[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"detail-sidebar-cat-0-en-105":3,"doc-seo-348988-105":59,"doc-detail-348988-en":129},{"code":4,"msg":5,"data":6},0,"success",[7,13,18,23,28,33,38,43,48,51,55],{"id":8,"doc_module":4,"doc_module_name":9,"category_name":10,"show_sort_weight":11,"slug":12},1,"Document","Story & Novel",90,"story-novel",{"id":14,"doc_module":4,"doc_module_name":9,"category_name":15,"show_sort_weight":16,"slug":17},2,"Literature",80,"literature",{"id":19,"doc_module":4,"doc_module_name":9,"category_name":20,"show_sort_weight":21,"slug":22},4,"Exam",70,"exam",{"id":24,"doc_module":4,"doc_module_name":9,"category_name":25,"show_sort_weight":26,"slug":27},5,"Comic",60,"comic",{"id":29,"doc_module":4,"doc_module_name":9,"category_name":30,"show_sort_weight":31,"slug":32},6,"Technology",50,"technology",{"id":34,"doc_module":4,"doc_module_name":9,"category_name":35,"show_sort_weight":36,"slug":37},7,"Healthcare",40,"healthcare",{"id":39,"doc_module":4,"doc_module_name":9,"category_name":40,"show_sort_weight":41,"slug":42},8,"Research & Report",30,"research-report",{"id":44,"doc_module":4,"doc_module_name":9,"category_name":45,"show_sort_weight":46,"slug":47},9,"Religion & Spirituality",20,"religion-spirituality",{"id":46,"doc_module":4,"doc_module_name":9,"category_name":49,"show_sort_weight":46,"slug":50},"World Cup","world-cup",{"id":52,"doc_module":4,"doc_module_name":9,"category_name":53,"show_sort_weight":52,"slug":54},10,"Lifestyle","lifestyle",{"id":56,"doc_module":4,"doc_module_name":9,"category_name":57,"show_sort_weight":24,"slug":58},19,"General","general",{"code":4,"msg":60,"data":61},"ok",{"site_id":62,"language":63,"slug":64,"title":65,"keywords":66,"description":67,"schema_data":68,"social_meta":122,"head_meta":124,"extra_data":126,"updated_unix":128},105,"en","transgelin-expression-in-activated-cancer-associated-fibroblasts-regulates-stromal-contractility-and-promotes-colon-cancer-progression","Transgelin Expression in Activated Cancer-Associated Fibroblasts Regulates Stromal Contractility and Promotes Colon Cancer Progression","","Transgelin is an actin-binding protein linked to cancer progression through activation of cancer-associated fibroblasts, and it has been proposed as a prognostic marker. The study examines transgelin’s distribution and function in colon cancer by analyzing transgelin expression in a tissue microarray of 359 colon cancer samples, and by testing stromal activation using knockdown and co-transplantation models to assess tumor growth and stromal contractility.",{"@graph":69,"@context":121},[70,84,104],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":40,"@type":76,"position":81},"https://docshare.wps.com/document/research-report/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/transgelin-expression-in-activated-cancer-associated-fibroblasts-regulates-stromal-contractility-and-promotes-colon-cancer-progression/348988/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":98,"encodingFormat":97,"isAccessibleForFree":99,"interactionStatistic":100},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/transgelin-expression-in-activated-cancer-associated-fibroblasts-regulates-stromal-contractility-and-promotes-colon-cancer-progression/348988.png","ImageObject",300,407,{"name":92,"@type":93},"Aria Callaghan","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-09-22",true,{"@type":101,"interactionType":102,"userInteractionCount":4},"InteractionCounter",{"@type":103},"ViewAction",{"@type":105,"mainEntity":106},"FAQPage",[107,113,117],{"name":108,"@type":109,"acceptedAnswer":110},"What is the main goal of this study on transgelin in colon cancer?","Question",{"text":111,"@type":112},"To clarify the mechanistic role of transgelin in colon cancer progression, with emphasis on its functional impact in cancer-associated fibroblasts.","Answer",{"name":114,"@type":109,"acceptedAnswer":115},"How was transgelin expression assessed in human colon cancer tissues?",{"text":116,"@type":112},"Tissue microarrays from 359 human colon cancer samples were used to evaluate the clinical relevance and stromal distribution of transgelin expression.",{"name":118,"@type":109,"acceptedAnswer":119},"What effect does fibroblast transgelin knockdown have on colon cancer outcomes in the models described?",{"text":120,"@type":112},"Fibroblast transgelin knockdown suppresses collagen gel contractility and inhibits tumor growth in co-transplantation experiments in immunodeficient mice.","https://schema.org",{"og:url":83,"og:type":123,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":125,"canonical":83},"index,follow",{"doc_id":127,"site_id":62},348988,1790081036,{"code":4,"msg":5,"data":130},{"doc_id":127,"user_id":131,"nickname":92,"user_avatar":132,"doc_module":4,"category_id":39,"category_name":40,"doc_title":65,"doc_description":67,"doc_content":133,"file_id":134,"file_url":135,"file_type":136,"file_size":137,"view_count":4,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":138,"language":139,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":140,"faqs":141,"seo_title":142,"seo_description":67,"update_tm":128,"read_time":41},962084926284,"https://ap-avatar.wpscdn.com/davatar_29158cc5080c5b710cf443261637dec0","Cancer Medicine  \n|  RESEARCH ARTICLE  OPEN ACCESS \u003Cbr>Transgelin Expression in Activated Cancer-Associated Fibroblasts Regulates Stromal Contractility and Promotes Colon Cancer Progression\u003Cbr>Ryuji Okamoto1,2,3  | Tetsuro Hiroi1 | Yuka Nakamura4 | Hiroko Hashimoto4 | Shingo Sakashita4  | Naoya Sakamoto4 | Shumpei Ishikawa4,5 | Horacio Cabral6 | Triantafyllos Stylianopoulos7 | Yuichiro Tsukada8 | Atsushi Ochiai9 |\u003Cbr>Tomonori Yano2,3 | Masaaki Ito8  | Genichiro Ishii1,3,4  | Motohiro Kojima1,10,11\u003Cbr>1Department of Pathology and Clinical Laboratories, National Cancer Center Hospital East, Kashiwa, Japan | 2Department of Gastroenterology and Endoscopy, National Cancer Center Hospital East, Kashiwa, Japan | 3Course of Advanced Clinical Research, Cancer Juntendo University Graduate School of Medicine, Tokyo, Japan | 4Division of Innovative Pathology and Laboratory Medicine, National Cancer Center Exploratory Oncology Research and Clinical Trial Center, Kashiwa, Japan | 5Department of Preventive Medicine, Graduate School of Medicine, The University of Tokyo, Tokyo,\u003Cbr>Japan | 6Department of Bioengineering, Graduate School of Engineering, The University of Tokyo, Tokyo, Japan | 7Cancer Biophysics Laboratory, Department of Mechanical and Manufacturing Engineering, University of Cyprus, Nicosia, Cyprus | 8Department of Colorectal Surgery, National Cancer Center Hospital East, Kashiwa, Japan | 9Research Institute for Biomedical Sciences, Tokyo University of Science, Tokyo, Japan | 10Division of Pathology, National Cancer Center Exploratory Oncology Research and Clinical Trial Center, Kashiwa, Japan | 11Department of Surgical Pathology, Kyoto Prefectural University of Medicine, Kyoto, Japan\u003Cbr>Correspondence: Motohiro Kojima ([mokojima@koto.kpu-m.ac.jp](mokojima@koto.kpu-m.ac.jp))\u003Cbr>Received: 9 November 2025 | Revised: 10 February 2026 | Accepted: 27 March 2026\u003Cbr>Keywords: colon cancer | fibroblasts | transgelin |\n| --- |\n| ABSTRACT\u003Cbr>Transgelin is an actin-binding protein that promotes cancer progression via activation of cancer-associated fibroblasts and has been identified as a prognostic marker. However, its distribution and functional role in colon cancer remain unclear. In this study, we aimed to elucidate the mechanistic role of transgelin in colon cancer progression by focusing on its functional impact in cancer-associated fibroblasts. Tissue microarrays from 359 human colon cancer tissues were investigated to elucidate the clinical importance of transgelin expression in cancer stroma. We focused on transgelin in fibroblasts and investigated its functional role in stromal activation using in vitro knockdown experiments and in vivo co-transplantation models. Primary cultures of human colon fibroblasts were evaluated for their biological function. Our data showed that transgelin expression is predominant in activated cancer-associated fibroblasts in colon cancer tissues. Stimulation by cancer-cell-conditioned medium (CM) significantly upregulated transgelin, ACTA2, COL1A1, and TNC expression in colonic fibroblasts. Additionally, transgelin knockdown (KD) in fibroblasts did not influence the upregulation except for transgelin itself. Transgelin KD in fibroblasts did not result in drastic alterations in gene expression profiles. Transgelin KD suppressed collagen gel contractility. Furthermore, co-transplantation experiments of cancer cells and colonic fibroblasts into immunodeficient mice revealed that transgelin KD inhibited tumor growth in fibroblasts. In conclusion, stromal transgelin expression in colon cancer strongly correlated with distant metastasis and served as a prognostic factor for colon cancer.\u003Cbr>Mechanistically, transgelin in cancer-associated fibroblasts promotes tumor growth by regulating stromal contractility, suggesting transgelin as a potential therapeutic target. |\n|  |\n| Abbreviations: CAFs, cancer-associated fibroblasts; CM, conditioned medium; cSPFs, colonic subperitoneal fibroblasts; DEG, differentially","cbCaidCKpZ40kNMv","https://ap.wps.com/l/cbCaidCKpZ40kNMv","pdf",2390329,12,"English","# ABSTRACT\n## Introduction","[{\"question\":\"What is the main goal of this study on transgelin in colon cancer?\",\"answer\":\"To clarify the mechanistic role of transgelin in colon cancer progression, with emphasis on its functional impact in cancer-associated fibroblasts.\"},{\"question\":\"How was transgelin expression assessed in human colon cancer tissues?\",\"answer\":\"Tissue microarrays from 359 human colon cancer samples were used to evaluate the clinical relevance and stromal distribution of transgelin expression.\"},{\"question\":\"What effect does fibroblast transgelin knockdown have on colon cancer outcomes in the models described?\",\"answer\":\"Fibroblast transgelin knockdown suppresses collagen gel contractility and inhibits tumor growth in co-transplantation experiments in immunodeficient mice.\"}]","Transgelin Expression in Activated Cancer-Associated Fibroblasts Regulates Stromal Contractility and Promotes Colon Cancer Progression | PDF"]