[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"detail-sidebar-cat-0-en-105":3,"doc-seo-398682-105":59,"doc-detail-398682-en":131},{"code":4,"msg":5,"data":6},0,"success",[7,13,18,23,28,33,38,43,48,51,55],{"id":8,"doc_module":4,"doc_module_name":9,"category_name":10,"show_sort_weight":11,"slug":12},1,"Document","Story & Novel",90,"story-novel",{"id":14,"doc_module":4,"doc_module_name":9,"category_name":15,"show_sort_weight":16,"slug":17},2,"Literature",80,"literature",{"id":19,"doc_module":4,"doc_module_name":9,"category_name":20,"show_sort_weight":21,"slug":22},4,"Exam",70,"exam",{"id":24,"doc_module":4,"doc_module_name":9,"category_name":25,"show_sort_weight":26,"slug":27},5,"Comic",60,"comic",{"id":29,"doc_module":4,"doc_module_name":9,"category_name":30,"show_sort_weight":31,"slug":32},6,"Technology",50,"technology",{"id":34,"doc_module":4,"doc_module_name":9,"category_name":35,"show_sort_weight":36,"slug":37},7,"Healthcare",40,"healthcare",{"id":39,"doc_module":4,"doc_module_name":9,"category_name":40,"show_sort_weight":41,"slug":42},8,"Research & Report",30,"research-report",{"id":44,"doc_module":4,"doc_module_name":9,"category_name":45,"show_sort_weight":46,"slug":47},9,"Religion & Spirituality",20,"religion-spirituality",{"id":46,"doc_module":4,"doc_module_name":9,"category_name":49,"show_sort_weight":46,"slug":50},"World Cup","world-cup",{"id":52,"doc_module":4,"doc_module_name":9,"category_name":53,"show_sort_weight":52,"slug":54},10,"Lifestyle","lifestyle",{"id":56,"doc_module":4,"doc_module_name":9,"category_name":57,"show_sort_weight":24,"slug":58},19,"General","general",{"code":4,"msg":60,"data":61},"ok",{"site_id":62,"language":63,"slug":64,"title":65,"keywords":66,"description":67,"schema_data":68,"social_meta":124,"head_meta":126,"extra_data":128,"updated_unix":130},105,"en","triple-sugar-iron-agar-dehydrated-culture-media-for-microbiology-preparation","Triple Sugar Iron Agar (Dehydrated Culture Media) for Microbiology Preparation","","Product code 413771 describes Triple Sugar Iron Agar (TSI), a dehydrated microbiological culture medium. It provides preparation instructions: dissolve 64.6 g in 1 litre distilled water, heat with frequent agitation, boil for one minute, dispense into tubes, autoclave at 121ºC for 15 minutes, and cool in a slanted position to form 1.5–2.0 cm butts. Storage is 2–8°C. TSI differentiates enteric Gram-negative Enterobacteria via carbohydrate fermentation and H2S production, producing characteristic colour and blackening patterns, with incubation at 35±2°C for 18–72 hours.",{"@graph":69,"@context":123},[70,84,106],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":35,"@type":76,"position":81},"https://docshare.wps.com/document/healthcare/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/triple-sugar-iron-agar-dehydrated-culture-media-for-microbiology-preparation/398682/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/triple-sugar-iron-agar-dehydrated-culture-media-for-microbiology-preparation/398682.png","ImageObject",300,407,{"name":92,"@type":93},"Bintang","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-11-02","2026-09-27",true,{"@type":102,"interactionType":103,"userInteractionCount":105},"InteractionCounter",{"@type":104},"ViewAction",12,{"@type":107,"mainEntity":108},"FAQPage",[109,115,119],{"name":110,"@type":111,"acceptedAnswer":112},"How do you prepare Triple Sugar Iron Agar (TSI) from the dehydrated medium?","Question",{"text":113,"@type":114},"Suspend 64.6 g in 1 litre of distilled water, mix and dissolve by heating with frequent agitation, then boil for one minute. Dispense into tubes and sterilize in an autoclave at 121ºC for 15 minutes, then cool in a slanted position to obtain butts 1.5–2.0 cm deep.","Answer",{"name":116,"@type":111,"acceptedAnswer":117},"What is the purpose of TSI in microbiology testing?",{"text":118,"@type":114},"TSI is a differential medium used to differentiate enteric Gram-negative Enterobacteria based on carbohydrate fermentation and H2S production, supporting identification of pathogenic and saprophytic organisms from routine bacteriological samples.",{"name":120,"@type":111,"acceptedAnswer":121},"How are suspected Salmonella results provisionally confirmed using TSI?",{"text":122,"@type":114},"After separate surface and deep inoculation, Salmonella is provisionally confirmed when the deep culture changes from red to yellow and usually forms gas, with or without hydrogen sulfide production in the agar.","https://schema.org",{"og:url":83,"og:type":125,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":127,"canonical":83},"index,follow",{"doc_id":129,"site_id":62},398682,1790753823,{"code":4,"msg":5,"data":132},{"doc_id":129,"user_id":133,"nickname":92,"user_avatar":134,"doc_module":4,"category_id":34,"category_name":35,"doc_title":65,"doc_description":67,"doc_content":135,"file_id":136,"file_url":137,"file_type":138,"file_size":139,"view_count":105,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":14,"language":140,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":141,"faqs":142,"seo_title":143,"seo_description":67,"update_tm":144,"read_time":24,"preview_page_count":4},962085564381,"https://ap-avatar.wpscdn.com/davatar_6f874abed73319feea01a86fa6f0fab8","PRODUCT CODE: 413771  \nTriple Sugar Iron Agar (Dehydrated Culture Media) for microbiology Preparation  \nSuspend 64.6 grams of the medium in one litre of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Dispense into tubes and sterilize in autoclave at 121ºC for 15 minutes. Allow to cool in a slanted position in order to obtain butts of 1.5-2.0 cm. depth.  \nThe prepared medium should be stored at 2-8°C. The colour is red. The dehydrated medium should be homogeneous, free-flowing and pink in colour. If there are any physical changes, discard the medium.  \nUses  \nTRIPLE SUGAR IRON AGAR (TSI) is a differential medium used to differentiate enteric Gram-negative Enterobacteria on the basis of carbohydrate fermentation and H2S production. It is used as an aid in the identification of pathogenic and saprophytic Enterobacteria isolated from routine bacteriological analysis of material samples such as feces.  \nThis medium is used to initiate the identification of Enterobacteria in some FDA schemas. Peptone mixture and the Beef extract provide nitrogen, vitamins, minerals and amino acids essential for growth. Yeast extract is a source of vitamins, particularly of the B-group. TSI contains three carbohydrates (Dextrose, Sucrose and Lactose) as sources of carbon and energy. When these are fermented the acid, production is indicated by the Phenol red indicator, being the colour changes yellow for acid production and red for alkalinisation. Sodium thiosulfate is reduced to Hydrogen sulfide, which reacts with the iron salt to give the black iron sulfide. Ferric ammonium citrate is a H2S indicator. Sodium chloride supplies essential electrolytes for transport and osmotic balance.  \nBacteriological agar is the solidifying agent. The mode of action is similar to Kligler Iron Agar which contains two sugars. The addition of 1% Sucrose in TSI Agar allows to differentiate between Proteus and Salmonella. The fermentation of sucrose by Proteus turns the colour of the Phenol red indicator in the slant from red to yellow. Dextrose-positive and lactose-negative members of the genus Salmonella all cause a reddening of the slant and acidify the depths of the agar tubes.  \nThe European Pharmacopoeia recommends Triple Sugar Iron (TSI) Agar as one of the mediums used for the confirmation of Salmonella. The Eur. Pharmacopoeia recommends transferring separately a few of the suspect colonies to TSI Agar using surface and deep inoculation. The presence of salmonellae is provisionally confirmed if in the deep inoculation, but not in the surface culture, there is a change of colour from red to yellow and, usually, a formation of gas, with or without production of Hydrogen sulphide in the agar.  \nPrecise confirmation may be carried out by the appropriate biochemical and serological tests. The product passes the test if colonies of the type described do not appear, or if the confirmatory biochemical and serological tests are negative. Inoculate with sample and incubate at 35±2 for 18-72 hours.  \nComposition  \nSee in Data Sheet (TDS) .  \nMicrobiological Test  \nThe following results were obtained in the performance of the medium from type cultures after incubation at a temperature of 35±2ºC and observed after 18-72 hours.  \n\n| Microorganism | Growth | Slant | Depth | H2S | Gas |\n| --- | --- | --- | --- | --- | --- |\n| Escherichia coli ATCC 25922 | Good | Yellow | Yellow | - | + |\n| Proteus vulgaris ATCC 13315 | Good | Yellow | Yellow | + | + |\n| Salmonella typhimurium ATCC 14028 | Good | Red | Yellow | + | + |\n| Shigella flexneri ATCC 12022 | Good | Red | Yellow | - | - |\n\nStorage  \nOnce opened keep powdered medium closed to avoid hydration.  \nJM180913","cbCaic4UhkZHbdjv","https://ap.wps.com/l/cbCaic4UhkZHbdjv","pdf",110040,"English","# Preparation\n## Uses and Principle\n## Composition and Media Indicators\n## Inoculation and Incubation\n# Microbiological Test Results\n# Storage","[{\"question\":\"How do you prepare Triple Sugar Iron Agar (TSI) from the dehydrated medium?\",\"answer\":\"Suspend 64.6 g in 1 litre of distilled water, mix and dissolve by heating with frequent agitation, then boil for one minute. Dispense into tubes and sterilize in an autoclave at 121ºC for 15 minutes, then cool in a slanted position to obtain butts 1.5–2.0 cm deep.\"},{\"question\":\"What is the purpose of TSI in microbiology testing?\",\"answer\":\"TSI is a differential medium used to differentiate enteric Gram-negative Enterobacteria based on carbohydrate fermentation and H2S production, supporting identification of pathogenic and saprophytic organisms from routine bacteriological samples.\"},{\"question\":\"How are suspected Salmonella results provisionally confirmed using TSI?\",\"answer\":\"After separate surface and deep inoculation, Salmonella is provisionally confirmed when the deep culture changes from red to yellow and usually forms gas, with or without hydrogen sulfide production in the agar.\"}]","Triple Sugar Iron Agar (Dehydrated Culture Media) for Microbiology Preparation | PDF",1790488046]