[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"doc-seo-333516-105":3,"detail-sidebar-cat-0-en-105":73,"doc-detail-333516-en":122},{"code":4,"msg":5,"data":6},0,"ok",{"site_id":7,"language":8,"slug":9,"title":10,"keywords":11,"description":12,"schema_data":13,"social_meta":66,"head_meta":68,"extra_data":70,"updated_unix":72},105,"en","the-common-bisulfite-conversion-based-techniques-to-analyze-dna-methylation-in-human-cancers","The common bisulfite-conversion-based techniques to analyze DNA methylation in human cancers","","DNA methylation is a key molecular modification influencing cancer gene expression. Evaluation of epigenetic alterations, including hypomethylation and hypermethylation at specific genes, supports cancer diagnosis and helps monitor biomarkers and predict therapy response. Detection methods for DNA methylation status commonly rely on sodium bisulfite treatment. This review summarizes major sodium-bisulfite-based techniques, detailing their advantages, limitations, and practical use in assessing human cancers, and highlights factors guiding technique selection.",{"@graph":14,"@context":65},[15,34,56],{"@type":16,"itemListElement":17},"BreadcrumbList",[18,23,27,31],{"item":19,"name":20,"@type":21,"position":22},"https://docshare.wps.com","Home","ListItem",1,{"item":24,"name":25,"@type":21,"position":26},"https://docshare.wps.com/document/","Document",2,{"item":28,"name":29,"@type":21,"position":30},"https://docshare.wps.com/document/research-report/","Research & Report",3,{"item":32,"name":10,"@type":21,"position":33},"https://docshare.wps.com/document/the-common-bisulfite-conversion-based-techniques-to-analyze-dna-methylation-in-human-cancers/333516/",4,{"url":32,"name":10,"@type":35,"image":36,"author":41,"headline":10,"publisher":44,"fileFormat":47,"inLanguage":8,"description":12,"dateModified":48,"datePublished":49,"encodingFormat":47,"isAccessibleForFree":50,"interactionStatistic":51},"DigitalDocument",{"url":37,"@type":38,"width":39,"height":40},"https://docshare.wps.com/thumbnails/the-common-bisulfite-conversion-based-techniques-to-analyze-dna-methylation-in-human-cancers/333516.png","ImageObject",300,407,{"name":42,"@type":43},"Violet","Person",{"url":19,"name":45,"@type":46},"DocShare","Organization","application/pdf","2026-10-29","2026-09-21",true,{"@type":52,"interactionType":53,"userInteractionCount":55},"InteractionCounter",{"@type":54},"ViewAction",10,{"@type":57,"mainEntity":58},"FAQPage",[59],{"name":60,"@type":61,"acceptedAnswer":62},"How do researchers choose among different sodium-bisulfite-based techniques?","Question",{"text":63,"@type":64},"Selection depends on factors such as genomic DNA quality and quantity, required specificity and sensitivity, simplicity and strength of the technique, and availability of reagents and specific instruments.","Answer","https://schema.org",{"og:url":32,"og:type":67,"og:title":10,"og:site_name":45,"og:description":12},"article",{"robots":69,"canonical":32},"index,follow",{"doc_id":71,"site_id":7},333516,1790013019,{"code":4,"msg":74,"data":75},"success",[76,80,84,88,93,98,103,107,112,115,118],{"id":22,"doc_module":4,"doc_module_name":25,"category_name":77,"show_sort_weight":78,"slug":79},"Story & Novel",90,"story-novel",{"id":26,"doc_module":4,"doc_module_name":25,"category_name":81,"show_sort_weight":82,"slug":83},"Literature",80,"literature",{"id":33,"doc_module":4,"doc_module_name":25,"category_name":85,"show_sort_weight":86,"slug":87},"Exam",70,"exam",{"id":89,"doc_module":4,"doc_module_name":25,"category_name":90,"show_sort_weight":91,"slug":92},5,"Comic",60,"comic",{"id":94,"doc_module":4,"doc_module_name":25,"category_name":95,"show_sort_weight":96,"slug":97},6,"Technology",50,"technology",{"id":99,"doc_module":4,"doc_module_name":25,"category_name":100,"show_sort_weight":101,"slug":102},7,"Healthcare",40,"healthcare",{"id":104,"doc_module":4,"doc_module_name":25,"category_name":29,"show_sort_weight":105,"slug":106},8,30,"research-report",{"id":108,"doc_module":4,"doc_module_name":25,"category_name":109,"show_sort_weight":110,"slug":111},9,"Religion & Spirituality",20,"religion-spirituality",{"id":110,"doc_module":4,"doc_module_name":25,"category_name":113,"show_sort_weight":110,"slug":114},"World Cup","world-cup",{"id":55,"doc_module":4,"doc_module_name":25,"category_name":116,"show_sort_weight":55,"slug":117},"Lifestyle","lifestyle",{"id":119,"doc_module":4,"doc_module_name":25,"category_name":120,"show_sort_weight":89,"slug":121},19,"General","general",{"code":4,"msg":74,"data":123},{"doc_id":71,"user_id":124,"nickname":42,"user_avatar":125,"doc_module":4,"category_id":104,"category_name":29,"doc_title":10,"doc_description":12,"doc_content":126,"file_id":127,"file_url":128,"file_type":129,"file_size":130,"view_count":55,"is_deleted":4,"is_public":22,"is_downloadable":22,"audit_status":22,"page_count":131,"language":132,"language_code":8,"site_id":7,"html_lang":8,"table_of_contents":133,"faqs":134,"seo_title":135,"seo_description":12,"update_tm":136,"read_time":137,"preview_page_count":4},4398048950312,"https://ap-avatar.wpscdn.com/avatar/400002538284de19e3c?_k=1778320343897328908","Jeddi et al. Cancer Cell International (2024) 24:240 [https://doi.org/10.1186/s12935-024-03405-2](https://doi.org/10.1186/s12935-024-03405-2)  \nCancer Cell International  \n REVIEW Open Access  \nThe common bisulfite-conversion-based techniques to analyze DNA methylationin human cancers  \nFarhad Jeddi1,2, Elnaz Faghfuri3, Sahar Mehranfar4 and Narges Soozangar1,3*  \nAbstract  \nDNA methylation is an important molecular modification that plays a key role in the expression of cancer genes. Evaluation of epigenetic changes, hypomethylation and hypermethylation, in specific genes are applied for cancer diagnosis. Numerous studies have concentrated on describing DNA methylation patterns as biomarkers for cancer diagnosis monitoring and predicting response to cancer therapy. Various techniques for detecting DNA methylation status in cancers are based on sodium bisulfite treatment. According to the application of these methods in research and clinical studies, they have a number of advantages and disadvantages. The current review highlights sodium bisulfite treatment-based techniques, as well as, the advantages, drawbacks, and applications of these methods in the evaluation of human cancers.  \nKeywords Bisulfite-conversion, Cancer, DNA methylation, Epigenetic  \nIntroduction  \nDNA methylation is an epigenetic modification that occurs by adding methyl groups to the fifth carbon of cytosine (5mc). DNA methylation has been associated with not only human cancer but also with numerous cellular processes such as embryonic development, transposon inactivation, genomic imprinting, X chromosome inactivation, chromatin structure alteration, and transcriptional repression [1–3]. Methylation modifications are predictive indicators and powerful prognostic markers in the diagnosis and treatment of cancers [4–8].  \n*Correspondence: Narges Soozangar  \n[n.soozangar@arums.ac.ir](n.soozangar@arums.ac.ir); [soozangar. narges@gmail.com](soozangar. narges@gmail.com)  \n1 Zoonoses Research Center, Ardabil University of Medical Sciences, Ardabil, Iran  \n2 Department of Genetics and Pathology, School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran  \n3 Digestive Diseases Research Center, Ardabil University of Medical Sciences, Ardabil, Iran  \n4 Department of Genetics and Immunology, Faculty of Medicine, Urmia University of Medical Sciences, Urmia, Iran  \nIn this review, we will describe each of the techniques as well as focus on their application in cancer research and diagnosis. Furthermore, the pros and cons of each method will be represented in detail. Not all methods based on sodium bisulfite treatment can be covered in this review; therefore, we will emphasize approaches that are easy to use, readily accessible, and most robust for research centers. In addition, there are several important factors in choosing a technique for DNA methylation study, including the quality and quantity of genomic DNA (gDNA) samples, the specificity and sensitivity required to analyze, the simplicity and strength of the technique, and the accessibility of reagents and specific devices [9].  \nIn this study, we will explain methodologies developed to analyze epigenetic alterations using sodium bisulfite (NaHSO3) treatment platforms, including; MSP (methylation-specific PCR), MS-HRM (methylation-specific high-resolution melting), pyrosequencing, MS-SSCA (methylation-specific single-strand conformation analysis), MS-SNuPE (methylation-specific single nucleotide  \n© The Author(s) 2024. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated o","cbCaitbmSE6V1VER","https://ap.wps.com/l/cbCaitbmSE6V1VER","pdf",1356774,13,"English","# Abstract\n# Introduction\n## DNA methylation and cancer relevance\n## Selection factors for methylation techniques\n# Sodium bisulfite treatment and core concepts\n# Techniques covered (overview)\n## MSP (methylation-specific PCR)\n## Other bisulfite-based methods referenced","[{\"question\":\"How do researchers choose among different sodium-bisulfite-based techniques?\",\"answer\":\"Selection depends on factors such as genomic DNA quality and quantity, required specificity and sensitivity, simplicity and strength of the technique, and availability of reagents and specific instruments.\"}]","The common bisulfite-conversion-based techniques to analyze DNA methylation in human cancers | PDF",1790002842,33]