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Urinary microbiome shifts have been linked to bladder cancer and may serve as biomarkers, but the mechanisms and diagnostic utility require clarification. Methods analyzed microbiota, metabolites, and cytokines in treatment-naïve patients versus controls, with an additional post-resection timepoint. Results showed proinflammatory cytokine elevation, distinct metabolic alterations, and an ROC-supported biomarker panel. Conclusions support microbiome dysbiosis, inflammation, and altered fatty-acid metabolism in bladder cancer pathogenesis and highlight potential noninvasive diagnostics.",{"@graph":69,"@context":126},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":35,"@type":76,"position":81},"https://docshare.wps.com/document/healthcare/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/urinary-microbiome-dysbiosis-is-associated-with-an-inflammatory-environment-and-perturbed-fatty-acids-metabolism-in-the-pathogenesis-of-bladder-cancer/328308/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/urinary-microbiome-dysbiosis-is-associated-with-an-inflammatory-environment-and-perturbed-fatty-acids-metabolism-in-the-pathogenesis-of-bladder-cancer/328308.png","ImageObject",300,407,{"name":92,"@type":93},"Aria","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-10-23","2026-09-21",true,{"@type":102,"interactionType":103,"userInteractionCount":34},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118,122],{"name":109,"@type":110,"acceptedAnswer":111},"What clinical problem does the study target in bladder cancer management?","Question",{"text":112,"@type":113},"Bladder cancer has a high recurrence rate, and the work focuses on the need for novel minimal-invasive diagnostic tools for earlier diagnosis and recurrence surveillance.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"How were urine samples and data types collected in the study?",{"text":117,"@type":113},"Treatment-naïve bladder cancer patients and non-cancer subjects provided void mid-stream urine, which was analyzed using 16S rDNA sequencing, targeted metabolomic profiling, and flow cytometry after surgery and reallocation into before/after groups.",{"name":119,"@type":110,"acceptedAnswer":120},"Which findings support a link between urinary microbiome dysbiosis, inflammation, and fatty-acid metabolism?",{"text":121,"@type":113},"Before-group cytokines (IL-6, IL-8, IL-10) were elevated, microbiome diversity and microbiota–IL-6 correlations increased, fatty acids and fatty acylcarnitines were enriched, and pathway enrichment implicated fatty-acid beta-oxidation; after resection acylcarnitines decreased while the overall pattern remained similar.",{"name":123,"@type":110,"acceptedAnswer":124},"What diagnostic performance did the proposed urinary biomarker panel achieve?",{"text":125,"@type":113},"ROC analysis indicated that Actinomycetaceae plus arachidonic acid plus IL-6 provided superior performance, with sensitivity of 0.94 and specificity of 1.00.","https://schema.org",{"og:url":83,"og:type":128,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":130,"canonical":83},"index,follow",{"doc_id":132,"site_id":62},328308,1790115240,{"code":4,"msg":5,"data":135},{"doc_id":132,"user_id":136,"nickname":92,"user_avatar":137,"doc_module":4,"category_id":34,"category_name":35,"doc_title":65,"doc_description":67,"doc_content":138,"file_id":139,"file_url":140,"file_type":141,"file_size":142,"view_count":34,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":143,"language":144,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":145,"faqs":146,"seo_title":147,"seo_description":67,"update_tm":148,"read_time":149},2336464648322,"https://ap-avatar.wpscdn.com/avatar/2200025388227c56fec?_k=1778556882303663488","Wu et al. Journal of Translational Medicine (2024) 22:628  \n[https://doi.org/10.1186/s12967-024-05446-7](https://doi.org/10.1186/s12967-024-05446-7)  \nJournal of Translational Medicine  \n RESEARCH Open Access  \nUrinary microbiome dysbiosis is associated   with an inflammatory environment and perturbed fatty acids metabolism  \nin the pathogenesis of bladder cancer  \nCen Wu1†, Xiaoyu Wei1†, Zhiyang Huang1, Zhixiong Zheng1, Wei Zhang1, Jiajun Chen1, Hongchang Hong1 and Weili Li2*  \nAbstract  \nBackground Bladder cancer is a common malignancy with high recurrence rate. Early diagnosis and recurrence surveillance are pivotal to patients’ outcomes, which require novel minimal-invasive diagnostic tools. The urinary microbiome is associated with bladder cancer and can be used as biomarkers, but the underlying mechanism is to be fully illustrated and diagnostic performance to be improved.  \nMethods A total of 23 treatment-naïve bladder cancer patients and 9 non-cancerous subjects were enrolled  \ninto the Before group and Control group. After surgery, 10 patients from the Before group were further assigned into After group. Void mid-stream urine samples were collected and sent for 16S rDNA sequencing, targeted metabolomic profiling, and flow cytometry. Next, correlations were analyzed between microbiota, metabolites, and cytokines. Finally, receiver operating characteristic (ROC) curves of the urinary biomarkers were plotted and compared.  \nResults Comparing to the Control group, levels of IL-6 (p \u003C 0. 01), IL-8 (p \u003C 0. 05), and IL-10 (p \u003C 0. 05) were remarkably elevated in the Before group. The α diversity of urine microbiome was also significantly higher, with the feature microbiota positively correlated to the level of IL-6 (r = 0 . 58, p \u003C 0. 01) . Significant differences in metabolic composition were also observed between the Before and Control groups, with fatty acids and fatty acylcarnitines enriched in the Before group. After tumor resection, cytokine levels and the overall microbiome structure in the After group remained similar to that of the Before group, but fatty acylcarnitines were significantly reduced (p \u003C 0. 05) . Pathway enrichment analysis revealed beta-oxidation of fatty acids was significantly involved (p \u003C 0. 001) . ROC curves showed that the biomarker panel of Actinomycetaceae + arachidonic acid + IL-6 had superior diagnostic performance, with sensitivity of 0.94 and specificity of 1.00.  \nConclusions Microbiome dysbiosis, proinflammatory environment and altered fatty acids metabolism are involved in the pathogenesis of bladder cancer, which may throw light on novel noninvasive diagnostic tool development. Keywords Bladder urothelial carcinoma, Urinary microbiome, Metabolome, Interluekin-6, Inflammation  \n†Cen Wu and Xiaoyu Wei contributed equally to this work.  \n*Correspondence:  \nWeili Li  \n[vera_well4@hotmail.com](vera_well4@hotmail.com)  \nFull list of author information is available at the end of the article  \n© The Author(s) 2024. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit [http://creativecommons.org/licenses/by/4.0/](http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver ()[. The Creative Commons Public Domain Dedicati","cbCaifV6GB4lnJ1F","https://ap.wps.com/l/cbCaifV6GB4lnJ1F","pdf",5364054,17,"English","# Abstract\n## Background\n## Methods\n## Results\n## Conclusions\n# Introduction","[{\"question\":\"What clinical problem does the study target in bladder cancer management?\",\"answer\":\"Bladder cancer has a high recurrence rate, and the work focuses on the need for novel minimal-invasive diagnostic tools for earlier diagnosis and recurrence surveillance.\"},{\"question\":\"How were urine samples and data types collected in the study?\",\"answer\":\"Treatment-naïve bladder cancer patients and non-cancer subjects provided void mid-stream urine, which was analyzed using 16S rDNA sequencing, targeted metabolomic profiling, and flow cytometry after surgery and reallocation into before/after groups.\"},{\"question\":\"Which findings support a link between urinary microbiome dysbiosis, inflammation, and fatty-acid metabolism?\",\"answer\":\"Before-group cytokines (IL-6, IL-8, IL-10) were elevated, microbiome diversity and microbiota–IL-6 correlations increased, fatty acids and fatty acylcarnitines were enriched, and pathway enrichment implicated fatty-acid beta-oxidation; after resection acylcarnitines decreased while the overall pattern remained similar.\"},{\"question\":\"What diagnostic performance did the proposed urinary biomarker panel achieve?\",\"answer\":\"ROC analysis indicated that Actinomycetaceae plus arachidonic acid plus IL-6 provided superior performance, with sensitivity of 0.94 and specificity of 1.00.\"}]","Urinary microbiome dysbiosis is associated with an inflammatory environment and perturbed fatty acids metabolism in the pathogenesis of bladder cancer | PDF",1789979275,43]