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This study evaluated cisplatin effects on MSC defining features, differentiation, and migration in vitro. MSCs from four human donors were cultured with or without cisplatin for 24 h at toxic and subcytotoxic levels. Surface markers were assessed by flow cytometry, migration by scratch assay, and differentiation by histology and RT-qPCR.",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":35,"@type":76,"position":81},"https://docshare.wps.com/document/healthcare/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/the-influence-of-cisplatin-on-functionality-and-surface-characteristics-of-mesenchymal-stromal-cells-in-vitro/462425/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/the-influence-of-cisplatin-on-functionality-and-surface-characteristics-of-mesenchymal-stromal-cells-in-vitro/462425.png","ImageObject",300,407,{"name":92,"@type":93},"Sarah ","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-11-09","2026-09-30",true,{"@type":102,"interactionType":103,"userInteractionCount":52},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"What was the main aim of the study on cisplatin and MSCs?","Question",{"text":112,"@type":113},"To evaluate how cisplatin influences MSC defining characteristics, including differentiation ability and migration potential.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"Which methods were used to assess MSC surface characteristics, differentiation, and migration?",{"text":117,"@type":113},"Flow cytometry was used for surface phenotype markers, scratch assay for migration, and histology plus RT-qPCR for differentiation-related markers.",{"name":119,"@type":110,"acceptedAnswer":120},"How did cisplatin exposure affect MSCs in terms of migration and differentiation?",{"text":121,"@type":113},"Cell migration was not restricted under the conditions used. mRNA changes suggested reduced leptin (adipogenic marker) and increased SOX9 (chondrogenic marker), while histology showed no difference for adipogenic, chondrogenic, or osteogenic differentiation up to 10 µM cisplatin.","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},462425,1790814096,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":34,"category_name":35,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":52,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":56,"language":139,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":140,"faqs":141,"seo_title":142,"seo_description":67,"update_tm":143,"read_time":144,"preview_page_count":4},962085320529,"https://ap-avatar.wpscdn.com/davatar_9964176cb1d06d4a9deccf72a44ae3dc","Article  \nThe Influence of Cisplatin on Functionality and Surface Characteristics of Mesenchymal Stromal Cells In Vitro  \nArmin von Fournier 1, *, Totta Ehret Kasemo 1, Miguel Goncalves 1, Stephan Hackenberg 1, Marietta Herrmann 2,3, Marianne Schmidt 1, Manuel Stöth 1, Till Meyer 1, Thomas Gehrke 1 and Agmal Scherzad 1  \nAcademic Editors: Manuel Aureliano, Juan Llopis and Agnieszka Scibior  \nReceived: 4 October 2025  \nRevised: 12 December 2025  \nAccepted: 14 December 2025  \nPublished: 21 December 2025  \nCopyright: © 2025 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license.  \n1 Department of Oto-Rhino-Laryngology, Head and Neck Surgery, University Hospital Wuerzburg,  \n97080 Wuerzburg, Germany  \n2 IZKF Group Tissue Regeneration in Musculoskeletal Diseases, University Hospital Wuerzburg,  \n97074 Wuerzburg, Germany  \n3 Bernhard-Heine-Centrum for Locomotion Research, Julius-Maximilians-Universitat Wuerzburg,  \n97074 Wuerzburg, Germany  \n* Correspondence: fournier_[a@ukw.de](a@ukw.de)  \nAbstract  \nMesenchymal stromal cells (MSCs) are multipotent and play an important role in regenerative processes such as wound healing. Data on possible changes and functional restrictions of MSCs due to cisplatin chemotherapy, for example, in the treatment of head and neck cancer, diverge. The aim of this study was to evaluate the influence of cisplatin on MSCs with regard to their defining characteristics and their ability to differentiate and to migrate. MSCs from four human donors (a 59-year-old man, a 63-year-old woman, a 70-year-old man, anda 61-year-old man) were cultured in vitro with and without cisplatin for 24 h, and toxic and subcytotoxic concentrations were determined using an MTT. We then examined the surface phenotype markers (flow cytometry), migration (scratch assay), histological differentiation markers (adipo-, chondro-, osteogenic), and the expression of selected line-associated genes in real-time quantitative PCR (RT-qPCR) (LEP, SOX9, RUNX2) . These characteristics were evaluated after treatment with different subcytotoxic, clinically relevant doses ofcisplatin. Flow cytometry confirmed the presence of MSCs-characteristic surface markers, which remained stable under treatment with subcytotoxic doses of cisplatin. Cisplatin exposure reduced the mRNA abundance of leptin (a marker for adipogenic differentiation) but increased SOX9 mRNA abundance (chondrogenic differentiation) . RUNX (osteogenic differentiation) did not change post cisplatin exposure. Histological analysis showed no difference with regard to osteogenic, chondrogenic, and adipogenic differentiation at doses up to 10 µM cisplatin. Cell migration was not restricted by cisplatin exposure under the conditions used here. The characteristics of MSCs were not different to controls post cisplatin exposure. mRNA analysis suggested induced changes by cisplatin, although this effect was not histologically detectable even at high doses. Based on the single-molecule markers used here, indications for an inhibitory effect of cisplatin on adipogenic differentiation and a rather enhancing effect on chondrogenic and osteogenic differentiation maybe hypothesized. The process observed here could further aggravate the already serious problem of malnutrition in head and neck cancer patients, for example. Taken together though, our study confirms overall MSCs tolerance towards cisplatin.  \nKeywords: cisplatin; MSCs; mesenchymal stromal cells; stem cell characteristics; differentiation; migration; functionality  \n1. Introduction  \nMesenchymal stem cells (MSCs) are multipotent cells with features which are promising in, e.g., tissue engineering and other medical research, but may also play so far poorly understood roles in manipulating, for instance, tumor microenvironments. Cisplatin is a commonly used chemotherapeutic compound. Understanding potential intera","cbCaic87mSVjFd8b","https://ap.wps.com/l/cbCaic87mSVjFd8b","pdf",4270361,"English","# Abstract\n# 1. Introduction","[{\"question\":\"What was the main aim of the study on cisplatin and MSCs?\",\"answer\":\"To evaluate how cisplatin influences MSC defining characteristics, including differentiation ability and migration potential.\"},{\"question\":\"Which methods were used to assess MSC surface characteristics, differentiation, and migration?\",\"answer\":\"Flow cytometry was used for surface phenotype markers, scratch assay for migration, and histology plus RT-qPCR for differentiation-related markers.\"},{\"question\":\"How did cisplatin exposure affect MSCs in terms of migration and differentiation?\",\"answer\":\"Cell migration was not restricted under the conditions used. mRNA changes suggested reduced leptin (adipogenic marker) and increased SOX9 (chondrogenic marker), while histology showed no difference for adipogenic, chondrogenic, or osteogenic differentiation up to 10 µM cisplatin.\"}]","The Influence of Cisplatin on Functionality and Surface Characteristics of Mesenchymal Stromal Cells In Vitro | PDF",1790764048,48]