[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"detail-sidebar-cat-0-en-105":3,"doc-seo-450225-105":59,"doc-detail-450225-en":130},{"code":4,"msg":5,"data":6},0,"success",[7,13,18,23,28,33,38,43,48,51,55],{"id":8,"doc_module":4,"doc_module_name":9,"category_name":10,"show_sort_weight":11,"slug":12},1,"Document","Story & Novel",90,"story-novel",{"id":14,"doc_module":4,"doc_module_name":9,"category_name":15,"show_sort_weight":16,"slug":17},2,"Literature",80,"literature",{"id":19,"doc_module":4,"doc_module_name":9,"category_name":20,"show_sort_weight":21,"slug":22},4,"Exam",70,"exam",{"id":24,"doc_module":4,"doc_module_name":9,"category_name":25,"show_sort_weight":26,"slug":27},5,"Comic",60,"comic",{"id":29,"doc_module":4,"doc_module_name":9,"category_name":30,"show_sort_weight":31,"slug":32},6,"Technology",50,"technology",{"id":34,"doc_module":4,"doc_module_name":9,"category_name":35,"show_sort_weight":36,"slug":37},7,"Healthcare",40,"healthcare",{"id":39,"doc_module":4,"doc_module_name":9,"category_name":40,"show_sort_weight":41,"slug":42},8,"Research & Report",30,"research-report",{"id":44,"doc_module":4,"doc_module_name":9,"category_name":45,"show_sort_weight":46,"slug":47},9,"Religion & Spirituality",20,"religion-spirituality",{"id":46,"doc_module":4,"doc_module_name":9,"category_name":49,"show_sort_weight":46,"slug":50},"World Cup","world-cup",{"id":52,"doc_module":4,"doc_module_name":9,"category_name":53,"show_sort_weight":52,"slug":54},10,"Lifestyle","lifestyle",{"id":56,"doc_module":4,"doc_module_name":9,"category_name":57,"show_sort_weight":24,"slug":58},19,"General","general",{"code":4,"msg":60,"data":61},"ok",{"site_id":62,"language":63,"slug":64,"title":65,"keywords":66,"description":67,"schema_data":68,"social_meta":123,"head_meta":125,"extra_data":127,"updated_unix":129},105,"en","transcriptomic-insights-into-gsdme-driven-pyroptosis-in-head-and-neck-cancer","Transcriptomic insights into GSDME-driven pyroptosis in head and neck cancer","","Investigating pyroptosis-related genes (PRGs) in head and neck squamous cell carcinoma (HNSCC), the study evaluates their expression and links to the tumor immune microenvironment. Curated TCGA-HNSCC and GSE65858 datasets were used to identify pyroptosis-associated differentially expressed genes, followed by KEGG and GO enrichment analyses and CIBERSORTx-based assessment of 22 immune cell types. Using Illumina NovaSeq 6000 sequencing, plus immunohistochemistry validation, the work identifies mutations among PRGs and highlights GSDME-associated changes in CK13 and CK19, implicating a potential mechanism affecting HNSCC growth.",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":40,"@type":76,"position":81},"https://docshare.wps.com/document/research-report/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/transcriptomic-insights-into-gsdme-driven-pyroptosis-in-head-and-neck-cancer/450225/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/transcriptomic-insights-into-gsdme-driven-pyroptosis-in-head-and-neck-cancer/450225.png","ImageObject",300,407,{"name":92,"@type":93},"Theodore","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-10-08","2026-09-30",true,{"@type":102,"interactionType":103,"userInteractionCount":24},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"What datasets were used to analyze pyroptosis-related genes in HNSCC?","Question",{"text":112,"@type":113},"The analysis used curated TCGA-HNSCC and GSE65858 datasets, with TCGA patient data and tumor samples from GSE65858.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"How was immune cell infiltration assessed in the study?",{"text":117,"@type":113},"Immune infiltration across 22 immune cell types was assessed using the CIBERSORTx algorithm.",{"name":119,"@type":110,"acceptedAnswer":120},"Which markers were found to change in the GSDME-overexpressing group?",{"text":121,"@type":113},"Transcriptomic and immunohistochemistry results showed a significant decrease in CK13 (KRT13) and CK19 (KRT19) protein expression in the GSDME-overexpressing group.","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},450225,1790813836,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":39,"category_name":40,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":24,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":139,"language":140,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":141,"faqs":142,"seo_title":143,"seo_description":67,"update_tm":144,"read_time":145},7971461740886,"https://ap-avatar.wpscdn.com/davatar_3d24733baf745e90a7e4bdd5f77d97b2","[www. nature.com/scientificreports](www. nature.com/scientificreports)  \nOPEN  \nTranscriptomic insights into GSDME-driven pyroptosis in head and neck cancer  \nFengxiang Hao1,2, Danlei Qin1, Ran Li2, Xia Li2, Jiayi Hou3 & Bin Zhao1,2􀀍  \nThis study investigates the expression of pyroptosis-related genes (PRGs) in head and neck squamous cell carcinoma (HNSCC) and their potential role in the tumor immune microenvironment. Additionally, we conducted an in-depth transcriptomic analysis of HNSCC with high GSDME expression. This study utilized the curated TCGA-HNSCC and GSE65858 datasets to analyze differentially expressed genes (DEGs) associated with pyroptosis in HNSCC. Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO) enrichment analyses were performed on these DEGs. The infiltration of  \n22 immune cell types was assessed using the CIBERSORTx algorithm. GSDME-overexpressing cells were sequenced using the Illumina NovaSeq 6000 platform, and immunohistochemistry (IHC) was conducted for validation. A total of 501 TCGA-HNSCC patient data and 270 tumor samples from the GSE65858 dataset were collected. Seventeen pyroptosis-related genes were found to have mutations in HNSCC samples, with TP53, CASP8, and NLRP3 exhibiting the highest mutation frequencies. GO enrichment analysis revealed that the DEGs were enriched in cellular components such as myofibrilsand collagen-containing extracellular matrix, as well as in molecular functions including actin binding and receptor ligand activity. KEGG pathway enrichment analysis showed that DEGs were enriched in the focal adhesion biological pathway. GSEA analysis indicated a negative correlation between DEGs and the cytochrome P450 pathway. Transcriptomic analysis of high GSDME expression revealed a significant decrease inCK13 (KRT13) and CK19 (KRT19) in the GSDME-overexpressing group. Downregulated genes were significantly enriched in cell adhesion molecules and the arachidonic acid pathway. Immunohistochemistry confirmed a statistically significant reduction in CK13 and CK19 protein expression in the high GSDME expression group. Transcriptomic analysis of GSDME overexpression showed significant upregulation or downregulation of transcription factor families, including E2F, ETS, HMG, MYB, BZIP, and C2H2. Specifically, ELF3 from the ETS transcription factor family and PBX1 from the Homeobox transcription factor family were downregulated. GSDME is potentially associated with the ETS transcription factor ELF3 and the Homeobox transcription factor PBX1. The reduced expression of CK13 and CK19 in the GSDME-overexpressing group may serve as a potential mechanism by which GSDME inhibits HNSCC growth.  \nKeywords Pyroptosis, Head and neck squamous cell carcinoma, Immune environment, GSDME, CK13, CK19  \nAbbreviations  \nHNSCC TGCA  \nGEO  \nPRGLASSOTME  \nGSDMRNA-seq FDR  \nGO  \nHead and neck squamous cell carcinoma The cancer genome atlas  \nGene expression omnibus  \nPyroptosis-related gene  \nLeast absolute shrinkage and selection operator Tumour microenvironment  \nGasdermin  \nRNA sequencing  \nFalse discovery rate  \nGene ontology  \n1Shanxi Medical University, Taiyuan 030001, Shanxi Province, China. 2Shanxi Medical University School and Hospital of Stomatology, Shanxi Province Key Laboratory of Oral Diseases Prevention and New Materials, Taiyuan 030001, Shanxi Province, China. 3Department of Clinical Laboratory, Shanxi Provincial Academy of Traditional Chinese Medicine, Taiyuan, Shanxi Province, China. 􀀍 email: [sxmu0688@126.com](sxmu0688@126.com)  \n[www. nature.com/scientificreports/](www. nature.com/scientificreports/)  \nKEGG Kyoto encyclopedia of genes and genomes ssGSEA Single-sample gene-set enrichment analysis  \nHead and neck squamous cell carcinoma (HNSCC) is a globally widespread malignant tumour that commonly occurs in the oral cavity, sinonasal cavity, pharynx, and larynx1,2. It has a low 5-year survival rate and high rates of recurrence and metastasis3. It is estimated that in 2022,","cbCaioKn0O9i86lx","https://ap.wps.com/l/cbCaioKn0O9i86lx","pdf",2960211,13,"English","# Transcriptomic insights into GSDME-driven pyroptosis in head and neck cancer\n## Pyroptosis and its dual role in cancer\n## Study design: datasets, DEG analysis, enrichment, and immune infiltration\n## GSDME-high expression findings and validation","[{\"question\":\"What datasets were used to analyze pyroptosis-related genes in HNSCC?\",\"answer\":\"The analysis used curated TCGA-HNSCC and GSE65858 datasets, with TCGA patient data and tumor samples from GSE65858.\"},{\"question\":\"How was immune cell infiltration assessed in the study?\",\"answer\":\"Immune infiltration across 22 immune cell types was assessed using the CIBERSORTx algorithm.\"},{\"question\":\"Which markers were found to change in the GSDME-overexpressing group?\",\"answer\":\"Transcriptomic and immunohistochemistry results showed a significant decrease in CK13 (KRT13) and CK19 (KRT19) protein expression in the GSDME-overexpressing group.\"}]","Transcriptomic insights into GSDME-driven pyroptosis in head and neck cancer | PDF",1790732535,33]