[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"doc-seo-332658-105":3,"detail-sidebar-cat-0-en-105":81,"doc-detail-332658-en":130},{"code":4,"msg":5,"data":6},0,"ok",{"site_id":7,"language":8,"slug":9,"title":10,"keywords":11,"description":12,"schema_data":13,"social_meta":74,"head_meta":76,"extra_data":78,"updated_unix":80},105,"en","tumour-marker-expression-in-head-and-neck-malignancies-to-identify-potential-targets-for-intraoperative-molecular-nearinfrared-imaging","Tumour Marker Expression in Head and Neck Malignancies to Identify Potential Targets for Intraoperative Molecular Near‑Infrared Imaging","","Oral and laryngeal squamous cell carcinoma (OSCC and LSCC) and papillary thyroid carcinoma (PTC) are common head and neck cancers treated surgically, yet tumour delineation remains difficult, contributing to inadequate resection margins in OSCC/LSCC and missed multifocality in PTC. Near-infrared fluorescence imaging (FLI) with tumour-targeting tracers could improve intraoperative identification and support precise excision. This study evaluates six candidate FLI targets using immunohistochemistry and tumor-tissue expression scoring.",{"@graph":14,"@context":73},[15,34,56],{"@type":16,"itemListElement":17},"BreadcrumbList",[18,23,27,31],{"item":19,"name":20,"@type":21,"position":22},"https://docshare.wps.com","Home","ListItem",1,{"item":24,"name":25,"@type":21,"position":26},"https://docshare.wps.com/document/","Document",2,{"item":28,"name":29,"@type":21,"position":30},"https://docshare.wps.com/document/research-report/","Research & Report",3,{"item":32,"name":10,"@type":21,"position":33},"https://docshare.wps.com/document/tumour-marker-expression-in-head-and-neck-malignancies-to-identify-potential-targets-for-intraoperative-molecular-nearinfrared-imaging/332658/",4,{"url":32,"name":10,"@type":35,"image":36,"author":41,"headline":10,"publisher":44,"fileFormat":47,"inLanguage":8,"description":12,"dateModified":48,"datePublished":49,"encodingFormat":47,"isAccessibleForFree":50,"interactionStatistic":51},"DigitalDocument",{"url":37,"@type":38,"width":39,"height":40},"https://docshare.wps.com/thumbnails/tumour-marker-expression-in-head-and-neck-malignancies-to-identify-potential-targets-for-intraoperative-molecular-nearinfrared-imaging/332658.png","ImageObject",300,407,{"name":42,"@type":43},"Sage","Person",{"url":19,"name":45,"@type":46},"DocShare","Organization","application/pdf","2026-10-28","2026-09-21",true,{"@type":52,"interactionType":53,"userInteractionCount":55},"InteractionCounter",{"@type":54},"ViewAction",7,{"@type":57,"mainEntity":58},"FAQPage",[59,65,69],{"name":60,"@type":61,"acceptedAnswer":62},"What clinical problem does the study address in head and neck cancer surgery?","Question",{"text":63,"@type":64},"Challenges in tumour delineation can lead to inadequate resection margins in OSCC and LSCC and missed multifocality in PTC. The study targets improving intraoperative identification to enable more precise excision.","Answer",{"name":66,"@type":61,"acceptedAnswer":67},"Which tumour-targeting markers were evaluated for fluorescence imaging?",{"text":68,"@type":64},"The study assessed CEA, c-Met, EpCAM, EGFR, integrin αvβ6, and VEGF-α using immunohistochemical staining in OSCC, LSCC, and PTC specimens.",{"name":70,"@type":61,"acceptedAnswer":71},"What markers showed the strongest results for OSCC and LSCC?",{"text":72,"@type":64},"Integrin αvβ6 was significantly overexpressed in OSCC and LSCC, and EGFR showed moderate-high expression in OSCC and universally high expression in LSCC.","https://schema.org",{"og:url":32,"og:type":75,"og:title":10,"og:site_name":45,"og:description":12},"article",{"robots":77,"canonical":32},"index,follow",{"doc_id":79,"site_id":7},332658,1790169824,{"code":4,"msg":82,"data":83},"success",[84,88,92,96,101,106,110,114,119,122,126],{"id":22,"doc_module":4,"doc_module_name":25,"category_name":85,"show_sort_weight":86,"slug":87},"Story & Novel",90,"story-novel",{"id":26,"doc_module":4,"doc_module_name":25,"category_name":89,"show_sort_weight":90,"slug":91},"Literature",80,"literature",{"id":33,"doc_module":4,"doc_module_name":25,"category_name":93,"show_sort_weight":94,"slug":95},"Exam",70,"exam",{"id":97,"doc_module":4,"doc_module_name":25,"category_name":98,"show_sort_weight":99,"slug":100},5,"Comic",60,"comic",{"id":102,"doc_module":4,"doc_module_name":25,"category_name":103,"show_sort_weight":104,"slug":105},6,"Technology",50,"technology",{"id":55,"doc_module":4,"doc_module_name":25,"category_name":107,"show_sort_weight":108,"slug":109},"Healthcare",40,"healthcare",{"id":111,"doc_module":4,"doc_module_name":25,"category_name":29,"show_sort_weight":112,"slug":113},8,30,"research-report",{"id":115,"doc_module":4,"doc_module_name":25,"category_name":116,"show_sort_weight":117,"slug":118},9,"Religion & Spirituality",20,"religion-spirituality",{"id":117,"doc_module":4,"doc_module_name":25,"category_name":120,"show_sort_weight":117,"slug":121},"World Cup","world-cup",{"id":123,"doc_module":4,"doc_module_name":25,"category_name":124,"show_sort_weight":123,"slug":125},10,"Lifestyle","lifestyle",{"id":127,"doc_module":4,"doc_module_name":25,"category_name":128,"show_sort_weight":97,"slug":129},19,"General","general",{"code":4,"msg":82,"data":131},{"doc_id":79,"user_id":132,"nickname":42,"user_avatar":133,"doc_module":4,"category_id":111,"category_name":29,"doc_title":10,"doc_description":12,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":55,"is_deleted":4,"is_public":22,"is_downloadable":22,"audit_status":22,"page_count":123,"language":139,"language_code":8,"site_id":7,"html_lang":8,"table_of_contents":140,"faqs":141,"seo_title":142,"seo_description":12,"update_tm":143,"read_time":144,"preview_page_count":4},687197207057,"https://ap-avatar.wpscdn.com/davatar_29158cc5080c5b710cf443261637dec0","Molecular Diagnosis & Therapy (2024) 28:811–820 [https://doi.org/10.1007/s40291-024-00742-w](https://doi.org/10.1007/s40291-024-00742-w)  \nTumour Marker Expression in Head and Neck Malignancies to Identify Potential Targets for Intraoperative Molecular Near‑Infrared Imaging  \nLorraine J. Lauwerends1 · Bo E. Zweedijk1,2 · Hidde A. Galema1,2 · Lisanne K. A. Neijenhuis3,4,5 ·  \nNeeltje G. Dekker‑Ensink3 · Robert J. Baatenburg de Jong1 · Cornelis Verhoef2 · Shadhvi S. Bhairosingh3 · Peter J. K. Kuppen3 · Alexander L. Vahrmeijer3 · Tessa M. van Ginhoven2 · Senada Koljenović6 · Sjors A. Koppes7 · Denise E. Hilling2,3 · Stijn Keereweer1  \nAccepted: 15 August 2024 / Published online: 9 September 2024 © The Author(s) 2024  \nAbstract  \nBackground Oral and laryngeal squamous cell carcinoma (OSCC and LSCC) and papillary thyroid carcinoma (PTC) are common head and neck cancers (HNCs) typically treated surgically. Challenges in tumour delineation often lead to inadequate resection margins in OSCC and LSCC, and missed multifocality in PTC. Fluorescence imaging (FLI) using near-infrared tumour-targeting tracers may improve intraoperative identification of malignancy, facilitating precise excision. This study evaluates six potential FLI targets in OSCC, LSCC and PTC.  \nMaterials and methods Immunohistochemical staining was performed on OSCC (n = 20), LSCC (n = 10) and PTC (n = 10), assessing CEA, c-Met, EpCAM, EGFR, integrin αvβ6 and VEGF-α . Expression was scored (0–12) using the total immunostaining score (TIS) system, and categorized into absent (TIS 0), low (TIS 1–5), moderate (TIS 6–8) or high (TIS 9–12) . Results Integrin αvβ6 showed significant overexpression in OSCC (TIS: 12; p \u003C 0.001) and LSCC (TIS: 8; p = 0.002), with 80% of OSCC and 90% of LSCC exhibiting moderate-high expression. Similarly, EGFR expression was moderate-high inmost OSCC (87.5%; TIS: 8) and universally high in LSCC (100%; TIS: 12) . In PTC, EGFR and VEGF-α expressions were low-moderate, but significantly higher than in healthy tissue (TIS: 6; p \u003C 0.006) .  \nConclusion This study highlights integrin αvβ6 and EGFR as viable FLI targets in OSCC and LSCC, especially integrin αvβ6 for tumour margin delineation. In PTC, despite lower expressions, the significant overexpression of VEGF-α, c-MET, and EGFR suggests their potential as FLI targets. Our findings support the development of tumour-targeted FLI tracers to improve surgical precision in HNC.  \n* Stijn Keereweer[s.keereweer@erasmusmc.nl](s.keereweer@erasmusmc.nl)  \n1 Department of Otorhinolaryngology, Head and Neck Surgery, Erasmus MC Cancer Institute, Doctor Molewaterplein 40, 3015 GD Rotterdam, The Netherlands  \n2 Department of Surgical Oncology and Gastrointestinal Surgery, Erasmus MC Cancer Institute, Doctor Molewaterplein 40, 3015 GD Rotterdam, The Netherlands  \n1 Introduction  \nOral and laryngeal squamous cell carcinoma (OSSC and LSSC, respectively) and papillary thyroid carcinoma (PTC) are prevalent head and neck cancers (HNCs) . Surgical resection is their cornerstone treatment, yet the management of HNC remains challenging due to their intricate anatomy,  \n3 Department of Surgery, Leiden University Medical Centre,  \nAlbinusdreef 2, 2333 ZA Leiden, The Netherlands  \n4 Centre for Human Drug Research, Zernikedreef 8, 2333 CL Leiden, The Netherlands  \n5 Division of Gastroenterology and Hepatology, Department of Medicine, Stanford University School of Medicine, Stanford, CA 94305, USA  \n6 Department of Pathology, Antwerp University Hospital, 2650 Antwerp, Belgium  \n7 Department of Pathology, Erasmus Medical Centre, Doctor Molewaterplein 40, 3015 GD Rotterdam, The Netherlands  \nproximity to vital structures, and the potential functional impairments following surgery. Precise excision of tumours is crucial in these cases to optimize patient outcomes. Inadequate resection margins [i.e., close (1–5 mm) and positive (≤ 1 mm) resection margins together] are a major negative prognostic factor for overall survival across","cbCaii2xKqQNvwh4","https://ap.wps.com/l/cbCaii2xKqQNvwh4","pdf",1581045,"English","# Abstract\n## Background\n## Materials and methods\n## Results\n## Conclusion\n# Introduction","[{\"question\":\"What clinical problem does the study address in head and neck cancer surgery?\",\"answer\":\"Challenges in tumour delineation can lead to inadequate resection margins in OSCC and LSCC and missed multifocality in PTC. The study targets improving intraoperative identification to enable more precise excision.\"},{\"question\":\"Which tumour-targeting markers were evaluated for fluorescence imaging?\",\"answer\":\"The study assessed CEA, c-Met, EpCAM, EGFR, integrin αvβ6, and VEGF-α using immunohistochemical staining in OSCC, LSCC, and PTC specimens.\"},{\"question\":\"What markers showed the strongest results for OSCC and LSCC?\",\"answer\":\"Integrin αvβ6 was significantly overexpressed in OSCC and LSCC, and EGFR showed moderate-high expression in OSCC and universally high expression in LSCC.\"}]","Tumour Marker Expression in Head and Neck Malignancies to Identify Potential Targets for Intraoperative Molecular Near‑Infrared Imaging | PDF",1789999010,25]