[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"detail-sidebar-cat-0-en-105":3,"doc-seo-329803-105":59,"doc-detail-329803-en":130},{"code":4,"msg":5,"data":6},0,"success",[7,13,18,23,28,33,38,43,48,51,55],{"id":8,"doc_module":4,"doc_module_name":9,"category_name":10,"show_sort_weight":11,"slug":12},1,"Document","Story & Novel",90,"story-novel",{"id":14,"doc_module":4,"doc_module_name":9,"category_name":15,"show_sort_weight":16,"slug":17},2,"Literature",80,"literature",{"id":19,"doc_module":4,"doc_module_name":9,"category_name":20,"show_sort_weight":21,"slug":22},4,"Exam",70,"exam",{"id":24,"doc_module":4,"doc_module_name":9,"category_name":25,"show_sort_weight":26,"slug":27},5,"Comic",60,"comic",{"id":29,"doc_module":4,"doc_module_name":9,"category_name":30,"show_sort_weight":31,"slug":32},6,"Technology",50,"technology",{"id":34,"doc_module":4,"doc_module_name":9,"category_name":35,"show_sort_weight":36,"slug":37},7,"Healthcare",40,"healthcare",{"id":39,"doc_module":4,"doc_module_name":9,"category_name":40,"show_sort_weight":41,"slug":42},8,"Research & Report",30,"research-report",{"id":44,"doc_module":4,"doc_module_name":9,"category_name":45,"show_sort_weight":46,"slug":47},9,"Religion & Spirituality",20,"religion-spirituality",{"id":46,"doc_module":4,"doc_module_name":9,"category_name":49,"show_sort_weight":46,"slug":50},"World Cup","world-cup",{"id":52,"doc_module":4,"doc_module_name":9,"category_name":53,"show_sort_weight":52,"slug":54},10,"Lifestyle","lifestyle",{"id":56,"doc_module":4,"doc_module_name":9,"category_name":57,"show_sort_weight":24,"slug":58},19,"General","general",{"code":4,"msg":60,"data":61},"ok",{"site_id":62,"language":63,"slug":64,"title":65,"keywords":66,"description":67,"schema_data":68,"social_meta":123,"head_meta":125,"extra_data":127,"updated_unix":129},105,"en","unveiling-strs-instability-in-a-colorectal-cancer-ffpe-sample-a-case-report","Unveiling STRs instability in a colorectal cancer FFPE sample - a case report","","Forensic genetics can rely on formalin-fixed paraffin-embedded (FFPE) biopsy material when no other biological samples are available for individual identification or paternity testing. This report analyzes two FFPE samples from the same male: one containing colorectal cancer tissue and one containing histologically normal tissue. Comparing DNA profiles reveals tumor-specific genomic instability affecting multiple STR loci via microsatellite instability and loss of heterozygosity, producing a distorted, fictitious profile.",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":40,"@type":76,"position":81},"https://docshare.wps.com/document/research-report/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/unveiling-strs-instability-in-a-colorectal-cancer-ffpe-sample-a-case-report/329803/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/unveiling-strs-instability-in-a-colorectal-cancer-ffpe-sample-a-case-report/329803.png","ImageObject",300,407,{"name":92,"@type":93},"Aurelia","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-10-23","2026-09-21",true,{"@type":102,"interactionType":103,"userInteractionCount":39},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"Why are FFPE biopsy samples used in forensic genetics?","Question",{"text":112,"@type":113},"FFPE samples taken during life may be the only available material for individual identification or paternity testing.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"What comparison was performed in this case report?",{"text":117,"@type":113},"Two FFPE samples from the same male subject were analyzed: one with colorectal cancer tissue and one with normal histopathological features, followed by STR profile comparison from extracted DNA.",{"name":119,"@type":110,"acceptedAnswer":120},"How did tumor tissue affect the forensic STR results?",{"text":121,"@type":113},"Microsatellite instability and loss of heterozygosity caused instability at multiple STR loci, distorting the tumor-derived genetic profile and yielding a fictitious profile not matching the subject’s true (normal) genotype.","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},329803,1790006535,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":39,"category_name":40,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":39,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":24,"language":139,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":140,"faqs":141,"seo_title":142,"seo_description":67,"update_tm":143,"read_time":144,"preview_page_count":4},1099514068365,"https://ap-avatar.wpscdn.com/avatar/10000253d8d9f28188e?x-image-process=image/resize,m_fixed,w_180,h_180&k=1794309523707217728","International Journal of Legal Medicine (2025) 139:61–65  \n[https://doi.org/10.1007/s00414-024-03341-w](https://doi.org/10.1007/s00414-024-03341-w)  \nCASE REPORT  \nUnveiling STRs instability in a colorectal cancer FFPE sample: a case report  \nGiulia Soldati1 · Chiara Saccardo1 · Dario Raniero1 · Domenico De Leo1 · StefaniaTurrina1  \nReceived: 4 July 2024 / Accepted: 20 September 2024 / Published online: 8 October 2024 © The Author(s) 2024  \nAbstract  \nIn forensic genetics, sometimes formalin-fixed paraffin-embedded (FFPE) biopsy material taken during life is the only biological sample available for individual identification or paternity testing. In most cases, this biological tissue is characterized by the presence of tumor cells characterized by instability and loss of heterozygosity of microsatellites (MSI/ LOH) compared to the DNA present in cells of normal tissue.In this case report, two FFPE samples from the same male subject were available for genetic investigation: one sample with colorectal cancer tissue and the other with normal tissue (no cancerous histopathological features) . The comparison of the genetic profiles obtained from DNA extracted from the two tissues showed in the tumor tissue the presence of three genomic instability phenomena affecting FGA, CSF1P0, D21S2055 loci, located on three distinct autosomal chromosomes, and one duplication phenomenon affecting the DYS438 . Therefore, due to the MSI/LOH phenomena, the genetic profile acquired from the tumor tissue was distorted and thus generated a fictitious genetic profile, not corresponding to the subject’s real one (normal tissue free of tumor cells) .  \nKeywords Instability of microsatellites · Loss of heterozygosity · Cancerous tissues · Formalin fixed paraffin embedded tissues · Forensic genetics profiles · Case report  \nIntroduction  \nWhen no other material is available, archival pathology specimens such as formalin-fixed paraffin-embedded (FFPE) tissues can be used for forensic individual identification or paternity testing. However, as in most cases, these tissues were stored following biopsies performed to check for the presence of cancer cells and identify cancer typology, alterations of short tandem repeats (STRs) used in the forensic field are possible [1] . It is well-documented that cancer tissues may show microsatellite instability (MSI) when thereis the accumulation of somatic alterations in the microsatellite’s length and loss of heterozygosity (LOH) when one or multiple alleles are lost [2–7] . The observation of MSI and LOH in a genetic profile can introduce significant problems in the interpretation of results, leading to false exclusions in  \n􀀍 Giulia Soldati [giulia.soldati_02@univr.it](giulia.soldati_02@univr.it)  \n1 Department of Diagnostics and Public Health, Section of Forensic Medicine, Forensic Genetics Lab, University of Verona, Verona, Italy  \nboth paternity tests and personal identification cases, especially in cases where mutational phenomena occur at more than two loci, as in this specific case report. Different types of tumors and target organs are subject to these genomic alterations; an exceptionally high frequency of these phenomena is seen in colorectal carcinomas, gastrointestinal carcinomas, bladder tumors, and oral cancers. Colorectal cancer (CRC) is the third most common cancer in men and the third most common cause of cancer-related deaths in both men and women, representing a significant public health problem globally [8, 9]. According to current knowledge, CRC develops mainly through a gradual accumulation of genetic and epigenetic alterations in the genome, including widespread microsatellite instabilities.  \nIn this case report, two FFPE samples collected from the same male subject were available for genetic investigation: one sample with CRC tissue and the other with no cancerous histopathological features. From a forensic perspective, the analysis of this casework confirms that great care must be taken when ana","cbCaiduXrOliJL6l","https://ap.wps.com/l/cbCaiduXrOliJL6l","pdf",982657,"English","# Abstract\n# Introduction\n# Materials and methods","[{\"question\":\"Why are FFPE biopsy samples used in forensic genetics?\",\"answer\":\"FFPE samples taken during life may be the only available material for individual identification or paternity testing.\"},{\"question\":\"What comparison was performed in this case report?\",\"answer\":\"Two FFPE samples from the same male subject were analyzed: one with colorectal cancer tissue and one with normal histopathological features, followed by STR profile comparison from extracted DNA.\"},{\"question\":\"How did tumor tissue affect the forensic STR results?\",\"answer\":\"Microsatellite instability and loss of heterozygosity caused instability at multiple STR loci, distorting the tumor-derived genetic profile and yielding a fictitious profile not matching the subject’s true (normal) genotype.\"}]","Unveiling STRs instability in a colorectal cancer FFPE sample - a case report | PDF",1789985676,13]