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This study used in silico and in vitro analyses to define the miR-145-5p–EMT axis in prostate cancer, identifying MYO6 as a validated target. miR-145-5p inhibition or overexpression affected proliferation, clonogenicity, migration, EMT marker expression, and suggested recurrence prediction.",{"@graph":14,"@context":73},[15,34,56],{"@type":16,"itemListElement":17},"BreadcrumbList",[18,23,27,31],{"item":19,"name":20,"@type":21,"position":22},"https://docshare.wps.com","Home","ListItem",1,{"item":24,"name":25,"@type":21,"position":26},"https://docshare.wps.com/document/","Document",2,{"item":28,"name":29,"@type":21,"position":30},"https://docshare.wps.com/document/research-report/","Research & Report",3,{"item":32,"name":10,"@type":21,"position":33},"https://docshare.wps.com/document/the-suppression-of-the-epithelial-to-mesenchymal-transition-in-prostate-cancer-through-the-targeting-of-myo6-using-mir-145-5p/332019/",4,{"url":32,"name":10,"@type":35,"image":36,"author":41,"headline":10,"publisher":44,"fileFormat":47,"inLanguage":8,"description":12,"dateModified":48,"datePublished":49,"encodingFormat":47,"isAccessibleForFree":50,"interactionStatistic":51},"DigitalDocument",{"url":37,"@type":38,"width":39,"height":40},"https://docshare.wps.com/thumbnails/the-suppression-of-the-epithelial-to-mesenchymal-transition-in-prostate-cancer-through-the-targeting-of-myo6-using-mir-145-5p/332019.png","ImageObject",300,407,{"name":42,"@type":43},"Ava Thompson","Person",{"url":19,"name":45,"@type":46},"DocShare","Organization","application/pdf","2026-11-13","2026-09-21",true,{"@type":52,"interactionType":53,"userInteractionCount":55},"InteractionCounter",{"@type":54},"ViewAction",13,{"@type":57,"mainEntity":58},"FAQPage",[59,65,69],{"name":60,"@type":61,"acceptedAnswer":62},"What is the main biological process investigated in this article?","Question",{"text":63,"@type":64},"The article focuses on epithelial-to-mesenchymal transition (EMT), which supports prostate cancer progression and metastasis.","Answer",{"name":66,"@type":61,"acceptedAnswer":67},"How does miR-145-5p relate to EMT in prostate cancer?",{"text":68,"@type":64},"The study tests whether miR-145-5p can suppress EMT in prostate cancer and explores the underlying molecular interaction.",{"name":70,"@type":61,"acceptedAnswer":71},"Which gene is identified as a target of miR-145-5p?",{"text":72,"@type":64},"MYO6 is identified and validated as a novel target of miR-145-5p in prostate cancer cells.","https://schema.org",{"og:url":32,"og:type":75,"og:title":10,"og:site_name":45,"og:description":12},"article",{"robots":77,"canonical":32},"index,follow",{"doc_id":79,"site_id":7},332019,1790125734,{"code":4,"msg":82,"data":83},"success",[84,88,92,96,101,106,111,115,120,123,127],{"id":22,"doc_module":4,"doc_module_name":25,"category_name":85,"show_sort_weight":86,"slug":87},"Story & Novel",90,"story-novel",{"id":26,"doc_module":4,"doc_module_name":25,"category_name":89,"show_sort_weight":90,"slug":91},"Literature",80,"literature",{"id":33,"doc_module":4,"doc_module_name":25,"category_name":93,"show_sort_weight":94,"slug":95},"Exam",70,"exam",{"id":97,"doc_module":4,"doc_module_name":25,"category_name":98,"show_sort_weight":99,"slug":100},5,"Comic",60,"comic",{"id":102,"doc_module":4,"doc_module_name":25,"category_name":103,"show_sort_weight":104,"slug":105},6,"Technology",50,"technology",{"id":107,"doc_module":4,"doc_module_name":25,"category_name":108,"show_sort_weight":109,"slug":110},7,"Healthcare",40,"healthcare",{"id":112,"doc_module":4,"doc_module_name":25,"category_name":29,"show_sort_weight":113,"slug":114},8,30,"research-report",{"id":116,"doc_module":4,"doc_module_name":25,"category_name":117,"show_sort_weight":118,"slug":119},9,"Religion & Spirituality",20,"religion-spirituality",{"id":118,"doc_module":4,"doc_module_name":25,"category_name":121,"show_sort_weight":118,"slug":122},"World Cup","world-cup",{"id":124,"doc_module":4,"doc_module_name":25,"category_name":125,"show_sort_weight":124,"slug":126},10,"Lifestyle","lifestyle",{"id":128,"doc_module":4,"doc_module_name":25,"category_name":129,"show_sort_weight":97,"slug":130},19,"General","general",{"code":4,"msg":82,"data":132},{"doc_id":79,"user_id":133,"nickname":42,"user_avatar":134,"doc_module":4,"category_id":112,"category_name":29,"doc_title":10,"doc_description":12,"doc_content":135,"file_id":136,"file_url":137,"file_type":138,"file_size":139,"view_count":55,"is_deleted":4,"is_public":22,"is_downloadable":22,"audit_status":22,"page_count":118,"language":140,"language_code":8,"site_id":7,"html_lang":8,"table_of_contents":141,"faqs":142,"seo_title":143,"seo_description":12,"update_tm":144,"read_time":104,"preview_page_count":4},1649267921044,"https://us-avatar.wpscdn.com/avatar/1800007509477c92dfb?_k=1786009248482753345","Article  \nThe Suppression of the Epithelial to Mesenchymal Transition in Prostate Cancer through the Targeting of MYO6 Using MiR-145-5p  \nLee Armstrong, Colin E. Willoughby  and Declan J. McKenna *  \nCitation: Armstrong, L.; Willoughby, C.E.; McKenna, D.J. The Suppression of the Epithelial to Mesenchymal Transition in Prostate Cancer through the Targeting of MYO6 Using MiR-145-5p. Int. J. Mol. Sci. 2024, 25, 4301. [https://doi.org/10.3390/](https://doi.org/10.3390/)[ ](https://doi.org/10.3390/)ijms25084301  \nAcademic Editor: Kexin Xu  \nReceived: 19 March 2024  \nRevised: 9 April 2024  \nAccepted: 11 April 2024  \nPublished: 12 April 2024  \nCopyright: © 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license ([https://](https://)[ ](https://)[creativecommons.org/licenses/by/](creativecommons.org/licenses/by/)[ ](creativecommons.org/licenses/by/)[4.0/](4.0/)) .  \nGenomic Medicine Research Group, Ulster University, Cromore Road, Coleraine BT52 1SA, UK;  \n[armstrong-l16@ulster.ac.uk](armstrong-l16@ulster.ac.uk) (L.A.); [c.willoughby@ulster.ac.uk](c.willoughby@ulster.ac.uk) (C.E.W.)  \n* [Correspondence: dj.mckenna@ulster.ac.uk](Correspondence: dj.mckenna@ulster.ac.uk)  \nAbstract: Aberrant expression of miR-145-5p has been observed in prostate cancer where is has been suggested to play a tumor suppressor role. In other cancers, miR-145-5p acts as an inhibitor of epithelial-to-mesenchymal transition (EMT), a key molecular process for tumor progression. However, the interaction between miR-145-5p and EMT remains to be elucidated in prostate cancer. In this paper the link between miR-145-5p and EMT in prostate cancer was investigated using a combination of in silico and in vitro analyses. miR-145-5p expression was significantly lower in prostate cancer cell lines compared to normal prostate cells. Bioinformatic analysis of The Cancer Genome Atlas prostate adenocarcinoma (TCGA PRAD) data showed significant downregulation of miR-145-5p in prostate cancer, correlating with disease progression. Functional enrichment analysis significantly associated miR-145-5p and its target genes with EMT. MYO6, an EMT-associated gene, was identified and validated as a novel target of miR-145-5p in prostate cancer cells. In vitro manipulation of miR-145-5p levels significantly altered cell proliferation, clonogenicity, migration and expression of EMT-associated markers. Additional TCGA PRAD analysis suggested miR-145-5p tumor expression may be useful predictor of disease recurrence. In summary, this is the first study to report that miR-145-5p may inhibit EMT by targeting MYO6 in prostate cancer cells. The findings suggest miR-145-5p could be a useful diagnostic and prognostic biomarker for prostate cancer.  \nKeywords: prostate cancer; microRNA; miR-145-5p; epithelial-to-mesenchymal transition; MYO6; biomarker  \n1. Introduction  \nProstate cancer is a heterogeneous disease affecting millions of men worldwide, predominantly in regions with a high human development index [1] . Epithelial-to-mesenchymal transition (EMT) is a key biological process required for the progression and metastasis of prostate cancer [2] . EMT is a dynamic process in which epithelial cells are reprogrammed to exhibit a mesenchymal phenotype. The loss of cell polarity and cell–cell adhesion leads to enhanced migratory capacity, invasiveness, and resistance to apoptosis [3] . Multiple factors regulate the EMT process, including several miRNAs [4] . MicroRNAs (miRNAs) are short, non-coding RNA molecules that play an important role in regulating gene expression atthe post-transcriptional level.  \nmiRNAs bind to specific sequences in the 30 untranslated region (30 UTR) of their target messenger RNAs (mRNAs), resulting in either mRNA degradation or translational repression [5] . By inhibiting the translation of target mRNAs or causing their degradation, miRNAs can effe","cbCaibdoBYRm75qR","https://ap.wps.com/l/cbCaibdoBYRm75qR","pdf",2521286,"English","# Introduction\n## Prostate cancer and EMT as a driver of metastasis\n## MicroRNAs and post-transcriptional gene regulation\n# miR-145-5p rationale and prior evidence\n## Role across multiple cancer types\n# Objectives and study approach","[{\"question\":\"What is the main biological process investigated in this article?\",\"answer\":\"The article focuses on epithelial-to-mesenchymal transition (EMT), which supports prostate cancer progression and metastasis.\"},{\"question\":\"How does miR-145-5p relate to EMT in prostate cancer?\",\"answer\":\"The study tests whether miR-145-5p can suppress EMT in prostate cancer and explores the underlying molecular interaction.\"},{\"question\":\"Which gene is identified as a target of miR-145-5p?\",\"answer\":\"MYO6 is identified and validated as a novel target of miR-145-5p in prostate cancer cells.\"}]","The Suppression of the Epithelial to Mesenchymal Transition in Prostate Cancer - through the Targeting of MYO6 Using MiR-145-5p | PDF",1789996327]