[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"doc-seo-626810-105":3,"detail-sidebar-cat-0-en-105":81,"doc-detail-626810-en":130},{"code":4,"msg":5,"data":6},0,"ok",{"site_id":7,"language":8,"slug":9,"title":10,"keywords":11,"description":12,"schema_data":13,"social_meta":74,"head_meta":76,"extra_data":78,"updated_unix":80},105,"en","the-relationship-of-gh-and-lep-genes-polymorphism-with-the-qualitative-characteristics-of-sheep-meat","THE RELATIONSHIP OF GH AND LEP GENES POLYMORPHISM WITH THE QUALITATIVE CHARACTERISTICS OF SHEEP MEAT","","DNA sequencing of Soviet Merino sheep was used to identify single nucleotide polymorphisms in the GH and LEP genes related to meat quality. A missense mutation C. 321C>T was found in GH exon 5 and a missense mutation C. 387G>T in LEP exon 3. Allele and genotype frequencies were determined, and associations with muscle tissue qualitative composition were established. Heterozygotes carried more protein (3.3 and 2.4 abs.%) and less moisture (3.3 and 2.4 abs.%) than homozygotes for the wild alleles. Muscle fiber counts were higher in carriers of mutant alleles, while fiber diameter was smaller. “Marbling” scores were higher in heterozygous genotypes, supporting these polymorphisms as genetic markers for evaluating and improving sheep meat quality.",{"@graph":14,"@context":73},[15,34,56],{"@type":16,"itemListElement":17},"BreadcrumbList",[18,23,27,31],{"item":19,"name":20,"@type":21,"position":22},"https://docshare.wps.com","Home","ListItem",1,{"item":24,"name":25,"@type":21,"position":26},"https://docshare.wps.com/document/","Document",2,{"item":28,"name":29,"@type":21,"position":30},"https://docshare.wps.com/document/research-report/","Research & 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polymorphisms in the GH and LEP genes were identified?","Question",{"text":63,"@type":64},"A missense mutation C. 321C>T was identified in the GH gene (exon 5), and a missense mutation C. 387G>T was detected in the LEP gene (exon 3).","Answer",{"name":66,"@type":61,"acceptedAnswer":67},"How do heterozygous genotypes affect protein and moisture in sheep muscle tissue?",{"text":68,"@type":64},"Compared with homozygotes for wild alleles, heterozygous genotypes with mutant alleles C. 321T (GH) and C. 387T (LEP) showed 3.3 and 2.4 abs.% more protein and 3.3 and 2.4 abs.% less moisture.",{"name":70,"@type":61,"acceptedAnswer":71},"What do the results suggest about using these polymorphisms for meat quality improvement?",{"text":72,"@type":64},"The study concludes that polymorphisms C. 321C>T in GH and C. 387G>T in LEP can serve as genetic markers for evaluating and improving the quality of sheep 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GHAND LEP GENES POLYMORPHISM WITH THE QUALITATIVE CHARACTERISTICS  \nOF SHEEP MEAT  \n© 2025 A. V. Skokova*, L. N. Skorykh, А. V. Sukhoveeva, E. Yu. Safaryan, and N. I. Efimovaa  \nNorth Caucasus Federal Agrarian Research Centre, Mikhailovsk, Russia  \n*е-[mail: antoninaskokova@mail.ru](mail: antoninaskokova@mail.ru)  \nReceived July 18, 2024  \nRevised September 14, 2024  \nAccepted September 18, 2024  \nAbstract. DNA sequencing of Soviet Merino sheep was performed to identify single nucleotide polymorphisms (ONP) in the GHand LEP genes associated with meat quality indicators. Based on the analysis, a missense mutation C. 321C>T was found in the structure of the GH gene in exon 5, leading to the replacement of arginine with glycine; in the LEP gene in exon 3, a missense mutation C. 387G>Twas detected, which results in the replacement of the amino acid valine with leucine. The frequency of occurrence of alleles and genotypes in the detected single polymorphisms has been established. 321C>Tof the GH gene and C. 387G>T ofthe LEP gene. The relationship between the qualitative composition of muscle tissue in sheep and their genotypic variants for both studied genes has been revealed. The muscle tissue of the heterozygous genotype according to the mutant alleles C. 321T ofthe GH gene and C. 387>Tofthe LEP gene contained more protein by 3.3 and 2.4 abs.%, but less moisture by 3.3 and 2.4 abs.% than in sheep of the homozygous genotype according to the wild alleles C. 321C of the GH gene and C. 387Gofthe LEP gene. Studies of the longest back muscle of Soviet Merino sheep revealed a greater number of muscle fibers in carriers of mutant alleles C. 321T ofthe GH gene and C. 387>T ofthe LEP gene by  \n4.7 and 7.6%, but a smaller diameter of the muscle fiber by 8.9 and 5.0%, compared with muscle cells of sheep homozygous for wild alleles C. 321C of the GH gene and C. 387G ofthe LEP gene. The overall assessment of the \"marbling\" of muscle tissue in sheep of heterozygous genotypes for the mutant alleles C. 321T ofthe GH gene and C. 387>T ofthe LEP gene is 2.3 and 1.5 points higher than in homozygotes for the reference alleles C. 321C of the GH gene and C. 387G ofthe LEP gene. The results obtained allow us to consider the polymorphisms C. 321C>T in the GH gene and C. 387G>T in the LEP gene as genetic markers for evaluating and improving the quality of sheep meat.  \nKeywords: sheep, DNA, PCR, genotyping, sequencing, polymorphism, single nucleotide substitutions, productivity  \nDOI: 10. 31857/S00166758250207e9  \nINTRODUCTION  \nThe economic efficiency of sheep breeding enterprises generally depends on the productive and reproductive indicators of sheep. Currently, the most important tasks facing sheep breeding are increasing the quantity and quality of meat productivity, reducing the cost of consumed feed per unit of production, genetic monitoring, controlling the selection process of breeding animals, and finding new applicable strategies that increase the economic profit of the industry [1, 2] . Selection  \nusing molecular biology and biotechnology approaches allows for more effective and accurate selection results. Therefore, the identification of genes that influence sheep productivity serves as the first and most important stage in selection using genetic markers [3] .  \nMarker-assisted selection is a new approach for animal breeding programs that are based on marker information in addition to any available traditional information, as well as in the case of late traits that manifest during the animal's life or are regulated by several genes [4] . The use of molecular markers provides a method for  \n68 SKOKOVA et al.  \nselecting animals for a wide range of traits at any stage of life and increases the predictability of an individual's mature phenotype. At the same time, determining gene polymorphism is vital in animal breeding to ident","cbCail6RRVtKI5L0","https://ap.wps.com/l/cbCail6RRVtKI5L0","pdf",201801,"English","# Introduction\n## Economic efficiency and marker-assisted selection\n## Growth hormone (GH) gene background\n## Leptin (LEP) gene background\n# Abstract\n## Identified polymorphisms and genotypic frequencies\n## Associations with muscle tissue composition\n## Associations with muscle fibers and marbling","[{\"question\":\"Which polymorphisms in the GH and LEP genes were identified?\",\"answer\":\"A missense mutation C. 321C\\u003eT was identified in the GH gene (exon 5), and a missense mutation C. 387G\\u003eT was detected in the LEP gene (exon 3).\"},{\"question\":\"How do heterozygous genotypes affect protein and moisture in sheep muscle tissue?\",\"answer\":\"Compared with homozygotes for wild alleles, heterozygous genotypes with mutant alleles C. 321T (GH) and C. 387T (LEP) showed 3.3 and 2.4 abs.% more protein and 3.3 and 2.4 abs.% less moisture.\"},{\"question\":\"What do the results suggest about using these polymorphisms for meat quality improvement?\",\"answer\":\"The study concludes that polymorphisms C. 321C\\u003eT in GH and C. 387G\\u003eT in LEP can serve as genetic markers for evaluating and improving the quality of sheep meat.\"}]","THE RELATIONSHIP OF GH AND LEP GENES POLYMORPHISM WITH THE QUALITATIVE CHARACTERISTICS OF SHEEP MEAT | PDF",1792540741]