[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"detail-sidebar-cat-0-en-105":3,"doc-seo-513277-105":59,"doc-detail-513277-en":130},{"code":4,"msg":5,"data":6},0,"success",[7,13,18,23,28,33,38,43,48,51,55],{"id":8,"doc_module":4,"doc_module_name":9,"category_name":10,"show_sort_weight":11,"slug":12},1,"Document","Story & Novel",90,"story-novel",{"id":14,"doc_module":4,"doc_module_name":9,"category_name":15,"show_sort_weight":16,"slug":17},2,"Literature",80,"literature",{"id":19,"doc_module":4,"doc_module_name":9,"category_name":20,"show_sort_weight":21,"slug":22},4,"Exam",70,"exam",{"id":24,"doc_module":4,"doc_module_name":9,"category_name":25,"show_sort_weight":26,"slug":27},5,"Comic",60,"comic",{"id":29,"doc_module":4,"doc_module_name":9,"category_name":30,"show_sort_weight":31,"slug":32},6,"Technology",50,"technology",{"id":34,"doc_module":4,"doc_module_name":9,"category_name":35,"show_sort_weight":36,"slug":37},7,"Healthcare",40,"healthcare",{"id":39,"doc_module":4,"doc_module_name":9,"category_name":40,"show_sort_weight":41,"slug":42},8,"Research & Report",30,"research-report",{"id":44,"doc_module":4,"doc_module_name":9,"category_name":45,"show_sort_weight":46,"slug":47},9,"Religion & Spirituality",20,"religion-spirituality",{"id":46,"doc_module":4,"doc_module_name":9,"category_name":49,"show_sort_weight":46,"slug":50},"World Cup","world-cup",{"id":52,"doc_module":4,"doc_module_name":9,"category_name":53,"show_sort_weight":52,"slug":54},10,"Lifestyle","lifestyle",{"id":56,"doc_module":4,"doc_module_name":9,"category_name":57,"show_sort_weight":24,"slug":58},19,"General","general",{"code":4,"msg":60,"data":61},"ok",{"site_id":62,"language":63,"slug":64,"title":65,"keywords":66,"description":67,"schema_data":68,"social_meta":123,"head_meta":125,"extra_data":127,"updated_unix":129},105,"en","translational-stroke-research-1215-lipooxygenase-inhibition","Translational Stroke Research - 12/15-Lipooxygenase Inhibition","","12/15-Lipooxygenase (12/15-LOX) overexpression has been linked to worse early outcomes after subarachnoid hemorrhage (SAH), but its role in delayed events remained unclear. Using endovascular perforation in C57BL/6 and 12/15-LOX−/− mice, researchers measured 12/15-LOX expression, microvessel constriction, microthrombi, neurodegeneration, infarction, perfusion, and delayed deficits, including a platelet spreading assay in patient samples. 12/15-LOX inhibition improved perfusion and reduced delayed pathology, including platelet activation.",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":35,"@type":76,"position":81},"https://docshare.wps.com/document/healthcare/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/translational-stroke-research-1215-lipooxygenase-inhibition/513277/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/translational-stroke-research-1215-lipooxygenase-inhibition/513277.png","ImageObject",300,407,{"name":92,"@type":93},"BAd apple","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-11-12","2026-10-06",true,{"@type":102,"interactionType":103,"userInteractionCount":52},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"What delayed pathological effects were targeted after subarachnoid hemorrhage (SAH)?","Question",{"text":112,"@type":113},"The study focused on delayed cerebral ischemia–related mechanisms, including microvessel constrictions, microthrombi formation, neuronal degeneration, and infarction.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"How was 12/15-LOX inhibition tested in the mouse model?",{"text":117,"@type":113},"C57BL/6 mice were administered the 12/15-LOX inhibitor ML351 or vehicle after SAH, then evaluated over days for neuroscore, cerebral 12/15-LOX expression, vessel constrictions, platelet activation, microthrombi, perfusion, and delayed deficits.",{"name":119,"@type":110,"acceptedAnswer":120},"Does 12/15-LOX inhibition affect platelet activation in human samples?",{"text":121,"@type":113},"Yes. The effect of 12/15-LOX inhibition on platelet activation was assessed in SAH patient samples using a platelet spreading assay, and inhibition reduced platelet activation in both human and mouse blood samples.","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},513277,1791787844,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":34,"category_name":35,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":52,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":139,"language":140,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":141,"faqs":142,"seo_title":143,"seo_description":67,"update_tm":144,"read_time":145,"preview_page_count":4},19241458193282,"https://us-avatar.wpscdn.com/davatar_a8503ba1806abce46bf441b54a3ca4cd","Translational Stroke Research (2025) 16:1156–1172 [https://doi.org/10.1007/s12975-024-01295-0](https://doi.org/10.1007/s12975-024-01295-0)  \n12/15‑Lipooxygenase Inhibition Reduces Microvessel Constriction and Microthrombi After Subarachnoid Hemorrhage in Mice  \nAri Dienel1 · Sung Ha Hong1 · Hussein A. Zeineddine1 · Sithara Thomas1 · Shafeeque C. M.1 · Dania A. Jose1 · Kiara Torres1 · Jose Guzman1 · Andrew Dunn2,3 · P. KumarT.1 · Gadiparthi N. Rao4 · Spiros L. Blackburn1 ·  \nDevinW. McBride1  \nReceived: 23 May 2024 / Revised: 28 August 2024 / Accepted: 2 September 2024 / Published online: 19 September 2024 © The Author(s) 2024  \nAbstract  \nImpaired cerebral circulation, induced by blood vessel constrictions and microthrombi, leads to delayed cerebral ischemia after subarachnoid hemorrhage (SAH) . 12/15-Lipooxygenase (12/15-LOX) overexpression has been implicated in worsening early brain injury outcomes following SAH. However, it is unknown if 12/15-LOX is important in delayed pathophysiological events after SAH. Since 12/15-LOX produces metabolites that induce inflammation and vasoconstriction, we hypothesized that 12/15-LOX leads to microvessel constriction and microthrombi formation after SAH, and thus, 12/15-LOX is an important target to prevent delayed cerebral ischemia. SAH was induced in C57BL/6 and 12/15-LOX−/− mice of both sexes by endovascular perforation. Expression of 12/15-LOX was assessed in brain tissue slices and in vitro. C57BL/6 mice were administered either ML351 (12/15-LOX inhibitor) or vehicle. Mice were evaluated for daily neuroscore and euthanized on day 5 to assess cerebral 12/15-LOX expression, vessel constrictions, platelet activation, microthrombi, neurodegeneration, infarction, cortical perfusion, and development of delayed deficits. Finally, the effect of 12/15-LOX inhibition on platelet activation was assessed in SAH patient samples using a platelet spreading assay. In SAH mice, 12/15-LOX was upregulated in brain vascular cells, and there was an increase in 12-S-HETE. Inhibition of 12/15-LOX improved brain perfusion on days 4–5 and attenuated delayed pathophysiological events, including microvessel constrictions, microthrombi, neuronal degeneration, and infarction. Additionally, 12/15-LOX inhibition reduced platelet activation in human and mouse blood samples. Cerebrovascular 12/15-LOX overexpression plays a major role in brain dysfunction after SAH by triggering microvessel constrictions and microthrombi formation, which reduces brain perfusion. Inhibiting 12/15-LOX may be a therapeutic target to improve outcomes after SAH.  \nKeywords Subarachnoid hemorrhage · Delayed neurological deficit · Platelets · 12/15-Lipooxygenase · Microvessel constrictions · Microthrombi · Arterioles  \n* Ari Dienel[ari.c.dienel@uth.tmc.edu](ari.c.dienel@uth.tmc.edu)  \n* Devin W. McBride [devin.w.mcbride@uth.tmc.edu](devin.w.mcbride@uth.tmc.edu)  \n1 The Vivian L. Smith Department of Neurosurgery, McGovern Medical School, The University of Texas Health Science Center at Houston, Houston, TX 77459, USA  \n2 Department of Biomedical Engineering, The University of Texas at Austin, Austin, TX 78712, USA  \n3 Department of Electrical and Computer Engineering, The University of Texas at Austin, Austin, TX 78712, USA  \n4 Department of Physiology, University of Tennessee Health Science Center, Memphis, TN 38163, USA  \nIntroduction  \nSubarachnoid hemorrhage (SAH) affects about 30,000 individuals each year in the United States. Up to 30% of patients who survive aneurysm rupture develop delayed cerebral ischemia (DCI) 4 to 10 days following SAH, which accounts for the most common cause of morbidity and mortality in SAH survivors [1] . Multiple clinical studies indicate the cause of DCI is multifactorial and may include microthrombi, brain blood vessel constriction, and inflammation [2] .  \nAfter SAH, there is a significant enhancement in brain arachidonic acid metabolization, which triggers the formation of thrombi and vasospasm, worsen","cbCaitHT0zjMdrOE","https://ap.wps.com/l/cbCaitHT0zjMdrOE","pdf",7221255,17,"English","# Abstract\n# Introduction\n# Materials and Methods\n## Study Approval\n## Animal Study","[{\"question\":\"What delayed pathological effects were targeted after subarachnoid hemorrhage (SAH)?\",\"answer\":\"The study focused on delayed cerebral ischemia–related mechanisms, including microvessel constrictions, microthrombi formation, neuronal degeneration, and infarction.\"},{\"question\":\"How was 12/15-LOX inhibition tested in the mouse model?\",\"answer\":\"C57BL/6 mice were administered the 12/15-LOX inhibitor ML351 or vehicle after SAH, then evaluated over days for neuroscore, cerebral 12/15-LOX expression, vessel constrictions, platelet activation, microthrombi, perfusion, and delayed deficits.\"},{\"question\":\"Does 12/15-LOX inhibition affect platelet activation in human samples?\",\"answer\":\"Yes. The effect of 12/15-LOX inhibition on platelet activation was assessed in SAH patient samples using a platelet spreading assay, and inhibition reduced platelet activation in both human and mouse blood samples.\"}]","Translational Stroke Research - 12/15-Lipooxygenase Inhibition | PDF",1791282675,43]