[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"detail-sidebar-cat-0-en-105":3,"doc-seo-510638-105":59,"doc-detail-510638-en":130},{"code":4,"msg":5,"data":6},0,"success",[7,13,18,23,28,33,38,43,48,51,55],{"id":8,"doc_module":4,"doc_module_name":9,"category_name":10,"show_sort_weight":11,"slug":12},1,"Document","Story & Novel",90,"story-novel",{"id":14,"doc_module":4,"doc_module_name":9,"category_name":15,"show_sort_weight":16,"slug":17},2,"Literature",80,"literature",{"id":19,"doc_module":4,"doc_module_name":9,"category_name":20,"show_sort_weight":21,"slug":22},4,"Exam",70,"exam",{"id":24,"doc_module":4,"doc_module_name":9,"category_name":25,"show_sort_weight":26,"slug":27},5,"Comic",60,"comic",{"id":29,"doc_module":4,"doc_module_name":9,"category_name":30,"show_sort_weight":31,"slug":32},6,"Technology",50,"technology",{"id":34,"doc_module":4,"doc_module_name":9,"category_name":35,"show_sort_weight":36,"slug":37},7,"Healthcare",40,"healthcare",{"id":39,"doc_module":4,"doc_module_name":9,"category_name":40,"show_sort_weight":41,"slug":42},8,"Research & Report",30,"research-report",{"id":44,"doc_module":4,"doc_module_name":9,"category_name":45,"show_sort_weight":46,"slug":47},9,"Religion & Spirituality",20,"religion-spirituality",{"id":46,"doc_module":4,"doc_module_name":9,"category_name":49,"show_sort_weight":46,"slug":50},"World Cup","world-cup",{"id":52,"doc_module":4,"doc_module_name":9,"category_name":53,"show_sort_weight":52,"slug":54},10,"Lifestyle","lifestyle",{"id":56,"doc_module":4,"doc_module_name":9,"category_name":57,"show_sort_weight":24,"slug":58},19,"General","general",{"code":4,"msg":60,"data":61},"ok",{"site_id":62,"language":63,"slug":64,"title":65,"keywords":66,"description":67,"schema_data":68,"social_meta":123,"head_meta":125,"extra_data":127,"updated_unix":129},105,"en","trueblack-lipofuscin-autofluorescence-quencher-protocol","TrueBlack - Lipofuscin Autofluorescence Quencher Protocol","","TrueBlack® Lipofuscin Autofluorescence Quencher is formulated to reduce autofluorescence from lipofuscin that accumulates in aged human and animal tissues, while also lowering general background fluorescence. The protocol explains when and how to use the 20X concentrate to quench signal before immunostaining, including compatibility guidance, permeabilization and detergent handling rules, rinse steps, and preparation of a fresh 1:20 dilution in 70% ethanol. It notes possible antibody signal decreases with some immunofluorescence applications.",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":35,"@type":76,"position":81},"https://docshare.wps.com/document/healthcare/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/trueblack-lipofuscin-autofluorescence-quencher-protocol/510638/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/trueblack-lipofuscin-autofluorescence-quencher-protocol/510638.png","ImageObject",300,407,{"name":92,"@type":93},"lugxt","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-11-07","2026-10-05",true,{"@type":102,"interactionType":103,"userInteractionCount":34},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"What does TrueBlack® Lipofuscin Autofluorescence Quencher reduce during experiments?","Question",{"text":112,"@type":113},"It reduces autofluorescence from lipofuscin and also decreases general background fluorescence, including autofluorescence from collagen, elastin, and red blood cells.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"How is the quenching solution prepared for tissue sections?",{"text":117,"@type":113},"Dilute the 20X concentrate 1:20 in 70% ethanol, vortex to mix, and prepare fresh prior to use; heat the vial stock to about 70°C before dilution to help avoid precipitate formation.",{"name":119,"@type":110,"acceptedAnswer":120},"What are the key compatibility and handling constraints?",{"text":121,"@type":113},"It is validated with common counterstains such as DAPI but is not compatible with organic-based mountants and is not compatible with detergent; any detergent steps must be completed before applying the quencher.","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},510638,1791685314,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":34,"category_name":35,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":34,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":14,"language":139,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":140,"faqs":141,"seo_title":142,"seo_description":67,"update_tm":143,"read_time":24,"preview_page_count":4},3985747862600,"https://ap-avatar.wpscdn.com/davatar_9964176cb1d06d4a9deccf72a44ae3dc","Revision 2  \n\\#92401 StoreatRT  \nTrueBlack® Lipofuscin  \nAutofluorescence Quencher  \nSupport: +1-978-867-2388 (U.S.) [cellsignal.com/support](cellsignal.com/support)  \nOrders: 877-616-2355 (U.S.) [orders@cellsignal.com](orders@cellsignal.com)  \nFor Research Use Only. Not for Use in Diagnostic Procedures.  \nDescription: TrueBlack® Lipofuscin Autofluorescence Quencher is specifically formulated to reduce autofluorescence from lipofuscin, a material that can accumulate in aged human and animal tissues. This product can also reduce general background fluorescence, as well as autofluorescence from other sources such as collagen, elastin, and red blood cells.  \nSpecificity/Sensitivity: In addition to significantly reducing autofluorescence, TrueBlack® Lipofuscin Autofluorescence Quencher may cause a decrease in signal with some antibodies by immunofluorescence. We do not find this decrease to be significant enough to limit interpretation of data.  \nConfocal immunofluorescent analysis of fixed frozen mouse kidney, untreated (bottom) or treated with TrueBlack® Lipofuscin Autofluorescence Quencher (top), using AQP4 (D1F8E) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate) \\#82941 (green), α-Smooth Muscle Actin (D4K9N) XP® Rabbit mAb (Alexa Fluor® 555 Conjugate) \\#60839 (red), and DAPI \\#4083 (blue) .  \nStorage: Store at room temperature. Protect from light. This product is stable for 12 months when stored at room temperature.  \nSafety Information: This product contains Dimethylformamide (DMF) . DMF is rapidly absorbed through skin and lungs. It may cause harm to the unborn child. Avoid exposure during pregnancy. If exposed or concerned, get medical advice. See Safety Data Sheet (SDS) .  \nWear personal protective equipment. Avoid contact with skin. Do not handle until all safety precautions have been read and understood.  \nAlexa Fluor is a registered trademark of Life Technologies Corporation. ProLong is a registered trademark of Life Technologies Corporation. TrueBlack® is a registered trademark of Biotium, Inc.  \nAll other trademarks are the property of their respective owners. Visit [cellsignal.com/trademarks](cellsignal.com/trademarks) for more information.  \n[cellsignal.com](cellsignal.com)  \n© 2023 Cell Signaling Technology, Inc.  \nXP and Cell Signaling Technology are trademarks of Cell Signaling Technology, Inc.  \nApplications: W—Western IP—Immunoprecipitation IHC—Immunohistochemistry ChIP—Chromatin Immunoprecipitation IF—Immunofluorescence F—Flow cytometry FC-FP—Flow cytometry-Fixed/Permeabilized FC-L—Flow cytometry-Live E-P—ELISA-Peptide Species Cross-Reactivity: H—human M—mouse R—rat Hm—hamster Mk—monkey Mi—mink C—chicken Dm—D. melanogaster X—Xenopus Z—zebrafish B—bovine Dg—dog Pg—pig Sc—S. cerevisiae Ce—C. elegans Hr—Horse  \nAll—all species expected Species enclosed in parentheses are predicted to react based on 100% homology.  \n\\#[92401](92401 cellsignal.com)[ cellsignal.com](92401 cellsignal.com)  \nTrueBlack® Lipofuscin Autofluorescence Quencher Protocol  \n© 2023 Cell Signaling Technology, Inc.  \nTrueBlack® Lipofuscin Autofluorescence Quencher reacts with lipofuscins to reduce autofluorescence. Consider using this reagent when working with tissue containing lipofuscin (brain, kidney, liver, heart, retina) .  \nIMPORTANT:  \n• TrueBlack® Lipofuscin Autofluorescence Quencher has been validated with many commonly used counterstains such as DAPI. We recommend testing for compatibility with your reagents of interest prior to full scale experimentation.  \n• TrueBlack® Lipofuscin Autofluorescence Quencher is not compatible with organic-based mountants.  \nRequired Materials  \nSupplied  \n1. TrueBlack® Lipofuscin Autofluorescence Quencher  \n(\\#92401): Supplied as 20X concentrate in DMF.  \nNot Supplied  \n1. 70% Ethanol  \n2. 1X Wash Buffer (Phosphate Buffered Saline): To prepare 1L, add 100 mL of 10X Wash Buffer, Phosphate Buffered Saline (\\#12528) to 900 mL reverse osmosis deionized (RODI) or equivalent grade water. Do not add detergent to th","cbCaigm3zxQkGvX7","https://ap.wps.com/l/cbCaigm3zxQkGvX7","pdf",42597694,"English","# Protocol Overview\n## Pre-treatment and Compatibility Notes\n## Required Materials\n## Quenching Steps and Rinse Schedule\n## Storage and Safety Information","[{\"question\":\"What does TrueBlack® Lipofuscin Autofluorescence Quencher reduce during experiments?\",\"answer\":\"It reduces autofluorescence from lipofuscin and also decreases general background fluorescence, including autofluorescence from collagen, elastin, and red blood cells.\"},{\"question\":\"How is the quenching solution prepared for tissue sections?\",\"answer\":\"Dilute the 20X concentrate 1:20 in 70% ethanol, vortex to mix, and prepare fresh prior to use; heat the vial stock to about 70°C before dilution to help avoid precipitate formation.\"},{\"question\":\"What are the key compatibility and handling constraints?\",\"answer\":\"It is validated with common counterstains such as DAPI but is not compatible with organic-based mountants and is not compatible with detergent; any detergent steps must be completed before applying the quencher.\"}]","TrueBlack - Lipofuscin Autofluorescence Quencher Protocol | PDF",1791243463]