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The study evaluates SAB-BP in a middle cerebral artery occlusion rat model, a ponatinib-induced zebrafish ischemic model, and oxygen-glucose deprivation/reperfusion human umbilical vein endothelial cells. Neuroprotection is assessed by behavioral and histopathology assays, while angiogenesis is measured via zebrafish thrombosis and endothelial functional assays plus Nrf2/HO-1/VEGFA protein analyses. SAB-BP promotes functional vessel formation and up-regulates Nrf2, HO-1, HIF-1α, and VEGFA, with inhibition by an Nrf2 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\n[https://doi.org/10.1080/13880209.2025.2605571](https://doi.org/10.1080/13880209.2025.2605571)  \nReseaRch aRticle  \nThe role of salvianolic acid B and benzoylpaeoniflorin in enhancing angiogenesis through Nrf2/HO-1/VEGFA signaling axis in ischemic stroke recovery  \nchao Zhaoa,b\\#, Xiaodan Baic\\#, Yi Dinga, aiguo Zengb, aidong Wena, Qiang Fub,d and Jingwen Wanga  \naDepartment of Pharmacy, Xijing hospital, Fourth military medical university, Xi’an, china; bDepartment of Pharmaceutical analysis, School of Pharmacy, Xi’an Jiaotong university, Xi’an, china; cDepartment of Pharmacy, tangdu hospital, Fourth military medical university, Xi’an, china; dDepartment of Pharmaceutical analysis, college of Pharmacy, Shenzhen technology university, Shenzhen, china  \nABSTRACT  \nContext: angiogenesis is one of the essential protective mechanisms that promote neural repair and regeneration after ischemic stroke (is). salvianolic acid B (saB) and Benzoyl paeoniflorin (BP) are compounds extracted from the chinese medicines Salvia miltiorrhiza Bunge and Paeonia suffruticosa andrews, respectively.  \nObjective: We investigated whether saB combined with BP alleviated is by promoting micrangium angiogenesis and determined the potential molecular mechanisms. Materials and methods: the impact of saB-BP on angiogenesis after is was investigated in middle cerebral artery occlusion (McaO) rat model, ponatinib-induced ischemic stroke in zebrafish, and human umbilical vein endothelial cells (hUVecs). the neuroprotective effect of saB-BP in rats was assessed using behavior tests and histopathological staining. the cerebral thrombosis assessment and angiogenesis assay were performed in the zebrafish model. cell proliferation and angiogenesis in oxygen-glucose deprivation andreperfusion (OGD/R) hUVecs were assessed through cell viability, tube formation, migration, and invasion assays. Western blot analysis and immunofluorescence staining were used to determine the protein expression levels of Nrf2, hO-1, and VeGFa.  \nResults: the findings indicated that saB-BP significantly reduced neurological impairment following is and promoted the formation of functional vessels in the cerebral ischemic penumbra. Furthermore, saB-BP up-regulated the protein expression of Nrf2, hO-1, hiF-1α, and VeGFa. intriguingly, the pro-angiogenic effect of saB-BP markedly restrained by adding the inhibitor of Nrf2 (Ml385).  \nDiscussion and conclusion: Our study demonstrates that saB-BP enhances angiogenesis following is by modulating the Nrf2/hO-1/VeGFa signaling axis both in vivo and in vitro. saB-BP could serve as a promising therapeutic agent for is recovery.  \nIntroduction  \nARTICLE HISTORY  \nreceived 21 august 2025 revised 8 December 2025 accepted 12 December 2025  \nKEYWORDS  \nSalvianolic acid B; benzoylpaeoniflorin; ischemic stroke; angiogenesis; Nrf2/ho-1/ VegFa signaling axis  \nIschemic stroke (IS), resulting from disrupted cerebral blood flow, causes major neural damage and is a leading global cause of death and disability (Caplan 1991) . The management of IS has traditionally focused on the early reperfusion of at-risk tissue through intravenous thrombolysis and/or endovascular thrombectomy. However, surgical thrombectomy may paradoxically exacerbate ischemia-reperfusion injury, thereby worsening cerebral damage (Krishnan et al. 2021) . Tissue plasminogen activator is the only FDA-approved drug for IS, but its use is limited by a short therapeutic window, reduced effectiveness, and potential for causing hemorrhagic complications. Increased microvessel density in the peri-infarct region indicates that angiogenesis regulation is vital for neural repair and regeneration after IS (Kanazawa et al. 2019; Fang et al. 2023) .  \nCONTACT Jingwen Wang  [wangjingwen8021@163.com](wangjingwen8021@163.com)  Department of Pharmacy, Xijing hospital, Fourth military medical university, No.  \n127 West changle road, Xincheng District, Xi’an ci","cbCaisWD1Agl4Dw9","https://ap.wps.com/l/cbCaisWD1Agl4Dw9","pdf",4223002,"English","## Abstract\n## Introduction\n## Materials and methods\n## Results\n## Discussion and conclusion\n## Keywords","[{\"question\":\"What is the main objective of this study on SAB and BP?\",\"answer\":\"To determine whether salvianolic acid B combined with benzoylpaeoniflorin alleviates ischemic stroke by promoting microvascular angiogenesis and to identify underlying molecular mechanisms.\"},{\"question\":\"Which experimental models are used to test the effects of SAB-BP?\",\"answer\":\"A middle cerebral artery occlusion rat model, a ponatinib-induced ischemic stroke zebrafish model, and oxygen-glucose deprivation/reperfusion-treated human umbilical vein endothelial cells are used.\"},{\"question\":\"How does SAB-BP influence the Nrf2/HO-1/VEGFA signaling axis?\",\"answer\":\"SAB-BP up-regulates Nrf2, HO-1, HIF-1α, and VEGFA protein expression, and its pro-angiogenic effect is reduced by the Nrf2 inhibitor ML385.\"}]","The role of salvianolic acid B and benzoylpaeoniflorin in enhancing angiogenesis through Nrf2/HO-1/VEGFA signaling axis in ischemic stroke recovery | PDF",1790744884]