,"category_name":49,"show_sort_weight":46,"slug":50},"World Cup","world-cup",{"id":52,"doc_module":4,"doc_module_name":9,"category_name":53,"show_sort_weight":52,"slug":54},10,"Lifestyle","lifestyle",{"id":56,"doc_module":4,"doc_module_name":9,"category_name":57,"show_sort_weight":24,"slug":58},19,"General","general",{"code":4,"msg":60,"data":61},"ok",{"site_id":62,"language":63,"slug":64,"title":65,"keywords":66,"description":67,"schema_data":68,"social_meta":124,"head_meta":126,"extra_data":128,"updated_unix":130},105,"en","usabo-2016-open-exam-mock-practice-questions","USABO 2016 Open Exam - Mock Practice Questions","","USABO Open Exam (February 2 to 12, 2016) provides a set of challenging, multi-topic science multiple-choice questions spanning chemistry, biology, genetics, physiology, molecular biology, microscopy-based plant anatomy, and cancer biology. Problems include selecting properties of water, identifying hormone effects from the anterior pituitary, reasoning about DNA replication and restriction digestion outcomes, interpreting experimental transport states, conducting biomolecule identification from reagents, estimating primer binding-site counts, and evaluating charges at pH 7.0.",{"@graph":69,"@context":123},[70,84,106],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":20,"@type":76,"position":81},"https://docshare.wps.com/document/exam/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/usabo-2016-open-exam-mock-practice-questions/447301/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/usabo-2016-open-exam-mock-practice-questions/447301.png","ImageObject",300,407,{"name":92,"@type":93},"Marglet","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-10-20","2026-09-29",true,{"@type":102,"interactionType":103,"userInteractionCount":105},"InteractionCounter",{"@type":104},"ViewAction",13,{"@type":107,"mainEntity":108},"FAQPage",[109,115,119],{"name":110,"@type":111,"acceptedAnswer":112},"How many questions are included in the provided USABO Open Exam excerpt?","Question",{"text":113,"@type":114},"The excerpt includes questions numbered 1 through 12, though question 12 is cut off mid-sentence.","Answer",{"name":116,"@type":111,"acceptedAnswer":117},"What topics appear across the questions in this excerpt?",{"text":118,"@type":114},"The questions cover physics/chemistry concepts (e.g., properties of water), hormone secretion, DNA replication and restriction digestion, biomolecule identification with chemical reagents, primer binding-site estimation, and pH/charge behavior of amino acids.",{"name":120,"@type":111,"acceptedAnswer":121},"What kind of answer format is used for the questions shown?",{"text":122,"@type":114},"Most questions are multiple-choice with single-answer or SELECT ALL THAT APPLY instructions, and some include ordering of DNA elements or reasoning-based selection from labeled options.","https://schema.org",{"og:url":83,"og:type":125,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":127,"canonical":83},"index,follow",{"doc_id":129,"site_id":62},447301,1790815857,{"code":4,"msg":5,"data":132},{"doc_id":129,"user_id":133,"nickname":92,"user_avatar":134,"doc_module":4,"category_id":19,"category_name":20,"doc_title":65,"doc_description":67,"doc_content":135,"file_id":136,"file_url":137,"file_type":138,"file_size":139,"view_count":105,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":46,"language":140,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":141,"faqs":142,"seo_title":143,"seo_description":67,"update_tm":144,"read_time":31},2336477982702,"https://ap-avatar.wpscdn.com/davatar_9964176cb1d06d4a9deccf72a44ae3dc","USABO Open Exam  \nFebruary 2 to 12, 2016  \n1. Thales is prided as the first Greek philosopher and postulated that water is the unifying principle of the universe. Which of the following is a property of water?  \nA. Water is least dense at 0˚C.  \nB. Linear hydrogen bonds involving water are stronger than covalent bonds.  \nC. Water has a lower heat capacity than methanol  \nD. Hydroxide is the conjugate base of water.  \nE. Water boils at a higher temperature than blood plasma  \n2. Which of the following statements is FALSE regarding the secretion of hormones by the anterior pituitary? SELECT ALL THAT APPLY  \n\n| Hormone | Target | Effect |\n| --- | --- | --- |\n| A. Beta-endorphin | Adrenal gland | Secretion of glucocorticoid |\n| B. Corticotropin | Adrenal gland | Secretion of androgens |\n| C. Prolactin | Mammary glands | Promotes lactation |\n| D. Growth Hormone | Liver | Promotes growth |\n| E. Leptin | Corticotrophic cells | ACTH secretion |\n\n3.  \nYou inserted the coding sequence for your favorite protein into the HindIII site. You find that E. coli carrying this plasmid express the protein in the presence of IPTG, a lactose analog. What DNA elements would you expect to find in proximity to the HindIII site? Select the choice which gives the appropriate elements in the correct order.  \n1. Binding sites for the LacI repressor  \n2. Enhancer sequences  \n3. Poly-A sequence  \n4. Ribosomal binding site  \n5. Stop codon(s)  \n6. Strong constitutive promoter  \nA. 1, 2, 4, [HindIII site], 5, 3  \nB. 2, 1, 4, [HindIII site], 3, 5  \nC. 6, 1, 3, [HindIII site], 4, 5  \nD. 1, 6, 4, [HindIII site] . 3, 5  \nE. 6, 1, 4, [HindIII site], 5, 3  \n4. As DNA replication is occurring, the molecule below is inserted into the nascent DNA strand as it lengthens. Which statement below is TRUE of the progress of replication immediately afterwards?  \nSource: Wikipedia.  \nA. DNA replication would halt because there is an extra 5′-phosphate group for DNA polymerase to add the next nucleotide.  \nB. DNA replication would halt because there is no 3′OH for DNA polymerase to add the next nucleotide.  \nC. DNA replication would halt because there is no 5′OH for DNA polymerase to add the next nucleotide.  \nD. DNA replication would continue, but the new strand would have to be built from 3′ to 5′.  \nE. DNA replication would proceed normally.  \n5. A figure shown below is a transport model for inorganic ions and macromolecules in nuclear pore derived from patch-clamp experiments.  \nIn the absence of macromolecular transport, the electrical conductance (in pS) of the NPC is variable, shifting between open and closed states. In the presence of macromolecule transport, the central channel of the NPC is filled with electrically isolating material that plugs the tunnel. This results in a low passive permeability. In the closed state (closed NPC without transport cargo inside) and in the plugged state (open NPC with electrically isolating transport cargo inside), the patch resistance is high (in GΩ). From the patch-clamp recording graph, which of the following would correspond to the “open” state?  \n2016 USABO Open Exam Page 3  \n6. Consider the following plasmid, pET28a, a popular expression vector that is used for protein expression in bacteria.  \nYou freshly purified plasmid from overnight bacterial culture using extraction kit, also known as miniprep. You confirmed the quality of plasmids by checking DNA and protein ratio (A260/280) and running them on a DNA agarose gel. They looked pure as they should be. You would now like to digest this vector for gene manipulation (molecular cloning) to generate vectors suitable for molecular cloning. If you cut the above plasmid with restriction enzyme called HindIII, the form of DNA will be changed from  \nA. Linear to supercoiled.  \nB. Nicked to linear.  \nC. Nicked to supercoiled.  \nD. Supercoiled to linear.  \nE. Supercoiled to nicked.  \n7. Compound C is a single organic biomolecule with an important role in plant metabolism.  \nSolutions of","cbCaiuNno1vwn0xJ","https://ap.wps.com/l/cbCaiuNno1vwn0xJ","pdf",1095812,"English","# USABO Open Exam Questions\n## Section 1: Science Multiple Choice\n## Section 2: Chemistry and Biology Concepts","[{\"question\":\"How many questions are included in the provided USABO Open Exam excerpt?\",\"answer\":\"The excerpt includes questions numbered 1 through 12, though question 12 is cut off mid-sentence.\"},{\"question\":\"What topics appear across the questions in this excerpt?\",\"answer\":\"The questions cover physics/chemistry concepts (e.g., properties of water), hormone secretion, DNA replication and restriction digestion, biomolecule identification with chemical reagents, primer binding-site estimation, and pH/charge behavior of amino acids.\"},{\"question\":\"What kind of answer format is used for the questions shown?\",\"answer\":\"Most questions are multiple-choice with single-answer or SELECT ALL THAT APPLY instructions, and some include ordering of DNA elements or reasoning-based selection from labeled options.\"}]","USABO 2016 Open Exam - Mock Practice Questions | PDF",1790719703]