[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"detail-sidebar-cat-0-en-105":3,"doc-seo-398705-105":59,"doc-detail-398705-en":130},{"code":4,"msg":5,"data":6},0,"success",[7,13,18,23,28,33,38,43,48,51,55],{"id":8,"doc_module":4,"doc_module_name":9,"category_name":10,"show_sort_weight":11,"slug":12},1,"Document","Story & Novel",90,"story-novel",{"id":14,"doc_module":4,"doc_module_name":9,"category_name":15,"show_sort_weight":16,"slug":17},2,"Literature",80,"literature",{"id":19,"doc_module":4,"doc_module_name":9,"category_name":20,"show_sort_weight":21,"slug":22},4,"Exam",70,"exam",{"id":24,"doc_module":4,"doc_module_name":9,"category_name":25,"show_sort_weight":26,"slug":27},5,"Comic",60,"comic",{"id":29,"doc_module":4,"doc_module_name":9,"category_name":30,"show_sort_weight":31,"slug":32},6,"Technology",50,"technology",{"id":34,"doc_module":4,"doc_module_name":9,"category_name":35,"show_sort_weight":36,"slug":37},7,"Healthcare",40,"healthcare",{"id":39,"doc_module":4,"doc_module_name":9,"category_name":40,"show_sort_weight":41,"slug":42},8,"Research & Report",30,"research-report",{"id":44,"doc_module":4,"doc_module_name":9,"category_name":45,"show_sort_weight":46,"slug":47},9,"Religion & Spirituality",20,"religion-spirituality",{"id":46,"doc_module":4,"doc_module_name":9,"category_name":49,"show_sort_weight":46,"slug":50},"World Cup","world-cup",{"id":52,"doc_module":4,"doc_module_name":9,"category_name":53,"show_sort_weight":52,"slug":54},10,"Lifestyle","lifestyle",{"id":56,"doc_module":4,"doc_module_name":9,"category_name":57,"show_sort_weight":24,"slug":58},19,"General","general",{"code":4,"msg":60,"data":61},"ok",{"site_id":62,"language":63,"slug":64,"title":65,"keywords":66,"description":67,"schema_data":68,"social_meta":123,"head_meta":125,"extra_data":127,"updated_unix":129},105,"en","triple-sugar-iron-agar-bis-technical-details","Triple Sugar Iron Agar BIS - Technical Details","","Triple Sugar Iron Agar BIS is a selective differential medium used to identify Gram-negative enteric bacilli by monitoring dextrose, lactose and sucrose fermentation along with hydrogen sulphide production, following BIS specification IS: 5887 (Part V)-1976. The formulation includes peptone, yeast extract, beef extract, salts, fermentable carbohydrates, H2S indicators (sodium thiosulphate and iron (III) citrate system) and phenol red for pH detection. Acid/gas responses produce color and blackening patterns in slants and butts.",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":30,"@type":76,"position":81},"https://docshare.wps.com/document/technology/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/triple-sugar-iron-agar-bis-technical-details/398705/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/triple-sugar-iron-agar-bis-technical-details/398705.png","ImageObject",300,407,{"name":92,"@type":93},"Jasmine","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-10-16","2026-09-27",true,{"@type":102,"interactionType":103,"userInteractionCount":29},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"What is the intended purpose of Triple Sugar Iron Agar BIS?","Question",{"text":112,"@type":113},"It is used for identification of Gram-negative enteric bacilli based on dextrose, lactose and sucrose fermentation and hydrogen sulphide production in compliance with BIS IS: 5887 (Part V)-1976.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"How do acid, gas, and H2S results appear in the slant and butt?",{"text":117,"@type":113},"Acid production changes the phenol red indicator from red to yellow; gas causes splitting/cracking; H2S is indicated by a black precipitate that blackens the medium in the butt.",{"name":119,"@type":110,"acceptedAnswer":120},"What are key procedural and handling precautions?",{"text":121,"@type":113},"Ensure proper sample labelling and follow established handling guidelines; stab the butt to avoid invalidating the test, and do not inoculate using an inoculating loop because mechanical splitting can cause false positive gas results.","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},398705,1791437119,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":29,"category_name":30,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":29,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":81,"language":139,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":140,"faqs":141,"seo_title":142,"seo_description":67,"update_tm":143,"read_time":39},2336478487870,"https://ap-avatar.wpscdn.com/davatar_085a072bc5b1113ac321206ff7593b45","Triple Sugar Iron Agar BIS  \nIntended Use  \nTriple Sugar Iron Agar BIS is used for the identification of Gram-negative enteric bacilli on the basis of dextrose, lactose and sucrose fermentation and hydrogen sulphide production in compliance with BIS specification IS: 5887 (Part V)-1976 .  \nSummary  \nSulkin and Willett originally developed Triple Sugar Iron Agar, which was later modified by Hajna by adding sucrose to the double sugar (dextrose and lactose) formulation of Kligler Iron Agar. The addition of sucrose increased the sensitivity of the medium by facilitating the detection of sucrose fermenting bacilli as well as lactose and/or dextrose fermenters. Acid and gas production is an indication of carbohydrate fermentation, which gives a visible colour change from red to yellow due to change in the phenol red indicator. The production of hydrogen sulphide is indicated by the presence of a precipitate that blackens the medium in the butt of the tube.  \nThe medium complies with the recommendations of APHA for examination of food, dairy, water and wastewater and for microbial limit test in confirming the presence of Salmonella and in the identification of Gram-negative bacilli. Triple Sugar Iron Agar is also included in the Bacteriological Analytical Manual for food and cosmetics testing.  \nPrinciple  \nPeptone, yeast extract and beef extract provide nitrogenous compounds, sulphur, trace elements, vitamin B complex, etc. while sodium chloride maintains the osmotic equilibrium. Lactose, sucrose and glucose are the fermentable carbohydrates. Sodium thiosulphate and Iron (III) Citrate make the H2S indicator system. Phenol red is the pH indicator.  \nFormula*  \nIngredients g/L  \nLactose 10.0  \nSucrose 10.0  \nPeptone 20.0  \nSodium Chloride 5.0  \nYeast Extract 3.0  \nMeat Extract 3.0  \nGlucose 1.0  \nSodium Thiosulphate, Pentahydrate 0.3  \nFerrous Sulphate Heptahydrate 0.2  \nPhenol Red 0.024  \nAgar 12.0  \nFinal pH (at 25°C) 7.4 ± 0.2  \n*Adjusted to suit performance parameters.  \nStorage and Stability  \nStore dehydrated medium below 30°C in tightly closed container and the prepared medium at 2°C-8°C. Avoid freezing and overheating. Use before expiry date on the label. Once opened keep powdered medium closed to avoid hydration.  \nType of Specimen  \nFood and dairy sample; Water and waste water sample  \nSpecimen Collection and Handling  \nEnsure that all samples are properly labelled.  \nFollow appropriate techniques for handling samples as per established guidelines.  \nSome samples may require special handling, such as immediate refrigeration or protection from light, follow the standard procedure.  \nThe samples must be stored and tested within the permissible time duration.  \nAfter use, contaminated materials must be sterilized by autoclaving before discarding.  \nDirections  \n1. Suspend 64.62 g of the powder in 1000 mL purified / distilled water and mix thoroughly.  \n2. Boil with frequent agitation to dissolve the powder completely.  \n3. Dispense in desired containers as per requirements.  \n4. Sterilize by autoclaving at 115°C (10 psi) for 15 minutes as per validated cycle.  \n5. Allow the medium to set in sloped form with a butt about 1 inch long.  \nQuality Control  \nDehydrated Appearance: Light yellow to pink coloured, homogenous, free flowing powder.  \nPrepared Appearance: Pinkish red coloured, clear to slightly opalescent gel forms in tubes as slants. Cultural Response: Cultural characteristics observed after an incubation of 18-24 hours at 30°C-35°C.  \nOrganism (ATCC)  \nSalmonella enterica subsp. entericaserovar Typhimurium (14028) Escherichia coli (25922)  \nShigella flexneri serotype 2b (12022) Pseudomonas aeruginosa Strain Boston 41501 (27853)  \nGrowth  \nGood  \nGood  \nGood  \nGood  \nSlant  \nK  \nA  \nK  \nK  \nButt Gas  \nA +  \nA +  \nA   \nK -  \nH2S  \n+  \n-  \n-  \n-  \nKey:  \nA = Acidic (Colour of the media changed to yellow)  \nK = Alkaline (No change in colour of the media or the colour of the media changed to pink)  \n+ = Positive (For H2S pos","cbCait2FgG9gDYhx","https://ap.wps.com/l/cbCait2FgG9gDYhx","pdf",152843,"English","# Intended Use\n# Summary\n# Principle\n# Formula\n# Storage and Stability\n# Type of Specimen\n# Specimen Collection and Handling\n# Directions\n# Quality Control\n## Organism (ATCC)\n## Growth and Reactions\n# Interpretation of Results\n# Performance and Evaluation\n# Precautions/Limitations","[{\"question\":\"What is the intended purpose of Triple Sugar Iron Agar BIS?\",\"answer\":\"It is used for identification of Gram-negative enteric bacilli based on dextrose, lactose and sucrose fermentation and hydrogen sulphide production in compliance with BIS IS: 5887 (Part V)-1976.\"},{\"question\":\"How do acid, gas, and H2S results appear in the slant and butt?\",\"answer\":\"Acid production changes the phenol red indicator from red to yellow; gas causes splitting/cracking; H2S is indicated by a black precipitate that blackens the medium in the butt.\"},{\"question\":\"What are key procedural and handling precautions?\",\"answer\":\"Ensure proper sample labelling and follow established handling guidelines; stab the butt to avoid invalidating the test, and do not inoculate using an inoculating loop because mechanical splitting can cause false positive gas results.\"}]","Triple Sugar Iron Agar BIS - Technical Details | PDF",1790488284]