[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"detail-sidebar-cat-0-en-105":3,"doc-seo-385263-105":59,"doc-detail-385263-en":130},{"code":4,"msg":5,"data":6},0,"success",[7,13,18,23,28,33,38,43,48,51,55],{"id":8,"doc_module":4,"doc_module_name":9,"category_name":10,"show_sort_weight":11,"slug":12},1,"Document","Story & Novel",90,"story-novel",{"id":14,"doc_module":4,"doc_module_name":9,"category_name":15,"show_sort_weight":16,"slug":17},2,"Literature",80,"literature",{"id":19,"doc_module":4,"doc_module_name":9,"category_name":20,"show_sort_weight":21,"slug":22},4,"Exam",70,"exam",{"id":24,"doc_module":4,"doc_module_name":9,"category_name":25,"show_sort_weight":26,"slug":27},5,"Comic",60,"comic",{"id":29,"doc_module":4,"doc_module_name":9,"category_name":30,"show_sort_weight":31,"slug":32},6,"Technology",50,"technology",{"id":34,"doc_module":4,"doc_module_name":9,"category_name":35,"show_sort_weight":36,"slug":37},7,"Healthcare",40,"healthcare",{"id":39,"doc_module":4,"doc_module_name":9,"category_name":40,"show_sort_weight":41,"slug":42},8,"Research & Report",30,"research-report",{"id":44,"doc_module":4,"doc_module_name":9,"category_name":45,"show_sort_weight":46,"slug":47},9,"Religion & Spirituality",20,"religion-spirituality",{"id":46,"doc_module":4,"doc_module_name":9,"category_name":49,"show_sort_weight":46,"slug":50},"World Cup","world-cup",{"id":52,"doc_module":4,"doc_module_name":9,"category_name":53,"show_sort_weight":52,"slug":54},10,"Lifestyle","lifestyle",{"id":56,"doc_module":4,"doc_module_name":9,"category_name":57,"show_sort_weight":24,"slug":58},19,"General","general",{"code":4,"msg":60,"data":61},"ok",{"site_id":62,"language":63,"slug":64,"title":65,"keywords":66,"description":67,"schema_data":68,"social_meta":123,"head_meta":125,"extra_data":127,"updated_unix":129},105,"en","the-chromatin-architectural-regulator-snd1-mediates-metastasis-in-triple-negative-breast-cancer-by-promoting-cdh1-gene-methylation","The chromatin architectural regulator SND1 mediates metastasis in triple-negative breast cancer by promoting CDH1 gene methylation","","Background: SND1 contributes to tumorigenesis, invasion, and metastasis across cancers, and prior work suggests it promotes breast cancer cell invasion and migration. Triple-negative breast cancer (TNBC) shows high metastatic potential and poor prognosis, yet the specific roles and mechanisms of SND1 in TNBC metastasis are unclear. Methods: SND1 expression was examined in TNBC tissues (n=58) using immunostaining, with prognosis assessed by Kaplan–Meier analysis. Mechanisms were tested via qRT-PCR, Western blot, immunofluorescence, ChIP and dual-luciferase assays, and methylation analyses by bisulfite sequencing and MeDIP, alongside migration and invasion functional assays. Results and Conclusion: SND1 overexpression correlates with worse TNBC prognosis, and SND1 knockdown suppresses migration and invasion; SND1 drives TNBC metastasis via epigenetic chromatin regulation through DNMT3A-mediated CCND1 methylation and CDH1 suppression.",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":35,"@type":76,"position":81},"https://docshare.wps.com/document/healthcare/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/the-chromatin-architectural-regulator-snd1-mediates-metastasis-in-triple-negative-breast-cancer-by-promoting-cdh1-gene-methylation/385263/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/the-chromatin-architectural-regulator-snd1-mediates-metastasis-in-triple-negative-breast-cancer-by-promoting-cdh1-gene-methylation/385263.png","ImageObject",300,407,{"name":92,"@type":93},"Elsa","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-10-29","2026-09-24",true,{"@type":102,"interactionType":103,"userInteractionCount":39},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"What role does SND1 play in triple-negative breast cancer metastasis according to the study?","Question",{"text":112,"@type":113},"SND1 promotes TNBC invasion and migration. It advances a metastatic phenotype by epigenetically altering chromatin interactions and triggering downstream methylation events.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"How was SND1 expression measured and how was clinical relevance assessed?",{"text":117,"@type":113},"Immunostaining was used to detect SND1 expression in TNBC tissue samples, and patient prognosis association was evaluated using Kaplan–Meier analysis.",{"name":119,"@type":110,"acceptedAnswer":120},"Which molecular pathway does the study propose connecting SND1 to methylation and metastasis?",{"text":121,"@type":113},"The study links SND1-driven chromatin changes to DNMT3A transcription activation. DNMT3A then induces CCND1 gene methylation, resulting in TNBC metastasis, alongside suppression of CDH1 activity.","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},385263,1790502972,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":34,"category_name":35,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":39,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":139,"language":140,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":141,"faqs":142,"seo_title":143,"seo_description":67,"update_tm":144,"read_time":145},137455077381,"https://ap-avatar.wpscdn.com/davatar_994ba38a5ba835b3df7d355c54d3ed8d","Zhang et al. Breast Cancer Research (2023) 25:129 [https://doi.org/10.1186/s13058-023-01731-3](https://doi.org/10.1186/s13058-023-01731-3)  \nBreast Cancer Research  \n RESEARCH Open Access  \nThe chromatin architectural regulator SND1   mediates metastasis in triple-negative breast cancer by promoting CDH1 gene methylation  \nHuibian Zhang1,2,3†, Min Gao1,2,3†, Wenying Zhao1,2,3† and Lin Yu1,2,3*  \nAbstract  \nBackground SND1 participates in tumorigenesis, tumour invasion and metastasis in different cancers. Previous studies have shown that SND1 can promote the invasion and migration of breast cancer cells. Triple-negative breast cancer (TNBC) is a specific breast cancer subtype with high metastatic potential and poor prognosis. However, the specific roles and mechanisms of SND1 inTNBC metastasis remain unaddressed.  \nMethods Immunostaining was used to detect the SND1 expression in tissue samples of 58 TNBC and 10 glioblastomas (GBM) as positive control. The correlation between SND1 expression and patient prognosis was assessed using the Kaplan–Meier estimator. The gene expression was evaluated by qRT-PCR, Western blot and immunofluorescence analyses. Gene Ontology analysis, ChIP, a dual-luciferase reporter assay, EMSA, and 3C analysis were applied to identify SND1-activated target genes. Bisulfite sequencing PCR and MeDIP were used to detect DNA methylation. We also used wound healing, Transwell and orthotopic implantation assays to investigate the function of SND1 inTNBC cell migration and invasion.  \nResults The data of immunohistochemistry manifested that SND1 is the overexpression in metastasized TNBC and an independent factor forTNBC prognosis. SND1 knockdown inhibited the migration and invasion ofTNBC cells. We found that SND1 promotes the metastatic phenotype ofTNBC cells by epigenetically altering chromatin conformational interactions, which in turn activates DNMT3A transcription. Then, DNMT3A attenuates CCND1 expression by inducing CCND1 gene methylation, leading toTNBC metastasis.  \nConclusion SND1 can promote the invasion and migration ofTNBC cells by promoting DNMT3A expression and suppressing CDH1 activity. SND1 is a potential biomarker and a promising therapeutic target forTNBC.  \nKeywords SND1, Triple-negative breast cancer, Chromatin architectural interaction, Metastasis  \n†Huibian Zhang, Min Gao and Wenying Zhao contributed equally to this work.  \n*Correspondence:  \nLin Yu [onoblivion@tmu.edu.cn](onoblivion@tmu.edu.cn)  \n1 Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Tianjin Medical University, Tianjin 300070, China  \n2 Laboratory of Molecular Immunology, Research Center of Basic Medical Science, Tianjin Medical University, Tianjin 300070, China  \n3 Tianjin Key Laboratory of Cellular and Molecular Immunology and Key Laboratory of the Educational Ministry of China, Tianjin Medical University, Tianjin 300070, China  \nBackground  \nBreast cancer has the highest case morbidity and mortality in female cancer patients among the world [1, 2]. Triple-negative breast cancers (TNBC) is one of the most malignant and aggressive breast cancer [3, 4]. TNBC is highly invasive, and approximately 46% of TNBCs undergo distant metastasis, which is also the main factor for the mortality of patients [5, 6]. Due to the relationship between metastasis and TNBC prognosis, further investigation of the detail mechanism regulating TNBC metastasis is absolutely imperative.  \n© The Author(s) 2023. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line","cbCaiaWoSOfzyAd0","https://ap.wps.com/l/cbCaiaWoSOfzyAd0","pdf",11982388,17,"English","# Abstract\n## Background\n## Methods\n## Results\n## Conclusion\n# Introduction\n## Breast cancer burden and TNBC features\n## Known roles of SND1 in other cancers\n## Rationale for studying SND1 in TNBC","[{\"question\":\"What role does SND1 play in triple-negative breast cancer metastasis according to the study?\",\"answer\":\"SND1 promotes TNBC invasion and migration. It advances a metastatic phenotype by epigenetically altering chromatin interactions and triggering downstream methylation events.\"},{\"question\":\"How was SND1 expression measured and how was clinical relevance assessed?\",\"answer\":\"Immunostaining was used to detect SND1 expression in TNBC tissue samples, and patient prognosis association was evaluated using Kaplan–Meier analysis.\"},{\"question\":\"Which molecular pathway does the study propose connecting SND1 to methylation and metastasis?\",\"answer\":\"The study links SND1-driven chromatin changes to DNMT3A transcription activation. DNMT3A then induces CCND1 gene methylation, resulting in TNBC metastasis, alongside suppression of CDH1 activity.\"}]","The chromatin architectural regulator SND1 mediates metastasis in triple-negative breast cancer by promoting CDH1 gene methylation | PDF",1790267607,43]