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CD47 function is analyzed using surgical samples from 96 COAD patients, including immunohistochemistry and multiplex immunofluorescence. Transcriptome integration from TCGA and GEO with R supports links to clinicopathological traits, prognosis, immune infiltration, and microenvironment features. CD47 expression is found to be overexpressed, correlate with advanced TNM stages, and align with immune cell infiltration patterns, with mechanistic support through the CD47/SIRPα pathway and cytokine interaction networks. Results highlight primary-versus-metastatic compositional differences.",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":40,"@type":76,"position":81},"https://docshare.wps.com/document/research-report/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/the-role-of-cd47-in-immune-escape-of-colon-cancer-and-its-correlation-with-heterogeneity-of-tumor-immune-microenvironment/332853/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/the-role-of-cd47-in-immune-escape-of-colon-cancer-and-its-correlation-with-heterogeneity-of-tumor-immune-microenvironment/332853.png","ImageObject",300,407,{"name":92,"@type":93},"Clementine","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-10-24","2026-09-21",true,{"@type":102,"interactionType":103,"userInteractionCount":29},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"What is CD47’s proposed role in colon cancer immune escape?","Question",{"text":112,"@type":113},"CD47 functions as an immune checkpoint by interacting with SIRPα on macrophages, transmitting a “don’t eat me” signal that helps tumor cells evade immune surveillance.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"How was CD47 expression measured and analyzed in this study?",{"text":117,"@type":113},"CD47 was assessed in surgical samples using immunohistochemistry (90 samples) and multiplex immunofluorescence (6 samples). Associations were further studied by integrating TCGA and GEO transcriptome data and using tools for immune infiltration and prognosis.",{"name":119,"@type":110,"acceptedAnswer":120},"What clinical and microenvironment relationships were found for CD47 in COAD?",{"text":121,"@type":113},"Higher CD47 expression was linked to more advanced tumor stages and showed a correlation with immune cell infiltration. CD47 also related to tumor immune microenvironment heterogeneity, including distinct primary versus metastatic compositions and changes involving cytokine/cytokine receptor interactions.","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},332853,1790390316,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":39,"category_name":40,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":29,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":139,"language":140,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":141,"faqs":142,"seo_title":143,"seo_description":67,"update_tm":144,"read_time":145,"preview_page_count":4},1374391974564,"https://ap-avatar.wpscdn.com/avatar/14000253aa45c000a9e?x-image-process=image/resize,m_fixed,w_180,h_180&k=1779874745381141002","Submitted 13 June 2024  \nAccepted 4 November 2024  \nPublished 9 December 2024  \nCorresponding author  \nZhiGang Pei, [17784255157@163.com](17784255157@163.com)[ ](17784255157@163.com)Academic editor  \nBrittany Lasseigne  \nAdditional Information and Declarations can be found on page 21  \nDOI 10.7717/peerj.18579  \n Copyright 2024 Tian et al.  \nDistributed under  \nCreative Commons CC-BY-NC 4.0  \nOPEN ACCESS  \nThe role of CD47 in immune escape of colon cancer and its correlation with heterogeneity of tumor immune microenvironment  \nQiu-Si Tian1 , ChunMei Zhang2 , Zhi-Jun Bao1 and ZhiGang Pei2  \n1 Department of Neurosurgery, 3201 Hospital of Xi'an Jiaotong University Health Science Center, HanZhong, ShaanXi, China  \n2 Department of Pathology, Chongqing University Jiangjin Hospital, ChongQing, China  \nABSTRACT  \nBackground. Cluster of differentiation 47 (CD47), a transmembrane protein, plays a critical role in regulating cellular functions and maintaining immune homeostasis. Its expression has been shown to influence cancer prognosis. In this study, we investigated theroleofCD47 in tumor progression in colon adenocarcinoma (COAD) and evaluated its potential as a target for immunotherapy.  \nMaterials and Methods. We analyzed surgical samples from 96 COAD patients. Immunohistochemical analysis was performed on 90 samples, while the remaining 6 were subjected to multiplex immunofluorescence. To explore the association between CD47 expression and clinicopathological characteristics, we integrated transcriptome data from The Cancer Genome Atlas and the Gene Expression Omnibus using R software. The Tumor Immune Estimation Resource and Kaplan-Meier plotter were utilized to assess the relationship between CD47 expression, patient prognosis, and immune infiltration. Furthermore, the single-cell Tumor Immune System Interaction Database was used to examine the correlation between CD47 expression and the tumor microenvironment (TME). All included patients gave oral and written informed consent. The study was approved by the Ethics Committee of 3201 Hospital (full name: Medical Ethics Committee of 3201 Hospital) .  \nResults. CD47 was found to be overexpressed in various tumors, including COAD. Higher CD47 expression was significantly associated with more advanced tumor stages, including TNM staging, T staging, and N staging (P \u003C 0.05) . A robust correlation was observed between CD47 expression and immune cell infiltration in COAD. Patients with elevated CD47 expression tended to have longer disease-free intervals, although this benefit was diminished in cases with high infiltration of M1 macrophages. The immunosuppressive function of CD47 primarily acted through the CD47/SIRP􀀋 pathway. Additionally, distinct cellular compositions and distributions were identified between primary and metastatic COAD, underscoring the heterogeneity of the TME. CD47 also influenced the TME by modulating cytokine and cytokine receptor interactions.  \nConclusion. CD47 represents a promising prognostic biomarker and a potential target for immunotherapy in COAD. These findings provide new insights into therapeutic strategies aimed at modulating the TME and improving patient outcomes.  \nHow to cite this article Tian Q-S, Zhang CM, Bao Z-J, Pei ZG. 2024. The role ofCD47 in immune escape of colon cancer and its correlation with heterogeneity of tumor immune microenvironment. PeerJ 12:e18579 [http://doi.org/10.7717/peerj.18579](http://doi.org/10.7717/peerj.18579)  \nSubjects Bioinformatics, Cell Biology, Gastroenterology and Hepatology, Oncology Keywords CD47, Immune escape, Tumor microenvironment, COAD, Heterogeneity  \nINTRODUCTION  \nColorectal cancer (CRC) is the fourth most common cancer globally and the third leading cause of cancer-related deaths. Among its subtypes, colon adenocarcinoma (COAD) is the most prevalent, accounting for over 60% of all CRC cases (Bray et al., 2018) . COADis characterized by a complex interplay of genetic, cellular, and environmental factors,","cbCaioi5uP4I1mSM","https://ap.wps.com/l/cbCaioi5uP4I1mSM","pdf",7024991,26,"English","# Abstract\n## Background\n## Materials and Methods\n## Results\n## Conclusion\n# Introduction","[{\"question\":\"What is CD47’s proposed role in colon cancer immune escape?\",\"answer\":\"CD47 functions as an immune checkpoint by interacting with SIRPα on macrophages, transmitting a “don’t eat me” signal that helps tumor cells evade immune surveillance.\"},{\"question\":\"How was CD47 expression measured and analyzed in this study?\",\"answer\":\"CD47 was assessed in surgical samples using immunohistochemistry (90 samples) and multiplex immunofluorescence (6 samples). Associations were further studied by integrating TCGA and GEO transcriptome data and using tools for immune infiltration and prognosis.\"},{\"question\":\"What clinical and microenvironment relationships were found for CD47 in COAD?\",\"answer\":\"Higher CD47 expression was linked to more advanced tumor stages and showed a correlation with immune cell infiltration. CD47 also related to tumor immune microenvironment heterogeneity, including distinct primary versus metastatic compositions and changes involving cytokine/cytokine receptor interactions.\"}]","The role of CD47 in immune escape of colon cancer and its correlation with heterogeneity of tumor immune microenvironment | PDF",1789999769,66]