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ATPase activity was quantified spectrophotometrically in patient samples (n=10) and mechanistic calcium dynamics were examined in mouse hepatocytes. Rat liver mitochondria were analyzed for respiration and oxidative phosphorylation. TLC–S increased Na+/K+ ATPase in cancer, decreased Mg2+ ATPase in healthy mucosa, altered rat liver Ca2+ and Na+/K+ ATPases, and induced cytosolic Ca2+ signals attenuated by NED-19.",{"@graph":14,"@context":73},[15,34,56],{"@type":16,"itemListElement":17},"BreadcrumbList",[18,23,27,31],{"item":19,"name":20,"@type":21,"position":22},"https://docshare.wps.com","Home","ListItem",1,{"item":24,"name":25,"@type":21,"position":26},"https://docshare.wps.com/document/","Document",2,{"item":28,"name":29,"@type":21,"position":30},"https://docshare.wps.com/document/healthcare/","Healthcare",3,{"item":32,"name":10,"@type":21,"position":33},"https://docshare.wps.com/document/the-impact-of-3-sulfo-taurolithocholic-acid-on-atpase-activity-in-patients-colorectal-cancer-and-normal-colon-tissues-and-its-hepatic-effects-in-rodents/340654/",4,{"url":32,"name":10,"@type":35,"image":36,"author":41,"headline":10,"publisher":44,"fileFormat":47,"inLanguage":8,"description":12,"dateModified":48,"datePublished":49,"encodingFormat":47,"isAccessibleForFree":50,"interactionStatistic":51},"DigitalDocument",{"url":37,"@type":38,"width":39,"height":40},"https://docshare.wps.com/thumbnails/the-impact-of-3-sulfo-taurolithocholic-acid-on-atpase-activity-in-patients-colorectal-cancer-and-normal-colon-tissues-and-its-hepatic-effects-in-rodents/340654.png","ImageObject",300,407,{"name":42,"@type":43},"Genevieve","Person",{"url":19,"name":45,"@type":46},"DocShare","Organization","application/pdf","2026-10-29","2026-09-22",true,{"@type":52,"interactionType":53,"userInteractionCount":55},"InteractionCounter",{"@type":54},"ViewAction",7,{"@type":57,"mainEntity":58},"FAQPage",[59,65,69],{"name":60,"@type":61,"acceptedAnswer":62},"What was the main hypothesis of the study?","Question",{"text":63,"@type":64},"The study hypothesized that bile acids can contribute to tumorigenesis by disrupting ion transport and ATPase activity in the intestinal mucosa.","Answer",{"name":66,"@type":61,"acceptedAnswer":67},"How did TLC–S affect Na+/K+ ATPase activity in colorectal cancer samples?",{"text":68,"@type":64},"TLC–S increased Na+/K+ ATPase activity by about 1.5 times in colorectal cancer samples compared with controls (p ≤ 0.05).",{"name":70,"@type":61,"acceptedAnswer":71},"What hepatic and calcium-related effects were observed in rodents?",{"text":72,"@type":64},"In rat liver, TLC–S decreased Ca2+ ATPase and Na+/K+ ATPase activities while increasing basal Mg2+ ATPase activity (p ≤ 0.05). In mouse hepatocytes, TLC–S induced cytosolic Ca2+ signals that were partially attenuated by NED-19.","https://schema.org",{"og:url":32,"og:type":75,"og:title":10,"og:site_name":45,"og:description":12},"article",{"robots":77,"canonical":32},"index,follow",{"doc_id":79,"site_id":7},340654,1790103888,{"code":4,"msg":82,"data":83},"success",[84,88,92,96,101,106,109,114,119,122,126],{"id":22,"doc_module":4,"doc_module_name":25,"category_name":85,"show_sort_weight":86,"slug":87},"Story & Novel",90,"story-novel",{"id":26,"doc_module":4,"doc_module_name":25,"category_name":89,"show_sort_weight":90,"slug":91},"Literature",80,"literature",{"id":33,"doc_module":4,"doc_module_name":25,"category_name":93,"show_sort_weight":94,"slug":95},"Exam",70,"exam",{"id":97,"doc_module":4,"doc_module_name":25,"category_name":98,"show_sort_weight":99,"slug":100},5,"Comic",60,"comic",{"id":102,"doc_module":4,"doc_module_name":25,"category_name":103,"show_sort_weight":104,"slug":105},6,"Technology",50,"technology",{"id":55,"doc_module":4,"doc_module_name":25,"category_name":29,"show_sort_weight":107,"slug":108},40,"healthcare",{"id":110,"doc_module":4,"doc_module_name":25,"category_name":111,"show_sort_weight":112,"slug":113},8,"Research & Report",30,"research-report",{"id":115,"doc_module":4,"doc_module_name":25,"category_name":116,"show_sort_weight":117,"slug":118},9,"Religion & Spirituality",20,"religion-spirituality",{"id":117,"doc_module":4,"doc_module_name":25,"category_name":120,"show_sort_weight":117,"slug":121},"World Cup","world-cup",{"id":123,"doc_module":4,"doc_module_name":25,"category_name":124,"show_sort_weight":123,"slug":125},10,"Lifestyle","lifestyle",{"id":127,"doc_module":4,"doc_module_name":25,"category_name":128,"show_sort_weight":97,"slug":129},19,"General","general",{"code":4,"msg":82,"data":131},{"doc_id":79,"user_id":132,"nickname":42,"user_avatar":133,"doc_module":4,"category_id":55,"category_name":29,"doc_title":10,"doc_description":12,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":55,"is_deleted":4,"is_public":22,"is_downloadable":22,"audit_status":22,"page_count":139,"language":140,"language_code":8,"site_id":7,"html_lang":8,"table_of_contents":141,"faqs":142,"seo_title":143,"seo_description":12,"update_tm":144,"read_time":112},1374391974585,"https://ap-avatar.wpscdn.com/davatar_276721f389ce27ea32af1340a28f341c","TYPE Original Research PUBLISHED 05 December 2024 DOI 10.3389/fvets.2024.1480122  \nOPEN ACCESS  \nEDITED BY  \nXiao-Yu Liu,  \nSouthern University of Science and Technology, China  \nREVIEWED BY  \nMahamane Talphi Diakite,  \nUniversité des Sciences, des Techniqueset des Technologies de Bamako, Mali Wang Jian,  \nWuxi Second Geriatric Hospital, China  \n*CORRESPONDENCE  \nIvan Kushkevych  \n [kushkevych@mail.muni.cz](kushkevych@mail.muni.cz)[ ](kushkevych@mail.muni.cz)Simon K. -M. R. Rittmann  \n [simon.rittmann@univie.ac.at](simon.rittmann@univie.ac.at)[ ](simon.rittmann@univie.ac.at)RECEIVED 14 August 2024 ACCEPTED 31 October 2024 PUBLISHED 05 December 2024  \nCITATION  \nBychkova S, Bychkov M, Dordević D, Rittmann SK-MR, Vítězová M and Kushkevych I (2024) The impact of 3-sulfo-taurolithocholic acid on ATPase activity in patients’ colorectal cancer and normal colon tissues, and its hepatic effects in rodents.  \nFront. Vet. Sci. 11:1480122 .  \ndoi: 10.3389/fvets.2024.1480122  \nCOPYRIGHT  \n© 2024 Bychkova, Bychkov, Dordević, Rittmann, Vítězová and Kushkevych. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY) . The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.  \nThe impact of 3-sulfo-taurolithocholic acid on ATPase activity in patients’colorectal cancer and normal colon tissues, and its hepatic effects in rodents  \nSolomiia Bychkova 1, Mykola Bychkov 2, Dani Dordević3, Simon K. -M. R. Rittmann4*, Monika Vítězová 5 and Ivan Kushkevych 5*  \n1 Department of Human and Animal Physiology, Faculty of Biology, Ivan Franko National University of Lviv, Lviv, Ukraine, 2 Department of Therapy No. 1, Medical Diagnostic and Hematology and Transfusiology of Faculty of Postgraduate Education, Danylo Halytsky Lviv National Medical University, Lviv, Ukraine, 3 Department of Plant Origin Food Sciences, Faculty of Veterinary Hygiene and Ecology, University of Veterinary Sciences Brno, Brno, Czechia, 4Archaea Physiology & Biotechnology Group, Department of Functional and Evolutionary Ecology, Universität Wien, Wien, Austria, 5 Department of Experimental Biology, Faculty of Science, Masaryk University, Brno, Czechia  \nColorectal cancer is influenced by genetic mutations, lifestyle factors, and diet, particularly high fat intake, which raises bile acid levels in the intestinal lumen. This study hypothesized that bile acids contribute to tumorigenesis by disrupting ion transport and ATPase activity in the intestinal mucosa. The effects of 3-sulfotaurolithocholic acid (TLC–S) on ATPase activity were investigated in colorectal cancer samples from 10 patients, using adjacent healthy tissue as controls, and in rodent liver function. ATPase activity was measured spectrophotometrically by determining inorganic phosphorus (Pi) in postmitochondrial fractions. Ca2+ dynamics were assessed in isolated mouse hepatocytes with fluorescence imaging, and rat liver mitochondria were studied using polarographic methods to evaluate respiration and oxidative phosphorylation. TLC–S increased Na+/K+ ATPase activity by 1.5 times in colorectal cancer samples compared to controls (p ≤ 0.05) . In healthy mucosa, TLC–S decreased Mg2+ ATPase activity by 3.6 times (p ≤ 0.05), while Mg2+ ATPase activity in cancer tissue remained unchanged. TLC–S had no significant effect on Ca2+ ATPase activity in healthy colon mucosa but showed a trend toward decreased activity in cancer tissue. In rat liver, TLC–S decreased Ca2+ ATPase and Na+/K+ ATPase activities while increasing basal Mg2+ ATPase activity (p ≤ 0.05) . Additionally, TLC–S induced cytosolic Ca2+ signals in mouse hepatocytes, partially attenuated by NED-19, an NAADP antagonist (p ≤ 0.05) . TLC–S also ","cbCaiqF5Q2ztwXEr","https://ap.wps.com/l/cbCaiqF5Q2ztwXEr","pdf",892817,12,"English","# Introduction\n## Bile acids, secondary bile acids, and colon carcinogenesis\n## Study hypothesis and investigated ATPase activity\n# Methods\n## Patient tissue ATPase measurements\n## Mouse hepatocyte Ca2+ imaging\n## Rat liver mitochondria respiration and oxidative phosphorylation\n# Results\n## TLC–S effects on Na+/K+ ATPase in cancer vs controls\n## TLC–S effects on Mg2+ and Ca2+ ATPase activities\n## TLC–S cytosolic Ca2+ signals and NED-19 attenuation\n## TLC–S effects on rat liver mitochondrial respiration\n# Discussion\n## Differential modulation of ATPase activity in cancerous vs healthy tissues\n## Mechanistic implications for colorectal cancer and hepatic function","[{\"question\":\"What was the main hypothesis of the study?\",\"answer\":\"The study hypothesized that bile acids can contribute to tumorigenesis by disrupting ion transport and ATPase activity in the intestinal mucosa.\"},{\"question\":\"How did TLC–S affect Na+/K+ ATPase activity in colorectal cancer samples?\",\"answer\":\"TLC–S increased Na+/K+ ATPase activity by about 1.5 times in colorectal cancer samples compared with controls (p ≤ 0.05).\"},{\"question\":\"What hepatic and calcium-related effects were observed in rodents?\",\"answer\":\"In rat liver, TLC–S decreased Ca2+ ATPase and Na+/K+ ATPase activities while increasing basal Mg2+ ATPase activity (p ≤ 0.05). In mouse hepatocytes, TLC–S induced cytosolic Ca2+ signals that were partially attenuated by NED-19.\"}]","The impact of 3-sulfo-taurolithocholic acid on ATPase activity in patients' colorectal cancer and normal colon tissues, and its hepatic effects in rodents | PDF",1790037925]