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Rats receive streptozotocin to induce diabetes, while controls receive buffer; animals are analyzed one week later. Blood glucose and insulin are quantified using ELISA, and pancreatic islets are sectioned and stained to compare beta-cell numbers. Students then present posters combining quantitative insulin and glucose data with histological results, gaining hands-on hypothesis testing experience.",{"@graph":69,"@context":121},[70,84,104],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":35,"@type":76,"position":81},"https://docshare.wps.com/document/healthcare/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/the-development-of-an-inquiry-based-laboratory-module-exploring-the-pathophysiology-of-diabetes-abstract-and-key-results/594829/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":98,"encodingFormat":97,"isAccessibleForFree":99,"interactionStatistic":100},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/the-development-of-an-inquiry-based-laboratory-module-exploring-the-pathophysiology-of-diabetes-abstract-and-key-results/594829.png","ImageObject",300,407,{"name":92,"@type":93},"Mary Man","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-10-15",true,{"@type":101,"interactionType":102,"userInteractionCount":4},"InteractionCounter",{"@type":103},"ViewAction",{"@type":105,"mainEntity":106},"FAQPage",[107,113,117],{"name":108,"@type":109,"acceptedAnswer":110},"What is the purpose of the inquiry-based laboratory module?","Question",{"text":111,"@type":112},"The module teaches students biomedical techniques for tissue sampling, processing, and imaging while helping them understand diabetes pathology through hypothesis testing and data interpretation.","Answer",{"name":114,"@type":109,"acceptedAnswer":115},"How was diabetes induced in the rat model?",{"text":116,"@type":112},"Diabetes was induced using streptozotocin (STZ), a known diabetogen, while control rats received buffer solution injections.",{"name":118,"@type":109,"acceptedAnswer":119},"What measurements were used to evaluate the effects of treatment?",{"text":120,"@type":112},"The study measured blood glucose levels and insulin concentrations using ELISA, and it compared pancreatic islet beta-cell counts using histological processing and hematoxylin/phloxine staining.","https://schema.org",{"og:url":83,"og:type":123,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":125,"canonical":83},"index,follow",{"doc_id":127,"site_id":62},594829,1792079923,{"code":4,"msg":5,"data":130},{"doc_id":127,"user_id":131,"nickname":92,"user_avatar":132,"doc_module":4,"category_id":34,"category_name":35,"doc_title":65,"doc_description":67,"doc_content":133,"file_id":134,"file_url":135,"file_type":136,"file_size":137,"view_count":4,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":34,"language":138,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":139,"faqs":140,"seo_title":141,"seo_description":67,"update_tm":128,"read_time":142},7421720224475,"https://ap-avatar.wpscdn.com/davatar_276721f389ce27ea32af1340a28f341c","ARTICLES  \nThe Development of an Inquiry-Based Laboratory Module Exploring the  \nPathophysiology of Diabetes  \nRachelle M. Belanger*, Gregory M. Grabowski, Gnanada S. Joshi and Jacob E. Tuttle Biology Department, University of Detroit Mercy, Detroit, MI 48221  \nAbstract: Histotechnology is commonly used in medical research, pathological testing, and pharmaceutical development. We designed a three-week, inquiry-based laboratory module that helps prepare students for biomedical careers by teaching them tissue sampling, processing, and imaging. Rats were treated with streptozotocin (a known diabetogen) while control rats were injected with buffer solution. Rats were sacrificed one week following treatment. Pre-and post-injection weights were compared and blood samples were collected for glucose analysis and insulin determinations using an enzyme-linked immunosorbent assay (ELISA) . Pancreatic tissue was collected, preserved in Bouin’s fixative, embedded in paraffin, and sectioned using a microtome. Students then performed hematoxylin/phloxine staining. The number of islet beta cells were compared between control and treated rats.  \nBlood glucose measurements demonstrated that treated rats had significantly higher blood glucose levels and lower beta cells numbers, while the ELISA tests indicated that treated rats had lower blood insulin concentrations. Following this module, students presented an individual poster with images and quantitative data analyses that included insulin concentrations, blood glucose levels, and histological images of pancreatic islets, in addition to beta cell quantification. Overall, students gained hands-on experience with hypothesis testing and an understanding of the pathology of diabetes.  \nKEYWORDS: Rat, diabetes, insulin, β cells, pathophysiology, enzyme-linked immunosorbent assay  \nIntroduction  \nThe National Science Foundation (2011) Vision and Change document calls on scientific educators to actively involve students in their learning process, rather than make them passive learners. Therefore, there is a need for an inquiry-based scientific education (IBSE) where students learn to acquire knowledge on their own, through hands-on experiences. This provides them with an increased understanding of the scientific method, increased scientific literacy, and direct practice in the processes of hypothesis testing (NSF 2014; Riga et al. 2017; Russell et al. 2007) . Undergraduate students that participate in inquiry-based laboratory courses are more likely to be retained and prepared for graduate degrees and professional positions in biomedical sciences (Gregerman et al. 1998; Science 2011; Weaver et al. 2008) . Further, participation in an inquiry-based laboratory experiences by women and underrepresented minorities demonstrably increases the likelihood that they will pursue a graduate or professional programs by 14-17%(Eagan et al. 2013; Gregerman et al. 1998). Including a research component in required coursework can also help increase diversity in scientific research careers, especially when independent research lab positions are not available (Bangera and Brownell 2014) . Therefore, reform efforts in undergraduate STEM have focused on shifting to a learner-centered and applied, hands-on learning environment (NSF 2014; Woodin et al. 2010) .  \nWe developed an inquiry-based research experience that affords students the opportunity to follow the scientific method, formulate hypotheses, perform experiments and collect, interpret and present the scientific data they collected. We used a rat model and induced diabetes using streptozotocin (STZ; a known diabetogen) . STZ destroys β cells in the pancreas and induces type 1 (insulin-dependent) diabetes (Szkudelski 2001). Using this diabetic model, students in our physiology laboratory course were involved in a research experience where they were able to collect both histological and physiological data. This three-week laboratory module allows students to link c","cbCaib0jQa41rZOW","https://ap.wps.com/l/cbCaib0jQa41rZOW","pdf",606942,"English","# Abstract\n# Keywords\n# Introduction","[{\"question\":\"What is the purpose of the inquiry-based laboratory module?\",\"answer\":\"The module teaches students biomedical techniques for tissue sampling, processing, and imaging while helping them understand diabetes pathology through hypothesis testing and data interpretation.\"},{\"question\":\"How was diabetes induced in the rat model?\",\"answer\":\"Diabetes was induced using streptozotocin (STZ), a known diabetogen, while control rats received buffer solution injections.\"},{\"question\":\"What measurements were used to evaluate the effects of treatment?\",\"answer\":\"The study measured blood glucose levels and insulin concentrations using ELISA, and it compared pancreatic islet beta-cell counts using histological processing and hematoxylin/phloxine staining.\"}]","The Development of an Inquiry-Based Laboratory Module Exploring the Pathophysiology of Diabetes - Abstract and Key Results | PDF",18]