[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"detail-sidebar-cat-0-en-105":3,"doc-seo-354295-105":59,"doc-detail-354295-en":131},{"code":4,"msg":5,"data":6},0,"success",[7,13,18,23,28,33,38,43,48,51,55],{"id":8,"doc_module":4,"doc_module_name":9,"category_name":10,"show_sort_weight":11,"slug":12},1,"Document","Story & Novel",90,"story-novel",{"id":14,"doc_module":4,"doc_module_name":9,"category_name":15,"show_sort_weight":16,"slug":17},2,"Literature",80,"literature",{"id":19,"doc_module":4,"doc_module_name":9,"category_name":20,"show_sort_weight":21,"slug":22},4,"Exam",70,"exam",{"id":24,"doc_module":4,"doc_module_name":9,"category_name":25,"show_sort_weight":26,"slug":27},5,"Comic",60,"comic",{"id":29,"doc_module":4,"doc_module_name":9,"category_name":30,"show_sort_weight":31,"slug":32},6,"Technology",50,"technology",{"id":34,"doc_module":4,"doc_module_name":9,"category_name":35,"show_sort_weight":36,"slug":37},7,"Healthcare",40,"healthcare",{"id":39,"doc_module":4,"doc_module_name":9,"category_name":40,"show_sort_weight":41,"slug":42},8,"Research & Report",30,"research-report",{"id":44,"doc_module":4,"doc_module_name":9,"category_name":45,"show_sort_weight":46,"slug":47},9,"Religion & Spirituality",20,"religion-spirituality",{"id":46,"doc_module":4,"doc_module_name":9,"category_name":49,"show_sort_weight":46,"slug":50},"World Cup","world-cup",{"id":52,"doc_module":4,"doc_module_name":9,"category_name":53,"show_sort_weight":52,"slug":54},10,"Lifestyle","lifestyle",{"id":56,"doc_module":4,"doc_module_name":9,"category_name":57,"show_sort_weight":24,"slug":58},19,"General","general",{"code":4,"msg":60,"data":61},"ok",{"site_id":62,"language":63,"slug":64,"title":65,"keywords":66,"description":67,"schema_data":68,"social_meta":124,"head_meta":126,"extra_data":128,"updated_unix":130},105,"en","the-mechanism-of-fibronectin-1-promoting-papillary-thyroid-cancer-progression-by-regulating-anoikis-resistance","The mechanism of fibronectin 1 promoting papillary thyroid cancer progression by regulating anoikis resistance","","Fibronectin 1 (FN1) is overexpressed in multiple cancers and is linked to tumor progression, yet its role in papillary thyroid cancer (PTC) anoikis resistance remains unclear. This study combines TCGA- and GeneCards-based bioinformatics with experimental validation in PTC tissues and cell lines to identify FN1 as an anoikis-related hub gene. FN1 knockdown reduces PTC proliferation, migration, invasion and increases anoikis, while FN1 overexpression reverses these effects. Mechanistically, FN1 modulates apoptosis via a BCL2L1/BAD axis.",{"@graph":69,"@context":123},[70,84,106],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":35,"@type":76,"position":81},"https://docshare.wps.com/document/healthcare/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/the-mechanism-of-fibronectin-1-promoting-papillary-thyroid-cancer-progression-by-regulating-anoikis-resistance/354295/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/the-mechanism-of-fibronectin-1-promoting-papillary-thyroid-cancer-progression-by-regulating-anoikis-resistance/354295.png","ImageObject",300,407,{"name":92,"@type":93},"Grenda","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-10-30","2026-09-22",true,{"@type":102,"interactionType":103,"userInteractionCount":105},"InteractionCounter",{"@type":104},"ViewAction",11,{"@type":107,"mainEntity":108},"FAQPage",[109,115,119],{"name":110,"@type":111,"acceptedAnswer":112},"What does the study investigate about FN1 in papillary thyroid cancer?","Question",{"text":113,"@type":114},"The study investigates how Fibronectin 1 (FN1) regulates anoikis resistance and promotes papillary thyroid cancer progression.","Answer",{"name":116,"@type":111,"acceptedAnswer":117},"How were FN1’s effects on anoikis, proliferation, migration, and invasion tested?",{"text":118,"@type":114},"FN1 expression and anoikis were measured in PTC tissues, adjacent normal tissues, and cell lines using IHC, TUNEL, qRT-PCR, and Western blot. Functional assays with knockdown or overexpression assessed proliferation (CCK-8), migration, and invasion (Transwell).",{"name":120,"@type":111,"acceptedAnswer":121},"What mechanism links FN1 to anoikis resistance in PTC?",{"text":122,"@type":114},"FN1 enhances anoikis resistance by up-regulating anti-apoptotic proteins BCL2L1 and Caspase-3 and down-regulating pro-apoptotic protein BAD, supporting tumorigenic behavior through an implicated BCL2L1/BAD axis.","https://schema.org",{"og:url":83,"og:type":125,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":127,"canonical":83},"index,follow",{"doc_id":129,"site_id":62},354295,1790124046,{"code":4,"msg":5,"data":132},{"doc_id":129,"user_id":133,"nickname":92,"user_avatar":134,"doc_module":4,"category_id":34,"category_name":35,"doc_title":65,"doc_description":67,"doc_content":135,"file_id":136,"file_url":137,"file_type":138,"file_size":139,"view_count":105,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":140,"language":141,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":142,"faqs":143,"seo_title":144,"seo_description":67,"update_tm":145,"read_time":146},7971474921005,"https://ap-avatar.wpscdn.com/davatar_29158cc5080c5b710cf443261637dec0","[www. nature.com/scientificreports](www. nature.com/scientificreports)  \nOPEN  \nThe mechanism offibronectin 1 promoting papillary thyroid cancer progression by regulating anoikis resistance  \nShengcan Zhang1,2, Xue Gu1,2, Xinyu Zhai1,2, Hanzhao Huang1,2, Renjie Zhang1,2 & Hui Ye1,2􀀍  \nBackground: Papillary thyroid cancer is a common head and neck malignancy with a globally rising incidence. While most patients attain favorable prognosis following standard treatment, a subset develops recurrence and metastasis—two key factors that compromise clinical outcomes. Anoikis resistance is a prerequisite for tumor metastasis; however, the role and mechanism of Fibronectin 1 (FN1)—a molecule overexpressed in multiple cancers and linked to tumor progression—in papillary thyroid cancer anoikis resistance remain undefined. Objective: This study therefore investigated the function and regulatory mechanism of FN1 in papillary thyroid cancer anoikis resistance using a combination of bioinformatics analyses and experimental validation. Methods: First, using The Cancer Genome Atlas (TCGA) dataset and anoikis-related genes from GeneCards, we identified FN1 asan anoikis-related hub gene in thyroid cancer through bioinformatics analyses, including consensus clustering, weighted gene co-expression network analysis (WGCNA), and protein-protein interaction (PPI) network analysis. Its clinical relevance was further analyzed from the perspectives of pathological typing, survival prognosis, immune cell infiltration (via xCell) and single-cell expression patterns.  \nNext, we performed validation using papillary thyroid cancer tissues, adjacent normal tissues, and cell lines (8305c, BCPAP, Nthy-ori 3 − 1): FN1 expression and cancer cell anoikis were detected by immunohistochemistry (IHC) and TUNEL staining; FN1 mRNA and protein levels were quantified via qRT-PCR and Western blot. Finally, we modulated FN1 expression through RNA interference (for knockdown) . Cell proliferation, migration, and invasion were assessed using CCK-8 and Transwell assays, while Western blot was applied to measure the expression of apoptosis-related proteins. Results: FN1 is highly expressed in papillary thyroid cancer tissues and cell lines and is associated with poor tumor prognosis. Silencing FN1 can inhibit the proliferation, migration and invasion of papillary thyroid cancer cells and increase the anoikis rate. Overexpression of FN1 showed the opposite effect. Mechanistically, FN1 can up-regulate anti-apoptotic proteins BCL2L1 and Caspase-3, and down-regulate pro-apoptotic protein BAD, thereby enhancing anoikis resistance. Conclusions: FN1 is highly expressed in papillary thyroid cancer and correlates with poor prognosis. Mechanistically, FN1 enhances papillary thyroid cancer cell tumorigenic potential by regulating proliferation, migration, invasion and apoptotic progression via the BCL2L1/BAD axis, thus driving tumor development. FN1 may act as a promising therapeutic target for advanced papillary thyroid cancer.  \nKeywords Fibronectin 1, Papillary thyroid cancer, Anoikis resistance, Tumor progression  \nAbbreviations  \nAJCC American joint committee on cancer  \nAKT Protein kinase B  \nAOD Average optical density ARGs Anoikis-related genes  \nBP Biological processes  \nCC Cellular components  \nCCK-8 Cell counting kit-8  \n1Clinical Medical College, Guizhou Medical University, Guiyang 551113, China. 2Thyroid Surgery Department, The Affiliated Hospital of Guizhou Medical University, Guiyang 550004, China. 􀀍 email: [1442619877@qq.com](1442619877@qq.com)  \n[www. nature.com/scientificreports/](www. nature.com/scientificreports/)  \nDE-ARGs DEGs  \nDFS  \nDMEM  \nECM  \nEDB  \nERK  \nFAK  \nFBS  \nFDR  \nFN1  \nFPKM  \nFTC  \nGEO  \nGO  \nHR  \nIHCITGB1 KEGG  \nMAPK  \nMF  \nMTC  \nNC  \nPI3K PLA2R1 PPI  \nPTCqRT-PCRscRNA-seq  \nsiRNATCGATIMPs TPM  \ntSNE  \nVEGF WGCNA  \nDifferentially expressed anoikis-related genes  \nDifferentially expressed genes Disease-free survival  \nDulbecco’s modified eagle medium Ext","cbCaib7h4ErFQ4iD","https://ap.wps.com/l/cbCaib7h4ErFQ4iD","pdf",3487377,18,"English","# Background\n# Objective\n# Methods\n## Bioinformatics analyses\n## Experimental validation\n# Results\n# Conclusions","[{\"question\":\"What does the study investigate about FN1 in papillary thyroid cancer?\",\"answer\":\"The study investigates how Fibronectin 1 (FN1) regulates anoikis resistance and promotes papillary thyroid cancer progression.\"},{\"question\":\"How were FN1’s effects on anoikis, proliferation, migration, and invasion tested?\",\"answer\":\"FN1 expression and anoikis were measured in PTC tissues, adjacent normal tissues, and cell lines using IHC, TUNEL, qRT-PCR, and Western blot. Functional assays with knockdown or overexpression assessed proliferation (CCK-8), migration, and invasion (Transwell).\"},{\"question\":\"What mechanism links FN1 to anoikis resistance in PTC?\",\"answer\":\"FN1 enhances anoikis resistance by up-regulating anti-apoptotic proteins BCL2L1 and Caspase-3 and down-regulating pro-apoptotic protein BAD, supporting tumorigenic behavior through an implicated BCL2L1/BAD axis.\"}]","The mechanism of fibronectin 1 promoting papillary thyroid cancer progression by regulating anoikis resistance | PDF",1790109920,45]