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This study evaluates the clinical relevance of Notch4 receptor expression in colon adenocarcinoma using immunohistochemistry and fluorescence, complemented by immunogold labelling and TEM to determine intracellular localisation. Among 129 tumours, strong Notch4 expression predominates and is associated with adverse clinicopathological features, including higher histological grade, elevated PCNA expression, deeper invasion, and angioinvasion. High Notch4 correlates with reduced 5-year survival, supporting poor prognosis.",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":35,"@type":76,"position":81},"https://docshare.wps.com/document/healthcare/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/the-clinical-application-of-immunohistochemical-expression-of-notch4-protein-in-patients-with-colon-adenocarcinoma/381072/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/the-clinical-application-of-immunohistochemical-expression-of-notch4-protein-in-patients-with-colon-adenocarcinoma/381072.png","ImageObject",300,407,{"name":92,"@type":93},"Noah","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-11-01","2026-09-24",true,{"@type":102,"interactionType":103,"userInteractionCount":39},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"What is the study’s main objective regarding Notch4 in colon adenocarcinoma?","Question",{"text":112,"@type":113},"To evaluate the clinical significance of Notch4 receptor expression and its potential prognostic value in patients with colon adenocarcinoma.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"How was Notch4 expression measured in the study?",{"text":117,"@type":113},"Notch4 expression was assessed by immunohistochemical and fluorescence expression using a Notch4 antibody, with intracellular localisation examined using immunogold labelling and TEM.",{"name":119,"@type":110,"acceptedAnswer":120},"What clinical factors were associated with high Notch4 expression?",{"text":121,"@type":113},"High Notch4 expression correlated with tumour histological grade, PCNA immunohistochemical expression, depth of invasion, and angioinvasion.","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},381072,1791171206,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":34,"category_name":35,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":39,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":139,"language":140,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":141,"faqs":142,"seo_title":143,"seo_description":67,"update_tm":144,"read_time":145},8796095462418,"https://ap-avatar.wpscdn.com/avatar/80000253c1241d02b47?x-image-process=image/resize,m_fixed,w_180,h_180&k=1778826106357471780","Article  \nThe Clinical Application of Immunohistochemical Expression of Notch4 Protein in Patients with Colon Adenocarcinoma  \nMarlena Brzozowa-Zasada 1,*, Adam Piecuch 1, Marek Michalski 1,2, Natalia Matysiak 1, Marek Kucharzewski 3 and Marek J. Łos 4,5  \nCitation: Brzozowa-Zasada, M.; Piecuch, A.; Michalski, M.; Matysiak, N.; Kucharzewski, M.; Łos, M.J. The Clinical Application of Immunohistochemical Expression of Notch4 Protein in Patients with Colon Adenocarcinoma. Int. J. Mol. Sci. 2023, 24, 7502. [https://doi.org/](https://doi.org/)[ ](https://doi.org/)[10.3390/ijms24087502](10.3390/ijms24087502)  \nAcademic Editors: Sergio Crovella and Paola Maura Ticarico  \nReceived: 28 February 2023  \nRevised: 12 April 2023  \nAccepted: 14 April 2023  \nPublished: 19 April 2023  \nCopyright: © 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license ([https://](https://)[ ](https://)[creativecommons.org/licenses/by/](creativecommons.org/licenses/by/)[ ](creativecommons.org/licenses/by/)[4.0/](4.0/)) .  \n1 Department of Histology and Cell Pathology in Zabrze, Faculty of Medical Sciences in Zabrze, Medical University of Silesia in Katowice, 40-055 Katowice, Poland  \n2 Silesian Nanomicroscopy Centre in Zabrze, Silesia LabMed-Research and Implementation Centre, Medical University of Silesia, 40-055 Katowice, Poland  \n3 Faculty of Health Sciences, Jan Dlugosz University of Czestochowa, 42-200 Czestochowa, Poland  \n4 Department of Pathology, Pomeranian Medical University, 71-344 Szczecin, Poland  \n5 Autophagy Research Center, Shiraz University of Medical Sciences, Shiraz 7134845794, Iran  \n* [Correspondence: mbrzozowa@sum.edu.pl or marlena.brzozowa@op.pl](Correspondence: mbrzozowa@sum.edu.pl or marlena.brzozowa@op.pl)  \nAbstract: The Notch signalling pathway is one of the most conserved and well-characterised pathways involved in cell fate decisions and the development of many diseases, including cancer. Among them, it is worth noting the Notch4 receptor and its clinical application, which may have prognostic value in patients with colon adenocarcinoma. The study was performed on 129 colon adenocarcinomas. Immunohistochemical and fluorescence expression of Notch4 was performed using the Notch4 antibody. The associations between the IHC expression of Notch4 and clinical parameters were analysed using the Chi2 test or Chi2Yatesa test. The Kaplan–Meier analysis and the log-rank test were used to verify the relationship between the intensity of Notch4 expression and the 5-year survival rate of patients. Intracellular localisation of Notch4 was detected by the use of the immunogold labelling method and TEM. 101 (78.29%) samples had strong Notch4 protein expression, and 28 (21.71%) samples were characterised by low expression. The high expression of Notch4 was clearly correlated with the histological grade of the tumour (p \u003C 0.001), PCNA immunohistochemical expression (p \u003C 0.001), depth of invasion (p \u003C 0.001) and angioinvasion (p \u003C 0.001) . We can conclude that high expression of Notch4 is correlated with poor prognosis of colon adenocarcinoma patients (log-rank, p \u003C 0.001) .  \nKeywords: Notch4; colon adenocarcinoma; 5-year survival rate; immunohistochemistry (IHC); immunogold labelling  \n1. Introduction  \nColorectal cancer (CRC) is the third most common cancer worldwide [1] . Important factors associated with the development of this type of cancer include drinking alcohol, smoking, unhealthy dietary patterns and obesity [2] . Among factors which are clearly correlated with disease development, it should also be mentioned about ageing, genetic mutations and hereditary factors [3] . The development of colorectal cancer occurs gradually as a sequence of specific morphological and genetic changes. One of the most common types of colorectal cancer is adenocarcinoma (COAD) . This type of cancer develops from colorectal glandu","cbCaihkE5rgaUPj4","https://ap.wps.com/l/cbCaihkE5rgaUPj4","pdf",9182095,17,"English","# Abstract\n# Introduction\n# Materials and Methods\n# Results\n# Discussion\n# Conclusions","[{\"question\":\"What is the study’s main objective regarding Notch4 in colon adenocarcinoma?\",\"answer\":\"To evaluate the clinical significance of Notch4 receptor expression and its potential prognostic value in patients with colon adenocarcinoma.\"},{\"question\":\"How was Notch4 expression measured in the study?\",\"answer\":\"Notch4 expression was assessed by immunohistochemical and fluorescence expression using a Notch4 antibody, with intracellular localisation examined using immunogold labelling and TEM.\"},{\"question\":\"What clinical factors were associated with high Notch4 expression?\",\"answer\":\"High Notch4 expression correlated with tumour histological grade, PCNA immunohistochemical expression, depth of invasion, and angioinvasion.\"}]","The Clinical Application of Immunohistochemical Expression of Notch4 Protein in Patients with Colon Adenocarcinoma | PDF",1790243446,43]