[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"detail-sidebar-cat-0-en-105":3,"doc-seo-340086-105":59,"doc-detail-340086-en":131},{"code":4,"msg":5,"data":6},0,"success",[7,13,18,23,28,33,38,43,48,51,55],{"id":8,"doc_module":4,"doc_module_name":9,"category_name":10,"show_sort_weight":11,"slug":12},1,"Document","Story & Novel",90,"story-novel",{"id":14,"doc_module":4,"doc_module_name":9,"category_name":15,"show_sort_weight":16,"slug":17},2,"Literature",80,"literature",{"id":19,"doc_module":4,"doc_module_name":9,"category_name":20,"show_sort_weight":21,"slug":22},4,"Exam",70,"exam",{"id":24,"doc_module":4,"doc_module_name":9,"category_name":25,"show_sort_weight":26,"slug":27},5,"Comic",60,"comic",{"id":29,"doc_module":4,"doc_module_name":9,"category_name":30,"show_sort_weight":31,"slug":32},6,"Technology",50,"technology",{"id":34,"doc_module":4,"doc_module_name":9,"category_name":35,"show_sort_weight":36,"slug":37},7,"Healthcare",40,"healthcare",{"id":39,"doc_module":4,"doc_module_name":9,"category_name":40,"show_sort_weight":41,"slug":42},8,"Research & Report",30,"research-report",{"id":44,"doc_module":4,"doc_module_name":9,"category_name":45,"show_sort_weight":46,"slug":47},9,"Religion & Spirituality",20,"religion-spirituality",{"id":46,"doc_module":4,"doc_module_name":9,"category_name":49,"show_sort_weight":46,"slug":50},"World Cup","world-cup",{"id":52,"doc_module":4,"doc_module_name":9,"category_name":53,"show_sort_weight":52,"slug":54},10,"Lifestyle","lifestyle",{"id":56,"doc_module":4,"doc_module_name":9,"category_name":57,"show_sort_weight":24,"slug":58},19,"General","general",{"code":4,"msg":60,"data":61},"ok",{"site_id":62,"language":63,"slug":64,"title":65,"keywords":66,"description":67,"schema_data":68,"social_meta":124,"head_meta":126,"extra_data":128,"updated_unix":130},105,"en","thiostrepton-as-a-potential-therapeutic-agent-for-hepatocellular-carcinoma-read-online-free","Thiostrepton as a Potential Therapeutic Agent for Hepatocellular Carcinoma - read online free","","Limited drug efficacy and drug resistance make it urgent to discover effective anti-liver cancer therapies. This study applies a synergistic computational and experimental strategy to repurpose thiostrepton (TST) as a candidate treatment for hepatocellular carcinoma (HCC). TST inhibits HCC cell proliferation, induces cell-cycle arrest and apoptosis, and suppresses cell migration. Mechanistically, TST triggers mitochondrial damage, increases reactive oxygen species (ROS), and activates mitophagy; ROS scavenging by NAC and autophagy inhibition respectively reverse or enhance anti-cancer effects. Overall, the work supports TST as a promising option for liver cancer therapy.",{"@graph":69,"@context":123},[70,84,106],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":35,"@type":76,"position":81},"https://docshare.wps.com/document/healthcare/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/thiostrepton-as-a-potential-therapeutic-agent-for-hepatocellular-carcinoma-read-online-free/340086/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/thiostrepton-as-a-potential-therapeutic-agent-for-hepatocellular-carcinoma-read-online-free/340086.png","ImageObject",300,407,{"name":92,"@type":93},"Miles","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-10-27","2026-09-21",true,{"@type":102,"interactionType":103,"userInteractionCount":105},"InteractionCounter",{"@type":104},"ViewAction",13,{"@type":107,"mainEntity":108},"FAQPage",[109,115,119],{"name":110,"@type":111,"acceptedAnswer":112},"Why is drug repurposing important for hepatocellular carcinoma (HCC)?","Question",{"text":113,"@type":114},"Drug repurposing is described as efficient because it can reduce costs, shorten development cycles, and provide assured safety compared with developing new drugs from scratch.","Answer",{"name":116,"@type":111,"acceptedAnswer":117},"What effects does thiostrepton (TST) have on HCC cells?",{"text":118,"@type":114},"TST inhibits HCC cell proliferation, arrests the cell cycle, induces apoptosis, and suppresses cell migration.",{"name":120,"@type":111,"acceptedAnswer":121},"How does TST exert its anti-HCC mechanism?",{"text":122,"@type":114},"TST impairs mitochondria, increases reactive oxygen species (ROS), and induces mitophagy; ROS scavenging with NAC restores proliferation and mitochondrial function, while autophagy inhibition enhances TST’s anti-cancer effects.","https://schema.org",{"og:url":83,"og:type":125,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":127,"canonical":83},"index,follow",{"doc_id":129,"site_id":62},340086,1790077578,{"code":4,"msg":5,"data":132},{"doc_id":129,"user_id":133,"nickname":92,"user_avatar":134,"doc_module":4,"category_id":34,"category_name":35,"doc_title":65,"doc_description":67,"doc_content":135,"file_id":136,"file_url":137,"file_type":138,"file_size":139,"view_count":105,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":140,"language":141,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":142,"faqs":143,"seo_title":144,"seo_description":67,"update_tm":145,"read_time":146},13056703019404,"https://ap-avatar.wpscdn.com/davatar_29158cc5080c5b710cf443261637dec0","Article  \nThiostrepton as a Potential Therapeutic Agent for Hepatocellular Carcinoma  \nGuifeng Su 1,2,3,4, Qianqing Yang 1, Heyang Zhou 1, Ying Huang 1, Shiyun Nie 1, Dan Wang 1, Guangchao Ma 1, Shaohua Zhang 1, Lingmei Kong 1, Chenggang Zou 2, * and Yan Li 1,2,3, *  \nCitation: Su, G.; Yang, Q.; Zhou, H.; Huang, Y.; Nie, S.; Wang, D.; Ma, G.; Zhang, S.; Kong, L.; Zou, C.; et al. Thiostrepton as a Potential Therapeutic Agent for Hepatocellular Carcinoma. Int. J. Mol. Sci. 2024, 25, 9717. [https://doi.org/10.3390/](https://doi.org/10.3390/)[ ](https://doi.org/10.3390/)ijms25179717  \nAcademic Editor: Nam Deuk Kim  \nReceived: 29 July 2024  \nRevised: 2 September 2024  \nAccepted: 3 September 2024  \nPublished: 8 September 2024  \nCopyright: © 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license ([https://](https://)[ ](https://)[creativecommons.org/licenses/by/](creativecommons.org/licenses/by/)[ ](creativecommons.org/licenses/by/)[4.0/](4.0/)) .  \n1 Key Laboratory of Medicinal Chemistry for Natural Resource, Ministry of Education; Yunnan Key Laboratory of Research and Development for Natural Products, School of Pharmacy, Yunnan University, Kunming 650500, China; [suguifeng@mail.kib.ac.cn](suguifeng@mail.kib.ac.cn) (G.S.); [yangqianqing@mail.ynu.edu.cn](yangqianqing@mail.ynu.edu.cn) (Q.Y.); [hyzhou@mail.ynu.edu.cn](hyzhou@mail.ynu.edu.cn) (H.Z.); [huangying@mail.yun.edu.cn](huangying@mail.yun.edu.cn) (Y.H.); [nieshiyun@mail.ynu.edu.cn](nieshiyun@mail.ynu.edu.cn) (S.N.); [wangdan@mail.ynu.edu.cn](wangdan@mail.ynu.edu.cn) (D.W.); [guangchaoma@mail.ynu.edu.cn](guangchaoma@mail.ynu.edu.cn) (G.M.); [shzhang_cpu@126.com](shzhang_cpu@126.com) (S.Z.); [konglingmei@ynu.edu.cn](konglingmei@ynu.edu.cn) (L.K.)  \n2 State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, School of Life Sciences, Yunnan University, Kunming 650500, China  \n3 State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China  \n4 University of Chinese Academy of Sciences, Beijing 100049, China  \n* Correspondence: [chgzou@ynu.edu.cn](chgzou@ynu.edu.cn) (C.Z.); [yan.li@ynu.edu.cn](yan.li@ynu.edu.cn) (Y.L.); Tel.: +86-871-65936165 (Y.L.)  \nAbstract: Due to limited drug efficacy and drug resistance, it is urgent to explore effective anti-liver cancer drugs. Repurposing drugs is an efficient strategy, with advantages including reduced costs, shortened development cycles, and assured safety. In this study, we adopted a synergistic approach combining computational and experimental methods and identified the antibacterial drug thiostrepton (TST) as a candidate for an anti-liver cancer drug. Although the anti-tumor capabilities of TST have been reported, its role and underlying mechanisms in hepatocellular carcinoma (HCC) remain unclear. TST was found here to inhibit the proliferation of HCC cells effectively, arresting the cell cycle and inducing cell apoptosis, as well as suppressing the cell migration. Further, our findings revealed that TST induced mitochondrial impairment, which was demonstrated by destroyed mitochondrial structures, reduced mitochondria, and decreased mitochondrial membrane potential (MMP) . TST caused the production of reactive oxygen species (ROS), and the mitochondrial impairment and proliferation inhibition of HCC cells were completely restored by the ROS scavenger N-acetyl-L-cysteine (NAC). Moreover, we discovered that TST induced mitophagy, and autophagy inhibition effectively promoted the anti-cancer effects of TST on HCC cells. In conclusion, our study suggests TST as a promising candidate for the treatment of liver cancers, and these findings provide theoretical support for the further development and potential application of TST in clinical liver cancer therapy.  \nKeywords: thiostrepton; hepatocellul","cbCaiqOeJkmYdq4p","https://ap.wps.com/l/cbCaiqOeJkmYdq4p","pdf",8636708,15,"English","# Abstract\n# Keywords\n# Introduction","[{\"question\":\"Why is drug repurposing important for hepatocellular carcinoma (HCC)?\",\"answer\":\"Drug repurposing is described as efficient because it can reduce costs, shorten development cycles, and provide assured safety compared with developing new drugs from scratch.\"},{\"question\":\"What effects does thiostrepton (TST) have on HCC cells?\",\"answer\":\"TST inhibits HCC cell proliferation, arrests the cell cycle, induces apoptosis, and suppresses cell migration.\"},{\"question\":\"How does TST exert its anti-HCC mechanism?\",\"answer\":\"TST impairs mitochondria, increases reactive oxygen species (ROS), and induces mitophagy; ROS scavenging with NAC restores proliferation and mitochondrial function, while autophagy inhibition enhances TST’s anti-cancer effects.\"}]","Thiostrepton as a Potential Therapeutic Agent for Hepatocellular Carcinoma - read online free | PDF",1790034125,38]