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Urine-derived miRNA sequencing defined the miRNA expression landscape in UBC patients and healthy controls, with computational filtering prioritizing mature miRNAs. Quantitative RT-PCR in an age-matched cohort (n=48) validated candidate miRNAs using ROC and AUC metrics. Next-generation sequencing identified 865 annotated and 11 novel miRNAs, and a three-miRNA panel showed strong diagnostic performance, with limitations from single-institute sample size and short follow-up.",{"@graph":69,"@context":126},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":40,"@type":76,"position":81},"https://docshare.wps.com/document/research-report/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/urinary-exosomal-mirna-profiling-reveals-sensitive-non-invasive-detection-of-bladder-cancer/349311/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/urinary-exosomal-mirna-profiling-reveals-sensitive-non-invasive-detection-of-bladder-cancer/349311.png","ImageObject",300,407,{"name":92,"@type":93},"McGucket","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-09-26","2026-09-22",true,{"@type":102,"interactionType":103,"userInteractionCount":19},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118,122],{"name":109,"@type":110,"acceptedAnswer":111},"为什么尿液来源的微RNA（miRNAs）适合作为非侵入性膀胱癌生物标志物？","Question",{"text":112,"@type":113},"研究指出尿液miRNAs具有分子稳定性，并且在肿瘤中呈现具有选择性的表达特征，因此具备作为非侵入性生物标志物的潜力。","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"该研究如何发现并验证候选miRNA生物标志物？",{"text":117,"@type":113},"通过对尿液衍生miRNA进行测序建立表达谱，并进行计算筛选以优先保留成熟miRNA；随后在年龄匹配的独立队列（n=48）中使用qRT-PCR对候选miRNA进行验证，并用ROC曲线与AUC评估诊断性能。",{"name":119,"@type":110,"acceptedAnswer":120},"三种miRNA组成的检测面板的表现如何？",{"text":121,"@type":113},"研究报道三-miRNA面板（miR-6724-5p、miR-10400-5p、miR-7704）具有更优的诊断性能（AUC>70%，敏感性>90%）。",{"name":123,"@type":110,"acceptedAnswer":124},"研究的主要局限性是什么？",{"text":125,"@type":113},"研究局限于单一机构、样本量较小且随访时间较短，因而难以分析已鉴定的miRNA与复发及患者生存之间的相关性。","https://schema.org",{"og:url":83,"og:type":128,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":130,"canonical":83},"index,follow",{"doc_id":132,"site_id":62},349311,1790123948,{"code":4,"msg":5,"data":135},{"doc_id":132,"user_id":136,"nickname":92,"user_avatar":137,"doc_module":4,"category_id":39,"category_name":40,"doc_title":65,"doc_description":67,"doc_content":138,"file_id":139,"file_url":140,"file_type":141,"file_size":142,"view_count":19,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":143,"language":144,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":145,"faqs":146,"seo_title":147,"seo_description":67,"update_tm":148,"read_time":149},1236954412713,"https://us-avatar.wpscdn.com/davatar_29158cc5080c5b710cf443261637dec0","Singh et al. BMC Urology (2026) 26:143 [https://doi.org/10.1186/s12894-026-02130-4](https://doi.org/10.1186/s12894-026-02130-4)  \nBMC Urology  \nRESEARCH Open Access  \nUrinary exosomal miRNA profiling reveals sensitive non-invasive detection of bladder cancer  \nGarima Singh1†, Anil Kumar2†, Lalit Kumar2*, Neelu Mishra1, Shristy Bhattacharjee1, Kunal Yadav1, Yashasvi Singh2, Ujwal Kumar2, Sameer Trivedi2 and Samarendra K. Singh 1*  \nAbstract  \nBackground Urinary microRNAs (miRNAs) hold substantial promise as non-invasive biomarker candidates due to their molecular stability and tumor-selective expression signatures. This study integrates the high-throughput urinary miRNA landscape with clinical data to systematically identify robust, non-invasive biomarkers that facilitate the early diagnosis and better clinical management of urinary bladder cancer (UBC) .  \nMethods Urine-derived miRNA sequencing was performed on UBC patients and healthy controls to delineate the miRNA expression profile. Computational filtering further excluded precursor form of miRNA, prioritizing the mature miRNA. Quantitative RT-PCR targeting an independent, age-matched cohort (n = 48) corroborated with the diagnostic potential of candidate miRNAs, evaluated through receiver operating characteristic (ROC) curves and area under the curve (AUC) analyses.  \nResults and limitations Next-generation sequencing unveiled 865 annotated and 11 novel miRNAs, with UBC samples displaying greater miRNA diversity (708 known miRNAs) compared to relative controls (540) . Among them, seven mature miRNAs were significantly dysregulated in UBC (p \u003C 0. 05), of which quantitative RT-PCR corroborated significant upregulation of miR-6724-5p, miR-1273 h-5p, miR-7704, miR-200-5p, and miR-10400-5p, with fold elevations of 1542. 3, 1085. 8, 1160. 6, 2776 . 5, and 45 . 8, respectively (p \u003C 0. 01) . Stage-stratified profiling underscored dynamic shifts in miRNA expression that correlated with tumor advancement. A three-miRNA panel (miR-6724-5p, miR-10400-5p, and miR-7704) exhibited superior diagnostic performance (AUC > 70%, sensitivity > 90%) . However, this study was constrained to a single institute with a small sample size and a short follow-up period, which precluded the correlation between the identified biomarker miRNAs with cancer recurrence and patient survival.  \nConclusion In this preliminary, single-centre study, mature urinary miRNAs demonstrated reproducible, stagespecific dysregulation in UBC. A combinatorial panel offered potential for a highly sensitive, specific, and non-invasive  \n†Garima Singh and Anil Kumar are equal first author.  \n*Correspondence: Lalit Kumar  \n[lalitkumarims@bhu.ac.in](lalitkumarims@bhu.ac.in); [drlalitkumaragarwal@gmail.com](drlalitkumaragarwal@gmail.com)  \nSamarendra K. Singh  \n[samarendra.singh@bhu.ac.in](samarendra.singh@bhu.ac.in); [biotech3@rediffmail.com](biotech3@rediffmail.com)  \nFull list of author information is available at the end of the article  \n© The Author(s) 2026. Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit [http://crea","cbCaii9WxUeHMoW5","https://ap.wps.com/l/cbCaii9WxUeHMoW5","pdf",3003600,11,"English","# Abstract\n## Background\n## Methods\n## Results and limitations\n## Conclusion\n# Introduction","[{\"question\":\"为什么尿液来源的微RNA（miRNAs）适合作为非侵入性膀胱癌生物标志物？\",\"answer\":\"研究指出尿液miRNAs具有分子稳定性，并且在肿瘤中呈现具有选择性的表达特征，因此具备作为非侵入性生物标志物的潜力。\"},{\"question\":\"该研究如何发现并验证候选miRNA生物标志物？\",\"answer\":\"通过对尿液衍生miRNA进行测序建立表达谱，并进行计算筛选以优先保留成熟miRNA；随后在年龄匹配的独立队列（n=48）中使用qRT-PCR对候选miRNA进行验证，并用ROC曲线与AUC评估诊断性能。\"},{\"question\":\"三种miRNA组成的检测面板的表现如何？\",\"answer\":\"研究报道三-miRNA面板（miR-6724-5p、miR-10400-5p、miR-7704）具有更优的诊断性能（AUC\\u003e70%，敏感性\\u003e90%）。\"},{\"question\":\"研究的主要局限性是什么？\",\"answer\":\"研究局限于单一机构、样本量较小且随访时间较短，因而难以分析已鉴定的miRNA与复发及患者生存之间的相关性。\"}]","Urinary exosomal miRNA profiling reveals sensitive non-invasive detection of bladder cancer | PDF",1790082703,28]