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Prior work suggested impaired one-week OTM in NLRP3-deficient mice, but its role in long-term OTM is unclear. Materials and Methods: OTM was evaluated after 7, 14, and 21 days of force in NLRP3−/− and wild-type littermates using micro-CT for tooth movement and bone quality, and TRAP staining to quantify osteoclast activity. Results: NLRP3 loss delayed OTM at 2 weeks, with catch-up by day 21, while alveolar bone loss occurred regardless of NLRP3 expression. Conclusion: NLRP3 is critical early but not later during OTM.",{"@graph":69,"@context":123},[70,84,106],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":35,"@type":76,"position":81},"https://docshare.wps.com/document/healthcare/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/the-nlrp3-inflammasome-regulates-orthodontic-tooth-movement-research-article/452779/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/the-nlrp3-inflammasome-regulates-orthodontic-tooth-movement-research-article/452779.png","ImageObject",300,407,{"name":92,"@type":93},"dawugda","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-11-06","2026-09-30",true,{"@type":102,"interactionType":103,"userInteractionCount":105},"InteractionCounter",{"@type":104},"ViewAction",11,{"@type":107,"mainEntity":108},"FAQPage",[109,115,119],{"name":110,"@type":111,"acceptedAnswer":112},"What was the study’s main objective regarding NLRP3 in orthodontic tooth movement?","Question",{"text":113,"@type":114},"To determine whether the NLRP3 inflammasome influences long-term orthodontic tooth movement beyond the previously reported effects at one week.","Answer",{"name":116,"@type":111,"acceptedAnswer":117},"How was orthodontic tooth movement assessed in this research?",{"text":118,"@type":114},"After 7, 14, and 21 days of force application, micro-computed tomography (μCT) measured molar movement and bone quality.",{"name":120,"@type":111,"acceptedAnswer":121},"What did the results show about the timing of NLRP3’s effect on OTM?",{"text":122,"@type":114},"OTM was delayed in NLRP3−/− mice at 2 weeks compared with wild-type, but movement regained speed and matched controls by day 21.","https://schema.org",{"og:url":83,"og:type":125,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":127,"canonical":83},"index,follow",{"doc_id":129,"site_id":62},452779,1790980579,{"code":4,"msg":5,"data":132},{"doc_id":129,"user_id":133,"nickname":92,"user_avatar":134,"doc_module":4,"category_id":34,"category_name":35,"doc_title":65,"doc_description":67,"doc_content":135,"file_id":136,"file_url":137,"file_type":138,"file_size":139,"view_count":105,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":52,"language":140,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":141,"faqs":142,"seo_title":143,"seo_description":67,"update_tm":144,"read_time":145,"preview_page_count":4},3985747870947,"https://ap-avatar.wpscdn.com/davatar_3d24733baf745e90a7e4bdd5f77d97b2","Orthodontics & Craniofacial Research  \nRESEARCH ARTICLE  OPEN ACCESS   \nThe NLRP3 Inflammasome Regulates Orthodontic Tooth Movement  \nLisa Latzko1  | Gerald Degenhart2 | Michael J. Blumer3 | Reinhard Gruber4  | Johannes Haybäck5,6 | Claudia Manzl7 | Adriano G. Crismani1  \n1University Hospital for Orthodontics, Department of Dental and Oral Medicine and Cranio-Maxillofacial and Oral Surgery, Medical University of Innsbruck, Innsbruck, Austria | 2Department of Radiology, Core Facility MicroCT, Medical University Innsbruck, Innsbruck, Austria | 3Institute of Clinical and Functional Anatomy, Medical University of Innsbruck, Innsbruck, Austria | 4Department of Oral Biology, Medical University of Vienna, Vienna, Austria | 5Tyrolpath Obrist Brunhuber GmbH, Zams, Austria | 6Diagnostic and Research Institute of Pathology, Diagnostic and Research Center of Molecular BioMedicine, Medical University of Graz, Graz, Austria | 7Institute of Pathology, Neuropathology and Molecular Pathology, Medical University Innsbruck, Innsbruck, Austria  \nCorrespondence: Lisa Latzko ([lisa.latzko@i-med.ac.at](lisa.latzko@i-med.ac.at))  \nReceived: 30 July 2024 | Revised: 19 September 2025 | Accepted: 5 November 2025  \nFunding: This work was supported by the grant from the Tyrolean Science Fund of the State of Tyrol. (Grand No. F.45069/8-2022) .  \nKeywords: micro-CT | NLRP3 Inflammasome | orthodontic tooth movement | TRAP  \nABSTRACT  \nObjective: The NLRP3 inflammasome is a damage sensor driving pyroptotic cell death during orthodontic tooth movement (OTM). Recent studies have revealed impaired one-week OTM in mice lacking NLRP3. However, the relevance of NLRP3 during long-term OTM remains unknown.  \nMaterials and Methods: OTM was analysed after 7, 14 and 21 days of force application in mice lacking NLRP3 (NLRP3−/−), wild-type (WT) male and female littermates. Micro-computed tomography (μCT) was used to assess molar movement and bone quality. Tartrate-resistant acid phosphatase (TRAP) staining was used to visualise osteoclastic activity.  \nResults: We show here that after 2 weeks of force application, OTM was delayed in mice lacking NLRP3 compared to WT mice. Interestingly, OTM regained speed in NLRP3−/− mice catching up with the WT controls at day 21. Independent of NLRP3 expression, OTM caused alveolar bone loss compared to the untreated contralateral side. NLRP3−/ − mice showed a diminished number of TRAP-positive osteoclasts than WT male mice 14 and 21 days after OTM induction.  \nConclusion: The results of this study suggest that NLRP3 is relevant during the early but not later stages of OTM.  \n1 | Introduction  \nIn mammals, the process of orthodontic tooth movement (OTM) is primarily based on the initiation of an inflammatory response within the periodontal ligament (PDL) and alveolar bone, triggered by localised hypoxia [1]. The initial events involve cellular alterations in the PDL, followed by an upregulation ofinflammatory mediators including prostaglandins (PGs), interleukins (ILs such as IL-1ß, -6 and-18), members of the tumour necrosis factor (TNF)-α superfamily, and the receptor activator of nuclear factor  \nligand (RANKL)/osteoprotegerin axis within the periodontal tissues [2]. In the first phase of OTM (up to 24 h) [3], the tooth is displaced within its alveolar socket, whereas in the second phase (2–10 weeks), acute inflammation turns into a chronic state, leading to osteoclastic bone resorption in compressed areas andosteoblastic bone formation in areas of tension [4]. This remodelling process is guided by signalling factors that establish local gradients to regulate bone and PDL remodelling for effective tooth displacement [5]. Especially IL-1β is known to be one of the most potent cytokines in the periodontal environment during the  \nThis is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited,","cbCaiaSJw8wnjup5","https://ap.wps.com/l/cbCaiaSJw8wnjup5","pdf",1245168,"English","# Abstract\n# Objective\n# Materials and Methods\n# Results\n# Conclusion\n# Introduction","[{\"question\":\"What was the study’s main objective regarding NLRP3 in orthodontic tooth movement?\",\"answer\":\"To determine whether the NLRP3 inflammasome influences long-term orthodontic tooth movement beyond the previously reported effects at one week.\"},{\"question\":\"How was orthodontic tooth movement assessed in this research?\",\"answer\":\"After 7, 14, and 21 days of force application, micro-computed tomography (μCT) measured molar movement and bone quality.\"},{\"question\":\"What did the results show about the timing of NLRP3’s effect on OTM?\",\"answer\":\"OTM was delayed in NLRP3−/− mice at 2 weeks compared with wild-type, but movement regained speed and matched controls by day 21.\"}]","The NLRP3 Inflammasome Regulates Orthodontic Tooth Movement - research article | PDF",1790737683,25]