[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"detail-sidebar-cat-0-en-105":3,"doc-seo-297602-105":59,"doc-detail-297602-en":130},{"code":4,"msg":5,"data":6},0,"success",[7,13,18,23,28,33,38,43,48,51,55],{"id":8,"doc_module":4,"doc_module_name":9,"category_name":10,"show_sort_weight":11,"slug":12},1,"Document","Story & Novel",90,"story-novel",{"id":14,"doc_module":4,"doc_module_name":9,"category_name":15,"show_sort_weight":16,"slug":17},2,"Literature",80,"literature",{"id":19,"doc_module":4,"doc_module_name":9,"category_name":20,"show_sort_weight":21,"slug":22},4,"Exam",70,"exam",{"id":24,"doc_module":4,"doc_module_name":9,"category_name":25,"show_sort_weight":26,"slug":27},5,"Comic",60,"comic",{"id":29,"doc_module":4,"doc_module_name":9,"category_name":30,"show_sort_weight":31,"slug":32},6,"Technology",50,"technology",{"id":34,"doc_module":4,"doc_module_name":9,"category_name":35,"show_sort_weight":36,"slug":37},7,"Healthcare",40,"healthcare",{"id":39,"doc_module":4,"doc_module_name":9,"category_name":40,"show_sort_weight":41,"slug":42},8,"Research & Report",30,"research-report",{"id":44,"doc_module":4,"doc_module_name":9,"category_name":45,"show_sort_weight":46,"slug":47},9,"Religion & Spirituality",20,"religion-spirituality",{"id":46,"doc_module":4,"doc_module_name":9,"category_name":49,"show_sort_weight":46,"slug":50},"World Cup","world-cup",{"id":52,"doc_module":4,"doc_module_name":9,"category_name":53,"show_sort_weight":52,"slug":54},10,"Lifestyle","lifestyle",{"id":56,"doc_module":4,"doc_module_name":9,"category_name":57,"show_sort_weight":24,"slug":58},19,"General","general",{"code":4,"msg":60,"data":61},"ok",{"site_id":62,"language":63,"slug":64,"title":65,"keywords":66,"description":67,"schema_data":68,"social_meta":123,"head_meta":125,"extra_data":127,"updated_unix":129},105,"en","tryptic-soy-agar-tsa-pb81-tsa-with-7-sheep-blood","Tryptic Soy Agar (TSA) - PB81 - TSA with 7% Sheep Blood","","Tryptic Soy Agar (TSA) is a general-purpose plating medium used to isolate, cultivate, and maintain a wide range of fastidious and nonfastidious microorganisms. It supports growth of bacteria, yeasts, and molds and includes modifications to improve nutritional or selective performance. PB81 specifies TSA supplemented with 7% defibrinated sheep blood, supporting cultivation, hemolysis-related differential identification, and CAMP testing to evaluate synergistic hemolysis reactions. The document also provides formulation details and recommended inoculation and incubation procedures.",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":35,"@type":76,"position":81},"https://docshare.wps.com/document/healthcare/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/tryptic-soy-agar-tsa-pb81-tsa-with-7-sheep-blood/297602/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/tryptic-soy-agar-tsa-pb81-tsa-with-7-sheep-blood/297602.png","ImageObject",300,407,{"name":92,"@type":93},"supergirl","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-10-23","2026-09-18",true,{"@type":102,"interactionType":103,"userInteractionCount":39},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"What is Tryptic Soy Agar (TSA) used for?","Question",{"text":112,"@type":113},"TSA is used for isolation, cultivation, and maintenance of a variety of fastidious and nonfastidious microorganisms, including bacteria, yeasts, and molds.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"What does PB81 specify in the TSA formulation?",{"text":117,"@type":113},"PB81 refers to TSA supplemented with 7% defibrinated sheep blood.",{"name":119,"@type":110,"acceptedAnswer":120},"How is the CAMP test performed using TSA?",{"text":121,"@type":113},"The procedure uses a known pure culture of Staphylococcus aureus streaked across the plate center, then test organisms streaked perpendicular and close (about 3 mm) without touching, followed by incubation and reading after 18 to 24 hours.","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},297602,1790438856,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":34,"category_name":35,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":39,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":19,"language":139,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":140,"faqs":141,"seo_title":142,"seo_description":67,"update_tm":143,"read_time":52},962088121634,"https://ap-avatar.wpscdn.com/davatar_9964176cb1d06d4a9deccf72a44ae3dc","TRYPTIC SOY AGAR  \n-For in vitro use only  \n Plated Media  Tubed Media  \nPT80 –Tryptic Soy Agar (TSA) TT80 – TSA Slant  \nPT81 – TSA (SXT) TT80-18 – TSA Pour Plate [18-mL]  \nPT89 – TSA w Yeast Extract TB75 – TSA Blood Slant  \nPB75 – TSA w 5% Sheep Blood  \nPB81 – TSA w 7% Sheep Blood PB69 – TSA w 5% Horse Blood  \nPB80 – TSA w 7% Horse Blood  \nTryptic Soy Agar (TSA) is a general purpose plating medium used for the isolation, cultivation, and maintenance of a variety of fastidious and nonfastidious microorganisms.  \nLeavitt et al. demonstrated the versatility of TSA by cultivating both aerobic and anaerobic microbes using TSA. TSA is recognized and recommended by numerous agencies around the world. Our standard formulation is prepared according to the United States Pharmacopeia (USP) and recommended for various different applications put forth by the Association of Official Analytical Chemists (AOAC), the International Dairy Federation (IDF), the United States Department of Agriculture (USDA), and the American Public Health Association (APHA) .  \nTryptic Soy Agar is a highly nutritious base that meets the growth requirements of many types of microorganisms including bacteria, yeasts, and molds. Many modifications have been to the TSA formulation to increase both its nutritious and selective properties. TSA with 5% defibrinated sheep blood is used extensively for the cultivation and recovery of fastidious microbial species and for the determination of hemolytic reactions that are important differential characteristic especially among the streptococci. This medium is also suitable for performing the overnight CAMP test; Group B streptococci produce an extracellular substance (CAMP factor) that can act synergistically with the beta-toxin produced by some Staphylococcus aureus strains. When placed in close proximity to one another the two organisms produce a zone of increased hemolysis.  \nThe CAMP test can also be used to help identify pathogenic species of Listeria.  \nTSA with horse blood is used to isolate more fastidious organisms. Horse blood contains both X and V factor, which are essential growth factors for some organisms such as Haemophilus species. Sheep and human blood are not suitable since they contain specific enzymes that inactivate V Factor.  \nAlthough, some laboratories prefer a plated medium with a higher blood content (7-10%) or with horse blood, these mediums should not be used for determination of hemolytic reactions or for the CAMP test. The increased blood content can make hemolytic reactions less distinct and more difficult to read while defibrinated horse blood, in some instances, has shown to give hemolytic reactions different from sheep blood.  \nTSA supplemented with yeast extract is described in the FDA Bacteriological Analytical Manual for the isolation and purification of Listeria monocytogenes as well as other heterotrophic organisms. Gunn, Ohashi, Gaydos, and Holt developed the selective SXT formulation by adding the selective agents sulfamethoxazole and trimethoprim. They found that this medium gave superior isolation of group A and B streptococci from throat specimens by inhibiting the growth of the normal throat flora.  \nPlain TSA can also be used in the differentiation of Haemophilus species when used in conjunction with X, V, and XV factor disks. Differentiation is based on the growth pattern around the various disks.  \nFormulation per Litre of Medium  \nPT80 & TT80 Tryptic Soy Agar  \nPancreatic Digest of Casein ...........................15.0 g Papaic Digest of Soybean Meal.......................5.0 g Sodium Chloride ..............................................5.0 g Agar ................................................................15.0 g  \npH 7.3 ± 0.2  \nAdditional Ingredients per Liter:  \nPB75 & TB75 TSA with 5% Sheep Blood Defibrinated Sheep Blood.......................... 50.0 mL  \nPB69 TSB with 5% Horse Blood  \nDefibrinated Horse Blood   50.0 mL  \nPB80 TSA with 7% Horse Blood  \nDefibrinated","cbCair5fo5MfVUc3","https://ap.wps.com/l/cbCair5fo5MfVUc3","pdf",38045,"English","# Formulation and Usage\n## Medium Variants (Plated and Tubed)\n## Core Purpose of TSA\n## PB81: TSA with 7% Sheep Blood\n# Composition Per Liter\n## Base Components and pH\n## Additional Ingredients\n# Recommended Procedure\n## Plated and Tubed Inoculation\n## Incubation and Reading Timepoints\n# CAMP Test\n## Procedure Steps\n## Interpretation of Results","[{\"question\":\"What is Tryptic Soy Agar (TSA) used for?\",\"answer\":\"TSA is used for isolation, cultivation, and maintenance of a variety of fastidious and nonfastidious microorganisms, including bacteria, yeasts, and molds.\"},{\"question\":\"What does PB81 specify in the TSA formulation?\",\"answer\":\"PB81 refers to TSA supplemented with 7% defibrinated sheep blood.\"},{\"question\":\"How is the CAMP test performed using TSA?\",\"answer\":\"The procedure uses a known pure culture of Staphylococcus aureus streaked across the plate center, then test organisms streaked perpendicular and close (about 3 mm) without touching, followed by incubation and reading after 18 to 24 hours.\"}]","Tryptic Soy Agar (TSA) - PB81 - TSA with 7% Sheep Blood | PDF",1789746118]