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Drug toxicity and relapse drive the need for improved therapeutic strategies. The study builds a robust tumoroid-based platform that tests chemotherapeutic agents with focal therapy (thermotherapy) using clinically relevant trial protocols. Matrix-embedded tumoroids retain retinoblastoma characteristics and respond to repeated drug exposure in a manner resembling advanced clinical cases. A diode laser and online temperature monitoring reproduce thermotherapy and combined chemothermotherapy conditions, and model results match those obtained clinically for the key clinic drugs, supporting the platform’s translational value.",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":35,"@type":76,"position":81},"https://docshare.wps.com/document/healthcare/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/translational-screening-platform-to-evaluate-chemotherapy-in-combination-with-focal-therapy-for-retinoblastoma/381581/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/translational-screening-platform-to-evaluate-chemotherapy-in-combination-with-focal-therapy-for-retinoblastoma/381581.png","ImageObject",300,407,{"name":92,"@type":93},"Putri","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-10-30","2026-09-24",true,{"@type":102,"interactionType":103,"userInteractionCount":39},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"What problem does the study address in retinoblastoma treatment?","Question",{"text":112,"@type":113},"Retinoblastoma is treated with limited drug options adapted from other pediatric cancers, and toxicity and relapse create a need for new therapeutic strategies.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"How is the tumoroid-based screening platform designed?",{"text":117,"@type":113},"The model uses matrix-embedded tumoroids that preserve retinoblastoma features and respond to repeated chemotherapeutic drug exposure in ways intended to mirror advanced clinical cases.",{"name":119,"@type":110,"acceptedAnswer":120},"How does the platform replicate thermotherapy and combined treatments?",{"text":121,"@type":113},"A diode laser (810 nm) selectively heats the tumoroids, while an online system monitors intratumoral and surrounding temperatures to reproduce clinically relevant thermotherapy settings and combined chemothermotherapy protocols.","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},381581,1791471946,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":34,"category_name":35,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":39,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":139,"language":140,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":141,"faqs":142,"seo_title":143,"seo_description":67,"update_tm":144,"read_time":41},962085571259,"https://ap-avatar.wpscdn.com/davatar_29158cc5080c5b710cf443261637dec0","Received: 24 February 2023 | Revised: 15 May 2023 | Accepted: 20 May 2023  \nDOI: 10. 1111/cas.15878  \nOR I G I NAL ART I C L E  \nTranslational screening platform to evaluate chemotherapy in combination with focal therapy for retinoblastoma  \nIrina L. Sinenko1,2  | Fabien Kuttler3  | Valentin Simeonov4 | Alexandre Moulin2 | Patrick Aouad2 | Christina Stathopoulos2 | Francis L. Munier2 | Adeline Berger2  |  \nPaul J. Dyson1  \n1Institute of Chemical Sciences and Engineering, École Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland  \n2Ophthalmology Department, University of Lausanne, Jules-Gonin Eye Hospital, Fondation Asile des Aveugles, Lausanne, Switzerland  \n3 Biomolecular Screening Facility, School of Life Sciences, École Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland  \n4Laboratory of Environmental Remote Sensing, École Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland  \nCorrespondence  \nAdeline Berger, Ophthalmology Department, Jules-Gonin Eye Hospital, Fondation Asile des Aveugles, University of Lausanne, Avenue de France 15, CH- 1004 Lausanne, Switzerland.  \nEmail: [adeline.berger@fa2.ch](adeline.berger@fa2.ch)  \n[Paul J. Dyson](Paul J. Dyson), Institute of Chemical Sciences and Engineering, École Polytechnique Fédérale de Lausanne (EPFL), Avenue F.-A. Forel 2, CH-1015 Lausanne, Switzerland.  \nEmail: [paul.dyson@epfl.ch](paul.dyson@epfl.ch)  \nFunding information  \nH2020 Marie Skłodowska-Curie Actions, Grant/Award Number: 754354  \nAbstract  \nRetinoblastoma is the most common pediatric eye cancer. It is currently treated with a limited number of drugs, adapted from other pediatric cancer treatments. Drug toxicity and relapse of the disease warrant new therapeutic strategies for these young patients. In this study, we developed a robust tumoroid-based platform to test chemotherapeutic agents in combination with focal therapy (thermotherapy)– a treatment option widely used in clinical practice – in accordance with clinically relevant trial protocols. The model consists of matrix-embedded tumoroids that retain retinoblastoma features and respond to repeated chemotherapeutic drug exposure similarly to advanced clinical cases. Moreover, the screening platform includes a diode laser (810 nm, 0.3 W) to selectively heat the tumoroids, combined with an on-line system to monitor the intratumoral and surrounding temperatures. This allows the reproduction of the clinical settings of thermotherapy and combined chemothermotherapy treatments. When testing the two main drugs currently used in clinics to treat retinoblastoma in our model, we observed results similar to those clinically obtained, validating the utility of the model. This screening platform is the first system to accurately reproduce clinically relevant treatment methods and should lead to the identification of more efficient drugs to treat retinoblastoma.  \nK E Y WO R D S  \ncancer chemotherapy protocol, chemotherapy, chemothermotherapy, preclinical model, retinoblastoma, thermotherapy  \nAbbreviations: EGF, epidermal growth factor; FFPE, formalin-fixed-paraffin-embedded; FGF, fibroblast growth factor; GEMM, genetically-engineered mouse model; HA, hyaluronic acid; ICG, indocyanine green; LIF, leukemia inhibitory factor; PDX, patient-derived xenograft; RB1, retinoblastoma gene; TER, thermal enhancement ratio.  \nThis is an open access article under the terms of the Creative Commons Attribution-NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.  \n© 2023 The Authors. Cancer Science published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association.  \n1 | INTRODUCTION  \nRetinoblastoma is the most common intraocular malignancy of childhood that is typically diagnosed in infants under the age of 5 years.1 While the survival rate in high-income countries is higher than 95%, the relapse rate, incidence","cbCaibWH6kfr9MH0","https://ap.wps.com/l/cbCaibWH6kfr9MH0","pdf",19709141,12,"English","# Abstract\n# Introduction","[{\"question\":\"What problem does the study address in retinoblastoma treatment?\",\"answer\":\"Retinoblastoma is treated with limited drug options adapted from other pediatric cancers, and toxicity and relapse create a need for new therapeutic strategies.\"},{\"question\":\"How is the tumoroid-based screening platform designed?\",\"answer\":\"The model uses matrix-embedded tumoroids that preserve retinoblastoma features and respond to repeated chemotherapeutic drug exposure in ways intended to mirror advanced clinical cases.\"},{\"question\":\"How does the platform replicate thermotherapy and combined treatments?\",\"answer\":\"A diode laser (810 nm) selectively heats the tumoroids, while an online system monitors intratumoral and surrounding temperatures to reproduce clinically relevant thermotherapy settings and combined chemothermotherapy protocols.\"}]","Translational screening platform to evaluate chemotherapy in combination with focal therapy for retinoblastoma | PDF",1790246070]